##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938341_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2923277 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06856483323339 31.0 31.0 34.0 28.0 34.0 2 31.211856077956348 31.0 31.0 34.0 28.0 34.0 3 31.270888458397888 31.0 31.0 34.0 27.0 34.0 4 34.926669624534384 37.0 35.0 37.0 32.0 37.0 5 34.9365068722533 37.0 35.0 37.0 32.0 37.0 6 34.93963623700388 37.0 35.0 37.0 32.0 37.0 7 34.85481533224529 37.0 35.0 37.0 32.0 37.0 8 34.814337471269404 37.0 35.0 37.0 32.0 37.0 9 36.29299036663306 39.0 35.0 39.0 32.0 39.0 10-11 36.14509418710577 39.0 35.0 39.0 31.0 39.0 12-13 35.97221046790982 38.0 35.0 39.0 30.0 39.0 14-15 36.93568809934878 39.0 36.0 41.0 31.0 41.0 16-17 36.76032206321878 39.0 36.0 41.0 30.0 41.0 18-19 36.623314691012865 39.0 36.0 40.0 30.0 41.0 20-21 36.60574827496676 39.0 36.0 40.5 30.0 41.0 22-23 36.70710524524361 39.0 36.0 41.0 30.0 41.0 24-25 36.61518562900471 39.0 36.0 41.0 30.0 41.0 26-27 36.41637928940706 39.0 35.5 41.0 29.5 41.0 28-29 36.22411594932673 39.0 35.0 40.0 29.0 41.0 30-31 35.96714320948716 38.5 35.0 40.0 28.0 41.0 32-33 35.665318921197 38.0 34.5 40.0 27.0 41.0 34-35 35.386127281130044 38.0 34.0 40.0 26.5 41.0 36-37 35.09099189026561 38.0 34.0 40.0 25.5 41.0 38-39 34.76998228358106 38.0 33.5 40.0 25.0 41.0 40-41 34.667568280392175 38.0 33.0 40.0 24.5 41.0 42-43 34.43059946081059 38.0 33.0 40.0 24.0 41.0 44-45 34.11080920487521 38.0 33.0 40.0 23.5 41.0 46-47 33.70109606445095 38.0 33.0 40.0 21.5 41.0 48-49 33.30444121443161 37.5 32.0 40.0 18.5 41.0 50-51 32.51949524454918 36.5 30.5 39.5 16.5 40.5 52-53 32.53477604072416 37.0 31.0 39.0 15.5 40.0 54-55 32.59422935288035 37.0 31.0 40.0 14.0 41.0 56-57 32.24640771298786 36.5 31.0 39.5 10.5 41.0 58-59 31.788466334185916 36.0 30.5 39.0 8.0 41.0 60-61 31.26209182366228 35.5 29.5 39.0 4.5 40.5 62-63 30.65394641014177 35.0 28.5 39.0 2.0 40.0 64-65 30.138055682030817 35.0 28.0 38.0 2.0 40.0 66-67 29.543283958379583 34.0 27.5 38.0 2.0 40.0 68-69 28.893018862051047 34.0 26.0 37.5 2.0 39.5 70-71 28.217846615288252 34.0 26.0 37.0 2.0 39.0 72-73 27.54374354534312 33.0 24.5 36.0 2.0 39.0 74-75 26.81082206715272 32.5 22.5 35.5 2.0 38.5 76-77 26.032292013380875 32.0 20.5 35.0 2.0 37.5 78-79 25.200785283091545 31.0 18.0 35.0 2.0 37.0 80-81 24.38900384739455 31.0 10.5 35.0 2.0 36.0 82-83 23.620506027995294 30.5 4.5 34.0 2.0 36.0 84-85 22.841046024718153 30.0 2.0 34.0 2.0 35.0 86-87 22.079280889221238 29.0 2.0 34.0 2.0 35.0 88-89 21.278521330684708 29.0 2.0 34.0 2.0 35.0 90-91 20.427337710384613 27.5 2.0 34.0 2.0 35.0 92-93 19.536696659262873 26.0 2.0 34.0 2.0 35.0 94-95 18.612970306953464 25.0 2.0 33.0 2.0 35.0 96-97 17.530723568105245 22.0 2.0 33.0 2.0 35.0 98-99 16.289650963627466 9.5 2.0 32.0 2.0 34.0 100-101 13.584544502624965 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7088500581499133 1101 2 0.6434098889030615 1101 3 0.6623746549668716 1101 4 0.5668289332818119 1101 5 0.6718100038774324 1101 6 0.6246460897386754 1101 7 0.6625350462678767 1101 8 0.5720713149210113 1101 9 0.6189983052498675 1101 10-11 0.8320593044047939 1101 12-13 0.9585276774269929 1101 14-15 1.0896423642474033 1101 16-17 1.1633617481743244 1101 18-19 1.1039662658414358 1101 20-21 1.0381314076881623 1101 22-23 1.1690394267990598 1101 24-25 1.1192466339019376 1101 26-27 1.176252133103361 1101 28-29 1.2271508176983943 1101 30-31 1.3574695704032607 1101 32-33 1.401686544825104 1101 34-35 1.5413855069416442 1101 36-37 1.5326297587314102 1101 38-39 1.6728586929469103 1101 40-41 1.8137267516291544 1101 42-43 1.9304012780024777 1101 44-45 1.8904602935088732 1101 46-47 2.1299305026164177 1101 48-49 2.171199031950671 1101 50-51 2.1961269955890685 1101 52-53 2.4201924620845503 1101 54-55 2.654635460209704 1101 56-57 2.7375055016366474 1101 58-59 2.9363739565578335 1101 60-61 3.123783795745709 1101 62-63 3.0828336464046266 1101 64-65 3.1670092738854443 1101 66-67 3.2924857373047196 1101 68-69 3.4546242526218265 1101 70-71 3.507712774069063 1101 72-73 3.669877418089442 1101 74-75 3.8248861414467754 1101 76-77 4.192751933324693 1101 78-79 4.359191035168973 1101 80-81 4.754403940255543 1101 82-83 4.82880178362835 1101 84-85 5.147892427111287 1101 86-87 5.272714933565993 1101 88-89 5.64728871110373 1101 90-91 5.991309738775126 1101 92-93 6.1209943457674925 1101 94-95 6.380564813837147 1101 96-97 6.288343306839085 1101 98-99 6.36357792312721 1101 100-101 6.1682054042713705 1102 1 0.6702162893487795 1102 2 0.6451295404185693 1102 3 0.6051475834947304 1102 4 0.6000364751711231 1102 5 0.5514801941038598 1102 6 0.5140678413331159 1102 7 0.46607446512539497 1102 8 0.5632173612681513 1102 9 0.7122226772838403 1102 10-11 0.7356083277666343 1102 12-13 0.7173675423533084 1102 14-15 1.1163858203042523 1102 16-17 1.0525586525980586 1102 18-19 1.0678937193947462 1102 20-21 1.0106128490507515 1102 22-23 0.8701781648180784 1102 24-25 0.7067312014988829 1102 26-27 0.8822542486385458 1102 28-29 0.9883250860228046 1102 30-31 1.1136082161935903 1102 32-33 1.1563964092871046 1102 34-35 1.1107036316589927 1102 36-37 1.277528526619811 1102 38-39 1.2929604752503252 1102 40-41 1.510902963752585 1102 42-43 1.6389094694997581 1102 44-45 1.6894861041831106 1102 46-47 1.8481076313021418 1102 48-49 1.85780043039194 1102 50-51 2.134333204932915 1102 52-53 2.08790281822084 1102 54-55 2.164390201877154 1102 56-57 2.25387634745001 1102 58-59 2.509687427861035 1102 60-61 2.431580681469285 1102 62-63 2.7187967269444364 1102 64-65 2.8205387384057055 1102 66-67 2.806838301484756 1102 68-69 3.0415981913400074 1102 70-71 3.297071937370447 1102 72-73 3.3120356583407116 1102 74-75 3.4551901971554457 1102 76-77 3.6007889952622953 1102 78-79 3.928225731599852 1102 80-81 4.331916551732991 1102 82-83 4.570580168913995 1102 84-85 4.554262058050256 1102 86-87 4.875970415289128 1102 88-89 5.234814749805427 1102 90-91 5.593007382618477 1102 92-93 5.572103920440604 1102 94-95 5.929803609165159 1102 96-97 6.054086393345138 1102 98-99 6.126502755477727 1102 100-101 5.91190392714949 1103 1 0.5808955704271135 1103 2 0.5514404316248935 1103 3 0.4754995496493102 1103 4 0.5070521939386907 1103 5 0.503713114235147 1103 6 0.5637463822385556 1103 7 0.5757172840051368 1103 8 0.488496956320688 1103 9 0.5833901544879367 1103 10-11 0.5701066099099776 1103 12-13 0.7489901818597602 1103 14-15 0.9133514538761105 1103 16-17 1.015694202079814 1103 18-19 1.0726058395913896 1103 20-21 1.0455374644225728 1103 22-23 1.0611770777961524 1103 24-25 0.9195112796450928 1103 26-27 1.0257435278725637 1103 28-29 1.1749239328598478 1103 30-31 1.2362205104018003 1103 32-33 1.213359880556112 1103 34-35 1.2341631743368353 1103 36-37 1.3102140945202692 1103 38-39 1.4463706029484982 1103 40-41 1.4263920844829485 1103 42-43 1.4663644904207729 1103 44-45 1.5541000181616127 1103 46-47 1.7165996256449318 1103 48-49 1.659454457534224 1103 50-51 1.792285513925009 1103 52-53 1.840114898057756 1103 54-55 1.9288291992535278 1103 56-57 2.142776787101951 1103 58-59 2.4300673522978435 1103 60-61 2.55321920558508 1103 62-63 2.648036283682135 1103 64-65 2.894300355279885 1103 66-67 2.9647303154937354 1103 68-69 3.079167897477177 1103 70-71 3.036906362210118 1103 72-73 3.2339189213828945 1103 74-75 3.475219322200637 1103 76-77 3.713030185468142 1103 78-79 3.8128169144920214 1103 80-81 4.064144085675519 1103 82-83 4.313561735037656 1103 84-85 4.424497592513305 1103 86-87 4.6148173110392285 1103 88-89 4.918310851055992 1103 90-91 5.093564163370264 1103 92-93 5.136863973314291 1103 94-95 5.210703870745096 1103 96-97 5.486311652466803 1103 98-99 5.538992842884657 1103 100-101 5.539787079141282 1104 1 0.6095776303340728 1104 2 0.5536358013397056 1104 3 0.5009911641485445 1104 4 0.45269009179406794 1104 5 0.44886898222006266 1104 6 0.5064533892318366 1104 7 0.4184783007313655 1104 8 0.5607629584530898 1104 9 0.6145700479423084 1104 10-11 0.6493622783667092 1104 12-13 0.685175327037733 1104 14-15 0.783013561897441 1104 16-17 0.9260259188524032 1104 18-19 0.9662305689597162 1104 20-21 0.9817437144400643 1104 22-23 1.0107487544951965 1104 24-25 1.0098764889817815 1104 26-27 1.0536192896290117 1104 28-29 1.0497187145308686 1104 30-31 1.1918857722557235 1104 32-33 1.1959818525363488 1104 34-35 1.3106678677732972 1104 36-37 1.3645406332612922 1104 38-39 1.4102789953520585 1104 40-41 1.5306892863310324 1104 42-43 1.5522097660616367 1104 44-45 1.5763783333937553 1104 46-47 1.778577659821245 1104 48-49 1.77345009104787 1104 50-51 1.9123258766390734 1104 52-53 1.8174702214678504 1104 54-55 2.0908854495975078 1104 56-57 2.221280726827459 1104 58-59 2.4061750415487886 1104 60-61 2.44124170777485 1104 62-63 2.6736320231630692 1104 64-65 2.7563834938890075 1104 66-67 2.7058184763550592 1104 68-69 3.1544166780605423 1104 70-71 3.102837531049765 1104 72-73 3.2770453200157696 1104 74-75 3.3880949300433336 1104 76-77 3.643083033851905 1104 78-79 3.7049843605219372 1104 80-81 4.2636149022299215 1104 82-83 4.438030149604803 1104 84-85 4.5484884080602725 1104 86-87 4.571739884962131 1104 88-89 5.130411922948635 1104 90-91 5.383620565762364 1104 92-93 5.379049002823752 1104 94-95 5.524582506490962 1104 96-97 5.674913360069862 1104 98-99 5.646716841022769 1104 100-101 5.327235109931491 1105 1 0.5009018130541563 1105 2 0.4008338410631964 1105 3 0.4178261675684034 1105 4 0.41167701664349465 1105 5 0.43141109961087665 1105 6 0.534627051668501 1105 7 0.41048114286668635 1105 8 0.4598917244484042 1105 9 0.5297276602830223 1105 10-11 0.5575324213367381 1105 12-13 0.5839345490363925 1105 14-15 0.6977657715248426 1105 16-17 0.8310650646180875 1105 18-19 0.830422320126786 1105 20-21 0.8211776779571309 1105 22-23 0.8240636272299611 1105 24-25 0.7811254348019219 1105 26-27 0.8657501606638007 1105 28-29 0.9331755612215744 1105 30-31 0.9131968067797018 1105 32-33 0.8245546092083913 1105 34-35 1.078325629525331 1105 36-37 1.2742351037143749 1105 38-39 1.1210163568145646 1105 40-41 1.2124534687855046 1105 42-43 1.4644794236108964 1105 44-45 1.4917637149902632 1105 46-47 1.613763361490001 1105 48-49 1.5805142582291793 1105 50-51 1.5191081544644476 1105 52-53 1.7514510038519262 1105 54-55 1.97131555498229 1105 56-57 2.1015793502511713 1105 58-59 2.0192820665370057 1105 60-61 2.3204861513338955 1105 62-63 2.523954087562668 1105 64-65 2.730148526327863 1105 66-67 2.8536101770542963 1105 68-69 2.938612280719397 1105 70-71 3.11093164025948 1105 72-73 3.2205303224395507 1105 74-75 3.311379854109184 1105 76-77 3.4286983760702334 1105 78-79 3.7863590774704896 1105 80-81 4.176643569281737 1105 82-83 4.324964627760867 1105 84-85 4.5675795224030935 1105 86-87 4.893594444663574 1105 88-89 5.216771997587891 1105 90-91 5.168939409517964 1105 92-93 5.1437484204516295 1105 94-95 5.452826226235164 1105 96-97 5.7055008453777525 1105 98-99 5.902131507237879 1105 100-101 5.431041778324689 1106 1 0.3368007297979929 1106 2 0.35860709743677077 1106 3 0.19131277871694863 1106 4 0.37679984136310907 1106 5 0.2915127316312649 1106 6 0.1583304578790532 1106 7 0.4009537771230711 1106 8 0.35111216060532513 1106 9 0.3990426675464036 1106 10-11 0.3309555816212537 1106 12-13 0.1681953603002242 1106 14-15 0.706169774848405 1106 16-17 0.44004123209738566 1106 18-19 0.53559237618704 1106 20-21 0.44651271732765707 1106 22-23 0.4017236124708532 1106 24-25 0.549555393788431 1106 26-27 0.5925115997979873 1106 28-29 0.4286044437008414 1106 30-31 0.7617264837500812 1106 32-33 0.8639795020831968 1106 34-35 0.774061145081582 1106 36-37 0.7636520859757994 1106 38-39 0.7345542929078732 1106 40-41 0.8018246469358346 1106 42-43 0.4487916262022509 1106 44-45 0.7316863126808144 1106 46-47 1.2110289998875743 1106 48-49 1.3900790749186882 1106 50-51 0.732662299017349 1106 52-53 1.2454437504279596 1106 54-55 1.232292333183068 1106 56-57 1.310793555278778 1106 58-59 0.708818593102837 1106 60-61 1.877303772418827 1106 62-63 2.2838117629044525 1106 64-65 2.248533510987915 1106 66-67 2.4591624872300066 1106 68-69 1.9021267062484384 1106 70-71 2.313751635634816 1106 72-73 2.719268330668804 1106 74-75 2.9885918662926336 1106 76-77 3.0660801179319854 1106 78-79 3.4757098982381187 1106 80-81 3.8659690539589313 1106 82-83 3.9618031372906657 1106 84-85 4.190657547604207 1106 86-87 4.521896461925309 1106 88-89 4.597581236741778 1106 90-91 4.992627271096612 1106 92-93 5.3358356496237604 1106 94-95 5.424299503298041 1106 96-97 5.443202275594523 1106 98-99 5.555717891689266 1106 100-101 4.864467162431991 1107 1 0.5005449688590993 1107 2 0.3249205050487447 1107 3 0.3573822508363058 1107 4 0.3454450743003221 1107 5 0.309203134133476 1107 6 0.3613584482124139 1107 7 0.3894394642307333 1107 8 0.2830068019135936 1107 9 0.4646083823977847 1107 10-11 0.46162495825000605 1107 12-13 0.4593052775986095 1107 14-15 0.6343991157103233 1107 16-17 0.8358974627515181 1107 18-19 0.8297871502268208 1107 20-21 0.7961254918874658 1107 22-23 0.7315289482301353 1107 24-25 0.8575427573852465 1107 26-27 0.9110955462661892 1107 28-29 0.8976992476327652 1107 30-31 0.929855174744155 1107 32-33 0.9224288647190448 1107 34-35 0.9382492814649908 1107 36-37 1.007492152823545 1107 38-39 1.124200824197473 1107 40-41 1.0776311567670618 1107 42-43 1.2254665861569336 1107 44-45 1.3412217458997446 1107 46-47 1.243207476114236 1107 48-49 1.3538856379203352 1107 50-51 1.5308383700391985 1107 52-53 1.6303020804489492 1107 54-55 1.6593922349907189 1107 56-57 1.9635476747431966 1107 58-59 2.1978532215248414 1107 60-61 2.152968548509776 1107 62-63 2.376707341611475 1107 64-65 2.383404809402979 1107 66-67 2.51224082735337 1107 68-69 2.5977698986088456 1107 70-71 2.606000934422937 1107 72-73 2.893792223097705 1107 74-75 3.0650399009649334 1107 76-77 3.1796840065481753 1107 78-79 3.2799081132251295 1107 80-81 3.633438291307133 1107 82-83 3.8563985158305236 1107 84-85 4.046510713394532 1107 86-87 4.165404710796576 1107 88-89 4.424231254151845 1107 90-91 4.778540050433122 1107 92-93 4.998600288617169 1107 94-95 5.305915896904363 1107 96-97 5.4085368936634275 1107 98-99 5.4470121655164725 1107 100-101 4.938964804669878 1108 1 0.14259013613144234 1108 2 0.2570885332854971 1108 3 0.24494940484352412 1108 4 0.1978021554049363 1108 5 0.12678721531706572 1108 6 0.1040317303087619 1108 7 0.11301232275071271 1108 8 0.1134569578349982 1108 9 0.12121610271118755 1108 10-11 0.1749791465666206 1108 12-13 0.23743422499232736 1108 14-15 0.15747832411516072 1108 16-17 0.41322574342089524 1108 18-19 0.49142570173519573 1108 20-21 0.5041731712848261 1108 22-23 0.49516616846268846 1108 24-25 0.5314792096479692 1108 26-27 0.4770279637519508 1108 28-29 0.63007436322102 1108 30-31 0.5855421586188427 1108 32-33 0.612620483196956 1108 34-35 0.8308957374758492 1108 36-37 0.911897794157106 1108 38-39 0.9404391091999784 1108 40-41 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0.3682378289747277 1109 5 0.3616365440303184 1109 6 0.4456639366069979 1109 7 0.4741453999173899 1109 8 0.40077104675563646 1109 9 0.47515853149513987 1109 10-11 0.43824412531963475 1109 12-13 0.48919023085457525 1109 14-15 0.6268888400816977 1109 16-17 0.6442166922808497 1109 18-19 0.580953232593032 1109 20-21 0.6165500667585135 1109 22-23 0.6601989595131812 1109 24-25 0.5171134476657073 1109 26-27 0.4101120577910109 1109 28-29 0.5105221240028541 1109 30-31 0.45162162582411014 1109 32-33 0.6721039735198175 1109 34-35 0.5845825442793071 1109 36-37 0.7728464101575199 1109 38-39 0.8195498429756753 1109 40-41 0.7117029249811182 1109 42-43 0.8625391905250837 1109 44-45 0.8776586716734371 1109 46-47 1.2287233731161677 1109 48-49 1.2284902277011227 1109 50-51 1.4764065014866503 1109 52-53 1.6391761435533923 1109 54-55 1.675729980799872 1109 56-57 1.8199016902976695 1109 58-59 1.9395810886267135 1109 60-61 2.0293663362273833 1109 62-63 2.1608183989938077 1109 64-65 2.3991884771979493 1109 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1.1173302605878845 2311 54-55 1.2193555197504722 2311 56-57 1.3325817591737348 2311 58-59 1.2951047613466748 2311 60-61 1.3272422771072456 2311 62-63 1.4788631958368406 2311 64-65 1.5903668150377648 2311 66-67 1.5781090256529886 2311 68-69 1.5155529490627586 2311 70-71 1.6571999326835822 2311 72-73 1.6070642330653442 2311 74-75 1.754700134690296 2311 76-77 1.9887224652958757 2311 78-79 2.271385554394712 2311 80-81 2.2934450082680797 2311 82-83 2.5297012911378154 2311 84-85 2.867970574370027 2311 86-87 3.1830211847652414 2311 88-89 3.250976575601964 2311 90-91 3.3437093945550522 2311 92-93 3.4536807159319842 2311 94-95 3.5181653324930586 2311 96-97 3.5407162157893914 2311 98-99 3.9118027476738213 2311 100-101 3.5160104198833775 2312 1 -0.22839975447218208 2312 2 -0.16766767554436157 2312 3 -0.1624558473545541 2312 4 -0.13216342810284942 2312 5 -0.27520907236522163 2312 6 -0.11118096574404746 2312 7 -0.1998882374162676 2312 8 -0.24362772329150317 2312 9 -0.1872604311913193 2312 10-11 -0.20381474562782387 2312 12-13 -0.22215144527280728 2312 14-15 -0.10150367960617501 2312 16-17 0.004018322637534766 2312 18-19 -8.781747662069961E-4 2312 20-21 -0.01605130735050153 2312 22-23 -0.038312819077333415 2312 24-25 -0.1775868939080283 2312 26-27 0.01357906938458342 2312 28-29 -3.541433138494199E-4 2312 30-31 0.03731937079076886 2312 32-33 0.0010071248361782636 2312 34-35 0.02539706920170204 2312 36-37 0.21356146284412603 2312 38-39 0.17132593883188463 2312 40-41 0.1869388291141334 2312 42-43 0.23041642831357478 2312 44-45 0.34522123589253084 2312 46-47 0.26717231130056973 2312 48-49 0.4089767614507238 2312 50-51 0.5398953296094007 2312 52-53 0.6141036949825605 2312 54-55 0.7335755558796251 2312 56-57 0.8727302607895808 2312 58-59 1.070801752176127 2312 60-61 1.2066347298338513 2312 62-63 1.3009085392519069 2312 64-65 1.3952117209179526 2312 66-67 1.5062479368783386 2312 68-69 1.8138670572245559 2312 70-71 1.850506228448623 2312 72-73 1.7400154688675684 2312 74-75 1.7724159261008268 2312 76-77 1.8924838014037881 2312 78-79 2.3007619451995645 2312 80-81 2.2959767940996336 2312 82-83 2.5352741680414823 2312 84-85 2.8668217403333927 2312 86-87 3.036769762827703 2312 88-89 2.9955581167941823 2312 90-91 3.0803411993966066 2312 92-93 3.214052701907285 2312 94-95 3.1318857208662614 2312 96-97 3.27761843019978 2312 98-99 3.3500283424478035 2312 100-101 3.1975928343155235 2313 1 -0.4419179338161676 2313 2 -0.6044009708430416 2313 3 -0.4220677459216162 2313 4 -0.470305185371771 2313 5 -0.39549066077389483 2313 6 -0.41705286015569953 2313 7 -0.29234323824315567 2313 8 -0.24597309353244867 2313 9 -0.462682206889653 2313 10-11 -0.3572132278012319 2313 12-13 -0.4638516622223037 2313 14-15 -0.37100383788391866 2313 16-17 -0.3368196555427332 2313 18-19 -0.24166671989675592 2313 20-21 -0.20830422659005166 2313 22-23 -0.3750702709795988 2313 24-25 -0.37968987822843303 2313 26-27 -0.31455369514872444 2313 28-29 -0.2196034168659864 2313 30-31 -0.08696570472761067 2313 32-33 -0.03910418551358674 2313 34-35 -0.0035679583154291095 2313 36-37 0.11719826151023227 2313 38-39 0.168779396875685 2313 40-41 0.25251740876583284 2313 42-43 0.32914334730876504 2313 44-45 0.5439831399688231 2313 46-47 0.5402546159285464 2313 48-49 0.5538949359553911 2313 50-51 0.6099661776481682 2313 52-53 0.6489397470336442 2313 54-55 0.7524958456868802 2313 56-57 0.9513623551171264 2313 58-59 0.9200676953845139 2313 60-61 1.1174482194572164 2313 62-63 1.2050708140360733 2313 64-65 1.285962931297771 2313 66-67 1.2657593995666865 2313 68-69 1.33592285617922 2313 70-71 1.5197316853493774 2313 72-73 1.6088680082005382 2313 74-75 1.6385139527182844 2313 76-77 1.7931634658429694 2313 78-79 1.9177832919982087 2313 80-81 2.1685922043729526 2313 82-83 2.2231876474062844 2313 84-85 2.352029770084407 2313 86-87 2.3513326852808056 2313 88-89 2.2466014700987103 2313 90-91 2.3860115721421984 2313 92-93 2.511408943619699 2313 94-95 2.711187271068301 2313 96-97 2.9326775288905047 2313 98-99 2.971677038641115 2313 100-101 2.731529312846641 2314 1 -0.40943915137794207 2314 2 -0.46144645916442073 2314 3 -0.42574404125741694 2314 4 -0.32406373919269527 2314 5 -0.40999435189057465 2314 6 -0.290989278172205 2314 7 -0.2618621332595339 2314 8 -0.27500210692333127 2314 9 -0.4347013031959719 2314 10-11 -0.3900813710747997 2314 12-13 -0.3859514547634788 2314 14-15 -0.4781020020796305 2314 16-17 -0.40103184678299897 2314 18-19 -0.4040975853153128 2314 20-21 -0.39145303413243937 2314 22-23 -0.3406039256557989 2314 24-25 -0.3119276735488157 2314 26-27 -0.3997455054322572 2314 28-29 -0.44551912585321674 2314 30-31 -0.2878696868234272 2314 32-33 -0.11430630664886365 2314 34-35 -0.14415235608196753 2314 36-37 -0.034539301275209766 2314 38-39 0.01639850744254545 2314 40-41 0.2926377981747379 2314 42-43 0.42306132676637276 2314 44-45 0.3930127822655791 2314 46-47 0.4785184912145013 2314 48-49 0.6591845951805411 2314 50-51 0.5767288025330544 2314 52-53 0.7254900100324662 2314 54-55 0.6490694703358244 2314 56-57 0.7306065784964773 2314 58-59 1.1467658313796 2314 60-61 1.2308466585689075 2314 62-63 1.3119299339123422 2314 64-65 1.290961130704197 2314 66-67 1.4154867668665432 2314 68-69 1.71355109188994 2314 70-71 1.8892821858903588 2314 72-73 1.8885457560285097 2314 74-75 2.097466528007729 2314 76-77 2.1843601455404063 2314 78-79 2.2806883204011186 2314 80-81 2.4836112578175324 2314 82-83 2.65810890799003 2314 84-85 3.036593415559352 2314 86-87 3.1337424683323114 2314 88-89 3.304771358005251 2314 90-91 3.333396485371214 2314 92-93 3.596478978497938 2314 94-95 3.5174960782742026 2314 96-97 3.49499789514606 2314 98-99 3.561955475701861 2314 100-101 3.136964501692658 2315 1 -0.7091988358369186 2315 2 -0.574292036568103 2315 3 -0.5208418656328533 2315 4 -0.48677892698376013 2315 5 -0.41531991338078456 2315 6 -0.4192588980357854 2315 7 -0.44827526808560236 2315 8 -0.5608788563181548 2315 9 -0.7273399523150701 2315 10-11 -0.5665532297307863 2315 12-13 -0.6556817037852767 2315 14-15 -0.6645120734799121 2315 16-17 -0.5231472995399713 2315 18-19 -0.4359473594080754 2315 20-21 -0.4699472439513741 2315 22-23 -0.519376314958393 2315 24-25 -0.5458801728855818 2315 26-27 -0.5533218303792182 2315 28-29 -0.6228967373981646 2315 30-31 -0.5655001535322626 2315 32-33 -0.4227535092203496 2315 34-35 -0.5411685078922872 2315 36-37 -0.3588111402844163 2315 38-39 -0.3624484150092826 2315 40-41 -0.31458098087036745 2315 42-43 -0.2901188482124155 2315 44-45 -0.16088043365548543 2315 46-47 -0.0942764702261556 2315 48-49 -0.09494500488514035 2315 50-51 -0.026544743621229827 2315 52-53 0.08166222071691465 2315 54-55 0.0691799721882802 2315 56-57 0.24288514642253745 2315 58-59 0.5535468570921616 2315 60-61 0.4733292669901843 2315 62-63 0.5000665520822558 2315 64-65 0.6750989468778208 2315 66-67 0.9169751833388702 2315 68-69 0.856403997008087 2315 70-71 0.9489052844475054 2315 72-73 1.388621473702372 2315 74-75 1.4919649522836238 2315 76-77 1.395658304891235 2315 78-79 1.760720962223946 2315 80-81 1.9796322251289418 2315 82-83 2.029569604543614 2315 84-85 2.12129545349314 2315 86-87 2.2852939709528712 2315 88-89 2.635204410000366 2315 90-91 2.765875721981846 2315 92-93 2.8354351753896196 2315 94-95 2.7362369326718223 2315 96-97 2.7543697825778217 2315 98-99 2.773600414850989 2315 100-101 2.6123116337849215 2316 1 -0.9692669872653674 2316 2 -0.8535953832873311 2316 3 -0.8653415960735309 2316 4 -0.6221944596761091 2316 5 -0.5717122097029588 2316 6 -0.4698961596201059 2316 7 -0.5480908088641883 2316 8 -0.7360484159954126 2316 9 -0.5272060753299002 2316 10-11 -0.6204356352744824 2316 12-13 -0.6856430574000782 2316 14-15 -1.2658382707432807 2316 16-17 -0.9391072761158199 2316 18-19 -0.6697378283050881 2316 20-21 -0.48962539866295884 2316 22-23 -0.6059867462284387 2316 24-25 -0.8252350855947412 2316 26-27 -0.6624330867591652 2316 28-29 -0.6035779230440497 2316 30-31 -0.6020013070645334 2316 32-33 -0.728544802308356 2316 34-35 -0.5075345639361899 2316 36-37 -0.5164350746008068 2316 38-39 -0.5046344432637682 2316 40-41 -0.6215746155516015 2316 42-43 -0.6467497750716333 2316 44-45 -0.5443780520526786 2316 46-47 -0.53000351771216 2316 48-49 -0.3013389606160075 2316 50-51 -0.28828976266551365 2316 52-53 -0.04922604507992645 2316 54-55 -0.11433857529831926 2316 56-57 0.037243437947232394 2316 58-59 0.21200330647862486 2316 60-61 0.14733394338765393 2316 62-63 0.35404307366543364 2316 64-65 0.5182284489698112 2316 66-67 0.6559442505555175 2316 68-69 0.8724585869054948 2316 70-71 0.7302786218902639 2316 72-73 0.9313031948994421 2316 74-75 1.2173883798695684 2316 76-77 1.4128786429068398 2316 78-79 1.5320290400416745 2316 80-81 1.7880273844986583 2316 82-83 1.9442034054770545 2316 84-85 1.8946468201486226 2316 86-87 2.427193143118739 2316 88-89 2.2427371200806974 2316 90-91 2.5274915017369075 2316 92-93 2.568990192030732 2316 94-95 2.7856806632041753 2316 96-97 2.7253008340926783 2316 98-99 2.6303072312802804 2316 100-101 2.6675177826130163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9283.0 3 3459.0 4 3739.0 5 4308.0 6 5044.0 7 6491.0 8 8216.0 9 10493.0 10 13123.0 11 16353.0 12 19842.0 13 24087.0 14 27572.0 15 32314.0 16 37021.0 17 42387.0 18 42363.0 19 40713.0 20 43522.0 21 47662.0 22 53205.0 23 59721.0 24 66020.0 25 73657.0 26 82047.0 27 91546.0 28 102419.0 29 113523.0 30 126211.0 31 142235.0 32 161250.0 33 184811.0 34 214941.0 35 252603.0 36 290541.0 37 296856.0 38 162240.0 39 11459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34444630861545 18.711622237066972 14.491059012677852 23.452872441639723 2 15.305640573584245 20.101934943413582 40.148467485394846 24.443956997607327 3 19.68348237829003 23.871673609853907 29.225964383211917 27.21887962864415 4 12.849988158617037 16.36342231026066 36.89158905883593 33.89500047228638 5 13.975167822909352 37.214208162019105 33.87366628193701 14.936957733134534 6 32.31973170516319 35.69556285302177 17.44289527314498 14.541810168670047 7 29.126539500735017 32.13286038483754 20.341359222769416 18.39924089165802 8 26.437663848217817 34.701961781381954 19.717803793117252 19.142570577282978 9 26.49868212751366 16.904358253249168 20.107873218681963 36.48908640055521 10-11 25.22287182959957 26.451107126132666 27.079006427502943 21.24701461676482 12-13 26.27317014914341 23.69046321563182 27.407217173059156 22.62914946216561 14-15 22.855254542751364 25.277730206018127 26.049282365986077 25.817732885244425 16-17 22.40548171495712 28.42169227916194 26.585027622078776 22.587798383802163 18-19 22.428477747653623 27.164298659263046 28.659576131607682 21.747647461475648 20-21 23.225893771188346 26.108578447854143 28.690868330199514 21.974659450757994 22-23 22.661265334538065 26.18007569552816 28.555274827874882 22.603384142058893 24-25 22.576216702601567 26.275683300769735 28.744350285628133 22.403749711000565 26-27 22.326638346659138 26.495737026056975 28.775198175883503 22.402426451400387 28-29 22.1426359294982 27.032710000530425 28.659945691509293 22.16470837846208 30-31 22.344998926321715 26.481748752437163 28.558884195883405 22.614368125357714 32-33 22.203888357256233 26.584813297702066 28.506407280878896 22.704891064162805 34-35 22.851155048485534 26.527114241146688 28.336367118908594 22.28536359145918 36-37 22.748346648316435 26.18865609292646 28.517446466034556 22.545550792722548 38-39 22.286916093616966 26.67189463857453 28.47427901118112 22.566910256627388 40-41 22.654235230056337 26.546680413986618 28.31533915900821 22.483745196948835 42-43 22.368373782429902 26.53860589784814 28.161063280970133 22.931957038751825 44-45 22.461544251159552 26.562224972438443 28.163506531383398 22.812724245018607 46-47 22.758706225098493 26.90026396237809 27.76367583435548 22.57735397816793 48-49 22.658230771402007 26.76889074021645 27.793805826720874 22.779072661660667 50-51 22.551360349659536 27.01433628730322 27.82165475226063 22.61264861077661 52-53 22.84651728605262 27.098182534722113 27.40172629609902 22.653573883126242 54-55 22.450659399888714 26.87196306491675 27.70502428179644 22.972353253398094 56-57 22.63020559385656 26.927501203766184 27.593639325138312 22.84865387723894 58-59 22.898149765237577 26.664132793648033 27.528243931918638 22.909473509195752 60-61 22.506004377923183 26.826368323363525 27.986054414263332 22.681572884449956 62-63 22.426551899492857 26.933527371550177 27.87190330383248 22.768017425124494 64-65 22.204521837301094 26.996968051213848 27.7640810287244 23.034429082760653 66-67 22.32657736015197 26.855087471191585 27.548456464185882 23.26987870447056 68-69 22.49412173625078 26.687146367655508 27.713811259924114 23.1049206361696 70-71 22.51751481628153 26.767343538441935 27.534343238224597 23.18079840705194 72-73 22.60644057648719 26.653722790061156 27.64127785143053 23.098558782021126 74-75 22.327475386616786 27.15440979625909 27.456416072447293 23.06169874467683 76-77 22.535851406216725 26.748369185452287 27.438823606890516 23.276955801440472 78-79 22.66336788117804 26.891367919493582 27.388512043395085 23.0567521559333 80-81 22.70871955961053 26.97518926299217 27.28496853798893 23.031122639408373 82-83 22.66649195502498 26.9397995799736 27.32561351925982 23.068094945741603 84-85 22.731436193331554 26.818647457737864 27.33889022971588 23.111026119214703 86-87 22.706770106667584 26.957395397333883 27.211069359500183 23.124765136498347 88-89 22.68518336357616 26.92794394558652 27.0645113996958 23.322361291141522 90-91 22.745333538640526 26.805043713322725 27.083645571352804 23.36597717668395 92-93 22.992439826096327 26.642980003472598 27.20167505878187 23.162905111649202 94-95 22.795765773564415 26.566458830811257 27.23687573541764 23.40089966020668 96-97 22.78935735620661 26.562336413744497 27.237979724713203 23.41032650533569 98-99 22.909781009811 26.65305800570565 26.801541007224515 23.635619977258838 100-101 23.35592170915514 26.102673003466336 27.112386416016516 23.429018871362008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 612.0 1 445.5 2 367.5 3 466.0 4 413.5 5 406.0 6 668.0 7 1527.5 8 2490.0 9 2725.5 10 2344.5 11 2023.5 12 2121.0 13 2291.0 14 2484.0 15 2706.0 16 2889.0 17 3054.5 18 3303.0 19 3735.0 20 4168.5 21 4767.0 22 5661.5 23 6753.5 24 8157.5 25 10104.0 26 12944.0 27 16924.0 28 20759.0 29 25317.5 30 30815.5 31 35865.5 32 42258.5 33 47567.0 34 51848.0 35 57318.0 36 63987.5 37 70193.5 38 74376.5 39 78001.0 40 83007.5 41 89133.0 42 94037.5 43 99138.5 44 103881.0 45 108466.5 46 112089.0 47 117944.0 48 127062.5 49 126526.0 50 118015.5 51 110446.5 52 104106.0 53 100260.5 54 97174.5 55 91344.0 56 83037.0 57 75574.0 58 69405.0 59 61866.0 60 52294.0 61 43009.0 62 34931.5 63 26836.0 64 20327.0 65 16014.0 66 12337.0 67 9432.0 68 7127.5 69 5288.5 70 4104.0 71 3237.5 72 2659.5 73 2271.0 74 1810.0 75 1432.5 76 1050.0 77 680.5 78 472.0 79 327.5 80 214.5 81 125.0 82 58.0 83 39.0 84 34.0 85 22.5 86 19.5 87 18.5 88 10.0 89 6.5 90 5.0 91 2.5 92 3.5 93 4.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2964481299582626 2 0.03492655673752436 3 0.042007651002624796 4 0.045189012194191656 5 0.0596932825729481 6 0.05866703702728137 7 0.030821574554857442 8 0.03366085389786873 9 0.03909995528990239 10-11 0.031249176865551915 12-13 0.03268592062948534 14-15 0.02707577831317388 16-17 0.021038033686167957 18-19 0.025399577255251552 20-21 0.03839868750036346 22-23 0.031180760495840797 24-25 0.0533134560973866 26-27 0.04496665899263053 28-29 0.03701325601371338 30-31 0.036927735551574484 32-33 0.032070173302085295 34-35 0.03114655231098524 36-37 0.03010320267289073 38-39 0.018386899359862235 40-41 0.017976401141595547 42-43 0.018814501670556708 44-45 0.009390146742850575 46-47 0.01751459064604552 48-49 0.015376579092573164 50-51 0.007012677895389318 52-53 0.0047891458797780714 54-55 0.006927157433250424 56-57 0.005456205484461445 58-59 0.007371863836372674 60-61 0.006892949248394866 62-63 0.004840458157061407 64-65 0.008192860272906057 66-67 0.0029419038975779578 68-69 0.007525800668222682 70-71 0.013495128925517493 72-73 0.013751690311934175 74-75 0.011100555985628457 76-77 0.009390146742850575 78-79 0.006191681458855935 80-81 0.007799466147067144 82-83 0.01250309156470632 84-85 0.004190502644805812 86-87 0.0012485987472278543 88-89 0.0186605648387067 90-91 0.028290168875546175 92-93 0.013495128925517493 94-95 0.02114065824073463 96-97 0.01734354972176773 98-99 0.013118838892106358 100-101 0.10915831787408445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2923277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.7040891215885 #Duplication Level Percentage of deduplicated Percentage of total 1 75.217606734781 40.39493055597275 2 13.44927885315662 14.44562540302038 3 4.735577636303905 7.62959650266799 4 2.2132186642053506 4.754355695521889 5 1.2074132938895925 3.24215155708187 6 0.7201314075620245 2.3204400774579566 7 0.47317228738321543 1.7787900684045863 8 0.33931303061134116 1.4577997788854202 9 0.24854345367012043 1.2013019807838796 >10 1.2621131368317215 12.1414507076898 >50 0.08404135053346931 3.113481929622802 >100 0.04484318218476609 4.23941558609642 >500 0.002822522040891995 1.0485613961560034 >1k 0.0017320021613851787 1.4780523093349398 >5k 1.9244468459835322E-4 0.7540464513034486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9165 0.31351801420118586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6738 0.23049474955674745 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5985 0.20473598636051252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4966 0.16987784599269928 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3187 0.10902148513466223 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.131227728333647E-5 0.0 16-17 0.0 0.0 0.0 6.84163697111153E-5 0.0 18-19 0.0 0.0 0.0 6.84163697111153E-5 0.0 20-21 0.0 0.0 0.0 6.84163697111153E-5 0.0 22-23 0.0 0.0 0.0 2.736654788444612E-4 0.0 24-25 0.0 0.0 0.0 5.986432349722589E-4 0.0 26-27 0.0 0.0 0.0 0.0015222642260723156 0.0 28-29 0.0 0.0 0.0 0.0042589190145169275 0.0 30-31 0.0 0.0 0.0 0.014093772160489751 0.0 32-33 0.0 0.0 0.0 0.032070173302085295 0.0 34-35 0.0 0.0 0.0 0.06278912330237607 0.0 36-37 0.0 0.0 0.0 0.1109884557638568 0.0 38-39 0.0 0.0 0.0 0.1843308040941724 0.0 40-41 0.0 0.0 0.0 0.26432664437889397 0.0 42-43 0.0 0.0 0.0 0.3498128983329325 0.0 44-45 0.0 0.0 0.0 0.45440442352880006 0.0 46-47 0.0 0.0 0.0 0.5801708151502578 0.0 48-49 0.0 0.0 0.0 0.7102474380635158 0.0 50-51 0.0 0.0 0.0 0.8501589141227466 0.0 52-53 0.0 0.0 0.0 0.9879665868133605 0.0 54-55 0.0 0.0 0.0 1.1412705672435421 0.0 56-57 0.0 0.0 0.0 1.3238909620949366 0.0 58-59 0.0 0.0 0.0 1.5185697421079152 0.0 60-61 0.0 0.0 0.0 1.7294803058348558 0.0 62-63 0.0 0.0 0.0 1.9427170261319744 0.0 64-65 0.0 0.0 0.0 2.164540000827838 0.0 66-67 0.0 0.0 0.0 2.4193054575396036 0.0 68-69 0.0 0.0 0.0 2.7038320350757044 0.0 70-71 0.0 0.0 0.0 3.006300805568545 0.0 72-73 0.0 0.0 0.0 3.309299802926647 0.0 74-75 0.0 0.0 0.0 3.6231256907915332 0.0 76-77 0.0 0.0 0.0 3.9634116096421925 0.0 78-79 0.0 0.0 0.0 4.345842012234899 0.0 80-81 0.0 0.0 0.0 4.751585292806668 3.420818485555765E-5 82-83 0.0 0.0 0.0 5.161621700577811 3.420818485555765E-5 84-85 0.0 0.0 0.0 5.571794941088374 3.420818485555765E-5 86-87 0.0 0.0 0.0 5.998285485775039 3.420818485555765E-5 88-89 0.0 0.0 0.0 6.4593091930733895 3.420818485555765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11995 0.0 27.015123 1 TACATGG 11935 0.0 26.481552 2 GTATCAA 16340 0.0 25.401796 1 ACATGGG 12715 0.0 24.296362 3 TATCAAC 18940 0.0 21.756216 2 GAGTACT 8315 0.0 21.651638 12-13 ATCAACG 19175 0.0 21.539154 3 TCAACGC 19315 0.0 21.33382 4 CAACGCA 19360 0.0 21.284231 5 GGTATCA 12055 0.0 21.148758 1 AGTACTT 8740 0.0 21.06076 12-13 AACGCAG 19825 0.0 20.829742 6 AGAGTAC 14735 0.0 20.599697 10-11 GTACTTT 8875 0.0 20.09932 14-15 CATGGGG 6420 0.0 20.062197 4 ACCGTAC 145 6.145099E-4 19.657095 8 ACGCAGA 21860 0.0 18.862778 7 CGCAGAG 22135 0.0 18.628431 8 CATGGGA 6565 0.0 18.38837 4 GTGTAGC 1720 0.0 18.285793 1 >>END_MODULE