FastQCFastQC Report
Thu 26 May 2016
SRR938341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923277
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91650.31351801420118586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67380.23049474955674745No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59850.20473598636051252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.16987784599269928No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31870.10902148513466223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119950.027.0151231
TACATGG119350.026.4815522
GTATCAA163400.025.4017961
ACATGGG127150.024.2963623
TATCAAC189400.021.7562162
GAGTACT83150.021.65163812-13
ATCAACG191750.021.5391543
TCAACGC193150.021.333824
CAACGCA193600.021.2842315
GGTATCA120550.021.1487581
AGTACTT87400.021.0607612-13
AACGCAG198250.020.8297426
AGAGTAC147350.020.59969710-11
GTACTTT88750.020.0993214-15
CATGGGG64200.020.0621974
ACCGTAC1456.145099E-419.6570958
ACGCAGA218600.018.8627787
CGCAGAG221350.018.6284318
CATGGGA65650.018.388374
GTGTAGC17200.018.2857931
Produced by FastQC (version 0.11.5)