FastQCFastQC Report
Thu 26 May 2016
SRR938340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922472
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85950.29410033697499927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.22159322655614838No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60130.20575047425604076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.15486889181487454No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32170.110078043519322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161150.026.526521
GTACATG120500.024.864241
TACATGG119150.024.4507662
GGTATCA117200.022.9590591
TATCAAC186150.022.6968062
ATCAACG187000.022.644473
TCAACGC188250.022.418374
ACATGGG127050.022.3693083
CAACGCA189950.022.191575
GAGTACT85300.021.8287512-13
AACGCAG194500.021.6676146
AGTACTT88900.020.8646512-13
GTATAGG7550.020.853051
AGAGTAC151450.020.44891210-11
GTACTTT91450.020.25862314-15
ACGCAGA215500.019.3981677
GTAAGCG4200.019.24406294-95
CGCAGAG220150.018.9237068
CATGGGG63850.018.9059664
GCAGAGT222150.018.3895029
Produced by FastQC (version 0.11.5)