##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2391805 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.033385246706985 33.0 31.0 34.0 31.0 34.0 2 32.14402888195317 33.0 31.0 34.0 31.0 34.0 3 32.19974830724077 34.0 31.0 34.0 31.0 34.0 4 35.84041508400559 37.0 35.0 37.0 35.0 37.0 5 35.828254811742596 37.0 35.0 37.0 35.0 37.0 6 35.8783333089445 37.0 35.0 37.0 35.0 37.0 7 35.83307794740792 37.0 35.0 37.0 35.0 37.0 8 35.80874402386482 37.0 35.0 37.0 35.0 37.0 9 37.465877444022404 39.0 37.0 39.0 35.0 39.0 10-11 37.34139823271546 39.0 37.0 39.0 35.0 39.0 12-13 37.24179479514426 39.0 37.0 39.0 34.0 39.0 14-15 38.41351824249887 40.0 38.0 41.0 34.0 41.0 16-17 38.25380099966343 40.0 38.0 41.0 34.0 41.0 18-19 38.40977295389884 40.0 38.0 41.0 34.0 41.0 20-21 38.39999122002003 40.0 38.0 41.0 34.0 41.0 22-23 38.334015105746495 40.0 38.0 41.0 34.0 41.0 24-25 38.21870721066308 40.0 38.0 41.0 34.0 41.0 26-27 38.03180025127466 40.0 38.0 41.0 33.5 41.0 28-29 37.84756303293956 40.0 38.0 41.0 33.0 41.0 30-31 37.59251799373277 40.0 37.0 41.0 33.0 41.0 32-33 37.315923747964405 39.0 36.5 41.0 32.5 41.0 34-35 37.08013926720615 39.0 36.0 41.0 31.5 41.0 36-37 36.81638365167729 39.0 36.0 40.0 31.0 41.0 38-39 36.68282322346512 39.0 36.0 40.0 31.0 41.0 40-41 36.57123344085325 39.0 35.5 40.0 30.5 41.0 42-43 36.273367394081035 39.0 35.0 40.0 30.0 41.0 44-45 35.936664569227005 38.5 35.0 40.0 29.5 41.0 46-47 35.53838669958462 38.0 35.0 40.0 28.0 41.0 48-49 35.144255698102484 38.0 34.5 40.0 26.5 41.0 50-51 34.42141562543769 37.5 33.5 39.5 25.5 40.5 52-53 34.42380357094328 38.0 34.0 39.5 26.0 40.5 54-55 34.515884656148806 38.0 34.0 40.0 26.0 41.0 56-57 34.191407326266145 37.5 34.0 40.0 24.5 41.0 58-59 33.82249200917299 37.0 33.0 40.0 23.0 41.0 60-61 33.356038222179485 36.5 33.0 39.5 22.0 41.0 62-63 32.814753502062246 36.0 32.0 39.0 20.5 40.5 64-65 32.3088627626416 35.0 31.5 39.0 18.5 40.0 66-67 31.790810496675107 35.0 31.0 38.0 15.5 40.0 68-69 31.22080081779242 35.0 30.5 37.5 10.0 40.0 70-71 30.5960053181593 34.5 30.0 37.0 7.0 39.0 72-73 30.005857500925032 34.0 29.0 36.0 2.0 39.0 74-75 29.38716575975048 34.0 29.0 36.0 2.0 38.5 76-77 28.69642550291516 34.0 27.5 35.0 2.0 37.5 78-79 27.971496004063876 33.0 26.0 35.0 2.0 37.0 80-81 27.33939535204584 33.0 25.5 35.0 2.0 36.0 82-83 26.64289208359377 32.0 24.5 35.0 2.0 36.0 84-85 25.999513965394335 32.0 23.5 34.5 2.0 35.0 86-87 25.29297476173852 31.0 20.0 34.0 2.0 35.0 88-89 24.493801543186002 31.0 17.5 34.0 2.0 35.0 90-91 23.597115776578775 30.5 8.5 34.0 2.0 35.0 92-93 22.68157103108322 29.5 2.0 34.0 2.0 35.0 94-95 21.686329571181595 29.0 2.0 34.0 2.0 35.0 96-97 20.407261043437906 28.0 2.0 34.0 2.0 35.0 98-99 18.80435466101961 26.0 2.0 33.0 2.0 35.0 100-101 14.779050131595177 10.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29358640603258834 1101 2 0.24958656426652936 1101 3 0.25918943136636585 1101 4 0.08584616949384838 1101 5 0.09138103209582482 1101 6 0.2175355120115796 1101 7 0.3051570592498436 1101 8 0.33241417287079855 1101 9 0.43571751778193857 1101 10-11 0.3777760647248769 1101 12-13 0.42882262644459956 1101 14-15 0.5574036190946501 1101 16-17 0.4589910873007028 1101 18-19 0.4982298250232233 1101 20-21 0.5708326686247389 1101 22-23 0.48540126994326016 1101 24-25 0.4628031078529986 1101 26-27 0.42258283517660544 1101 28-29 0.472908257112195 1101 30-31 0.34951881277858377 1101 32-33 0.5093548918682274 1101 34-35 0.46886478521408037 1101 36-37 0.4504279113517313 1101 38-39 0.5446838674138519 1101 40-41 0.6317277486627049 1101 42-43 0.7227604791951734 1101 44-45 0.7006316561565384 1101 46-47 0.7653741377166838 1101 48-49 0.6493966584126483 1101 50-51 0.7878823467623164 1101 52-53 0.6510168508620708 1101 54-55 0.600417580411829 1101 56-57 0.6151422036539884 1101 58-59 0.5821937397172334 1101 60-61 0.6513801007849125 1101 62-63 0.6211414661439889 1101 64-65 0.45750759022663345 1101 66-67 0.6789395575420443 1101 68-69 0.7159019539984612 1101 70-71 0.8084533414560404 1101 72-73 0.7891449436940832 1101 74-75 0.5834073090691874 1101 76-77 0.7047165032423557 1101 78-79 0.7837532670234211 1101 80-81 0.7904952522335087 1101 82-83 0.8628621300277679 1101 84-85 0.8390846962240097 1101 86-87 1.0334871302908653 1101 88-89 0.9211595205330845 1101 90-91 0.7171356875008783 1101 92-93 0.692958014447143 1101 94-95 0.8732896982752933 1101 96-97 0.9233010610738894 1101 98-99 0.9352095822191302 1101 100-101 1.1236568699243161 1102 1 0.28366064797836543 1102 2 0.2112162169128382 1102 3 0.16357414751152533 1102 4 0.07914052707219099 1102 5 0.13183329495813467 1102 6 0.1970296152680575 1102 7 0.15323014047916672 1102 8 0.20280655920136326 1102 9 0.3860825355219859 1102 10-11 0.44748336378498976 1102 12-13 0.3249622873150102 1102 14-15 0.38928915286378896 1102 16-17 0.4452769579140323 1102 18-19 0.2817132859396665 1102 20-21 0.2811421611124487 1102 22-23 0.40040271380247106 1102 24-25 0.26930824241899387 1102 26-27 0.26754528639359165 1102 28-29 0.2411612550198683 1102 30-31 0.3389666787659351 1102 32-33 0.2377724334347633 1102 34-35 0.1513899795721585 1102 36-37 0.3334937507686959 1102 38-39 0.32250925783281303 1102 40-41 0.4553914471150975 1102 42-43 0.4606358614801209 1102 44-45 0.5046647799191959 1102 46-47 0.3694217845076295 1102 48-49 0.45462638401185274 1102 50-51 0.46479394036069976 1102 52-53 0.36254869823066826 1102 54-55 0.29793737929603026 1102 56-57 0.34126687991147975 1102 58-59 0.3489295596406876 1102 60-61 0.2556757085820962 1102 62-63 0.3033618457804508 1102 64-65 0.5111130445807035 1102 66-67 0.3484562277831742 1102 68-69 0.4718775046492141 1102 70-71 0.5665686464199311 1102 72-73 0.523346365712456 1102 74-75 0.3614121264364769 1102 76-77 0.6208263130976377 1102 78-79 0.7979053388910202 1102 80-81 1.099423846781523 1102 82-83 1.003902969571385 1102 84-85 0.843814672035851 1102 86-87 0.9855321471752951 1102 88-89 0.9947339980558816 1102 90-91 0.8896799588135593 1102 92-93 1.0639383582601347 1102 94-95 1.1745420561564863 1102 96-97 1.46537463991395 1102 98-99 1.7085691414395896 1102 100-101 1.559067953246533 1103 1 0.19263784963848707 1103 2 0.06884703282350557 1103 3 0.10142405968749557 1103 4 0.11595181163700374 1103 5 0.07463097652346562 1103 6 0.12722222967119734 1103 7 0.04659800496384747 1103 8 0.18112434753199125 1103 9 0.17477522922109756 1103 10-11 0.24378473036876613 1103 12-13 0.20518123396526988 1103 14-15 0.3371767381069546 1103 16-17 0.32458250474204675 1103 18-19 0.3170703123108183 1103 20-21 0.3011090363424884 1103 22-23 0.25856765540771676 1103 24-25 0.3460091410069239 1103 26-27 0.2721302393097389 1103 28-29 0.2961380497270838 1103 30-31 0.3075243299294854 1103 32-33 0.3147441352460092 1103 34-35 0.3027353180163459 1103 36-37 0.2983618950335014 1103 38-39 0.3743254348094567 1103 40-41 0.38140980188826035 1103 42-43 0.4071267903202127 1103 44-45 0.36260230666288606 1103 46-47 0.18480879373289127 1103 48-49 0.2556264109425612 1103 50-51 0.44155722842052825 1103 52-53 0.4200307967910746 1103 54-55 0.35577256935201973 1103 56-57 0.2535775046739701 1103 58-59 0.29652539555488033 1103 60-61 0.458531261245092 1103 62-63 0.5384033340311802 1103 64-65 0.7039518702821681 1103 66-67 0.6515186980471839 1103 68-69 0.7927995222978055 1103 70-71 0.6309315406929699 1103 72-73 0.7930723607272832 1103 74-75 0.7211629874710326 1103 76-77 0.7664683261979306 1103 78-79 0.6225961594098237 1103 80-81 0.8774796591762275 1103 82-83 0.9376033085990656 1103 84-85 0.9007162642391116 1103 86-87 0.8414199478641216 1103 88-89 0.949352015895979 1103 90-91 0.8547246488261564 1103 92-93 0.8749483794755513 1103 94-95 0.988403740043843 1103 96-97 1.3348070902193143 1103 98-99 1.4177279757588863 1103 100-101 1.5240812241006267 1104 1 0.1909255338259257 1104 2 0.1130375491608504 1104 3 0.15653418955989906 1104 4 0.04350761984648699 1104 5 0.06387103401565497 1104 6 0.1753705434827637 1104 7 0.11958730806684059 1104 8 0.18641446235576353 1104 9 0.2153068501235893 1104 10-11 0.2190436847876711 1104 12-13 0.25907292657239367 1104 14-15 0.2728746405234048 1104 16-17 0.43545505315137234 1104 18-19 0.45524892910844983 1104 20-21 0.43939708685746126 1104 22-23 0.438660845949407 1104 24-25 0.45788165742894904 1104 26-27 0.5241148121419528 1104 28-29 0.48680662536728647 1104 30-31 0.4431946648709939 1104 32-33 0.4875954549741621 1104 34-35 0.44620537691146467 1104 36-37 0.5632623674234907 1104 38-39 0.686278888382283 1104 40-41 0.6446992294497207 1104 42-43 0.7658931212618469 1104 44-45 0.5416968564655775 1104 46-47 0.3676428044846105 1104 48-49 0.3090585350277877 1104 50-51 0.44743539186126924 1104 52-53 0.5521688582096047 1104 54-55 0.5828523110273878 1104 56-57 0.5626914552364752 1104 58-59 0.6587318208603605 1104 60-61 0.604710949418056 1104 62-63 0.7685855116387259 1104 64-65 0.6563999669119411 1104 66-67 0.6612677225341912 1104 68-69 0.7531719075188263 1104 70-71 0.8713438131435041 1104 72-73 0.8693992263000716 1104 74-75 0.8053254133875924 1104 76-77 0.7776969954162389 1104 78-79 0.8847785079702177 1104 80-81 0.9311945589252559 1104 82-83 1.0284702585678822 1104 84-85 1.0933084576664243 1104 86-87 1.3084662634783975 1104 88-89 1.1892135361207785 1104 90-91 1.2953046621280961 1104 92-93 1.4642089300738128 1104 94-95 1.2750917421929664 1104 96-97 1.507833211895008 1104 98-99 1.7306838528007802 1104 100-101 1.327879244365704 1105 1 0.29070478270542566 1105 2 0.13706670788528186 1105 3 0.1407167406907064 1105 4 0.05688495743857658 1105 5 0.05896453769913279 1105 6 0.16035212609056515 1105 7 0.14730046040314448 1105 8 0.19736521193074452 1105 9 0.1539570999819162 1105 10-11 0.24437545543317896 1105 12-13 0.35092522681453175 1105 14-15 0.40575322740713204 1105 16-17 0.3329678036536592 1105 18-19 0.2502721762363649 1105 20-21 0.3404441091644017 1105 22-23 0.2960393941952475 1105 24-25 0.33248214169044843 1105 26-27 0.4037948904480686 1105 28-29 0.38049448276772324 1105 30-31 0.39580716551495954 1105 32-33 0.4705782353564203 1105 34-35 0.43081519866201745 1105 36-37 0.46637992963048447 1105 38-39 0.4703419840496892 1105 40-41 0.5998309202435976 1105 42-43 0.6997881421263017 1105 44-45 0.7399092102855818 1105 46-47 0.6581786475632683 1105 48-49 0.5916492696627884 1105 50-51 0.5587027145133803 1105 52-53 0.6285815881050212 1105 54-55 0.5690247776700161 1105 56-57 0.7180950923494436 1105 58-59 0.8520328486281201 1105 60-61 0.7870361773815873 1105 62-63 0.8901029502044864 1105 64-65 1.006247798038622 1105 66-67 1.0330194998769358 1105 68-69 1.0062392404743647 1105 70-71 1.060810674716837 1105 72-73 1.1108294720370253 1105 74-75 1.1220988279463455 1105 76-77 1.266059393025838 1105 78-79 1.2578042408021162 1105 80-81 1.333040677105668 1105 82-83 1.2591042418763152 1105 84-85 1.244433270916641 1105 86-87 1.5249812549780621 1105 88-89 1.4569590798846228 1105 90-91 1.2705885217952968 1105 92-93 1.4995824296359181 1105 94-95 1.5076149893208814 1105 96-97 1.7198327224817866 1105 98-99 1.9435609585655556 1105 100-101 1.3164367632982685 1106 1 0.0870143065149449 1106 2 0.001709565028143345 1106 3 -0.00833087835691515 1106 4 -0.017866124812506712 1106 5 0.16157276280736 1106 6 -0.03137947564103172 1106 7 0.1650926681953493 1106 8 0.10563361019914908 1106 9 -0.009473635948815229 1106 10-11 -0.12801090820318706 1106 12-13 0.0536254432647425 1106 14-15 0.09915149580540117 1106 16-17 0.0356409638268218 1106 18-19 -0.10839665493246997 1106 20-21 0.0538694338397292 1106 22-23 0.013355411511263071 1106 24-25 -0.11170616999786631 1106 26-27 -0.041448616045435926 1106 28-29 0.14183106285430114 1106 30-31 -0.06782378686599344 1106 32-33 0.10754793232612059 1106 34-35 -0.07502354592672589 1106 36-37 0.09020568720624311 1106 38-39 0.016559516517226314 1106 40-41 0.23381901548169282 1106 42-43 -0.11936229077412719 1106 44-45 0.1309535825799486 1106 46-47 0.14993730557193174 1106 48-49 0.34216333892685924 1106 50-51 0.05847003053069244 1106 52-53 0.023505830529266802 1106 54-55 0.2074717473669878 1106 56-57 -0.48410728860292807 1106 58-59 -0.14792927258400113 1106 60-61 0.32898964058504987 1106 62-63 0.3241397467412881 1106 64-65 0.34381814435896985 1106 66-67 0.0931439587513978 1106 68-69 0.03980525779038402 1106 70-71 -0.08882677117060922 1106 72-73 0.2998338010413555 1106 74-75 0.5996096504571682 1106 76-77 0.6695929428093912 1106 78-79 0.8037349767328514 1106 80-81 0.5987170840320779 1106 82-83 0.2735306488027227 1106 84-85 0.4204776432110151 1106 86-87 0.29284922034602445 1106 88-89 0.42572531365085453 1106 90-91 0.5225744525312237 1106 92-93 0.4203454166489031 1106 94-95 0.4873931278490211 1106 96-97 0.44227319867226456 1106 98-99 0.5920133395179334 1106 100-101 0.40541403602554027 1107 1 0.0807263149506241 1107 2 -0.018931484469440818 1107 3 -0.009603983430729102 1107 4 0.009637230286678289 1107 5 -0.05685448510909197 1107 6 0.10218462648931137 1107 7 0.07303494921169573 1107 8 0.04011416190150641 1107 9 0.20214360644158802 1107 10-11 0.22608181940787375 1107 12-13 0.2137858254641074 1107 14-15 0.31070198863845633 1107 16-17 0.40743853574999633 1107 18-19 0.3508767405406914 1107 20-21 0.32767090218901984 1107 22-23 0.3451688003306046 1107 24-25 0.29343009213970817 1107 26-27 0.2521956560390137 1107 28-29 0.34837555034175693 1107 30-31 0.34516312542776717 1107 32-33 0.3582173310021233 1107 34-35 0.1882204966576566 1107 36-37 0.22203493521291762 1107 38-39 0.3664275680786133 1107 40-41 0.35286596995384656 1107 42-43 0.37992059201731365 1107 44-45 0.38715255123938164 1107 46-47 0.33390655707467687 1107 48-49 0.28387074608365026 1107 50-51 0.3337098919284429 1107 52-53 0.3239825929431319 1107 54-55 0.445106374246123 1107 56-57 0.4512675098091776 1107 58-59 0.44360290817408554 1107 60-61 0.44676370143921673 1107 62-63 0.5558892758996237 1107 64-65 0.5668555523051992 1107 66-67 0.5142039727356718 1107 68-69 0.6703091006476853 1107 70-71 0.8036155154845588 1107 72-73 0.8374152820216096 1107 74-75 0.6511601150961184 1107 76-77 0.7789283653263332 1107 78-79 0.7923224235869526 1107 80-81 0.7171937421277335 1107 82-83 0.6712266965522709 1107 84-85 0.8752647212861469 1107 86-87 1.071146521605737 1107 88-89 1.0717463492769355 1107 90-91 0.8905595463048357 1107 92-93 0.9554777457080057 1107 94-95 1.2909279644923437 1107 96-97 1.1736937730426895 1107 98-99 1.2596600334765 1107 100-101 1.1025811622562145 1108 1 0.09651777357785107 1108 2 0.06146067944121825 1108 3 0.026469765526194067 1108 4 -0.049125974423027685 1108 5 -0.019630240479592942 1108 6 0.042812856296464474 1108 7 0.03274091890452269 1108 8 0.10655328240410711 1108 9 -0.06391384037044645 1108 10-11 0.140185262268254 1108 12-13 0.0998755558317228 1108 14-15 0.1652668657004881 1108 16-17 0.3176589520298023 1108 18-19 0.2172491981000917 1108 20-21 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-2.950386153368399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6566.0 3 1435.0 4 1296.0 5 1338.0 6 1598.0 7 1953.0 8 2351.0 9 3094.0 10 4196.0 11 5571.0 12 7992.0 13 10255.0 14 12877.0 15 16381.0 16 20988.0 17 26351.0 18 23951.0 19 20834.0 20 20826.0 21 22166.0 22 23945.0 23 27635.0 24 31595.0 25 35849.0 26 42110.0 27 50336.0 28 59691.0 29 72461.0 30 87829.0 31 109014.0 32 138573.0 33 176943.0 34 230102.0 35 296491.0 36 347536.0 37 315484.0 38 128097.0 39 6095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97665861791422 17.969749234323228 14.776965933916605 24.276626213845944 2 15.669212352371781 19.42764548554048 39.563588366429755 25.33955379565798 3 19.266096130634004 22.855593111380085 29.483972926324174 28.394337831661737 4 13.306792351275142 15.830543419073932 35.408270590781 35.454393638869924 5 14.369300475995692 36.48884409958354 33.61654148874571 15.525313935675058 6 33.79038214095174 34.93912734196019 16.447020179301834 14.82347033778624 7 29.565460306627138 32.329462124928334 19.644427605224564 18.460649963219964 8 27.5903556515539 33.56991859888886 19.195021840087488 19.644703909469754 9 26.808819378378097 16.285322990392356 19.393432511264674 37.512425119964874 10-11 25.526196357126274 25.625037907386766 26.67805805424782 22.170707681239136 12-13 26.595079411490353 23.04447453390272 27.062316550861294 23.298129503745635 14-15 22.990701536445172 24.533524255484114 25.375695021937716 27.100079186132998 16-17 22.721012786551494 27.566092845737494 26.511789941558426 23.201104426152583 18-19 22.897819117902305 25.555613643635205 29.001256793691354 22.545310444771133 20-21 22.842736419925057 24.966556485030107 29.529640700778632 22.661066394266204 22-23 22.66152322255398 24.82840258463085 29.63221014961726 22.877864043197903 24-25 22.57384354512594 25.167684597360378 29.5348247739876 22.723647083526085 26-27 21.944958672258323 25.59674188603587 29.086807395092123 23.371492046613685 28-29 22.22406271738235 26.294024957950963 28.921959240561502 22.559953084105192 30-31 22.174502821224813 25.605279533961898 28.77714154233929 23.443076102474 32-33 21.729797198152177 25.67977770601847 28.841121542237698 23.749303553591655 34-35 22.989867002446186 25.688220769455217 28.59063589292008 22.731276335178517 36-37 23.169125373114348 25.086042176980015 28.569746449557616 23.175086000348017 38-39 22.373602815603757 25.52170995525706 28.55169171890719 23.55299551023199 40-41 22.910799202010864 25.436886504029676 28.820205687136397 22.832108606823056 42-43 22.59743524008396 25.649251119045157 28.367664101181838 23.385649539689037 44-45 22.384568410590862 25.938326241692245 28.823282352820662 22.853822994896234 46-47 23.08474224752233 26.424887040163775 27.664601903863506 22.82576880845039 48-49 22.547896617189366 26.59742673998609 27.426043809769507 23.428632833055037 50-51 22.87650990940439 26.650609190351133 27.630218383629153 22.842662516615327 52-53 23.43042183510774 25.98325471086696 27.75108943894417 22.83523401508113 54-55 22.48866177714844 26.562979220627714 27.98869031478582 22.959668687438025 56-57 22.661805299876626 26.70306210494339 27.550414309629257 23.084718285550732 58-59 22.562417019497154 26.0988343526214 27.63006638440228 23.70868224347917 60-61 21.97912236483839 26.49346559794452 28.112481793124733 23.414930244092357 62-63 22.38084157528858 26.490790899468113 28.23915792713127 22.88920959811204 64-65 22.011920394394497 26.660012865780473 27.906826996229118 23.42123974359591 66-67 21.92035280191437 26.597478588574912 28.027798997908093 23.454369611602623 68-69 22.877012243539433 25.971055555253564 28.151234326567696 23.000697874639304 70-71 22.425256776432462 26.423125572295852 27.52452651699307 23.627091134278615 72-73 22.305118612816262 26.6643651766296 27.54739295614407 23.483123254410067 74-75 22.34869442628737 27.105401495752503 27.47480209487318 23.071101983086947 76-77 22.564043375406523 26.563589230835493 27.534375524033074 23.33799186972491 78-79 22.667939953435 26.970300126206748 27.365791135674655 22.995968784683598 80-81 23.035166786036974 26.981076774551504 26.969996868358585 23.01375957105293 82-83 22.691915631629694 27.070073947339594 27.204643387846815 23.0333670331839 84-85 22.47617084491325 27.335428623752094 27.20100614078696 22.987394390547696 86-87 23.00978538389296 27.299954087891997 26.55170798895768 23.13855253925736 88-89 22.65359289601488 27.400380427236183 26.363302155252455 23.58272452149648 90-91 22.529093961387613 27.422454440441545 26.497085376023715 23.551366222147127 92-93 22.83771240426478 26.84500508224964 26.81775895478586 23.49952355869972 94-95 22.495404248377632 26.49643737752505 27.254825277784168 23.75333309631315 96-97 23.066288088312454 25.949479991526115 27.107926409353567 23.876305510807867 98-99 22.647186187466506 26.46652993195814 26.590314641075572 24.295969239499783 100-101 23.163060497252882 25.73868860037674 27.05768162720572 24.040569275164657 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 840.0 1 602.0 2 510.5 3 677.0 4 619.0 5 620.5 6 1066.0 7 2455.5 8 3914.5 9 4190.5 10 3410.5 11 2561.0 12 2353.5 13 2496.5 14 2735.5 15 2865.5 16 3002.5 17 3318.0 18 3584.5 19 3943.5 20 4602.0 21 5486.5 22 6617.0 23 7993.5 24 9790.0 25 12155.0 26 14916.0 27 18217.5 28 21175.0 29 23990.5 30 26973.0 31 32184.5 32 40943.0 33 42020.5 34 37923.5 35 38800.5 36 42087.0 37 45935.5 38 49581.0 39 52500.0 40 55973.0 41 62094.0 42 66187.5 43 68858.0 44 71902.5 45 78178.5 46 82602.0 47 83596.5 48 84900.5 49 83530.5 50 82961.5 51 83018.5 52 83286.0 53 86897.0 54 90544.0 55 87712.0 56 82607.5 57 77506.0 58 74200.5 59 66808.0 60 53355.5 61 44712.0 62 39255.0 63 30065.5 64 21184.0 65 16458.0 66 12715.5 67 9644.0 68 7437.5 69 5861.5 70 4636.0 71 3469.5 72 2593.5 73 2071.0 74 1610.5 75 1211.5 76 936.5 77 677.5 78 444.5 79 297.5 80 191.0 81 117.0 82 75.5 83 45.0 84 34.0 85 25.5 86 18.0 87 12.0 88 8.5 89 6.5 90 7.0 91 8.0 92 5.0 93 3.0 94 3.5 95 3.5 96 1.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005435225697747099 2 0.0949910214252416 3 0.10339471654252752 4 0.1063631859620663 5 0.13955987214676782 6 0.08278266831953274 7 0.023496898785645152 8 0.023413279928756733 9 0.020068525653220058 10-11 0.046408465573071385 12-13 0.04833169928150497 14-15 0.025231990066079804 16-17 0.02922479048250171 18-19 0.033447542755366766 20-21 0.05046398013215961 22-23 0.028033221771841768 24-25 0.04210209444331791 26-27 0.05098659798771221 28-29 0.04782998614017447 30-31 0.0603101005307707 32-33 0.045864943003296674 34-35 0.056338204828570895 36-37 0.046701131572180844 38-39 0.02932931405361223 40-41 0.033238495613145715 42-43 0.02943383762472275 44-45 0.00583241526796708 46-47 0.010326928825719488 48-49 0.007086698121293333 50-51 0.00786017254751119 52-53 0.00865455168795115 54-55 0.009469835542613214 56-57 0.012396495533707806 58-59 0.017978054231009635 60-61 0.022493472502984148 62-63 0.00963707325639005 64-65 0.007922886690177502 66-67 0.008696361116395358 68-69 0.010786832538605781 70-71 0.02794960291495335 72-73 0.025106561780747175 74-75 0.026507177633628157 76-77 0.020737476508327395 78-79 0.0039509909879776985 80-81 0.0036792297030903438 82-83 0.004306371129753471 84-85 0.003846467416867178 86-87 0.01166483053593416 88-89 0.03382382761136464 90-91 0.03173335618915422 92-93 0.026737129490071305 94-95 0.04222752272865054 96-97 0.038903673167335964 98-99 0.023266946929202004 100-101 0.0657453262285178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2391805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.33716851393997 #Duplication Level Percentage of deduplicated Percentage of total 1 72.01954454496415 33.37181771877209 2 14.529381904209934 13.465008353975314 3 5.362772767937982 7.454871163497318 4 2.5789507377592233 4.780050996987958 5 1.4644812432937468 3.392995707800334 6 0.9245214009145803 2.570382236935366 7 0.5994598982750008 1.944409202660262 8 0.4120926228746665 1.527616424759595 9 0.3259255533195835 1.35922205584518 >10 1.6062520971551901 13.281184824280908 >50 0.10839272510023566 3.46131168255246 >100 0.056755397594365244 5.0191824633594715 >500 0.005689399337545243 1.7861604625667191 >1k 0.005237859707581076 4.854695758217247 >5k 4.515396299638859E-4 1.2786505269934074 >10k+ 9.030792599277717E-5 0.452440420796332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10812 0.45204354033878175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8091 0.3382800855420906 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6244 0.2610580712056376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6186 0.2586331243558735 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5032 0.21038504393125693 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5003 0.20917257050637486 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4976 0.20804371593838128 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4269 0.1784844500283259 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4121 0.17229665461858304 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3593 0.150221276400041 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3433 0.14353176784896762 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3264 0.1364659744418964 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3187 0.13324664845169235 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2906 0.12149819905886976 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2848 0.11907325220910567 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 2607 0.10899717995405145 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2532 0.1058614728207358 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2497 0.10439814282518851 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 2458 0.10276757511586437 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 2453 0.10255852797364333 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 2417 0.10105338854965182 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.1809428444208455E-5 2 0.0 0.0 0.0 0.0 4.1809428444208455E-5 3 0.0 0.0 0.0 0.0 4.1809428444208455E-5 4 0.0 0.0 0.0 0.0 4.1809428444208455E-5 5 0.0 0.0 0.0 0.0 4.1809428444208455E-5 6 0.0 0.0 0.0 0.0 4.1809428444208455E-5 7 0.0 0.0 0.0 0.0 4.1809428444208455E-5 8 0.0 0.0 0.0 0.0 4.1809428444208455E-5 9 0.0 0.0 0.0 0.0 4.1809428444208455E-5 10-11 4.1809428444208455E-5 0.0 0.0 4.1809428444208455E-5 4.1809428444208455E-5 12-13 4.1809428444208455E-5 0.0 0.0 4.1809428444208455E-5 4.180942844420845E-4 14-15 4.1809428444208455E-5 0.0 0.0 4.1809428444208455E-5 6.062367124410226E-4 16-17 4.1809428444208455E-5 0.0 0.0 4.1809428444208455E-5 7.525697119957522E-4 18-19 4.1809428444208455E-5 0.0 0.0 4.1809428444208455E-5 7.525697119957522E-4 20-21 4.1809428444208455E-5 0.0 0.0 4.1809428444208455E-5 9.407121399946901E-4 22-23 4.1809428444208455E-5 0.0 0.0 1.463329995547296E-4 0.0010034262826610029 24-25 4.1809428444208455E-5 2.0904714222104227E-5 0.0 3.3447542755366764E-4 0.0010452357111052114 26-27 4.1809428444208455E-5 4.1809428444208455E-5 0.0 6.062367124410226E-4 0.001212473424882045 28-29 4.1809428444208455E-5 4.1809428444208455E-5 0.0 0.001672377137768338 0.001212473424882045 30-31 4.1809428444208455E-5 4.1809428444208455E-5 0.0 0.005414320983524994 0.001212473424882045 32-33 8.361885688841691E-5 4.1809428444208455E-5 0.0 0.014445157527474021 0.001296092281770462 34-35 8.361885688841691E-5 4.1809428444208455E-5 0.0 0.02928750462516802 0.001379711138658879 36-37 8.361885688841691E-5 4.1809428444208455E-5 0.0 0.05132107341526587 0.001379711138658879 38-39 8.361885688841691E-5 4.1809428444208455E-5 0.0 0.0880088468750588 0.0014215205671030875 40-41 1.2542828533262536E-4 4.1809428444208455E-5 0.0 0.12622266447306532 0.0014215205671030875 42-43 1.2542828533262536E-4 4.1809428444208455E-5 0.0 0.16803209291727378 0.0014215205671030875 44-45 1.2542828533262536E-4 4.1809428444208455E-5 0.0 0.2260217701693909 0.0014633299955472958 46-47 1.2542828533262536E-4 4.1809428444208455E-5 0.0 0.2961152769561064 0.0015260441382136086 48-49 1.2542828533262536E-4 4.1809428444208455E-5 0.0 0.37112139158501634 0.0016305677093241297 50-51 1.463329995547296E-4 4.1809428444208455E-5 0.0 0.4575205754649731 0.001755995994656755 52-53 1.6723771377683382E-4 4.1809428444208455E-5 0.0 0.5390489609311796 0.0018814242799893805 54-55 1.6723771377683382E-4 4.1809428444208455E-5 0.0 0.633391936215536 0.0019232337084335888 56-57 1.6723771377683382E-4 4.1809428444208455E-5 0.0 0.7460474411584557 0.0019859478510999016 58-59 1.6723771377683382E-4 4.1809428444208455E-5 0.0 0.8638664105142351 0.0020068525653220057 60-61 1.6723771377683382E-4 4.1809428444208455E-5 0.0 0.9986809125325853 0.00202775727954411 62-63 1.6723771377683382E-4 4.1809428444208455E-5 0.0 1.1354604576878131 0.002090471422210423 64-65 1.6723771377683382E-4 4.1809428444208455E-5 0.0 1.2827759788109816 0.002090471422210423 66-67 1.6723771377683382E-4 4.1809428444208455E-5 0.0 1.4622011409793023 0.002090471422210423 68-69 2.0904714222104225E-4 4.1809428444208455E-5 0.0 1.6607541166608482 0.002111376136432527 70-71 2.0904714222104225E-4 4.1809428444208455E-5 0.0 1.8613766590503826 0.0021531855648767354 72-73 2.0904714222104225E-4 4.1809428444208455E-5 0.0 2.0848271493704544 0.002404042135541986 74-75 2.0904714222104225E-4 4.1809428444208455E-5 0.0 2.3141100549584936 0.0026967081346514453 76-77 2.0904714222104225E-4 4.1809428444208455E-5 0.0 2.566325432048181 0.002759422277317758 78-79 2.508565706652507E-4 4.1809428444208455E-5 0.0 2.854287870457667 0.002759422277317758 80-81 2.508565706652507E-4 4.1809428444208455E-5 0.0 3.1589322708163916 0.002780326991539862 82-83 2.508565706652507E-4 4.1809428444208455E-5 0.0 3.4811575358359064 0.0030938977048714255 84-85 2.508565706652507E-4 4.1809428444208455E-5 0.0 3.8246010857908566 0.0030938977048714255 86-87 2.508565706652507E-4 4.1809428444208455E-5 0.0 4.179061420140856 0.003156611847537738 88-89 2.508565706652507E-4 4.1809428444208455E-5 0.0 4.5401067394708186 0.003219325990204051 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3930 0.0 61.95077 1 GTATCAA 7880 0.0 46.043427 1 TATCAAC 10300 0.0 34.902287 2 ATCAACG 10290 0.0 34.658936 3 TCAACGC 10520 0.0 34.036785 4 CAACGCA 10690 0.0 33.406544 5 AACGCAG 11070 0.0 32.3663 6 TGGTATC 1590 0.0 30.206022 2 ACGCAGA 12420 0.0 28.658121 7 GTGGTAT 1745 0.0 28.340479 1 CGCAGAG 12600 0.0 28.134396 8 TAAGGTG 1780 0.0 27.248932 5 AGGTAAG 1580 0.0 27.086615 2 GTAAGGT 1820 0.0 25.866228 4 AAGGTAA 1600 0.0 25.559229 1 GCAGAGT 14000 0.0 24.844725 9 GGTAAGG 1875 0.0 24.600264 3 GTACATG 7985 0.0 23.761139 1 TACATGG 7830 0.0 23.320547 2 GAGTACT 9345 0.0 22.60183 12-13 >>END_MODULE