##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2922472 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811369963510344 31.0 31.0 34.0 30.0 34.0 2 32.06071059021267 33.0 31.0 34.0 30.0 34.0 3 32.12287440221839 33.0 31.0 34.0 30.0 34.0 4 35.767546789156576 37.0 35.0 37.0 35.0 37.0 5 35.6646400718296 37.0 35.0 37.0 33.0 37.0 6 35.69661266215724 37.0 35.0 37.0 33.0 37.0 7 35.621181999348494 37.0 35.0 37.0 33.0 37.0 8 35.59522246919731 37.0 35.0 37.0 33.0 37.0 9 37.13805846557298 39.0 37.0 39.0 33.0 39.0 10-11 37.063062195292204 39.0 37.0 39.0 33.0 39.0 12-13 36.945670822509165 39.0 37.0 39.0 33.0 39.0 14-15 37.94530400975613 40.0 37.0 41.0 33.0 41.0 16-17 38.04912365285279 40.0 37.0 41.0 33.0 41.0 18-19 38.19138027669726 40.0 37.5 41.0 33.0 41.0 20-21 38.14495913048953 40.0 37.0 41.0 33.0 41.0 22-23 38.01734285221552 40.0 37.0 41.0 33.0 41.0 24-25 37.8979748993318 40.0 37.0 41.0 32.5 41.0 26-27 37.675184056511064 40.0 37.0 41.0 32.0 41.0 28-29 37.5400347719328 39.5 36.5 41.0 32.0 41.0 30-31 37.29321392985116 39.0 36.0 41.0 31.0 41.0 32-33 37.19031662236627 39.0 36.0 41.0 31.0 41.0 34-35 37.04698744761284 39.0 36.0 41.0 31.0 41.0 36-37 36.92511168627107 39.0 36.0 41.0 30.5 41.0 38-39 36.70887881902718 39.0 35.0 40.5 30.0 41.0 40-41 36.49505333156314 39.0 35.0 40.0 30.0 41.0 42-43 36.23608883164663 38.5 35.0 40.0 30.0 41.0 44-45 35.933923746745904 38.0 35.0 40.0 29.0 41.0 46-47 35.51378490538147 38.0 34.5 40.0 27.5 41.0 48-49 35.10479330511978 38.0 34.0 40.0 26.5 41.0 50-51 34.70872466870512 38.0 33.5 40.0 25.5 41.0 52-53 34.11759342775568 37.5 33.0 40.0 24.0 41.0 54-55 33.685495703637194 37.0 32.5 40.0 23.0 41.0 56-57 33.284275948580515 37.0 32.0 39.0 22.0 40.5 58-59 32.74211900062687 36.0 31.0 39.0 20.0 40.0 60-61 32.222728224598896 36.0 31.0 39.0 18.0 40.0 62-63 31.705021468126983 35.0 30.0 38.5 14.5 40.0 64-65 31.03829292461998 35.0 29.0 38.0 10.0 40.0 66-67 30.365006234448096 34.0 28.0 38.0 7.5 40.0 68-69 29.62457809689879 34.0 27.5 37.0 4.5 39.0 70-71 28.823785822413356 33.5 26.0 36.0 2.0 39.0 72-73 28.221489376117205 33.0 26.0 36.0 2.0 38.5 74-75 27.49547780098492 32.0 25.0 35.0 2.0 38.0 76-77 26.322190084284813 30.5 24.0 34.0 2.0 36.0 78-79 26.799354792791856 32.0 25.0 35.0 2.0 36.0 80-81 26.39525357300258 32.0 24.0 35.0 2.0 36.0 82-83 25.69360801403743 31.0 21.0 34.5 2.0 36.0 84-85 24.886068882781426 31.0 19.0 34.0 2.0 35.0 86-87 23.948100101557863 30.5 13.5 34.0 2.0 35.0 88-89 22.944807341182397 29.5 4.5 34.0 2.0 35.0 90-91 22.048889946593157 29.0 2.0 34.0 2.0 35.0 92-93 20.932218683361207 28.5 2.0 33.5 2.0 35.0 94-95 19.721646606023942 26.5 2.0 33.0 2.0 34.0 96-97 18.308209625276135 24.5 2.0 32.0 2.0 34.0 98-99 16.508226426121446 11.0 2.0 32.0 2.0 34.0 100-101 12.314564861528186 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2717511806783044 1101 2 0.29753876669251866 1101 3 0.27370727883572243 1101 4 0.14507216709871074 1101 5 0.15754114320363755 1101 6 0.18534502280023446 1101 7 0.2761226381682391 1101 8 0.2552388064933595 1101 9 0.2731394911889993 1101 10-11 0.3280437545925565 1101 12-13 0.35291671387087575 1101 14-15 0.5084558434824729 1101 16-17 0.44071500169545175 1101 18-19 0.5151720145567538 1101 20-21 0.4475921657711055 1101 22-23 0.4693622834850757 1101 24-25 0.4011212715071437 1101 26-27 0.5378446573579652 1101 28-29 0.4915608314503004 1101 30-31 0.6668729506264057 1101 32-33 0.5736805824570226 1101 34-35 0.3933373340124078 1101 36-37 0.4240692942124298 1101 38-39 0.5331707421042466 1101 40-41 0.6238346586526546 1101 42-43 0.743622922054648 1101 44-45 0.7022099327359754 1101 46-47 0.7115683557558796 1101 48-49 0.737958682842013 1101 50-51 0.9273807350934433 1101 52-53 0.9282948864456984 1101 54-55 0.9619231387726188 1101 56-57 1.1490616778512788 1101 58-59 1.2213624985937273 1101 60-61 1.3603309538061907 1101 62-63 1.2606158364998343 1101 64-65 1.2996377785630706 1101 66-67 1.359372385270433 1101 68-69 1.4804220720741519 1101 70-71 1.7020901814140323 1101 72-73 1.6749916260793896 1101 74-75 1.7114937606020568 1101 76-77 1.7398687269239197 1101 78-79 1.736573529557944 1101 80-81 1.9231331970096903 1101 82-83 2.12209094092006 1101 84-85 2.3359908598040953 1101 86-87 2.363758196138999 1101 88-89 2.3572382943381385 1101 90-91 2.732414543583211 1101 92-93 2.866176563897369 1101 94-95 2.7687594590495017 1101 96-97 2.921624890662109 1101 98-99 3.044441361429289 1101 100-101 2.615885466946523 1102 1 0.18072010359066937 1102 2 0.21377348418410946 1102 3 0.12310032579357966 1102 4 0.16147991700999853 1102 5 0.17330286497478653 1102 6 0.21812817066586376 1102 7 0.1894876359794253 1102 8 0.33245964196242994 1102 9 0.26431827518003814 1102 10-11 0.270730367251673 1102 12-13 0.33262660275201483 1102 14-15 0.4162678048097561 1102 16-17 0.33641418814483615 1102 18-19 0.32382788173579513 1102 20-21 0.22914846676966505 1102 22-23 0.3974053388847736 1102 24-25 0.28079347541343935 1102 26-27 0.3804400021135663 1102 28-29 0.3563209315796101 1102 30-31 0.3141987075358159 1102 32-33 0.2962993267764702 1102 34-35 0.4344142093390886 1102 36-37 0.492577090528556 1102 38-39 0.5551945895405055 1102 40-41 0.5887384722406779 1102 42-43 0.5144235874939653 1102 44-45 0.42207964533938735 1102 46-47 0.5038694240781538 1102 48-49 0.5708689132384919 1102 50-51 0.7424900594329316 1102 52-53 0.7431162025435256 1102 54-55 0.5339509796535609 1102 56-57 0.6725605723242509 1102 58-59 0.7876599991017486 1102 60-61 0.7570458147787136 1102 62-63 0.9863072584795525 1102 64-65 1.0113785564860898 1102 66-67 0.9901732427994929 1102 68-69 1.1829204266738422 1102 70-71 1.427220372765749 1102 72-73 1.5091418463254485 1102 74-75 1.4047063453111086 1102 76-77 1.3439846540956246 1102 78-79 1.5329577686955247 1102 80-81 1.817928424287242 1102 82-83 1.8841537977393976 1102 84-85 1.880111725704225 1102 86-87 2.0032278616298775 1102 88-89 1.97130851426067 1102 90-91 2.5545590284339035 1102 92-93 2.7110532271165297 1102 94-95 2.3565713900468204 1102 96-97 2.499481012410609 1102 98-99 2.3825496414071665 1102 100-101 1.8670237250565016 1103 1 0.2331778773869182 1103 2 0.2550707187592991 1103 3 0.1901492971319314 1103 4 0.1536686843258721 1103 5 0.17287174923263393 1103 6 0.22178924451915805 1103 7 0.2836903227317009 1103 8 0.21509824567353064 1103 9 0.22460837466790906 1103 10-11 0.3169631463698934 1103 12-13 0.33300315921380275 1103 14-15 0.488199344604169 1103 16-17 0.38326745729307277 1103 18-19 0.3621745309434914 1103 20-21 0.35193412722680506 1103 22-23 0.3590803672482892 1103 24-25 0.38966542332101994 1103 26-27 0.4731958936633518 1103 28-29 0.3397619464211985 1103 30-31 0.3615124423962257 1103 32-33 0.34674595781398665 1103 34-35 0.4540937904796962 1103 36-37 0.46035776514430893 1103 38-39 0.471203865592436 1103 40-41 0.5815744003062164 1103 42-43 0.5123867482884421 1103 44-45 0.6594781565530212 1103 46-47 0.66662864764114 1103 48-49 0.6788077513109343 1103 50-51 0.7407922671839557 1103 52-53 0.7156769033964494 1103 54-55 0.8624975886237891 1103 56-57 1.0404846111918928 1103 58-59 1.271308290495071 1103 60-61 1.2273110069808268 1103 62-63 1.1994678093918871 1103 64-65 1.445780033873465 1103 66-67 1.4604352686147983 1103 68-69 1.4000621000751678 1103 70-71 1.6187683851760362 1103 72-73 1.6906983251669203 1103 74-75 1.4892906450013363 1103 76-77 1.531183877795005 1103 78-79 1.5811034107973612 1103 80-81 1.7713339463684044 1103 82-83 1.9053598208890925 1103 84-85 1.9904713882745604 1103 86-87 1.9738626685996294 1103 88-89 1.891906128363857 1103 90-91 2.1005683337140226 1103 92-93 2.425496408253828 1103 94-95 2.4531141584334186 1103 96-97 2.3860243799750194 1103 98-99 2.3345870408195815 1103 100-101 1.937942386425071 1104 1 0.21409492599676483 1104 2 0.24837872135974237 1104 3 0.28965079454168574 1104 4 0.1508699064523853 1104 5 0.1721305491645282 1104 6 0.10361120113609701 1104 7 0.14195841006429788 1104 8 0.16757550839613344 1104 9 0.36118706206663376 1104 10-11 0.3205885416326737 1104 12-13 0.2285852676593123 1104 14-15 0.4559369187614166 1104 16-17 0.40269333831746223 1104 18-19 0.4006304286801097 1104 20-21 0.4078225487170002 1104 22-23 0.4095754668949567 1104 24-25 0.3819248724474704 1104 26-27 0.501540866896967 1104 28-29 0.3996770126158964 1104 30-31 0.42437340362123166 1104 32-33 0.5042050268190295 1104 34-35 0.4527102131999925 1104 36-37 0.5613625157452162 1104 38-39 0.5726298020721998 1104 40-41 0.7103174950233111 1104 42-43 0.6309381988475664 1104 44-45 0.5968427405952994 1104 46-47 0.7195593153582536 1104 48-49 0.6268554585175394 1104 50-51 0.6950934274616856 1104 52-53 0.8170253856721601 1104 54-55 0.9277966959067996 1104 56-57 0.9151155293060711 1104 58-59 0.9485354826953838 1104 60-61 0.911343284301708 1104 62-63 0.9690951744663145 1104 64-65 1.202446251397408 1104 66-67 1.0798131646973808 1104 68-69 1.1734404943395234 1104 70-71 1.3314663373831266 1104 72-73 1.6633685634348438 1104 74-75 1.7229177314239585 1104 76-77 1.6519077125388968 1104 78-79 1.7812942849963775 1104 80-81 1.9470758167132765 1104 82-83 1.9493449290580145 1104 84-85 2.161207322373407 1104 86-87 2.1938664084002504 1104 88-89 2.15853454915284 1104 90-91 2.4256793189429544 1104 92-93 2.6464247289710627 1104 94-95 2.477785957701453 1104 96-97 2.5436736471734633 1104 98-99 2.769895937580692 1104 100-101 2.850981996459197 1105 1 0.17682961404903708 1105 2 0.18376118657204188 1105 3 0.109077270293362 1105 4 0.11291561218408219 1105 5 0.10681586405053167 1105 6 0.08244569073894326 1105 7 0.19447449248124116 1105 8 0.27917022431454797 1105 9 0.2786578633778021 1105 10-11 0.21559208422921472 1105 12-13 0.23762901604985842 1105 14-15 0.3966995610406201 1105 16-17 0.2777007750659095 1105 18-19 0.26310886210959694 1105 20-21 0.19626772357248257 1105 22-23 0.2988200687537983 1105 24-25 0.2923849309546753 1105 26-27 0.30249241748798994 1105 28-29 0.28998902933072657 1105 30-31 0.4188051968550113 1105 32-33 0.33044268112673336 1105 34-35 0.3582764221161696 1105 36-37 0.4379292689375518 1105 38-39 0.3803312159870984 1105 40-41 0.41608208713794426 1105 42-43 0.4757418957311117 1105 44-45 0.46893188135310737 1105 46-47 0.5808552092024328 1105 48-49 0.6539602128598005 1105 50-51 0.7355232897347577 1105 52-53 0.7607245363872082 1105 54-55 0.8009684837474893 1105 56-57 0.9000220481362433 1105 58-59 0.9950952676958167 1105 60-61 0.9751657630728943 1105 62-63 1.136277529278086 1105 64-65 1.268681216885927 1105 66-67 1.1021049768566513 1105 68-69 1.3021768188627796 1105 70-71 1.4053833122540347 1105 72-73 1.4020999441070643 1105 74-75 1.441593843395836 1105 76-77 1.52513474479667 1105 78-79 1.602614965426575 1105 80-81 1.6500179765260476 1105 82-83 1.870941842611483 1105 84-85 2.0722697161134462 1105 86-87 2.034190285280147 1105 88-89 2.0569924958994292 1105 90-91 2.1707875941371952 1105 92-93 2.1498998842707913 1105 94-95 2.1676207862765366 1105 96-97 2.195709815102802 1105 98-99 2.504702812259975 1105 100-101 2.301600827752239 1106 1 0.005913728914080707 1106 2 0.03234227209098606 1106 3 0.02486268845795081 1106 4 -0.005719096392780898 1106 5 0.12512688996558552 1106 6 0.05892840439277336 1106 7 0.028895902383148098 1106 8 -0.03345148562942768 1106 9 -0.07408218440796333 1106 10-11 0.11827904396972855 1106 12-13 0.08108083076496797 1106 14-15 0.06767199222951348 1106 16-17 0.04898944006142614 1106 18-19 0.13785065325370027 1106 20-21 0.03703099090786566 1106 22-23 0.18839182373887553 1106 24-25 0.09760650231731915 1106 26-27 -0.13406258377124658 1106 28-29 0.21313897293691753 1106 30-31 0.2521378233626663 1106 32-33 0.06192314163779855 1106 34-35 -0.11699974011185788 1106 36-37 0.01134371758525532 1106 38-39 0.10735122589571233 1106 40-41 0.15884952061259838 1106 42-43 0.29112098700122857 1106 44-45 0.18537777917612885 1106 46-47 -0.11552548449585487 1106 48-49 0.13178328547667917 1106 50-51 0.5212132559854581 1106 52-53 0.46736094439047093 1106 54-55 0.21698590384682603 1106 56-57 0.41556351357508703 1106 58-59 0.6519786514638852 1106 60-61 0.7374157044607372 1106 62-63 0.7145730441755767 1106 64-65 0.7923642266670861 1106 66-67 0.6628126572461746 1106 68-69 0.5784133179486908 1106 70-71 0.46998614582029674 1106 72-73 0.7915781262471278 1106 74-75 0.8354805516369019 1106 76-77 0.7960309584294016 1106 78-79 1.127491803771413 1106 80-81 1.0017966326525283 1106 82-83 1.1869217900758535 1106 84-85 1.2938252023638377 1106 86-87 1.3852916392971544 1106 88-89 1.2619758017644287 1106 90-91 1.3508707364101156 1106 92-93 1.2751318318343294 1106 94-95 1.085462577812116 1106 96-97 1.1675926703827457 1106 98-99 1.2145370083555989 1106 100-101 1.4125727304530074 1107 1 0.056673601213756086 1107 2 0.07859557376350068 1107 3 0.09130983086747335 1107 4 0.09801602484576932 1107 5 0.013366769765198683 1107 6 -0.058711807367288316 1107 7 0.03078598799068999 1107 8 0.1980138466519037 1107 9 0.19406679007587968 1107 10-11 0.15444014419585983 1107 12-13 0.15438016269143162 1107 14-15 0.3020344280993612 1107 16-17 0.18811726370712734 1107 18-19 0.13431460760976677 1107 20-21 0.20993103910378608 1107 22-23 0.26438440181815537 1107 24-25 0.28488510622106133 1107 26-27 0.2874985489210715 1107 28-29 0.30079185028394306 1107 30-31 0.22618044844242036 1107 32-33 0.2565473770151172 1107 34-35 0.29316503835677565 1107 36-37 0.32783065623800667 1107 38-39 0.36464321895449103 1107 40-41 0.47885535949223623 1107 42-43 0.5041897586846744 1107 44-45 0.42311078382081035 1107 46-47 0.5457108038894276 1107 48-49 0.5413316154186774 1107 50-51 0.5265693898921171 1107 52-53 0.5039815103339507 1107 54-55 0.542440052911914 1107 56-57 0.5997807954004415 1107 58-59 0.7065779960999734 1107 60-61 0.6935844682163932 1107 62-63 0.8991819965371128 1107 64-65 1.03799859451399 1107 66-67 0.9904960251220167 1107 68-69 1.3160145073687417 1107 70-71 1.3530583340033608 1107 72-73 1.5217408787251898 1107 74-75 1.3917500801096203 1107 76-77 1.3272851661349598 1107 78-79 1.4742855478648949 1107 80-81 1.5623070564369037 1107 82-83 1.6579656974440375 1107 84-85 1.9233615022003754 1107 86-87 2.1631617506511347 1107 88-89 2.2452347963445902 1107 90-91 2.411704091765081 1107 92-93 2.6725891822161856 1107 94-95 2.7017127524122344 1107 96-97 2.5205049689005037 1107 98-99 2.471880752260322 1107 100-101 2.2729701832417675 1108 1 0.02155036066324456 1108 2 0.04376084015917314 1108 3 0.09039207508708813 1108 4 0.012016355869526762 1108 5 -0.01198023576046836 1108 6 -0.021993275250139277 1108 7 0.01914832639516817 1108 8 0.026575933093646142 1108 9 -0.06613337300646549 1108 10-11 0.04701078664008662 1108 12-13 0.053458509536511656 1108 14-15 0.1492495009169943 1108 16-17 0.13390594805977685 1108 18-19 -0.06484639394462732 1108 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-0.2814272043018704 2316 56-57 -0.30662940599901134 2316 58-59 -0.2282539538338355 2316 60-61 -0.18606930844046587 2316 62-63 -0.4725850520145656 2316 64-65 -0.33458186011405644 2316 66-67 -0.20490260212610778 2316 68-69 -0.07128152529675802 2316 70-71 -0.23066236708858057 2316 72-73 0.03866099647439114 2316 74-75 0.08922107851459415 2316 76-77 0.25094497963691964 2316 78-79 0.0911281126023269 2316 80-81 -0.2393331050047358 2316 82-83 0.2070928817346811 2316 84-85 0.4756964358591773 2316 86-87 0.4271948462929629 2316 88-89 0.4579478750985082 2316 90-91 0.7366265255099655 2316 92-93 0.9938313971174892 2316 94-95 1.1980165885846752 2316 96-97 1.2767522507045186 2316 98-99 1.0201124217531188 2316 100-101 0.6397108007311356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 28.0 4 43.0 5 115.0 6 214.0 7 593.0 8 1328.0 9 2449.0 10 3856.0 11 6292.0 12 9147.0 13 12594.0 14 17262.0 15 23024.0 16 29211.0 17 36091.0 18 37144.0 19 34281.0 20 36883.0 21 39912.0 22 44152.0 23 48675.0 24 55349.0 25 63065.0 26 72926.0 27 85673.0 28 99356.0 29 116674.0 30 134888.0 31 156523.0 32 183050.0 33 218277.0 34 262075.0 35 315462.0 36 361579.0 37 314000.0 38 98526.0 39 1751.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.22262760981573 19.46728647615579 15.109909855641707 23.200176058386766 2 15.784026964289563 20.91792612608728 39.116905143481354 24.181141766141803 3 19.725831088627555 24.10632651161772 29.657011694369622 26.510830705385104 4 13.311231040023653 17.1237226567098 36.51774935739333 33.04729694587322 5 13.84462352474608 37.41470807329297 33.96422811854248 14.776440283418474 6 32.26877793867657 36.258927373812305 17.071130193890653 14.401164493620469 7 29.01629168731129 32.22484253057001 20.255523406212276 18.503342375906424 8 26.369278852915123 35.22302546237445 19.422036640936142 18.985659043774287 9 26.15510325868633 16.90449093334191 20.118899586855758 36.821506221116 10-11 25.183509029342282 26.675276957315585 26.975673334081556 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22.67221134661059 26.251325518633223 28.63751569321673 22.438947441539455 38-39 22.26989107591833 26.646001354492803 28.611453232525484 22.47265433706338 40-41 22.59635942152778 26.54624829598663 28.449233619612713 22.40815866287288 42-43 22.35326847310995 26.512048325731254 28.268862353415614 22.865820847743183 44-45 22.436256503521868 26.590931254715645 28.229023201383768 22.743789040378722 46-47 22.71667848030578 26.913095243594505 27.840803813350316 22.529422462749395 48-49 22.555305919098625 26.751855963034032 27.880250007527874 22.812588110339465 50-51 22.46940261532018 27.01868486678401 27.88067772762237 22.63123479027344 52-53 22.8461213657479 27.09979770550411 27.37249492895056 22.68158599979743 54-55 22.407554289656154 26.86905126892576 27.69292571494269 23.030468726475394 56-57 22.599364955312655 26.908577209392803 27.566325283240573 22.925732552053972 58-59 22.878918942593803 26.656679687606932 27.474223876225334 22.99017749357393 60-61 22.463380316389685 26.877006862683373 27.947299409540964 22.712313411385978 62-63 22.3592903541933 26.95757221968251 27.802730017601533 22.88040740852265 64-65 22.171844364527267 27.04541021806896 27.648684161000798 23.134061256402973 66-67 22.235916962879116 26.902546218038825 27.477063780688272 23.38447303839379 68-69 22.45369328782575 26.704023533731274 27.605050362821387 23.237232815621585 70-71 22.40439682720547 26.818486821597205 27.411888774944217 23.36522757625311 72-73 22.5128709795084 26.682004170450575 27.475460792358238 23.32966405768279 74-75 22.232924729475595 27.12903665116381 27.24997878508331 23.388059834277282 76-77 22.240024883044217 26.727339047217562 27.51319773123575 23.519438338502475 78-79 22.40036859206863 26.862002441768475 27.46874905901579 23.2688799071471 80-81 22.35826201808744 27.025529774471597 27.369040571845048 23.24716763559592 82-83 22.298461865901643 26.930879947112636 27.409572900972716 23.361085286013008 84-85 22.30522585844345 26.841865725397334 27.379747766738127 23.47316064942109 86-87 22.207677196003345 26.977000988590323 27.25116456213388 23.564157253272462 88-89 22.108064679490514 27.03155068722643 26.9407200479594 23.91966458532366 90-91 22.144952143479056 26.797324900635033 27.221491951827133 23.836231004058774 92-93 22.27414777927994 26.69048911992768 27.254980661793365 23.780382438999016 94-95 22.01295856124418 26.567941940882534 27.25181563058229 24.167283867291 96-97 21.877968377455797 26.57418103578067 27.337746948473757 24.210103638289777 98-99 21.937643885039787 26.642205639609205 26.916493981807182 24.503656493543822 100-101 21.979986107641825 26.31231458321215 27.249022231194846 24.458677077951187 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 584.0 1 413.5 2 285.5 3 379.0 4 367.0 5 357.0 6 649.0 7 1440.0 8 2239.0 9 2470.0 10 2190.5 11 1881.0 12 1961.0 13 2114.0 14 2256.0 15 2514.5 16 2755.5 17 2919.0 18 3222.0 19 3546.0 20 3935.5 21 4622.0 22 5504.0 23 6588.5 24 8028.0 25 9975.0 26 12531.0 27 16318.0 28 20548.5 29 25369.5 30 30913.0 31 36093.5 32 42619.5 33 47856.0 34 51957.0 35 57628.5 36 64121.5 37 70518.5 38 75057.0 39 78792.0 40 83832.5 41 89699.5 42 94706.0 43 99906.5 44 104774.5 45 109581.5 46 113156.0 47 119583.0 48 129322.0 49 128970.0 50 120184.5 51 111889.5 52 105546.5 53 101357.0 54 98303.0 55 92313.5 56 82742.5 57 74940.5 58 68547.5 59 60492.0 60 50750.5 61 41293.5 62 33331.0 63 25267.5 64 18515.5 65 14338.0 66 10974.0 67 8347.0 68 6297.5 69 4560.0 70 3529.0 71 2761.0 72 2235.0 73 1955.5 74 1570.5 75 1266.0 76 910.5 77 582.0 78 376.5 79 225.5 80 132.5 81 67.0 82 35.5 83 24.0 84 14.0 85 9.0 86 11.0 87 8.5 88 4.5 89 2.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02443137179757411 2 0.013173778910456627 3 0.001368704302385104 4 0.0 5 3.079584680366484E-4 6 0.0 7 0.0 8 1.0265282267888281E-4 9 2.0530564535776563E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013858131061649178 26-27 0.0077673969160354656 28-29 2.0530564535776563E-4 30-31 0.0 32-33 3.592848793760898E-4 34-35 0.009666474135594797 36-37 0.0010607458343484557 38-39 0.011805074608071522 40-41 0.011172048868218412 42-43 0.004670703431889168 44-45 5.816993285136693E-4 46-47 8.5544018899069E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.71088037798138E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.84352151192552E-5 66-67 6.84352151192552E-4 68-69 9.752018154493866E-4 70-71 3.42176075596276E-5 72-73 2.0530564535776563E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.84352151192552E-5 82-83 0.0011976162645869661 84-85 0.006056516538054086 86-87 0.004414071375191961 88-89 0.0 90-91 0.0013173778910456626 92-93 6.501345436329244E-4 94-95 8.5544018899069E-5 96-97 0.0 98-99 0.0 100-101 6.84352151192552E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2922472.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.19126553006366 #Duplication Level Percentage of deduplicated Percentage of total 1 73.12131829037382 37.43172820510827 2 14.459595970972982 14.804100336150332 3 5.229585247871656 8.03127261107705 4 2.372955353156747 4.8589835029773205 5 1.290935200736963 3.3042303321515947 6 0.793168745709154 2.4361987123046904 7 0.5403008820106371 1.936108014199388 8 0.36153522268209626 1.4805956466231907 9 0.2751860817824858 1.2678411404434542 >10 1.4010359508665868 12.816102788405944 >50 0.09845386786486252 3.491215657732573 >100 0.050423425816735784 4.594993155474749 >500 0.003424314243039103 1.2040015378174351 >1k 0.0018128722461595045 1.4042502733253481 >5k 2.6857366609770437E-4 0.9383780862086728 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8767 0.2999857654752552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7100 0.242945013673356 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6286 0.2150918811198191 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5148 0.1761522437169629 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3415 0.11685312981612825 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.71088037798138E-5 0.0 16-17 0.0 0.0 0.0 3.42176075596276E-5 0.0 18-19 0.0 0.0 0.0 5.132641133944141E-5 0.0 20-21 0.0 0.0 0.0 1.1976162645869661E-4 0.0 22-23 0.0 0.0 0.0 2.9084966425683463E-4 0.0 24-25 0.0 0.0 0.0 6.330257398531107E-4 0.0 26-27 0.0 0.0 0.0 0.0012831602834860352 0.0 28-29 0.0 0.0 0.0 0.004157439318494754 0.0 30-31 0.0 0.0 0.0 0.013413302163374021 0.0 32-33 0.0 0.0 0.0 0.03320818813661859 3.42176075596276E-5 34-35 0.0 0.0 0.0 0.062087848916944285 3.42176075596276E-5 36-37 0.0 0.0 0.0 0.1106939604553953 3.42176075596276E-5 38-39 0.0 0.0 0.0 0.18583582665633752 3.42176075596276E-5 40-41 0.0 0.0 0.0 0.26612744279500367 3.42176075596276E-5 42-43 0.0 0.0 0.0 0.35052517184082516 3.42176075596276E-5 44-45 0.0 0.0 0.0 0.4571985634079642 3.42176075596276E-5 46-47 0.0 0.0 0.0 0.5809807587549171 3.42176075596276E-5 48-49 0.0 0.0 0.0 0.714035925750529 3.42176075596276E-5 50-51 0.0 0.0 0.0 0.8532331533030941 3.42176075596276E-5 52-53 0.0 0.0 0.0 0.994107043626081 3.42176075596276E-5 54-55 0.0 0.0 0.0 1.1499169196488452 3.42176075596276E-5 56-57 0.0 0.0 0.0 1.3356843110900636 3.42176075596276E-5 58-59 0.0 0.0 0.0 1.5309470886290786 3.42176075596276E-5 60-61 0.0 0.0 0.0 1.7424290121513568 5.132641133944141E-5 62-63 0.0 0.0 0.0 1.9582223542261483 8.5544018899069E-5 64-65 0.0 0.0 0.0 2.1849824395238002 1.0265282267888281E-4 66-67 0.0 0.0 0.0 2.4404853151715398 1.0265282267888281E-4 68-69 0.0 0.0 0.0 2.7273828457552374 1.0265282267888281E-4 70-71 0.0 0.0 0.0 3.0323301643266385 1.0265282267888281E-4 72-73 0.0 0.0 0.0 3.3406650260464428 1.0265282267888281E-4 74-75 0.0 0.0 0.0 3.6633541741375106 1.1976162645869661E-4 76-77 0.0 0.0 0.0 4.006317254707659 1.539792340183242E-4 78-79 0.0 0.0 0.0 4.389879526647304 1.71088037798138E-4 80-81 0.0 0.0 0.0 4.804716691896449 1.71088037798138E-4 82-83 0.0 0.0 0.0 5.224652280671979 1.71088037798138E-4 84-85 0.0 0.0 0.0 5.6501140130683885 1.71088037798138E-4 86-87 0.0 0.0 0.0 6.089382550115108 1.71088037798138E-4 88-89 0.0 0.0 0.0 6.558505949757603 1.71088037798138E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12475 0.0 23.99637 1 GTATCAA 16540 0.0 23.41357 1 TACATGG 11320 0.0 23.33216 2 GTACATG 11560 0.0 23.223953 1 GAGTACT 8950 0.0 22.44797 12-13 ACATGGG 11945 0.0 21.353188 3 GTACTTT 9490 0.0 21.19566 14-15 AGTACTT 9280 0.0 20.063091 12-13 TATCAAC 19470 0.0 19.884665 2 ATCAACG 19305 0.0 19.830782 3 GGACCGA 840 0.0 19.790462 6 TCAACGC 19510 0.0 19.598066 4 CAACGCA 19635 0.0 19.4733 5 AGAGTAC 15545 0.0 19.447662 10-11 AACGCAG 20045 0.0 19.122057 6 AGGACCG 1235 0.0 18.460732 5 ACTTTTT 11325 0.0 17.887125 16-17 TAAGGTG 1305 0.0 17.4705 5 ACGCAGA 22165 0.0 17.250248 7 CGCAGAG 22450 0.0 16.988943 8 >>END_MODULE