##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2922472 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.030847857567156 31.0 31.0 34.0 28.0 34.0 2 31.20042245058293 31.0 31.0 34.0 28.0 34.0 3 31.25762094555568 31.0 31.0 34.0 27.0 34.0 4 34.91550783035731 37.0 35.0 37.0 32.0 37.0 5 34.910466550235554 37.0 35.0 37.0 32.0 37.0 6 34.8846692115442 37.0 35.0 37.0 32.0 37.0 7 34.79757718807913 37.0 35.0 37.0 32.0 37.0 8 34.751593513984055 37.0 35.0 37.0 32.0 37.0 9 36.21787787872732 39.0 35.0 39.0 32.0 39.0 10-11 36.04253607904541 38.0 35.0 39.0 30.0 39.0 12-13 35.85286206334911 38.0 35.0 39.0 30.0 39.0 14-15 36.9944221877917 39.0 36.0 41.0 31.0 41.0 16-17 36.929786495815875 39.0 36.0 41.0 31.0 41.0 18-19 36.92314212077994 39.0 36.0 41.0 30.5 41.0 20-21 36.85128001226359 39.0 36.0 41.0 30.0 41.0 22-23 36.68770256823676 39.0 36.0 41.0 30.0 41.0 24-25 36.53912954512481 39.0 36.0 41.0 30.0 41.0 26-27 36.313643210268566 39.0 35.5 40.5 29.0 41.0 28-29 36.07233482476479 39.0 35.0 40.0 28.5 41.0 30-31 35.80537760498646 38.0 35.0 40.0 27.0 41.0 32-33 35.50391004601583 38.0 34.0 40.0 27.0 41.0 34-35 35.18727519031833 38.0 34.0 40.0 25.5 41.0 36-37 34.959083440320384 38.0 34.0 40.0 25.0 41.0 38-39 34.70232186997856 38.0 33.5 40.0 24.5 41.0 40-41 34.421355790577294 38.0 33.0 40.0 24.0 41.0 42-43 33.9918507345836 38.0 33.0 40.0 23.0 41.0 44-45 33.501811651232245 37.5 32.5 40.0 20.0 41.0 46-47 32.995198243131156 37.0 31.5 40.0 17.0 41.0 48-49 32.49439464261762 37.0 31.0 40.0 14.5 41.0 50-51 31.761729795871442 36.0 30.0 38.5 11.5 40.5 52-53 31.764564040305604 36.0 30.0 39.0 9.0 40.0 54-55 31.71044735415771 36.0 30.0 39.0 7.5 40.0 56-57 31.253079584680364 36.0 29.5 39.0 4.5 40.0 58-59 30.76894748692203 35.5 28.5 39.0 2.0 40.0 60-61 30.18124724548259 35.0 28.0 38.5 2.0 40.0 62-63 29.42967922361617 34.5 26.5 38.0 2.0 40.0 64-65 28.744878992852627 34.0 26.0 38.0 2.0 40.0 66-67 27.9987086274907 34.0 24.5 37.5 2.0 39.5 68-69 27.11421546553739 33.0 22.5 36.5 2.0 39.0 70-71 26.23838979466698 32.0 20.0 36.0 2.0 39.0 72-73 25.301354811953715 31.0 15.5 35.0 2.0 38.0 74-75 24.321874426855075 31.0 7.5 35.0 2.0 37.5 76-77 23.336915460610058 30.0 2.0 34.0 2.0 36.5 78-79 22.31196073050486 29.0 2.0 34.0 2.0 36.0 80-81 21.28700600039966 28.5 2.0 34.0 2.0 35.5 82-83 20.25245887727923 26.5 2.0 33.5 2.0 35.0 84-85 19.234075467617824 25.5 2.0 33.0 2.0 35.0 86-87 18.210778067334775 24.0 2.0 32.0 2.0 34.5 88-89 17.097444218456154 19.0 2.0 31.5 2.0 34.0 90-91 15.923763341445188 11.0 2.0 31.0 2.0 34.0 92-93 14.75060992885475 2.0 2.0 30.5 2.0 34.0 94-95 13.508378181210976 2.0 2.0 29.5 2.0 34.0 96-97 12.110566670955274 2.0 2.0 29.0 2.0 34.0 98-99 10.482643631829493 2.0 2.0 26.0 2.0 33.5 100-101 7.3863344114160885 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.662926114846556 1101 2 0.5631914139358045 1101 3 0.6024751743517243 1101 4 0.4650056757302323 1101 5 0.5447176999890573 1101 6 0.5731604255738887 1101 7 0.5235392793579123 1101 8 0.5193312374998698 1101 9 0.5883251573367971 1101 10-11 0.639736186056254 1101 12-13 0.538742650363865 1101 14-15 0.6680373732371621 1101 16-17 0.789826992003988 1101 18-19 0.6644647521551903 1101 20-21 0.9121950308982392 1101 22-23 0.9324642652915074 1101 24-25 1.0112718729245245 1101 26-27 1.0351439837683856 1101 28-29 0.9603664284040292 1101 30-31 1.0003187862275453 1101 32-33 1.0191633468709753 1101 34-35 1.1844569094952675 1101 36-37 1.295671195012524 1101 38-39 1.3072418551154357 1101 40-41 1.3910312267340146 1101 42-43 1.5027345935698904 1101 44-45 1.6244701809088653 1101 46-47 1.6575009572701376 1101 48-49 1.6001323337530096 1101 50-51 1.6369426588106428 1101 52-53 1.5946871167968943 1101 54-55 1.7775435773362744 1101 56-57 2.2393760881674467 1101 58-59 2.1753175810585113 1101 60-61 2.2543284655929767 1101 62-63 2.2162070414460437 1101 64-65 2.1649318191270446 1101 66-67 2.5428445296649294 1101 68-69 2.646718919136294 1101 70-71 2.828640572082133 1101 72-73 3.1443633078697673 1101 74-75 3.2019923915992337 1101 76-77 3.6089343602464687 1101 78-79 3.7168811738312932 1101 80-81 3.8296835939782135 1101 82-83 3.7108575334067915 1101 84-85 3.8175744697719516 1101 86-87 3.9886666327558515 1101 88-89 4.205698500089458 1101 90-91 4.389540843855732 1101 92-93 4.2402320764262385 1101 94-95 3.968676182792061 1101 96-97 4.069989603726173 1101 98-99 3.941352624346406 1101 100-101 3.0280534091424167 1102 1 0.675582554250834 1102 2 0.5171626672000649 1102 3 0.5183517455700084 1102 4 0.39224410257588715 1102 5 0.3901660829472604 1102 6 0.5266390697438155 1102 7 0.6142885405706409 1102 8 0.6417434600692005 1102 9 0.4939400922133217 1102 10-11 0.6476136348213686 1102 12-13 0.7726524804048793 1102 14-15 0.8337151568450238 1102 16-17 0.7905640100230187 1102 18-19 0.8655458130162899 1102 20-21 0.8446724527708014 1102 22-23 0.7042387751846348 1102 24-25 0.8381800570597306 1102 26-27 0.8401651844101679 1102 28-29 0.7890696406500552 1102 30-31 0.8110578161338964 1102 32-33 0.8372130344218434 1102 34-35 0.9288763232980273 1102 36-37 1.089033559172215 1102 38-39 1.068086458556273 1102 40-41 1.2556093471055405 1102 42-43 1.3774789372851615 1102 44-45 1.1723624509142567 1102 46-47 1.352135511586546 1102 48-49 1.3722864736408553 1102 50-51 1.423804680747299 1102 52-53 1.4572653554552524 1102 54-55 1.4689214522373248 1102 56-57 1.7791908580489917 1102 58-59 1.8676284813427024 1102 60-61 1.913245322074637 1102 62-63 1.8138477749862254 1102 64-65 2.136055549186363 1102 66-67 2.3752836346884756 1102 68-69 2.3528400610390534 1102 70-71 2.4341449795307675 1102 72-73 2.5003093953555258 1102 74-75 2.651524223397363 1102 76-77 2.520147447421845 1102 78-79 2.9172976294519906 1102 80-81 3.2254894407856334 1102 82-83 3.3459313767485064 1102 84-85 3.548709614006075 1102 86-87 3.844965685146814 1102 88-89 3.935046704201998 1102 90-91 3.879177475774375 1102 92-93 3.754261831127632 1102 94-95 3.6241433854902088 1102 96-97 3.3427686671572427 1102 98-99 3.052987854009915 1102 100-101 2.215385520178377 1103 1 0.5778016068386727 1103 2 0.4981402331059712 1103 3 0.5646628318696365 1103 4 0.4057847904027838 1103 5 0.5038893007951586 1103 6 0.4791617788264233 1103 7 0.5226915465921351 1103 8 0.4647745885743291 1103 9 0.5794912359365867 1103 10-11 0.7601469001504526 1103 12-13 0.6606685831867694 1103 14-15 0.7771929602299466 1103 16-17 0.8067633667106477 1103 18-19 0.828492023898626 1103 20-21 0.8219097851532382 1103 22-23 1.0285896780676538 1103 24-25 1.0264330786452334 1103 26-27 1.1184105725677895 1103 28-29 1.0753268399175937 1103 30-31 1.1057815683704675 1103 32-33 1.2513657117480292 1103 34-35 1.0710916639062518 1103 36-37 1.19599666297173 1103 38-39 1.2042212404384927 1103 40-41 1.3298283577996415 1103 42-43 1.5026046793766312 1103 44-45 1.5181469914447376 1103 46-47 1.682068023705277 1103 48-49 1.6628851545728196 1103 50-51 1.8750083461566653 1103 52-53 2.0654354615486987 1103 54-55 1.9459736727756791 1103 56-57 1.9861390798305152 1103 58-59 2.0941429032603374 1103 60-61 2.224231758734959 1103 62-63 2.4144024772411328 1103 64-65 2.5303654546672796 1103 66-67 2.6558099174039533 1103 68-69 2.74381259482562 1103 70-71 2.7188524760246047 1103 72-73 3.0136269746966526 1103 74-75 2.9782351805084133 1103 76-77 3.060833691127435 1103 78-79 3.095226281501642 1103 80-81 3.2057204701097852 1103 82-83 3.4763418184204937 1103 84-85 3.6897326881974415 1103 86-87 3.726553292006276 1103 88-89 4.0759691847201935 1103 90-91 4.0503998423513625 1103 92-93 3.9400009851567646 1103 94-95 3.736390214597394 1103 96-97 3.7004951137454096 1103 98-99 3.152113906894421 1103 100-101 2.2564926497610527 1104 1 0.546597874554319 1104 2 0.5483208182701915 1104 3 0.5114561476176576 1104 4 0.5388202916976255 1104 5 0.5208833947975222 1104 6 0.6174456044866332 1104 7 0.5711734098194725 1104 8 0.5345052564747661 1104 9 0.6250480571885575 1104 10-11 0.6847743351169981 1104 12-13 0.691665560868735 1104 14-15 0.7791028479923838 1104 16-17 0.9002817560115162 1104 18-19 0.7309536926609113 1104 20-21 0.7642465624487969 1104 22-23 0.7819211827484764 1104 24-25 0.9516954433037483 1104 26-27 1.039491842422585 1104 28-29 1.0557827151970756 1104 30-31 0.9485156765666503 1104 32-33 1.0710750682846282 1104 34-35 1.0047553462945231 1104 36-37 1.1817265279149751 1104 38-39 1.2788218987075695 1104 40-41 1.2883375958175591 1104 42-43 1.2353448235010234 1104 44-45 1.1879311898246598 1104 46-47 1.3134007928603566 1104 48-49 1.4924213986718087 1104 50-51 1.550537045574547 1104 52-53 1.540329758329836 1104 54-55 1.7534185112893823 1104 56-57 1.9250896418203496 1104 58-59 2.0001511381546457 1104 60-61 2.177326478122737 1104 62-63 2.2847833250621825 1104 64-65 2.555996609039692 1104 66-67 2.5771260132812586 1104 68-69 2.8725557468313276 1104 70-71 3.096645095372253 1104 72-73 3.156513081039442 1104 74-75 3.113540513917826 1104 76-77 3.254119798020195 1104 78-79 3.3501093909625332 1104 80-81 3.5803990523495344 1104 82-83 3.756578919946687 1104 84-85 3.879536850513034 1104 86-87 3.8798352191218726 1104 88-89 3.9385748793457687 1104 90-91 3.8658713883578972 1104 92-93 4.013446991441555 1104 94-95 4.057152077522241 1104 96-97 4.000523024757404 1104 98-99 3.6898205268685764 1104 100-101 2.4002212997274013 1105 1 0.6238872975719119 1105 2 0.38575631172094305 1105 3 0.3188894522636332 1105 4 0.3750450438676367 1105 5 0.41644263772935375 1105 6 0.4520465791946222 1105 7 0.5137914035607949 1105 8 0.48706890096827493 1105 9 0.4755639495338002 1105 10-11 0.5693779092376232 1105 12-13 0.4529438383113913 1105 14-15 0.6182933036997795 1105 16-17 0.6203900836355274 1105 18-19 0.6723447194885139 1105 20-21 0.5910738584669346 1105 22-23 0.6807051322640163 1105 24-25 0.7928102921973021 1105 26-27 0.6243707776766811 1105 28-29 0.6937226713039735 1105 30-31 0.7755019727502415 1105 32-33 0.7909073393908912 1105 34-35 0.806325844075964 1105 36-37 0.9800897037194929 1105 38-39 0.9930502004279589 1105 40-41 1.0306407129382578 1105 42-43 1.1811163297221299 1105 44-45 1.1191614934791048 1105 46-47 1.3283915116879044 1105 48-49 1.3037737455771605 1105 50-51 1.2202789663958846 1105 52-53 1.3561678856754504 1105 54-55 1.428105276548301 1105 56-57 1.6395760635594314 1105 58-59 1.7272065754028922 1105 60-61 1.8595895102034632 1105 62-63 1.8611628432800877 1105 64-65 2.0981692367500493 1105 66-67 2.2784751360319255 1105 68-69 2.2487165832527722 1105 70-71 2.44815202068213 1105 72-73 2.5238131213606927 1105 74-75 2.5952275691409383 1105 76-77 2.7745562035281246 1105 78-79 2.8989385570124604 1105 80-81 3.0715275495391374 1105 82-83 3.369857459207033 1105 84-85 3.4259274253408556 1105 86-87 3.5817238717640727 1105 88-89 3.7130819043273924 1105 90-91 3.6299227589326986 1105 92-93 3.5358083972171492 1105 94-95 3.5686566583937225 1105 96-97 3.5030139905567506 1105 98-99 2.998584940501244 1105 100-101 2.245799467045466 1106 1 0.369745842833332 1106 2 0.30074475675230516 1106 3 0.10510600364159473 1106 4 0.25580953346772617 1106 5 0.2480365274497629 1106 6 0.00463517054977558 1106 7 0.17612925231401277 1106 8 -0.12908267464402456 1106 9 0.27075196733797924 1106 10-11 0.35209447736190924 1106 12-13 0.5788919633964795 1106 14-15 0.5401835208409693 1106 16-17 0.06627116032458247 1106 18-19 0.43087311495943226 1106 20-21 0.41237424589439087 1106 22-23 0.39742761641046087 1106 24-25 0.44687746499464964 1106 26-27 0.36136986119563375 1106 28-29 0.5105093787186092 1106 30-31 0.3539085680209908 1106 32-33 0.4493622684886631 1106 34-35 0.409153157646891 1106 36-37 0.5576704003755708 1106 38-39 0.5753156289100332 1106 40-41 0.8188497515205029 1106 42-43 0.40093133930668046 1106 44-45 0.8779652247357248 1106 46-47 1.1927721083879632 1106 48-49 0.9933391717538953 1106 50-51 0.877795665967998 1106 52-53 1.1024467747281754 1106 54-55 1.0345205459837246 1106 56-57 1.5080985499677126 1106 58-59 1.5973339910611877 1106 60-61 1.9289028475472136 1106 62-63 1.9061723604159404 1106 64-65 1.8627657306337255 1106 66-67 2.0284070632823834 1106 68-69 2.2843302667018754 1106 70-71 2.124461746907887 1106 72-73 2.2366941710816377 1106 74-75 2.5964417869067518 1106 76-77 2.720991757772971 1106 78-79 2.8918987907825446 1106 80-81 2.7085825157833163 1106 82-83 3.071514008479742 1106 84-85 3.1143139750354827 1106 86-87 3.0453398027013314 1106 88-89 2.935512332019993 1106 90-91 2.7559104118843383 1106 92-93 2.870413146824699 1106 94-95 2.5959118020185254 1106 96-97 2.3283004941880954 1106 98-99 2.0654804446984816 1106 100-101 1.6871728989255237 1107 1 0.25866115768610953 1107 2 0.2502831203307849 1107 3 0.24861094215089352 1107 4 0.27444518611982716 1107 5 0.2946676574868974 1107 6 0.17587447275332835 1107 7 0.2545501877176335 1107 8 0.33451272874831517 1107 9 0.45106638597306414 1107 10-11 0.43144095069119004 1107 12-13 0.39310815672379107 1107 14-15 0.5457811062629006 1107 16-17 0.5355824367867328 1107 18-19 0.5159535505599919 1107 20-21 0.5977556858074138 1107 22-23 0.5485852309261219 1107 24-25 0.5791735477859348 1107 26-27 0.5759681928364486 1107 28-29 0.7055759343962222 1107 30-31 0.6466190602611945 1107 32-33 0.7987413527157585 1107 34-35 0.6864403248213122 1107 36-37 0.6859349507000445 1107 38-39 0.7902884856699828 1107 40-41 0.7547866085673647 1107 42-43 0.9994015591329486 1107 44-45 0.863558198197758 1107 46-47 0.8769912777821744 1107 48-49 0.7228537016297096 1107 50-51 1.1828804303931157 1107 52-53 1.2177544232829867 1107 54-55 1.412896148275479 1107 56-57 1.5228451816010988 1107 58-59 1.4869357517854134 1107 60-61 1.671421625711318 1107 62-63 1.9131788586862974 1107 64-65 1.8869432854266996 1107 66-67 1.9899343204057516 1107 68-69 2.1103753821141282 1107 70-71 2.369012021045801 1107 72-73 2.2261085276786297 1107 74-75 2.396240572067441 1107 76-77 2.5552163381978765 1107 78-79 2.8809622168710156 1107 80-81 3.015202188277538 1107 82-83 3.020181730926584 1107 84-85 2.8670496914874057 1107 86-87 2.7613187336578413 1107 88-89 3.0708598271532104 1107 90-91 2.9068970441217505 1107 92-93 2.779921837820872 1107 94-95 2.7084038270401383 1107 96-97 2.6394728254488786 1107 98-99 2.392891216173039 1107 100-101 1.8700133565728825 1108 1 0.20360471914745304 1108 2 0.21600707789697893 1108 3 0.11124013125909826 1108 4 0.08565666727906063 1108 5 0.08423322170272485 1108 6 0.2940833608931115 1108 7 0.1934413796005714 1108 8 0.1725652764924206 1108 9 0.2460901979191732 1108 10-11 0.2380124809269759 1108 12-13 0.2233234385824261 1108 14-15 0.3363768427376215 1108 16-17 0.30463328436446346 1108 18-19 0.23225824223970193 1108 20-21 0.3068942192506867 1108 22-23 0.3581504003429714 1108 24-25 0.44676262683633183 1108 26-27 0.3138047652929501 1108 28-29 0.37456189321869005 1108 30-31 0.5085440393444429 1108 32-33 0.610463238093466 1108 34-35 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94-95 -0.37406408136106784 2315 96-97 -0.4603196377160863 2315 98-99 -0.7144242278888111 2315 100-101 -0.3706903786314024 2316 1 -0.9715601491563035 2316 2 -0.8325331134640024 2316 3 -0.6092723161986378 2316 4 -0.6359853231502512 2316 5 -0.6178933347254869 2316 6 -0.5126700892390232 2316 7 -0.645162007970228 2316 8 -0.5719627888147372 2316 9 -0.7475679605818897 2316 10-11 -0.8197050486805395 2316 12-13 -0.693387713207521 2316 14-15 -0.9924558658641018 2316 16-17 -1.0586295870025495 2316 18-19 -0.9728852784954825 2316 20-21 -1.011123975120647 2316 22-23 -1.0944442418670164 2316 24-25 -0.9783306861105601 2316 26-27 -1.2690782939432452 2316 28-29 -1.0839976352830973 2316 30-31 -1.0489343551702675 2316 32-33 -1.2634620427712306 2316 34-35 -0.8534511645302487 2316 36-37 -1.1632827650777031 2316 38-39 -1.2722546810038367 2316 40-41 -1.0383188015498064 2316 42-43 -0.9580137324775038 2316 44-45 -1.0290426935559296 2316 46-47 -0.8843599880959303 2316 48-49 -0.6295752067851623 2316 50-51 -0.6092573681714093 2316 52-53 -0.6687127880558528 2316 54-55 -0.8620227097670146 2316 56-57 -0.8828400262743443 2316 58-59 -0.6826799952900551 2316 60-61 -0.6867592899969281 2316 62-63 -0.40677504388826335 2316 64-65 -0.38303156811517525 2316 66-67 -0.30460258905375426 2316 68-69 -0.21845505595974046 2316 70-71 -0.31184949544401164 2316 72-73 -0.22676281431161271 2316 74-75 -0.2812626977137427 2316 76-77 -0.13455148482445267 2316 78-79 -0.07902932857934175 2316 80-81 -0.2897927766037718 2316 82-83 -0.22543623255463174 2316 84-85 0.0738904604276378 2316 86-87 0.14186595915116484 2316 88-89 -0.3426428726345243 2316 90-91 -0.034563304517179816 2316 92-93 0.1695312174974255 2316 94-95 -0.12116596034065275 2316 96-97 0.05232819067112082 2316 98-99 4.735063283103358E-4 2316 100-101 0.23777789630582102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10102.0 3 3995.0 4 4182.0 5 4872.0 6 5769.0 7 7589.0 8 9500.0 9 12375.0 10 15863.0 11 19874.0 12 24604.0 13 29242.0 14 33705.0 15 38759.0 16 44630.0 17 50843.0 18 51474.0 19 51570.0 20 56201.0 21 63473.0 22 69008.0 23 76820.0 24 85193.0 25 94308.0 26 103161.0 27 114147.0 28 123945.0 29 136738.0 30 149936.0 31 165439.0 32 182393.0 33 202790.0 34 224446.0 35 237968.0 36 227618.0 37 154090.0 38 35421.0 39 429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.297240963797485 18.70258453975187 14.52013132575396 23.48004317069669 2 15.278527462891015 20.117832815408836 40.15659928456937 24.447040437130788 3 19.71838844389676 23.87776408571233 29.199767234887382 27.204080235503525 4 12.839821288398227 16.34014359506643 36.896770463221266 33.92326465331408 5 13.935236767371581 37.23790003650003 33.85719656529977 14.969666630828618 6 32.374189881781795 35.65071365723227 17.441310294535533 14.533786166450408 7 29.173039295558077 32.0880634556376 20.29987526306349 18.43902198574083 8 26.41531974965948 34.683485200600686 19.667025068625247 19.23416998111458 9 26.47968349074817 16.928302083133655 20.085350424869443 36.50666400124873 10-11 25.233873758660835 26.425963281931143 27.093858608084435 21.246304351323587 12-13 26.296426299541054 23.66786527630003 27.375226311657784 22.660482112501132 14-15 22.821291797001077 25.284060279615872 26.088554040164276 25.806093883218768 16-17 22.411573185644716 28.4007621269324 26.603356876853933 22.584307810568948 18-19 22.38826710714587 27.158643876705295 28.67948823043287 21.77360078571596 20-21 23.207059915251033 26.150921896187562 28.650672448267585 21.991345740293823 22-23 22.664846590214786 26.191712513721598 28.538512381871023 22.60492851419259 24-25 22.64057418387392 26.269548951133338 28.718966994833846 22.3709098701589 26-27 22.312565240630637 26.49409280801151 28.7775212984612 22.415820652896652 28-29 22.104913486566417 27.051807086021913 28.666486054189562 22.176793373222107 30-31 22.39282425191724 26.437645807229725 28.564585104356517 22.604944836496514 32-33 22.18221011933122 26.5790405036117 28.491304127578708 22.74744524947837 34-35 22.8437694672061 26.52949773752559 28.308689133961764 22.318043661306554 36-37 22.690173256473457 26.19646298981998 28.538182520185646 22.57518123352092 38-39 22.298915125664625 26.636153773891458 28.473977201229207 22.59095389921471 40-41 22.69231118030777 26.51303092477978 28.34436390323214 22.45029399168032 42-43 22.4051528968447 26.52051985491864 28.128224902462605 22.94610234577405 44-45 22.489481973056602 26.558711020674675 28.149265295199456 22.802541711069267 46-47 22.783053827342012 26.888019463001456 27.71781865026337 22.61110805939316 48-49 22.624152080277064 26.7167374446449 27.795134073175255 22.863976401902782 50-51 22.567717079477546 27.03236792726927 27.764969349318523 22.63494564393466 52-53 22.85300700162208 27.06051646373598 27.41367745997851 22.672799074663434 54-55 22.43622757574959 26.85018994075409 27.696890077328014 23.01669240616831 56-57 22.60690680141872 26.91242046036103 27.5823252052858 22.898347532934444 58-59 22.898673912872383 26.570436123991698 27.51489636618661 23.01599359694931 60-61 22.52567616261781 26.791342873656475 27.972259854776933 22.710721108948782 62-63 22.453706040608605 26.8721845088548 27.829676078743663 22.844433371792928 64-65 22.20516528593315 26.982495271605096 27.704373256260013 23.107966186201743 66-67 22.328745443548765 26.788691018497847 27.548657039198527 23.33390649875486 68-69 22.514574156758933 26.60440626137804 27.672299452211547 23.208720129651482 70-71 22.54310523313255 26.6720292612179 27.468478929315392 23.316386576334157 72-73 22.611450309710385 26.527296280789024 27.575115098717028 23.28613831078356 74-75 22.370547739173258 27.028197495054133 27.33035629037496 23.270898475397644 76-77 22.553093054085075 26.59080689944503 27.36294973654512 23.49315030992478 78-79 22.70688015517145 26.716514035549405 27.26586286230142 23.310742946977726 80-81 22.732620653922943 26.856547203090358 27.139697986186732 23.271134156799967 82-83 22.674573004376388 26.792307028915374 27.159207732669323 23.37391223403891 84-85 22.778543652491777 26.577947633333064 27.17935652721739 23.464152186957765 86-87 22.745025845142667 26.71712965275872 27.015719239558088 23.522125262540523 88-89 22.714609391750617 26.669827743013734 26.83628904238723 23.77927382284842 90-91 22.77441112935192 26.569544502194002 26.83329365915333 23.822750709300752 92-93 22.9969389134426 26.323753107712943 26.982340148485797 23.696967830358656 94-95 22.794834985568222 26.186144705213355 26.946861244269684 24.072159064948735 96-97 22.751686994102833 26.176412507833053 26.899431981718823 24.172468516345287 98-99 22.903630779002317 26.21681735809166 26.372097312878246 24.50745455002778 100-101 23.74998008588167 25.25934177714791 26.635719984027595 24.354958152942828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 515.0 1 391.0 2 347.0 3 430.0 4 362.5 5 333.5 6 586.0 7 1258.5 8 2027.5 9 2327.0 10 2046.5 11 1765.0 12 1847.5 13 1994.5 14 2196.0 15 2436.0 16 2652.5 17 2907.5 18 3267.5 19 3552.0 20 3893.5 21 4589.5 22 5547.5 23 6688.0 24 8046.5 25 9905.5 26 12565.0 27 16333.5 28 20522.5 29 25233.0 30 30552.5 31 35863.0 32 41994.0 33 47026.5 34 51301.0 35 56843.0 36 63233.0 37 69076.0 38 73840.5 39 78016.5 40 82555.0 41 88163.5 42 93182.0 43 98277.5 44 103052.5 45 107672.0 46 111844.5 47 117608.5 48 125269.0 49 125611.0 50 118756.0 51 111766.0 52 106067.0 53 101294.0 54 97530.5 55 92214.0 56 84058.0 57 76581.5 58 70235.0 59 62487.5 60 53228.0 61 44107.5 62 35906.5 63 27950.5 64 21404.5 65 16853.5 66 12906.5 67 9748.0 68 7459.5 69 5615.5 70 4321.5 71 3437.5 72 2726.5 73 2243.0 74 1850.5 75 1441.0 76 1020.0 77 690.0 78 468.5 79 318.5 80 181.0 81 91.5 82 57.5 83 41.0 84 28.0 85 23.0 86 20.5 87 16.0 88 10.0 89 9.0 90 7.0 91 3.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.384537473755095 2 0.029940406614674155 3 0.03668127530392079 4 0.04588581173746062 5 0.06590311215984276 6 0.05481660731052342 7 0.038460590897021425 8 0.04006881845232393 9 0.037776238745828875 10-11 0.03870011414993882 12-13 0.030539214746967636 14-15 0.028092655806454262 16-17 0.022207227306198316 18-19 0.027562282889280032 20-21 0.03491906851459997 22-23 0.03363590823111393 24-25 0.04268646543063543 26-27 0.036801036930379484 28-29 0.03187370144179311 30-31 0.03491906851459997 32-33 0.02929027207104123 34-35 0.03052210594318782 36-37 0.033019991295040636 38-39 0.014662244839300426 40-41 0.012506535563043888 42-43 0.013259322929355695 44-45 0.01183929221563115 46-47 0.021232025490748926 48-49 0.018528834493538347 50-51 0.012010380253429289 52-53 0.00999154140741126 54-55 0.008383313852108762 56-57 0.00908477480708113 58-59 0.003935024869357174 60-61 0.007151479979962169 62-63 0.012232794702566867 64-65 0.00797270256139323 66-67 0.0035757399899810846 68-69 0.006022298930494458 70-71 0.012968473265098862 72-73 0.01048769671702586 74-75 0.011394463317355993 76-77 0.005132641133944141 78-79 0.009752018154493867 80-81 0.012113033076108172 82-83 0.014645136035520616 84-85 0.008571510693686715 86-87 0.002258362098935422 88-89 0.016133601964364416 90-91 0.02588562011885828 92-93 0.010504805520805674 94-95 0.021266243098308556 96-97 0.019127642625831828 98-99 0.011308919298456923 100-101 0.1262116454836864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2922472.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.219355693762715 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6858716764121 41.0363919741587 2 13.19130588861938 14.30448212080562 3 4.6565079658746935 7.574185850777986 4 2.171799388058561 4.710142540665733 5 1.1798752138270485 3.198603694637154 6 0.706157555701419 2.297244461304798 7 0.46867251411301813 1.7787785222608634 8 0.31979518243504856 1.3871270996478111 9 0.24873649260435418 1.2137699129881012 >10 1.240822731419864 12.030415035328513 >50 0.08136633918028312 3.0471776506559367 >100 0.044264002796497726 4.1709229366456295 >500 0.002983911855603563 1.106442610737139 >1k 0.0016506746434144807 1.41855147267154 >5k 1.90462458855517E-4 0.7257641167145534 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8595 0.29410033697499927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6476 0.22159322655614838 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6013 0.20575047425604076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4526 0.15486889181487454 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3217 0.110078043519322 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.71088037798138E-5 0.0 16-17 0.0 0.0 0.0 3.42176075596276E-5 0.0 18-19 0.0 0.0 0.0 5.132641133944141E-5 0.0 20-21 0.0 0.0 0.0 1.1976162645869661E-4 0.0 22-23 0.0 0.0 0.0 3.0795846803664844E-4 0.0 24-25 0.0 0.0 0.0 6.330257398531107E-4 0.0 26-27 0.0 0.0 0.0 0.0012489426759264076 0.0 28-29 0.0 0.0 0.0 0.004123221710935127 0.0 30-31 0.0 0.0 0.0 0.013225105321796068 0.0 32-33 0.0 0.0 0.0 0.03276335923834343 0.0 34-35 0.0 0.0 0.0 0.06150614958843062 0.0 36-37 0.0 0.0 0.0 0.10997539069664312 0.0 38-39 0.0 0.0 0.0 0.18479218962576888 0.0 40-41 0.0 0.0 0.0 0.2642112567716645 0.0 42-43 0.0 0.0 0.0 0.34831813615322915 0.0 44-45 0.0 0.0 0.0 0.4549402013090288 0.0 46-47 0.0 0.0 0.0 0.5784315469917248 0.0 48-49 0.0 1.71088037798138E-5 0.0 0.7113669523608781 0.0 50-51 0.0 3.42176075596276E-5 0.0 0.8509747912041586 0.0 52-53 0.0 3.42176075596276E-5 0.0 0.9920710959762831 0.0 54-55 0.0 3.42176075596276E-5 0.0 1.1478809719990473 0.0 56-57 0.0 3.42176075596276E-5 0.0 1.3340589747309812 0.0 58-59 0.0 3.42176075596276E-5 0.0 1.52986923399095 0.0 60-61 0.0 3.42176075596276E-5 0.0 1.7413511575132286 0.0 62-63 3.42176075596276E-5 3.42176075596276E-5 0.0 1.9564430386330476 0.0 64-65 3.42176075596276E-5 3.42176075596276E-5 0.0 2.1822279221152505 0.0 66-67 3.42176075596276E-5 3.42176075596276E-5 0.0 2.4367384871437605 0.0 68-69 3.42176075596276E-5 3.42176075596276E-5 0.0 2.7213947644323024 6.84352151192552E-5 70-71 3.42176075596276E-5 3.42176075596276E-5 0.0 3.021722705983154 1.0265282267888281E-4 72-73 3.42176075596276E-5 3.42176075596276E-5 0.0 3.3246340769047573 1.0265282267888281E-4 74-75 3.42176075596276E-5 3.42176075596276E-5 0.0 3.636442025791864 1.0265282267888281E-4 76-77 3.42176075596276E-5 3.42176075596276E-5 0.0 3.966607721134711 1.368704302385104E-4 78-79 3.42176075596276E-5 3.42176075596276E-5 0.0 4.333403365370138 1.368704302385104E-4 80-81 3.42176075596276E-5 3.42176075596276E-5 0.0 4.72926686722747 1.368704302385104E-4 82-83 3.42176075596276E-5 3.42176075596276E-5 0.0 5.126362202956949 1.368704302385104E-4 84-85 3.42176075596276E-5 3.42176075596276E-5 0.0 5.525236854279528 1.368704302385104E-4 86-87 3.42176075596276E-5 3.42176075596276E-5 0.0 5.934034611794399 1.368704302385104E-4 88-89 3.42176075596276E-5 3.42176075596276E-5 0.0 6.362815452124092 1.368704302385104E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16115 0.0 26.52652 1 GTACATG 12050 0.0 24.86424 1 TACATGG 11915 0.0 24.450766 2 GGTATCA 11720 0.0 22.959059 1 TATCAAC 18615 0.0 22.696806 2 ATCAACG 18700 0.0 22.64447 3 TCAACGC 18825 0.0 22.41837 4 ACATGGG 12705 0.0 22.369308 3 CAACGCA 18995 0.0 22.19157 5 GAGTACT 8530 0.0 21.82875 12-13 AACGCAG 19450 0.0 21.667614 6 AGTACTT 8890 0.0 20.86465 12-13 GTATAGG 755 0.0 20.85305 1 AGAGTAC 15145 0.0 20.448912 10-11 GTACTTT 9145 0.0 20.258623 14-15 ACGCAGA 21550 0.0 19.398167 7 GTAAGCG 420 0.0 19.244062 94-95 CGCAGAG 22015 0.0 18.923706 8 CATGGGG 6385 0.0 18.905966 4 GCAGAGT 22215 0.0 18.389502 9 >>END_MODULE