FastQCFastQC Report
Thu 26 May 2016
SRR938341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923277
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.30845520284256334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72320.24739359287539292No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62850.2149984418171798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52040.178019393988322No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33270.1138106310144403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169900.025.033371
TACATGG118850.023.5771472
GTACATG122000.023.5143321
ACCGTGC4300.023.1943668
GGTATCA126750.022.2202471
ACATGGG124550.021.8008843
GAGTACT93150.021.61795812-13
ATCAACG198800.021.1215633
TATCAAC200600.021.0599422
TCAACGC200600.020.9557424
CAACGCA202900.020.7416325
GTACTTT96850.020.64496414-15
AGTACTT97300.020.622712-13
AACGCAG206350.020.3690366
GCACCGT5700.019.9971476
AGAGTAC160050.019.73320610-11
TGCACCG4650.019.4304285
CATGGGG60700.019.0370354
CCGTGCG2854.285903E-818.3307179
TACGGTA1300.007516968518.2897764
Produced by FastQC (version 0.11.5)