FastQCFastQC Report
Thu 26 May 2016
SRR938339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391805
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108120.45204354033878175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80910.3382800855420906No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA62440.2610580712056376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61860.2586331243558735No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA50320.21038504393125693No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA50030.20917257050637486No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA49760.20804371593838128No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA42690.1784844500283259No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG41210.17229665461858304No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35930.150221276400041No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34330.14353176784896762No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32640.1364659744418964No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG31870.13324664845169235No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT29060.12149819905886976No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA28480.11907325220910567No Hit
TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA26070.10899717995405145No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT25320.1058614728207358No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA24970.10439814282518851No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG24580.10276757511586437No Hit
GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT24530.10255852797364333No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT24170.10105338854965182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.061.950771
GTATCAA78800.046.0434271
TATCAAC103000.034.9022872
ATCAACG102900.034.6589363
TCAACGC105200.034.0367854
CAACGCA106900.033.4065445
AACGCAG110700.032.36636
TGGTATC15900.030.2060222
ACGCAGA124200.028.6581217
GTGGTAT17450.028.3404791
CGCAGAG126000.028.1343968
TAAGGTG17800.027.2489325
AGGTAAG15800.027.0866152
GTAAGGT18200.025.8662284
AAGGTAA16000.025.5592291
GCAGAGT140000.024.8447259
GGTAAGG18750.024.6002643
GTACATG79850.023.7611391
TACATGG78300.023.3205472
GAGTACT93450.022.6018312-13
Produced by FastQC (version 0.11.5)