FastQCFastQC Report
Thu 26 May 2016
SRR938338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530519
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114050.4506980583824899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81270.32115941433358136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.2546513185635042No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA64140.2534657910096703No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA53660.21205136179574233No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53490.2113795628485698No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA52800.20865284947475202No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA43530.17202004806128704No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42730.1688586412510635No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39160.15475086336044108No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA37030.14633361772822098No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA34680.1370469852231894No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34090.13471544770064955No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA30660.12116091600181624No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT30130.11906648399004315No Hit
TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA26870.10618375123838232No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG26670.10539339953582645No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA26610.10515629402505969No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT26020.10282475650251985No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG25380.10029563105434101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.057.052381
GTATCAA80250.046.384961
TATCAAC106150.034.7417952
ATCAACG107400.034.2931943
TCAACGC108700.033.7956284
CAACGCA110700.033.2689325
AACGCAG114700.032.145076
ACGCAGA132200.027.8511667
CGCAGAG134350.027.3347428
TGGTATC18750.026.613292
GTGGTAT20000.026.4185181
TAAGGTG18250.025.778475
GCAGAGT146650.024.5546939
AAGGTAA16250.024.3130911
AGGTAAG17150.024.108252
GTAAGGT18850.023.9509544
GTACATG82750.023.4697041
GGTAAGG19050.023.4500313
TACATGG83200.023.076432
AGAGTAC132550.022.4161310-11
Produced by FastQC (version 0.11.5)