FastQCFastQC Report
Thu 26 May 2016
SRR938340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922472
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87670.2999857654752552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71000.242945013673356No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62860.2150918811198191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.1761522437169629No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34150.11685312981612825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124750.023.996371
GTATCAA165400.023.413571
TACATGG113200.023.332162
GTACATG115600.023.2239531
GAGTACT89500.022.4479712-13
ACATGGG119450.021.3531883
GTACTTT94900.021.1956614-15
AGTACTT92800.020.06309112-13
TATCAAC194700.019.8846652
ATCAACG193050.019.8307823
GGACCGA8400.019.7904626
TCAACGC195100.019.5980664
CAACGCA196350.019.47335
AGAGTAC155450.019.44766210-11
AACGCAG200450.019.1220576
AGGACCG12350.018.4607325
ACTTTTT113250.017.88712516-17
TAAGGTG13050.017.47055
ACGCAGA221650.017.2502487
CGCAGAG224500.016.9889438
Produced by FastQC (version 0.11.5)