##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938341_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2923277 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799376179540975 31.0 31.0 34.0 30.0 34.0 2 32.02651134326306 31.0 31.0 34.0 30.0 34.0 3 32.099284467397375 33.0 31.0 34.0 30.0 34.0 4 35.76197055564697 37.0 35.0 37.0 35.0 37.0 5 35.632750505682495 37.0 35.0 37.0 33.0 37.0 6 35.672736110878304 37.0 35.0 37.0 33.0 37.0 7 35.6126774848911 37.0 35.0 37.0 33.0 37.0 8 35.60181843869055 37.0 35.0 37.0 33.0 37.0 9 37.16727699769813 39.0 37.0 39.0 33.0 39.0 10-11 37.09753420561924 39.0 37.0 39.0 33.0 39.0 12-13 36.99192806566056 39.0 37.0 39.0 33.0 39.0 14-15 38.0286857865334 40.0 37.0 41.0 33.0 41.0 16-17 37.95694455229525 40.0 37.0 41.0 33.0 41.0 18-19 37.95453219109923 40.0 37.0 41.0 33.0 41.0 20-21 37.95035639797392 40.0 37.0 41.0 32.5 41.0 22-23 37.98015035865571 40.0 37.0 41.0 33.0 41.0 24-25 37.91125336394738 40.0 37.0 41.0 32.5 41.0 26-27 37.736326560910925 40.0 37.0 41.0 32.0 41.0 28-29 37.606988492708695 39.5 37.0 41.0 32.0 41.0 30-31 37.38285065014366 39.0 36.0 41.0 31.5 41.0 32-33 37.16701701549323 39.0 36.0 41.0 31.0 41.0 34-35 36.9909279893763 39.0 36.0 40.5 31.0 41.0 36-37 37.00646089987367 39.0 36.0 41.0 31.0 41.0 38-39 36.8603479930229 39.0 35.0 41.0 30.0 41.0 40-41 36.695606163904415 39.0 35.0 41.0 30.0 41.0 42-43 36.47506993008189 39.0 35.0 40.0 30.0 41.0 44-45 36.194774562930576 39.0 35.0 40.0 30.0 41.0 46-47 35.836259273411315 38.0 35.0 40.0 28.5 41.0 48-49 35.44363329236333 38.0 34.5 40.0 27.5 41.0 50-51 35.079117374097635 38.0 34.0 40.0 26.5 41.0 52-53 34.58129455402276 38.0 33.0 40.0 25.0 41.0 54-55 34.26092806121349 38.0 33.0 40.0 24.0 41.0 56-57 33.89053295325759 37.0 33.0 40.0 23.0 41.0 58-59 33.43797115360604 37.0 32.0 39.5 22.5 41.0 60-61 33.014641787281874 36.0 31.0 39.0 22.0 40.5 62-63 32.543083327375406 36.0 31.0 39.0 20.0 40.0 64-65 32.039441524015686 35.0 31.0 38.5 18.5 40.0 66-67 31.53347202471747 35.0 30.0 38.0 16.5 40.0 68-69 30.957480936633786 34.5 29.0 38.0 12.5 40.0 70-71 30.37721827250719 34.0 29.0 37.0 8.0 39.0 72-73 29.778613179661043 34.0 28.0 36.0 7.0 39.0 74-75 29.15465930871416 33.5 27.5 36.0 4.5 38.5 76-77 27.856844390729993 31.5 26.0 34.5 2.0 36.5 78-79 28.47183708557212 33.0 27.0 35.0 2.0 37.0 80-81 28.144353750944575 33.0 26.0 35.0 2.0 37.0 82-83 27.62639342764986 33.0 26.0 35.0 2.0 36.0 84-85 27.016767312847875 32.0 25.5 35.0 2.0 36.0 86-87 26.357028943887286 32.0 24.5 34.5 2.0 35.0 88-89 25.58765265830094 31.5 21.5 34.0 2.0 35.0 90-91 25.01525171921785 31.0 19.5 34.0 2.0 35.0 92-93 24.21415042091461 31.0 15.0 34.0 2.0 35.0 94-95 23.40392562866947 30.5 4.5 34.0 2.0 35.0 96-97 22.391784801782386 30.0 2.0 34.0 2.0 35.0 98-99 21.18875563280524 29.5 2.0 34.0 2.0 35.0 100-101 18.05119767986407 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.269587121216901 1101 2 0.2834955022322134 1101 3 0.20738008558485888 1101 4 0.1546617608423233 1101 5 0.11415136545043936 1101 6 0.24311706870673788 1101 7 0.21358817325285884 1101 8 0.28449729010290525 1101 9 0.3460965700792613 1101 10-11 0.32584489577809705 1101 12-13 0.31043864734202486 1101 14-15 0.5247032455165126 1101 16-17 0.4628858160682867 1101 18-19 0.6180582829483754 1101 20-21 0.5655840475987617 1101 22-23 0.5263945116261226 1101 24-25 0.39868456274828645 1101 26-27 0.5173543507041813 1101 28-29 0.4215156531882016 1101 30-31 0.5549814726919848 1101 32-33 0.5669538123679345 1101 34-35 0.41163267192874287 1101 36-37 0.5591783449960275 1101 38-39 0.7217768243735136 1101 40-41 0.655661596447807 1101 42-43 0.5444423348052041 1101 44-45 0.6163798125047464 1101 46-47 0.6724374916429667 1101 48-49 0.8474131483261402 1101 50-51 0.7818247425632237 1101 52-53 0.9552087613593443 1101 54-55 1.0798824557386908 1101 56-57 1.0991183498797241 1101 58-59 1.262645750871819 1101 60-61 1.3326806314419883 1101 62-63 1.33091182510762 1101 64-65 1.4721238987106275 1101 66-67 1.3451909735120289 1101 68-69 1.525915504331813 1101 70-71 1.7877449056210502 1101 72-73 1.8444079054678006 1101 74-75 1.8208459115810882 1101 76-77 1.676493838051119 1101 78-79 1.954462379406781 1101 80-81 2.261994932303004 1101 82-83 2.435354788697648 1101 84-85 2.4593448651742413 1101 86-87 2.576805389429076 1101 88-89 2.8005827516529855 1101 90-91 2.9873778978021797 1101 92-93 3.4835783028120666 1101 94-95 3.5827012836935452 1101 96-97 3.83022150613132 1101 98-99 4.249118050140293 1101 100-101 4.9230519295796 1102 1 0.4017152058688396 1102 2 0.3580027943408055 1102 3 0.3387702135537438 1102 4 0.22767367615222156 1102 5 0.14102731278213554 1102 6 0.16794674777000296 1102 7 0.22742234862347033 1102 8 0.32734978972285944 1102 9 0.47142504730736334 1102 10-11 0.37023405248353214 1102 12-13 0.39764830985500055 1102 14-15 0.6572850316871737 1102 16-17 0.5260886701962235 1102 18-19 0.6410970081825909 1102 20-21 0.6102348157364901 1102 22-23 0.5550537164622114 1102 24-25 0.49330559508620553 1102 26-27 0.5321349195834273 1102 28-29 0.5272773380854048 1102 30-31 0.5522814620697787 1102 32-33 0.4114651561780178 1102 34-35 0.5479562712135575 1102 36-37 0.6852578817408954 1102 38-39 0.8249006416996565 1102 40-41 0.6512632423163822 1102 42-43 0.6354787880334953 1102 44-45 0.5666552582396136 1102 46-47 0.5833003768487828 1102 48-49 0.893761814731171 1102 50-51 0.8341030772821014 1102 52-53 0.9753854715176615 1102 54-55 0.9923149459322431 1102 56-57 1.1194796446177904 1102 58-59 1.2559418704612568 1102 60-61 1.3610951440388348 1102 62-63 1.5342817976107526 1102 64-65 1.3769647597067447 1102 66-67 1.3860399480880794 1102 68-69 1.6130562815588334 1102 70-71 1.8026227916632855 1102 72-73 1.8349157293224643 1102 74-75 1.903998466093011 1102 76-77 1.915660879463072 1102 78-79 2.1941241482002845 1102 80-81 2.3914662584051847 1102 82-83 2.431123654710998 1102 84-85 2.6209185791088494 1102 86-87 2.756437988516076 1102 88-89 2.9018530268570224 1102 90-91 3.1050188916075925 1102 92-93 3.2478683741080197 1102 94-95 3.4419470184349485 1102 96-97 3.8686133813567274 1102 98-99 4.248266747585049 1102 100-101 4.7996173206705635 1103 1 0.34169161132899717 1103 2 0.26909912591086993 1103 3 0.2648190894253304 1103 4 0.21354691628475564 1103 5 0.1435740114300117 1103 6 0.20102814673101932 1103 7 0.2568404298781175 1103 8 0.25583431384745126 1103 9 0.28890479528656243 1103 10-11 0.30502056421451584 1103 12-13 0.41553482271116593 1103 14-15 0.5659051106748265 1103 16-17 0.6177934258437716 1103 18-19 0.4825130083430551 1103 20-21 0.5335932878787375 1103 22-23 0.49605978411881324 1103 24-25 0.4765207804205076 1103 26-27 0.48215967354809663 1103 28-29 0.5364011431317905 1103 30-31 0.5985714371543764 1103 32-33 0.7169216197757464 1103 34-35 0.7694147909558211 1103 36-37 0.8954150058194017 1103 38-39 0.8405207725434352 1103 40-41 0.7244581719608121 1103 42-43 0.9283919383081596 1103 44-45 0.9686442344087851 1103 46-47 1.065759918058994 1103 48-49 1.1678028798952553 1103 50-51 1.2513699215967833 1103 52-53 1.3061289539112835 1103 54-55 1.339145937015438 1103 56-57 1.3287217648095364 1103 58-59 1.6280612868156012 1103 60-61 1.5910717060458026 1103 62-63 1.7635063845595411 1103 64-65 1.6989361781725094 1103 66-67 1.5924821429330969 1103 68-69 1.6638732113138275 1103 70-71 1.8005099600592267 1103 72-73 1.8950994174381641 1103 74-75 2.170562402990946 1103 76-77 1.932140305542891 1103 78-79 2.1341224457537002 1103 80-81 2.4180706553159297 1103 82-83 2.6207274053769325 1103 84-85 2.753478016753565 1103 86-87 2.933988613064475 1103 88-89 3.11745877496244 1103 90-91 3.199709043883608 1103 92-93 3.293255697827746 1103 94-95 3.2767664661475777 1103 96-97 3.6309728133266184 1103 98-99 3.8818538294399545 1103 100-101 4.481630752387048 1104 1 0.26290992725141393 1104 2 0.30352963105578823 1104 3 0.28047095145518597 1104 4 0.1651534026764523 1104 5 0.1691463140449585 1104 6 0.15978415986126038 1104 7 0.22796387896391224 1104 8 0.3048073360324892 1104 9 0.313090941370298 1104 10-11 0.23122825076031006 1104 12-13 0.38607771380833356 1104 14-15 0.4624249342990012 1104 16-17 0.4481641527550764 1104 18-19 0.3756693501957429 1104 20-21 0.45527451105460415 1104 22-23 0.3887513530968931 1104 24-25 0.49283342214942394 1104 26-27 0.533889850623531 1104 28-29 0.5006363690445212 1104 30-31 0.5395288412917623 1104 32-33 0.4828733804090888 1104 34-35 0.48404902659682847 1104 36-37 0.6764906576907634 1104 38-39 0.6526689280504243 1104 40-41 0.6759436261789418 1104 42-43 0.6329701260250928 1104 44-45 0.5628315185214348 1104 46-47 0.5288926784600676 1104 48-49 0.682044565521295 1104 50-51 0.7102870777558081 1104 52-53 0.8875233238397087 1104 54-55 0.8099332244862083 1104 56-57 0.940730045369115 1104 58-59 1.3026966045168962 1104 60-61 1.2111535788309595 1104 62-63 1.1936749014560064 1104 64-65 1.2978189975738488 1104 66-67 1.4683637655384523 1104 68-69 1.5874356802395795 1104 70-71 1.6989692134936014 1104 72-73 1.8525292071173602 1104 74-75 1.960353420771252 1104 76-77 1.7537030850945605 1104 78-79 2.050580862456979 1104 80-81 2.3016747835624614 1104 82-83 2.4858637496362768 1104 84-85 2.438810689657007 1104 86-87 2.6268660911923973 1104 88-89 2.6479093271396614 1104 90-91 2.942785024104296 1104 92-93 3.4027137908457554 1104 94-95 3.384133081229816 1104 96-97 3.6280625608987442 1104 98-99 4.012161288473244 1104 100-101 4.276685970109703 1105 1 0.19295023510514042 1105 2 0.20787318412336475 1105 3 0.20338865570045783 1105 4 0.22935843484968643 1105 5 0.0946591268956638 1105 6 0.15743142961841983 1105 7 0.2128248600318443 1105 8 0.171955439172784 1105 9 0.2032424112456539 1105 10-11 0.2657326368125936 1105 12-13 0.3039197495209578 1105 14-15 0.3294800703961087 1105 16-17 0.3926595077231738 1105 18-19 0.3709662138861347 1105 20-21 0.36241770022261477 1105 22-23 0.29544630830669405 1105 24-25 0.2701325303298532 1105 26-27 0.39666850273421517 1105 28-29 0.3858121099586498 1105 30-31 0.4437236470902519 1105 32-33 0.4343339590625277 1105 34-35 0.5033523050132018 1105 36-37 0.6134759356954262 1105 38-39 0.5905991496440137 1105 40-41 0.5155807890904001 1105 42-43 0.5843357454844238 1105 44-45 0.5719957448032247 1105 46-47 0.7827956341479876 1105 48-49 0.8000781797479064 1105 50-51 0.8085266803881055 1105 52-53 0.9382248929905899 1105 54-55 0.9430587100729895 1105 56-57 0.9182987579747817 1105 58-59 1.0620653582936868 1105 60-61 1.3407446566510188 1105 62-63 1.2971804230343196 1105 64-65 1.4502680151114262 1105 66-67 1.4472469657296934 1105 68-69 1.4000795301376456 1105 70-71 1.4165061180894902 1105 72-73 1.5604383783161673 1105 74-75 1.6720231088668207 1105 76-77 1.635486192982814 1105 78-79 1.918425222345686 1105 80-81 2.212780028895846 1105 82-83 2.2585281186888757 1105 84-85 2.366308680904819 1105 86-87 2.51890279989345 1105 88-89 2.616913118866922 1105 90-91 2.808879279405076 1105 92-93 3.077909851699321 1105 94-95 3.1902410159669543 1105 96-97 3.4809651247969846 1105 98-99 3.915575835159302 1105 100-101 4.312213657687309 1106 1 0.1861207757596084 1106 2 0.21223563847138394 1106 3 0.16971444684477888 1106 4 0.15445067924006395 1106 5 0.07132325995328159 1106 6 0.17358368513897204 1106 7 0.188127925034955 1106 8 0.13889814752340612 1106 9 0.19023614581857373 1106 10-11 0.21301426239386245 1106 12-13 0.19369282002277544 1106 14-15 0.11428671398370227 1106 16-17 0.2004606448600157 1106 18-19 0.24548894943156796 1106 20-21 0.42161602870447723 1106 22-23 0.34873491071705587 1106 24-25 0.21143826834583024 1106 26-27 0.2290738049473049 1106 28-29 0.2801863361844923 1106 30-31 0.3077411433755799 1106 32-33 0.2513455948556711 1106 34-35 0.3117496700598039 1106 36-37 0.3780743918534739 1106 38-39 0.39822310010476514 1106 40-41 0.3782993405298569 1106 42-43 0.5224803913268161 1106 44-45 0.07330243430555328 1106 46-47 -0.018330992091577514 1106 48-49 0.4149870859922018 1106 50-51 0.2701936734367294 1106 52-53 0.44335097046591443 1106 54-55 0.461097951140907 1106 56-57 0.501795061007698 1106 58-59 0.8232288380342183 1106 60-61 0.6281007305084856 1106 62-63 0.8721000169045325 1106 64-65 0.5784608327170204 1106 66-67 0.6177101736327906 1106 68-69 0.40555649586975306 1106 70-71 0.636253664416369 1106 72-73 0.9763521537366238 1106 74-75 1.2132438216186188 1106 76-77 1.2233212185235836 1106 78-79 1.6067188975060311 1106 80-81 1.57825700569175 1106 82-83 1.808484803931652 1106 84-85 1.9066410262412639 1106 86-87 2.18385479961907 1106 88-89 2.4721605108538576 1106 90-91 2.4872052497828747 1106 92-93 2.1973371011624536 1106 94-95 2.7145646032033177 1106 96-97 3.289461842722215 1106 98-99 3.5036990725584687 1106 100-101 3.8452840599337037 1107 1 0.1318832773455334 1107 2 0.1215188040045021 1107 3 0.08433994231847919 1107 4 0.07451933391831744 1107 5 0.04543888572261068 1107 6 0.009454717053543504 1107 7 0.17358668089477902 1107 8 0.12916158531930932 1107 9 0.07869293502143648 1107 10-11 0.20451023797696877 1107 12-13 0.22453787516330692 1107 14-15 0.27752572602550885 1107 16-17 0.3247122874692323 1107 18-19 0.2954384483436243 1107 20-21 0.20305325313960765 1107 22-23 0.14268890490678388 1107 24-25 0.3159445583411653 1107 26-27 0.27165525672876356 1107 28-29 0.2536042083174195 1107 30-31 0.2875222822729171 1107 32-33 0.33516670191127673 1107 34-35 0.36855010138858546 1107 36-37 0.3668238236757162 1107 38-39 0.42445210940807243 1107 40-41 0.5148383744233982 1107 42-43 0.3557601219646287 1107 44-45 0.33174480481560664 1107 46-47 0.3874725912053947 1107 48-49 0.47978944874714813 1107 50-51 0.5385156367305441 1107 52-53 0.6876730292935775 1107 54-55 0.5379878838414314 1107 56-57 0.5062790453652255 1107 58-59 0.5754942543747816 1107 60-61 0.7372343056159139 1107 62-63 0.9491727499265821 1107 64-65 0.8789122625148877 1107 66-67 1.14955017477401 1107 68-69 1.138862876557397 1107 70-71 1.218583027908135 1107 72-73 1.4315627247933698 1107 74-75 1.3702979363495693 1107 76-77 1.418921345008659 1107 78-79 1.5653039527719201 1107 80-81 1.8601442334014031 1107 82-83 1.9141141028192017 1107 84-85 2.079380413610007 1107 86-87 2.3108370997064114 1107 88-89 2.3310020809546224 1107 90-91 2.5290725753304173 1107 92-93 2.868980767301519 1107 94-95 2.9847996207636065 1107 96-97 3.3002053466915164 1107 98-99 3.5596677975470286 1107 100-101 3.824678308407705 1108 1 0.14221905051552142 1108 2 0.10802804082744188 1108 3 0.03348069277195975 1108 4 0.08954507567838732 1108 5 -0.07642780033238239 1108 6 -0.04564217651083169 1108 7 0.0978965882027154 1108 8 0.09309658763630324 1108 9 0.14280936858947513 1108 10-11 0.11617250257493339 1108 12-13 0.1864826493624605 1108 14-15 0.23652851017204313 1108 16-17 0.19604015362962457 1108 18-19 0.2814936891871298 1108 20-21 0.3015355945136662 1108 22-23 0.2962753477887432 1108 24-25 0.19397361826236903 1108 26-27 0.22987446428953717 1108 28-29 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1.1054868236191275 2316 78-79 1.101355169715216 2316 80-81 1.2916328018700938 2316 82-83 1.4402313677935368 2316 84-85 1.7725478314708987 2316 86-87 1.8513036765885644 2316 88-89 2.037698559797878 2316 90-91 2.3061794368428785 2316 92-93 2.2249938225838406 2316 94-95 2.9770520319663056 2316 96-97 3.166934186000791 2316 98-99 3.7195891161387564 2316 100-101 4.06201218598131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 42.0 5 98.0 6 241.0 7 504.0 8 1200.0 9 2205.0 10 3419.0 11 5573.0 12 7888.0 13 11512.0 14 14756.0 15 19804.0 16 24668.0 17 30693.0 18 29980.0 19 27195.0 20 27008.0 21 29614.0 22 32459.0 23 36617.0 24 42371.0 25 49756.0 26 58352.0 27 69235.0 28 82301.0 29 97743.0 30 114955.0 31 135347.0 32 161336.0 33 195265.0 34 240979.0 35 303113.0 36 382454.0 37 431645.0 38 239830.0 39 13093.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.207270199597986 19.51950482951319 15.080409786828202 23.19281518406062 2 15.75315344729105 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28.283837279470823 22.745908929815176 46-47 22.69512013234467 26.932421023536605 27.856640974030505 22.515817870088217 48-49 22.59226546098779 26.75853160682344 27.86600448742969 22.783198444759083 50-51 22.457758878135735 27.04458044858561 27.920224460425747 22.577436212852906 52-53 22.809367706173585 27.093019238341082 27.434724796863247 22.66288825862209 54-55 22.433949981476268 26.84740447107818 27.696468723285545 23.02217682416001 56-57 22.59347985155016 26.93001723750435 27.602618568134325 22.873884342811166 58-59 22.875355294759956 26.70554312848218 27.498437541156722 22.920664035601142 60-61 22.464241329165866 26.912331605934025 27.914939295865565 22.708487769034548 62-63 22.38802549330768 27.00354773085137 27.786025751237396 22.822401024603554 64-65 22.148893177074903 27.082038411002447 27.650373194192685 23.118695217729964 66-67 22.253123684585464 26.91649643780827 27.515226524538182 23.31515335306808 68-69 22.443817635076876 26.749679853438124 27.603415421186643 23.20308709029835 70-71 22.420283811626472 26.827649244324093 27.438453489012502 23.313613455036933 72-73 22.49469430871392 26.724503604859752 27.558037626279557 23.222764460146767 74-75 22.21094682440289 27.22992039413302 27.22593514059735 23.33319764086674 76-77 22.271119021563813 26.778646019518504 27.492810294747983 23.457424664169697 78-79 22.384775715746404 26.93533661024939 27.49570088636828 23.18418678763593 80-81 22.320644989256053 27.050633476477653 27.390338262909715 23.23838327135658 82-83 22.319199819652226 26.981713105419637 27.4600349406703 23.239052134257832 84-85 22.295877821752576 26.84957848395167 27.458632654827568 23.39591103946819 86-87 22.26183210911758 27.006118570130592 27.28168684524526 23.450362475506562 88-89 22.123877458029963 27.063592353236576 27.0483354975719 23.76419469116156 90-91 22.152482444185374 26.841872465148352 27.27939948988249 23.726245600783784 92-93 22.288255568548994 26.72607615340421 27.37842768912422 23.60724058892258 94-95 22.00439780424503 26.648449667958253 27.379068080103252 23.96808444769346 96-97 21.89944709310818 26.656762256878153 27.46494772818313 23.978842921830534 98-99 21.97258419232936 26.737254115843278 27.076411164593704 24.21375052723365 100-101 21.939603233042646 26.437743575007634 27.389902172323467 24.232751019626257 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 646.0 1 434.5 2 311.5 3 432.0 4 394.5 5 379.5 6 688.5 7 1570.5 8 2420.0 9 2676.5 10 2367.0 11 2018.5 12 2056.5 13 2205.5 14 2510.0 15 2720.5 16 2724.5 17 2900.0 18 3322.5 19 3608.0 20 3896.5 21 4545.0 22 5456.0 23 6592.0 24 8001.5 25 9954.5 26 12615.5 27 16497.5 28 20410.0 29 25202.5 30 31253.0 31 36268.5 32 42939.5 33 48261.5 34 51678.5 35 56991.5 36 63714.5 37 70359.5 38 75083.0 39 78982.0 40 83785.5 41 89749.0 42 95073.0 43 100145.0 44 105343.5 45 110272.0 46 113582.5 47 120398.5 48 131096.0 49 130015.5 50 120095.5 51 112100.5 52 105318.0 53 100787.0 54 98113.5 55 92118.5 56 82408.0 57 74542.0 58 68427.5 59 60384.0 60 50037.5 61 40609.0 62 32780.0 63 24564.0 64 18106.0 65 14071.0 66 10569.0 67 8099.5 68 6029.5 69 4317.5 70 3400.0 71 2719.0 72 2243.5 73 1956.5 74 1636.0 75 1311.0 76 878.5 77 537.5 78 359.5 79 228.5 80 116.5 81 68.0 82 46.5 83 25.5 84 13.0 85 9.0 86 7.5 87 6.5 88 4.5 89 2.0 90 1.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.4208184855557655E-5 2 0.045017971269913866 3 1.0262455456667295E-4 4 0.0 5 0.10690057767361764 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011459741926611814 26-27 0.012314946548000753 28-29 0.0 30-31 0.0 32-33 4.447064031222494E-4 34-35 0.015462099554712058 36-37 0.003574755317405774 38-39 0.012349154732856313 40-41 0.007235031096950443 42-43 0.00596932825729481 44-45 0.0027708629733001694 46-47 3.4208184855557655E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.223532015611247E-4 68-69 2.223532015611247E-4 70-71 0.0 72-73 3.4208184855557655E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1972864699445176E-4 82-83 0.0012314946548000754 84-85 0.004720729510066956 86-87 0.001539368318500094 88-89 3.4208184855557655E-5 90-91 9.920373608111718E-4 92-93 2.223532015611247E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.381005289611623E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2923277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.14606719399384 #Duplication Level Percentage of deduplicated Percentage of total 1 73.10148374864698 37.38853399788947 2 14.465379277551554 14.796945210325156 3 5.142234710936724 7.890152461585714 4 2.4277605012103036 4.966816069033053 5 1.3352092933006388 3.4145352116599748 6 0.7927938985671706 2.4328974004262904 7 0.5233297453701076 1.8736380824920642 8 0.38082758095604924 1.5582266435923375 9 0.2636280857492472 1.213518580915947 >10 1.4146921525903982 12.896269687462864 >50 0.09713998487527205 3.424145128768663 >100 0.050022537005758934 4.584174462587413 >500 0.0034197883565950282 1.195621171263976 >1k 0.001810476188612109 1.4132282026473555 >5k 2.6821869460920135E-4 0.951297689349682 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9017 0.30845520284256334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7232 0.24739359287539292 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6285 0.2149984418171798 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5204 0.178019393988322 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3327 0.1138106310144403 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.420818485555765E-5 0.0 4 0.0 0.0 0.0 3.420818485555765E-5 0.0 5 0.0 0.0 0.0 3.420818485555765E-5 0.0 6 0.0 0.0 0.0 3.420818485555765E-5 0.0 7 0.0 0.0 0.0 3.420818485555765E-5 0.0 8 0.0 0.0 0.0 3.420818485555765E-5 0.0 9 0.0 0.0 0.0 3.420818485555765E-5 0.0 10-11 0.0 0.0 0.0 3.420818485555765E-5 0.0 12-13 0.0 0.0 0.0 3.420818485555765E-5 0.0 14-15 0.0 0.0 0.0 1.0262455456667295E-4 0.0 16-17 0.0 0.0 0.0 1.368327394222306E-4 0.0 18-19 0.0 0.0 0.0 1.368327394222306E-4 0.0 20-21 0.0 0.0 0.0 1.368327394222306E-4 0.0 22-23 0.0 0.0 0.0 3.420818485555765E-4 0.0 24-25 0.0 0.0 0.0 6.670596046833741E-4 0.0 26-27 0.0 0.0 0.0 0.0016077846882112095 0.0 28-29 0.0 0.0 0.0 0.0042931271993724845 0.0 30-31 0.0 0.0 0.0 0.014213500807484203 0.0 32-33 0.0 0.0 0.0 0.03234383878092976 0.0 34-35 0.0 0.0 0.0 0.06330224607520943 0.0 36-37 0.0 0.0 0.0 0.1122712626959402 0.0 38-39 0.0 0.0 0.0 0.18619515016880028 0.0 40-41 0.0 0.0 0.0 0.26675542550363857 0.0 42-43 0.0 0.0 0.0 0.35248113675166604 0.0 44-45 0.0 0.0 0.0 0.4574147437960891 0.0 46-47 0.0 0.0 0.0 0.5839337154843691 0.0 48-49 0.0 0.0 0.0 0.7146089816325993 0.0 50-51 0.0 0.0 0.0 0.8545546658766856 0.0 52-53 0.0 0.0 0.0 0.9923794426597274 0.0 54-55 0.0 0.0 0.0 1.1459057762914702 0.0 56-57 0.0 0.0 0.0 1.3295524166885313 0.0 58-59 0.0 0.0 0.0 1.524932464491049 0.0 60-61 0.0 0.0 0.0 1.7362364223438287 3.420818485555765E-5 62-63 0.0 0.0 0.0 1.950037577691064 3.420818485555765E-5 64-65 0.0 0.0 0.0 2.1724420915294718 3.420818485555765E-5 66-67 0.0 0.0 0.0 2.428644292005171 5.131227728333647E-5 68-69 0.0 0.0 0.0 2.7163180225479833 6.84163697111153E-5 70-71 0.0 0.0 0.0 3.022361548358229 6.84163697111153E-5 72-73 0.0 0.0 0.0 3.327926159580498 6.84163697111153E-5 74-75 0.0 0.0 0.0 3.64979097088644 6.84163697111153E-5 76-77 0.0 0.0 0.0 3.998799976875267 6.84163697111153E-5 78-79 0.0 0.0 0.0 4.391800708588342 6.84163697111153E-5 80-81 0.0 0.0 0.0 4.8106286198673605 6.84163697111153E-5 82-83 0.0 0.0 0.0 5.235494275773387 6.84163697111153E-5 84-85 0.0 0.0 0.0 5.660873054452247 6.84163697111153E-5 86-87 0.0 0.0 0.0 6.105938643515479 6.84163697111153E-5 88-89 0.0 0.0 0.0 6.592532968993359 6.84163697111153E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16990 0.0 25.03337 1 TACATGG 11885 0.0 23.577147 2 GTACATG 12200 0.0 23.514332 1 ACCGTGC 430 0.0 23.194366 8 GGTATCA 12675 0.0 22.220247 1 ACATGGG 12455 0.0 21.800884 3 GAGTACT 9315 0.0 21.617958 12-13 ATCAACG 19880 0.0 21.121563 3 TATCAAC 20060 0.0 21.059942 2 TCAACGC 20060 0.0 20.955742 4 CAACGCA 20290 0.0 20.741632 5 GTACTTT 9685 0.0 20.644964 14-15 AGTACTT 9730 0.0 20.6227 12-13 AACGCAG 20635 0.0 20.369036 6 GCACCGT 570 0.0 19.997147 6 AGAGTAC 16005 0.0 19.733206 10-11 TGCACCG 465 0.0 19.430428 5 CATGGGG 6070 0.0 19.037035 4 CCGTGCG 285 4.285903E-8 18.330717 9 TACGGTA 130 0.0075169685 18.289776 4 >>END_MODULE