FastQCFastQC Report
Thu 26 May 2016
SRR938339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391805
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116100.4854074642372601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97370.4070984047612577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.3029093090782903No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51000.21322808506546312No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44120.1844631982958477No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42120.176101312607006No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA40800.17058246805237048No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40770.17045703976703788No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35870.14997041982937573No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34690.14503690727295912No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33000.1379711138658879No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA31350.1310725581725935No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27550.1151849753637943No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA26330.11008422509360086No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA25810.10791013481450203No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG25010.10456538053896536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.064.179261
GTATCAA89650.043.6806531
GTGGTAT18000.033.794781
TGGTATC17250.032.7839282
AGGTAAG17450.031.5911672
TATCAAC125800.030.9012322
ATCAACG129000.030.085893
TCAACGC130650.029.742294
CAACGCA134100.028.9771065
AAGGTAA18300.028.5662861
AACGCAG139250.027.996646
TAAGGTG22350.027.8435295
GTAAGGT22100.027.5136494
GTGTAAG8300.026.3385541
GGTAAGG23050.024.5248583
ACGCAGA159250.024.480587
CGCAGAG163350.023.8079918
TAAGGTA9300.022.9858254
GTCGTAA1703.1376094E-622.3641971
AGAGTAC158550.021.75841910-11
Produced by FastQC (version 0.11.5)