##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938339_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2391805 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.168606136369814 31.0 31.0 33.0 30.0 34.0 2 31.430377894518994 31.0 31.0 34.0 30.0 34.0 3 31.445683071989563 31.0 31.0 34.0 28.0 34.0 4 35.24727183027044 37.0 35.0 37.0 33.0 37.0 5 35.119043985609196 37.0 35.0 37.0 32.0 37.0 6 35.1566799969061 37.0 35.0 37.0 32.0 37.0 7 35.086768779227405 37.0 35.0 37.0 32.0 37.0 8 35.05493173565571 37.0 35.0 37.0 32.0 37.0 9 36.521367753642124 38.0 35.0 39.0 32.0 39.0 10-11 36.35924855914257 38.0 35.0 39.0 32.0 39.0 12-13 36.21764566927488 38.0 35.0 39.0 32.0 39.0 14-15 36.98401374694007 38.5 36.0 40.0 31.0 41.0 16-17 36.81813400339911 38.0 36.0 40.0 31.0 41.0 18-19 37.126271372457204 38.5 36.0 40.0 31.0 41.0 20-21 37.067905410349084 39.0 36.0 40.0 31.0 41.0 22-23 36.90466425983723 39.0 36.0 40.0 31.0 41.0 24-25 36.69327265391618 38.5 35.0 40.0 30.0 41.0 26-27 36.358373278758094 38.0 35.0 40.0 30.0 41.0 28-29 36.172500893676535 38.0 35.0 40.0 29.5 41.0 30-31 35.75432591703755 38.0 34.0 40.0 28.5 41.0 32-33 35.41282169742099 38.0 34.0 40.0 27.0 41.0 34-35 35.18799755832938 38.0 34.0 40.0 27.0 41.0 36-37 35.0322212722191 38.0 33.5 40.0 26.5 41.0 38-39 34.72453230928107 38.0 33.0 40.0 25.5 41.0 40-41 34.40886213550018 37.0 33.0 40.0 24.5 41.0 42-43 34.043517552643294 37.0 33.0 40.0 24.0 41.0 44-45 33.557218293297325 37.0 32.0 39.5 23.0 40.5 46-47 32.939247137622004 36.5 31.0 39.0 20.5 40.0 48-49 32.32447147656268 36.0 30.5 39.0 17.5 40.0 50-51 31.696483827067844 35.5 30.0 39.0 14.0 40.0 52-53 30.920093402263145 35.0 28.5 38.0 9.0 40.0 54-55 30.31887883836684 34.0 28.0 38.0 7.5 40.0 56-57 29.757093492153416 34.0 27.0 38.0 4.5 40.0 58-59 29.009373255762906 33.5 26.0 37.5 2.0 39.5 60-61 28.353461298057326 33.0 25.5 37.0 2.0 39.0 62-63 27.634186315355976 32.5 23.5 36.5 2.0 39.0 64-65 26.8224351483503 31.5 22.0 36.0 2.0 39.0 66-67 26.054808397841796 31.0 20.5 35.0 2.0 38.0 68-69 25.28634023258585 31.0 18.5 35.0 2.0 38.0 70-71 24.409882076507074 30.0 15.0 34.0 2.0 37.0 72-73 23.66337535877716 29.0 10.0 34.0 2.0 36.0 74-75 22.876256843680817 29.0 7.5 34.0 2.0 36.0 76-77 22.122715480568022 27.5 7.0 32.5 2.0 35.0 78-79 22.618122714853428 29.0 4.5 34.0 2.0 35.0 80-81 22.009315558751652 29.0 2.0 34.0 2.0 35.0 82-83 21.28776405267152 27.5 2.0 33.0 2.0 35.0 84-85 20.414335407777806 26.5 2.0 33.0 2.0 35.0 86-87 19.468609272076947 25.5 2.0 32.0 2.0 34.0 88-89 18.329648738086924 24.5 2.0 32.0 2.0 34.0 90-91 17.346502745834215 21.0 2.0 31.0 2.0 34.0 92-93 16.178863870591456 15.5 2.0 31.0 2.0 34.0 94-95 14.996684303277231 4.5 2.0 30.5 2.0 34.0 96-97 13.631664579679363 2.0 2.0 30.0 2.0 34.0 98-99 12.130939186095857 2.0 2.0 29.0 2.0 33.5 100-101 9.61461030476983 2.0 2.0 22.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28753589030890936 1101 2 0.2956579279920284 1101 3 0.3516621109807616 1101 4 0.3389761047500528 1101 5 0.26639146852163975 1101 6 0.211965958067438 1101 7 0.2820343579382225 1101 8 0.3392076658718608 1101 9 0.4190806041498263 1101 10-11 0.3610854225691611 1101 12-13 0.4750903340048396 1101 14-15 0.5788052500446312 1101 16-17 0.6473272274405488 1101 18-19 0.6485183377342452 1101 20-21 0.5762926750121906 1101 22-23 0.4152362199880173 1101 24-25 0.5792902760285443 1101 26-27 0.45141406289726405 1101 28-29 0.4792677127587126 1101 30-31 0.6520352324877123 1101 32-33 0.5878203484954057 1101 34-35 0.7536102906493412 1101 36-37 0.7639476674633485 1101 38-39 0.8459709329044642 1101 40-41 0.8827728912866846 1101 42-43 0.9220732685586839 1101 44-45 0.8594155070220708 1101 46-47 0.878502157077385 1101 48-49 0.8868319998603127 1101 50-51 0.8321148137499996 1101 52-53 0.8225630435226208 1101 54-55 1.1576461686811435 1101 56-57 0.9811150615321971 1101 58-59 1.402114468446836 1101 60-61 1.1148855656247285 1101 62-63 1.361929691578279 1101 64-65 1.1563273121455957 1101 66-67 0.9176525956459862 1101 68-69 1.2026223370895224 1101 70-71 1.2251330239353813 1101 72-73 1.3167287071614524 1101 74-75 1.2544773504786555 1101 76-77 1.186742682753522 1101 78-79 1.4867068908368672 1101 80-81 1.7057109437378308 1101 82-83 1.6708593720194784 1101 84-85 1.865974686118495 1101 86-87 1.9256785709746325 1101 88-89 1.8035038367845786 1101 90-91 1.8254505125837035 1101 92-93 1.951490554878987 1101 94-95 1.4531078220349176 1101 96-97 1.3731754769435618 1101 98-99 1.5550458101824471 1101 100-101 1.308786864691383 1102 1 0.3627330910796438 1102 2 0.39495631936962994 1102 3 0.36959354765163255 1102 4 0.28249081269364495 1102 5 0.19431728174085805 1102 6 0.3048702766418856 1102 7 0.3805044300861553 1102 8 0.3795082250084505 1102 9 0.42320446869388206 1102 10-11 0.5527383120607396 1102 12-13 0.4935749685846176 1102 14-15 0.6269486666376949 1102 16-17 0.6442137828878387 1102 18-19 0.5843800289690293 1102 20-21 0.6373462475364207 1102 22-23 0.5614797373979243 1102 24-25 0.581906250571592 1102 26-27 0.6405414653185559 1102 28-29 0.7369823679015326 1102 30-31 0.7608037078343131 1102 32-33 0.6436066494519608 1102 34-35 0.8087517804080733 1102 36-37 1.137076068483566 1102 38-39 1.0105693345832876 1102 40-41 0.9592022978295986 1102 42-43 0.8400219583619943 1102 44-45 0.9459961163025099 1102 46-47 1.001883585276481 1102 48-49 1.1506184629343892 1102 50-51 1.4299207950986066 1102 52-53 1.2471305471772993 1102 54-55 1.3198592473999007 1102 56-57 1.529938471077653 1102 58-59 1.738985116111973 1102 60-61 1.5330941782308294 1102 62-63 1.6801882059850684 1102 64-65 1.6138515354872531 1102 66-67 1.6417654384499691 1102 68-69 1.6555394393063096 1102 70-71 1.7727681345383743 1102 72-73 1.7382840897165934 1102 74-75 1.8646404305765358 1102 76-77 1.739446076920327 1102 78-79 2.043211462494785 1102 80-81 2.043357138000786 1102 82-83 2.0740951927872935 1102 84-85 2.0812782990313785 1102 86-87 1.9903244820948238 1102 88-89 1.9211959530881408 1102 90-91 1.9686806423509005 1102 92-93 2.267580888114935 1102 94-95 2.086685344890393 1102 96-97 1.8519610196629372 1102 98-99 1.9228893487576943 1102 100-101 1.7078582915338814 1103 1 0.29700869993805057 1103 2 0.2746643306565879 1103 3 0.31672258463946434 1103 4 0.18507346752276987 1103 5 0.1872509633780055 1103 6 0.2780697344369827 1103 7 0.3131986079664699 1103 8 0.21146958842039254 1103 9 0.41140433044958513 1103 10-11 0.3516736514325345 1103 12-13 0.38277006958973203 1103 14-15 0.6634465076926617 1103 16-17 0.5712659775908762 1103 18-19 0.6072052995478288 1103 20-21 0.6007691105099937 1103 22-23 0.5879997514018882 1103 24-25 0.6558115921277619 1103 26-27 0.7411792742362096 1103 28-29 0.7667617455628104 1103 30-31 0.5536721573137982 1103 32-33 0.7559959717257456 1103 34-35 0.8512955652496785 1103 36-37 0.9616030605330153 1103 38-39 0.9647609495579559 1103 40-41 0.9077840968606878 1103 42-43 1.011375316172348 1103 44-45 1.0249801404084877 1103 46-47 1.002313728517926 1103 48-49 1.1210694924354812 1103 50-51 1.3549195233707643 1103 52-53 1.2032910960849605 1103 54-55 1.1453063719484362 1103 56-57 1.2657721709678356 1103 58-59 1.4677891533242295 1103 60-61 1.7806943529811967 1103 62-63 1.9645308423816203 1103 64-65 1.8113650831291679 1103 66-67 1.7155495918233399 1103 68-69 1.8963448469923314 1103 70-71 1.810771850975982 1103 72-73 1.5533284804991112 1103 74-75 1.65895939787368 1103 76-77 1.8037835366364803 1103 78-79 2.159542393010568 1103 80-81 2.288925540501154 1103 82-83 2.41055024394068 1103 84-85 2.2773413288556448 1103 86-87 2.1008271587078973 1103 88-89 2.198464790369812 1103 90-91 2.4363510763242893 1103 92-93 2.4814959845717546 1103 94-95 2.5679722436997583 1103 96-97 2.523099679890171 1103 98-99 2.649543417837952 1103 100-101 1.9026667992435442 1104 1 0.3537371746443938 1104 2 0.33155818366713063 1104 3 0.4353386469608047 1104 4 0.2873180068200938 1104 5 0.23128592323660513 1104 6 0.15617198786900843 1104 7 0.2897499287962617 1104 8 0.3698506383496394 1104 9 0.375501443881447 1104 10-11 0.49011792206311355 1104 12-13 0.4099008051210191 1104 14-15 0.6061812171345267 1104 16-17 0.6780679766243551 1104 18-19 0.5080789907398184 1104 20-21 0.4226144032209618 1104 22-23 0.4128608090717947 1104 24-25 0.5346864515860688 1104 26-27 0.5547632171974186 1104 28-29 0.695183467140339 1104 30-31 0.5822468339136009 1104 32-33 0.7870102437672202 1104 34-35 0.8828962268159799 1104 36-37 0.9146903721812691 1104 38-39 0.8553415667694324 1104 40-41 0.8625522015708995 1104 42-43 1.1739889092336782 1104 44-45 1.2001079336394156 1104 46-47 0.9736176063737005 1104 48-49 1.0806068234021922 1104 50-51 1.329220650717236 1104 52-53 1.2286408957529034 1104 54-55 1.2660799176844968 1104 56-57 1.231834379490234 1104 58-59 1.3043641978443077 1104 60-61 1.4020378300480978 1104 62-63 1.7767016370243702 1104 64-65 1.6872432903699526 1104 66-67 1.5390736866857146 1104 68-69 1.7977213758291448 1104 70-71 1.875353406039057 1104 72-73 1.798175439867677 1104 74-75 2.0037916775950215 1104 76-77 2.009581041653906 1104 78-79 2.275049847576991 1104 80-81 2.2025062847327206 1104 82-83 2.3902424916402154 1104 84-85 2.574561513344033 1104 86-87 2.668333095935022 1104 88-89 2.679735830146747 1104 90-91 2.570121750769136 1104 92-93 2.8537491418572714 1104 94-95 2.58343929605447 1104 96-97 2.858105024157764 1104 98-99 2.38195501709526 1104 100-101 2.0129674917751466 1105 1 0.24715263495040674 1105 2 0.3065688734821812 1105 3 0.2574786706587062 1105 4 0.2383611009419795 1105 5 0.11562495780463422 1105 6 0.22802596241952244 1105 7 0.38385597691330986 1105 8 0.2509455072384128 1105 9 0.43464175092384494 1105 10-11 0.30963726676456815 1105 12-13 0.36194024152306525 1105 14-15 0.5529706020193856 1105 16-17 0.40719353687369875 1105 18-19 0.45743290089384914 1105 20-21 0.48428690850146694 1105 22-23 0.5060303392351031 1105 24-25 0.4692041863756202 1105 26-27 0.32918416408320184 1105 28-29 0.5148587801072111 1105 30-31 0.674521768229198 1105 32-33 0.7976491212078471 1105 34-35 0.8582354674527366 1105 36-37 0.8555515462578214 1105 38-39 0.6385118696904613 1105 40-41 0.5747832818861909 1105 42-43 0.8522234154414932 1105 44-45 0.8526474730758196 1105 46-47 1.0125932516263916 1105 48-49 1.221730541912322 1105 50-51 1.2459378471522378 1105 52-53 1.285165997499334 1105 54-55 1.4280749849144208 1105 56-57 1.431852603693006 1105 58-59 1.5344426971488225 1105 60-61 1.5897204584546714 1105 62-63 1.6380953699593057 1105 64-65 1.755119880962912 1105 66-67 1.6907695034906176 1105 68-69 1.9414718910009547 1105 70-71 2.081569211510182 1105 72-73 2.0638752363748125 1105 74-75 2.1228863919322514 1105 76-77 1.8117564190172537 1105 78-79 2.1277954557373135 1105 80-81 2.160343464682235 1105 82-83 2.34316815234806 1105 84-85 2.2936193928968613 1105 86-87 2.395540800329435 1105 88-89 2.4242610993240206 1105 90-91 2.530388223387753 1105 92-93 2.8496746480135755 1105 94-95 2.6467246754785076 1105 96-97 2.5134536022835707 1105 98-99 2.19735350249014 1105 100-101 1.709163591924547 1106 1 -0.07081273301496083 1106 2 0.058971470884781496 1106 3 0.08735962303966005 1106 4 0.06207322215410471 1106 5 -0.0465178993382267 1106 6 -0.05956061766706 1106 7 0.05861788167521098 1106 8 0.07131347260638421 1106 9 -0.22324785946576498 1106 10-11 0.10605501568231546 1106 12-13 0.08366643199925505 1106 14-15 0.07183964963843437 1106 16-17 0.08879526847542962 1106 18-19 0.09362337708432733 1106 20-21 0.20885400806857035 1106 22-23 0.22764289334766374 1106 24-25 0.13368470585614034 1106 26-27 0.1704287528277959 1106 28-29 0.24210986235829068 1106 30-31 0.3684395171469461 1106 32-33 0.3463828874416137 1106 34-35 0.23449087870815077 1106 36-37 0.390508256214531 1106 38-39 0.24211035453893714 1106 40-41 0.37102787063077614 1106 42-43 0.4459301253982133 1106 44-45 0.34576976744811105 1106 46-47 -0.3901664886333478 1106 48-49 0.10713638606816289 1106 50-51 0.5294172843816831 1106 52-53 0.5070437031270387 1106 54-55 0.601132344221778 1106 56-57 0.46400628334668426 1106 58-59 0.291082307538435 1106 60-61 0.47082976581397773 1106 62-63 0.3524027292666645 1106 64-65 0.5466675000105319 1106 66-67 0.6514892004603148 1106 68-69 0.39356604684511254 1106 70-71 0.5843073067482791 1106 72-73 1.1165592190588 1106 74-75 1.1066905044863695 1106 76-77 1.2071243843852208 1106 78-79 1.5123679665598253 1106 80-81 0.9809495252882954 1106 82-83 1.6546291913090982 1106 84-85 1.7043012110786826 1106 86-87 1.9536143934030257 1106 88-89 2.037659367722288 1106 90-91 1.7370657125652436 1106 92-93 1.7845339553728827 1106 94-95 1.8183372295910605 1106 96-97 1.728323732153699 1106 98-99 1.4572019873386264 1106 100-101 1.0748021200630742 1107 1 0.07826207723867284 1107 2 0.0876461503177417 1107 3 0.16190527494389073 1107 4 0.07587226580986339 1107 5 0.08025917546638794 1107 6 0.08057567266461518 1107 7 0.084416479864025 1107 8 0.1677025118638582 1107 9 0.023270731730910654 1107 10-11 0.2039905019179571 1107 12-13 0.19046847268899114 1107 14-15 0.2569981287353187 1107 16-17 0.2497652445668237 1107 18-19 0.17906795592070068 1107 20-21 0.36241041320666767 1107 22-23 0.4742231591617383 1107 24-25 0.38379022231586646 1107 26-27 0.38849539806693656 1107 28-29 0.40931715109982036 1107 30-31 0.14882522667993925 1107 32-33 0.2726497193257629 1107 34-35 0.5649774526725793 1107 36-37 0.6310011315627335 1107 38-39 0.6997721041526788 1107 40-41 0.740383127704682 1107 42-43 0.6105577234855204 1107 44-45 0.7879999639046744 1107 46-47 0.7774627954617941 1107 48-49 0.8658265863591268 1107 50-51 0.9840125720179991 1107 52-53 1.0922814076753156 1107 54-55 1.0421566080735438 1107 56-57 1.211876993612723 1107 58-59 1.0377112786789446 1107 60-61 1.149822404331644 1107 62-63 1.3157357947559447 1107 64-65 1.1477777840943943 1107 66-67 1.1673614275695208 1107 68-69 1.2388835689211 1107 70-71 1.3711250092665779 1107 72-73 1.4062126147575036 1107 74-75 1.2601769177443636 1107 76-77 1.4668949830025824 1107 78-79 1.8002791071807742 1107 80-81 1.724516334053277 1107 82-83 1.8617996585419014 1107 84-85 1.9450885034290586 1107 86-87 2.1384964997286566 1107 88-89 2.220788293752218 1107 90-91 2.0489475566856434 1107 92-93 2.0687298875265725 1107 94-95 1.8951580052674348 1107 96-97 1.9442338803419457 1107 98-99 1.8656080614778592 1107 100-101 1.6499160242707642 1108 1 0.1751748170773446 1108 2 0.06629451559638255 1108 3 0.18460454626898226 1108 4 0.16742127924807448 1108 5 0.10704822250427526 1108 6 0.16933194840975574 1108 7 0.17032541015466052 1108 8 0.13017868490769757 1108 9 0.14452871759758779 1108 10-11 0.21784359238545647 1108 12-13 0.281137333435602 1108 14-15 0.3128933891134196 1108 16-17 0.3617029297839025 1108 18-19 0.2948275623245493 1108 20-21 0.4733019796116409 1108 22-23 0.22317876844333995 1108 24-25 0.25721655105402874 1108 26-27 0.25196382869793865 1108 28-29 0.41592171791234733 1108 30-31 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-1.437645612973899 2316 62-63 -1.638861475278791 2316 64-65 -1.7611449999894653 2316 66-67 -1.5887096631760471 2316 68-69 -1.6850087637609477 2316 70-71 -1.6370468599183887 2316 72-73 -2.1225979779108997 2316 74-75 -2.012225215210602 2316 76-77 -2.002960842887507 2316 78-79 -2.28509888950078 2316 80-81 -2.2413752853177655 2316 82-83 -2.426668157175751 2316 84-85 -2.3466931071031354 2316 86-87 -2.5858505687181825 2316 88-89 -2.864258245914076 2316 90-91 -2.5883840980408195 2316 92-93 -2.459618506748331 2316 94-95 -2.2202991340453035 2316 96-97 -2.3962264572402407 2316 98-99 -2.110979830843192 2316 100-101 -1.786111705694501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 44.0 4 129.0 5 198.0 6 456.0 7 1277.0 8 2715.0 9 4837.0 10 7439.0 11 11293.0 12 15834.0 13 21642.0 14 28331.0 15 36685.0 16 45386.0 17 53219.0 18 52199.0 19 50034.0 20 51490.0 21 54675.0 22 57732.0 23 62944.0 24 69437.0 25 77128.0 26 88297.0 27 99661.0 28 114208.0 29 128280.0 30 141462.0 31 156065.0 32 170875.0 33 184175.0 34 192415.0 35 183456.0 36 143951.0 37 71551.0 38 12114.0 39 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.16288987373527 20.95442762772807 16.467597625219497 21.415084873317166 2 17.965804237015558 22.344524884341794 36.44263348949613 23.24703738914651 3 20.925493508040997 24.53239289992286 30.54730632304891 23.994807268987227 4 15.673685772878642 18.373947708947842 34.51100737727365 31.441359140899866 5 14.17360214752435 37.417842889086124 33.627510931277044 14.781044032112483 6 33.15027771912844 36.57580781041933 15.634970242139303 14.638944228312928 7 28.636280967721028 33.36162437991391 19.681746630682685 18.32034802168237 8 27.484891117795975 34.79075426299385 18.637639774145466 19.086714845064712 9 26.73796567864019 16.342511199700642 19.201230869573397 37.71829225208577 10-11 25.680228948430162 26.47759746300388 26.418060836899333 21.42411275166663 12-13 26.97203576378509 23.604913444030764 26.980334935331268 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22.517831242586432 25.534247400102604 29.26959952402634 22.678321833284624 40-41 22.953886522803373 25.634855481336217 29.18751668471804 22.22374131114237 42-43 22.77879294475097 25.656545680736876 28.954529462268574 22.610131912243585 44-45 22.699921853015933 25.696998762777284 29.33789978203305 22.26517960217373 46-47 23.419948532593583 26.28140672002943 28.098653527356955 22.199991220020028 48-49 22.998530398590187 26.535587140256002 27.736144878031443 22.72973758312237 50-51 23.168112999603647 26.525255498378623 27.97780412710783 22.328827374909903 52-53 23.895551685860678 26.079069991073688 27.817819596497205 22.207558726568426 54-55 23.008150629332942 26.51796560592881 28.12733286129966 22.34655090343859 56-57 23.18405973731136 26.64805450277092 27.629656263784046 22.538229496133674 58-59 23.019948083338473 26.219795866948658 27.65380807562311 23.106447974089757 60-61 22.52169386718399 26.578316376125983 28.069156975589564 22.830832781100465 62-63 22.860392897029165 26.594118879303096 28.14714634730699 22.398341876360742 64-65 22.4401514630409 26.615692536131995 27.93826570176684 23.005890299060265 66-67 22.25986895813965 26.73703844392129 27.890767927210952 23.11232467072811 68-69 23.134676769022043 26.09659682147546 28.03570879742483 22.733017612077667 70-71 22.85074839969086 26.533596928008507 27.430597873111235 23.1850567991894 72-73 22.740529169503613 26.598787651749966 27.417332812945205 23.24335036580122 74-75 22.79760375173309 27.154629772700993 27.09606382914217 22.951702646423744 76-77 22.954993919227682 26.529525992556675 27.640587142225236 22.874892945990403 78-79 22.96931618368009 26.81121796594228 27.54438090681604 22.675084943561593 80-81 23.34425938828032 26.86471291295762 27.062958499113005 22.728069199649052 82-83 23.296609907211383 27.01292248599555 27.056104785142445 22.634362821650623 84-85 23.082822326394762 27.20463018388305 27.022208599552112 22.690338890170082 86-87 23.51335920034839 27.0311422331113 26.57408257221185 22.881415994328464 88-89 23.061318416026342 27.348607673849873 26.264683178072563 23.325390732051222 90-91 22.98555198654435 27.231843590604633 26.586180608322884 23.19642381452814 92-93 23.27446601330709 26.73758819277446 26.728011737639424 23.25993405627903 94-95 22.960676686431164 26.43126026010349 26.955130611078225 23.65293244238712 96-97 23.286944472849772 25.91201486752209 26.952298081521686 23.848742578106457 98-99 22.94761389363827 26.55178219426623 26.329249737259026 24.171354174836473 100-101 23.32590361407999 26.021593366398754 26.786984770992024 23.86551824852923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 849.0 1 612.5 2 500.5 3 663.5 4 629.0 5 630.0 6 1099.5 7 2515.0 8 3941.0 9 4292.0 10 3504.0 11 2561.5 12 2373.0 13 2493.5 14 2726.5 15 3018.5 16 3316.5 17 3668.0 18 4063.5 19 4579.5 20 5199.0 21 6077.5 22 7212.5 23 8613.0 24 10479.0 25 12973.0 26 16103.5 27 19445.0 28 22557.0 29 25744.0 30 29368.0 31 35359.5 32 43608.0 33 44435.5 34 40417.5 35 40547.5 36 43537.5 37 47237.0 38 50785.5 39 53564.0 40 56740.0 41 62395.0 42 66434.0 43 68987.5 44 71877.0 45 77017.0 46 81240.5 47 83370.5 48 84135.0 49 82272.5 50 81794.5 51 81895.0 52 82168.5 53 84935.0 54 88012.0 55 85574.0 56 80564.0 57 75043.5 58 69137.5 59 61676.0 60 51209.5 61 43323.5 62 37559.5 63 29753.0 64 22055.5 65 16738.0 66 12879.0 67 9900.0 68 7550.5 69 5950.0 70 4670.5 71 3485.5 72 2646.5 73 2113.0 74 1653.0 75 1277.5 76 956.0 77 684.0 78 484.5 79 314.5 80 194.0 81 110.0 82 60.5 83 35.0 84 25.5 85 17.5 86 6.5 87 4.0 88 2.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0904714222104225E-4 2 0.03863191188244861 3 0.0 4 0.0 5 0.0400952418779959 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0050171314133050145 26-27 0.0013588064244367747 28-29 1.0452357111052113E-4 30-31 0.001107949853771524 32-33 2.7176128488735495E-4 34-35 0.009428026114169007 36-37 0.019859478510999017 38-39 0.0031775165617598427 40-41 8.570932831062733E-4 42-43 0.0010661404253273156 44-45 0.006605889694184936 46-47 0.0 48-49 0.0 50-51 3.762848559978761E-4 52-53 0.0 54-55 0.001107949853771524 56-57 0.0 58-59 0.019754954939888495 60-61 0.0 62-63 0.007776553690622773 64-65 0.013818016100810893 66-67 0.005288892698192369 68-69 0.014612395241250855 70-71 6.271414266631268E-5 72-73 2.0904714222104227E-5 74-75 0.020214858652774788 76-77 0.044840612006413566 78-79 0.027531508630511268 80-81 0.024207659069196693 82-83 0.03256954475803839 84-85 0.015323155524802397 86-87 0.008926312972838505 88-89 0.0727484054929227 90-91 0.02266071021676098 92-93 0.021887235790543125 94-95 0.02360142235675567 96-97 0.034346445466917244 98-99 0.04776727199750816 100-101 0.011852972963933096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2391805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.996794183574195 #Duplication Level Percentage of deduplicated Percentage of total 1 73.2472486835313 35.156331195765276 2 14.004819979231387 13.443729242423533 3 5.1438770560538245 7.406688250950749 4 2.4665452638619247 4.73545061496202 5 1.3951441491419514 3.348122329139199 6 0.8333068367992129 2.3997634042570244 7 0.5633329610523183 1.8926723331916078 8 0.3963579434324344 1.5219128507161037 9 0.29928312047299843 1.292816730236431 >10 1.4898546320288273 12.801366001372505 >50 0.09621702218575078 3.167896061548911 >100 0.053189937862147235 5.011723317338981 >500 0.005411207173009003 1.7048981574167688 >1k 0.005062097032814588 4.705257518256347 >5k 2.618326051455821E-4 0.9250242293932379 >10k+ 8.727753504852737E-5 0.4863477630312241 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11610 0.4854074642372601 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9737 0.4070984047612577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7245 0.3029093090782903 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5100 0.21322808506546312 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4412 0.1844631982958477 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4212 0.176101312607006 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4080 0.17058246805237048 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4077 0.17045703976703788 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3587 0.14997041982937573 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3469 0.14503690727295912 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3300 0.1379711138658879 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3135 0.1310725581725935 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2755 0.1151849753637943 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2633 0.11008422509360086 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 2581 0.10791013481450203 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 2501 0.10456538053896536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.361885688841691E-5 2 0.0 0.0 0.0 0.0 8.361885688841691E-5 3 0.0 0.0 0.0 0.0 8.361885688841691E-5 4 0.0 0.0 0.0 0.0 8.361885688841691E-5 5 0.0 0.0 0.0 0.0 8.361885688841691E-5 6 0.0 0.0 0.0 0.0 1.6723771377683382E-4 7 0.0 0.0 0.0 0.0 1.6723771377683382E-4 8 0.0 0.0 0.0 0.0 1.6723771377683382E-4 9 0.0 0.0 0.0 0.0 1.6723771377683382E-4 10-11 0.0 0.0 0.0 0.0 1.6723771377683382E-4 12-13 0.0 0.0 0.0 0.0 2.2995185644314649E-4 14-15 0.0 0.0 0.0 0.0 3.3447542755366764E-4 16-17 0.0 0.0 0.0 0.0 4.180942844420845E-4 18-19 0.0 0.0 0.0 0.0 5.017131413305014E-4 20-21 0.0 0.0 0.0 4.1809428444208455E-5 5.017131413305014E-4 22-23 0.0 0.0 0.0 1.463329995547296E-4 5.853319982189184E-4 24-25 0.0 0.0 0.0 3.3447542755366764E-4 5.853319982189184E-4 26-27 0.0 0.0 0.0 6.062367124410226E-4 5.853319982189184E-4 28-29 0.0 0.0 0.0 0.0016932818519904424 5.853319982189184E-4 30-31 0.0 0.0 0.0 0.005288892698192369 6.062367124410226E-4 32-33 0.0 0.0 0.0 0.01394344438614352 6.271414266631268E-4 34-35 2.0904714222104227E-5 0.0 0.0 0.028534934913172272 6.271414266631268E-4 36-37 4.1809428444208455E-5 0.0 0.0 0.05006679056193962 6.271414266631268E-4 38-39 4.1809428444208455E-5 0.0 0.0 0.08602289902395889 6.271414266631268E-4 40-41 4.1809428444208455E-5 0.0 0.0 0.12335871862463704 6.271414266631268E-4 42-43 4.1809428444208455E-5 0.0 0.0 0.16403929250085186 6.271414266631268E-4 44-45 4.1809428444208455E-5 0.0 0.0 0.21977126061698174 6.48046140885231E-4 46-47 4.1809428444208455E-5 0.0 0.0 0.2875443441250436 7.316649977736479E-4 48-49 4.1809428444208455E-5 0.0 0.0 0.35970741761974745 9.198074257725859E-4 50-51 4.1809428444208455E-5 0.0 0.0 0.4417166115130623 0.0010034262826610029 52-53 4.1809428444208455E-5 0.0 0.0 0.5178306759957438 0.0010661404253273156 54-55 4.1809428444208455E-5 0.0 0.0 0.6064248548690215 0.0012124734248820453 56-57 4.1809428444208455E-5 0.0 0.0 0.7120772805475363 0.0012751875675483578 58-59 8.361885688841691E-5 0.0 0.0 0.8219524584989162 0.0013379017102146703 60-61 8.361885688841691E-5 0.0 0.0 0.9459174138359941 0.0014006158528809833 62-63 8.361885688841691E-5 0.0 0.0 1.071408413311286 0.0014633299955472958 64-65 8.361885688841691E-5 0.0 0.0 1.205240393761197 0.0015469488524357128 66-67 8.361885688841691E-5 0.0 0.0 1.3702622078304878 0.0015678535666578172 68-69 8.361885688841691E-5 0.0 0.0 1.551568794278798 0.0015887582808799213 70-71 1.2542828533262536E-4 0.0 0.0 1.7342550918657667 0.0016096629951020255 72-73 1.2542828533262536E-4 0.0 0.0 1.9377415801037292 0.0017350912804346508 74-75 1.2542828533262536E-4 0.0 0.0 2.147959386321209 0.001860519565767276 76-77 1.2542828533262536E-4 0.0 0.0 2.3805034273278967 0.0018814242799893805 78-79 1.2542828533262536E-4 0.0 0.0 2.6426694483873057 0.0018814242799893805 80-81 1.2542828533262536E-4 0.0 0.0 2.9171901555519786 0.0019023289942114846 82-83 1.2542828533262536E-4 0.0 0.0 3.2052362128183525 0.0020068525653220057 84-85 1.2542828533262536E-4 0.0 0.0 3.508333664324642 0.0020068525653220057 86-87 1.2542828533262536E-4 0.0 0.0 3.815821105817573 0.002090471422210423 88-89 1.2542828533262536E-4 0.0 0.0 4.124792782020274 0.0021740902790988396 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4865 0.0 64.17926 1 GTATCAA 8965 0.0 43.680653 1 GTGGTAT 1800 0.0 33.79478 1 TGGTATC 1725 0.0 32.783928 2 AGGTAAG 1745 0.0 31.591167 2 TATCAAC 12580 0.0 30.901232 2 ATCAACG 12900 0.0 30.08589 3 TCAACGC 13065 0.0 29.74229 4 CAACGCA 13410 0.0 28.977106 5 AAGGTAA 1830 0.0 28.566286 1 AACGCAG 13925 0.0 27.99664 6 TAAGGTG 2235 0.0 27.843529 5 GTAAGGT 2210 0.0 27.513649 4 GTGTAAG 830 0.0 26.338554 1 GGTAAGG 2305 0.0 24.524858 3 ACGCAGA 15925 0.0 24.48058 7 CGCAGAG 16335 0.0 23.807991 8 TAAGGTA 930 0.0 22.985825 4 GTCGTAA 170 3.1376094E-6 22.364197 1 AGAGTAC 15855 0.0 21.758419 10-11 >>END_MODULE