FastQCFastQC Report
Thu 26 May 2016
SRR938338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530519
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120440.47594979527915027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97030.3834391284949846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75390.29792307427843856No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52720.20833670879372967No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA48440.19142318235903386No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG43760.1729289525192263No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42810.16917478193208588No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42390.16751504335671852No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39130.1546323106050577No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35600.14068260305494643No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT34430.13605904559499454No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33340.131751628816065No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29100.11499617272188037No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA28140.11120248454961215No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA27460.10851528876092216No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25760.1017972992891972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.062.7497561
GTATCAA99150.045.7081951
TGGTATC18300.034.561652
TATCAAC131000.034.2684632
ATCAACG134600.033.2314643
TCAACGC137000.032.6840064
CAACGCA139350.032.132825
AGGTAAG19450.031.7846662
GTGGTAT20950.031.551841
TAAGGTG21000.031.463025
AACGCAG144400.031.0832316
AAGGTAA19000.029.2837121
GTAAGGT22650.027.701974
ACGCAGA164150.027.198727
CGCAGAG166300.026.7899658
GGTAAGG24150.025.5876943
GCAGAGT180650.024.4252649
GTGTAAG10150.024.3630241
GGTGTGC30350.023.6308758
AAGGTGT30700.022.8973336
Produced by FastQC (version 0.11.5)