##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938338_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2530519 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.223854869297565 31.0 31.0 33.0 30.0 34.0 2 31.484534990648164 31.0 31.0 34.0 30.0 34.0 3 31.518702685101356 31.0 31.0 34.0 30.0 34.0 4 35.30561872880622 37.0 35.0 37.0 33.0 37.0 5 35.15000005927638 37.0 35.0 37.0 32.0 37.0 6 35.22015878956056 37.0 35.0 37.0 32.0 37.0 7 35.1452453034338 37.0 35.0 37.0 32.0 37.0 8 35.127950432302626 37.0 35.0 37.0 32.0 37.0 9 36.600768458960395 38.0 35.0 39.0 32.0 39.0 10-11 36.45762055135725 38.0 35.0 39.0 32.0 39.0 12-13 36.32379997937182 38.0 35.0 39.0 32.0 39.0 14-15 37.131266352870696 39.0 36.0 40.0 31.5 41.0 16-17 36.99473230590246 38.0 36.0 40.0 31.0 41.0 18-19 37.13359512416228 38.5 36.0 40.0 31.5 41.0 20-21 37.1843382721094 39.0 36.0 40.0 31.0 41.0 22-23 37.03933659458791 39.0 36.0 40.0 31.0 41.0 24-25 36.868065997528575 39.0 36.0 40.0 31.0 41.0 26-27 36.55354020262247 38.0 35.0 40.0 30.0 41.0 28-29 36.36953091440925 38.0 35.0 40.0 30.0 41.0 30-31 35.9926479508749 38.0 34.5 40.0 29.5 41.0 32-33 35.64863117012755 38.0 34.0 40.0 28.5 41.0 34-35 35.38300660852576 38.0 34.0 40.0 27.0 41.0 36-37 35.27592995745141 38.0 34.0 40.0 27.0 41.0 38-39 35.04398544330235 38.0 33.5 40.0 26.5 41.0 40-41 34.74489798337811 38.0 33.0 40.0 25.5 41.0 42-43 34.39279353365851 37.5 33.0 40.0 24.5 41.0 44-45 33.984797189825485 37.0 33.0 40.0 24.0 41.0 46-47 33.41411583947799 37.0 32.0 39.5 22.5 40.5 48-49 32.84516516177116 36.5 31.0 39.0 19.5 40.0 50-51 32.26484468205929 36.0 30.5 39.0 17.0 40.0 52-53 31.535624312641005 35.0 30.0 39.0 13.5 40.0 54-55 30.999198978549458 35.0 29.0 38.0 9.0 40.0 56-57 30.466338525812294 34.0 28.0 38.0 8.0 40.0 58-59 29.80334903630441 34.0 27.5 38.0 6.5 40.0 60-61 29.23007750583971 34.0 26.0 37.5 2.0 39.5 62-63 28.577359822234094 33.0 26.0 37.0 2.0 39.0 64-65 27.851436602530946 32.5 25.0 36.0 2.0 39.0 66-67 27.15347385259704 32.0 23.0 36.0 2.0 38.5 68-69 26.412721659074677 31.0 22.0 35.0 2.0 38.0 70-71 25.627060496285544 31.0 20.0 35.0 2.0 37.5 72-73 24.988729387133628 30.0 18.5 34.0 2.0 37.0 74-75 24.288901012005837 30.0 17.0 34.0 2.0 36.0 76-77 23.437782723623098 28.5 16.0 32.5 2.0 35.0 78-79 23.99284494603676 30.0 14.5 34.0 2.0 35.0 80-81 23.54854992197253 30.0 8.5 34.0 2.0 35.0 82-83 22.851861021395216 29.0 6.0 34.0 2.0 35.0 84-85 22.07220811224891 29.0 2.0 34.0 2.0 35.0 86-87 21.21096581373228 28.0 2.0 33.0 2.0 35.0 88-89 20.237072513583183 26.0 2.0 32.5 2.0 34.0 90-91 19.37164352451019 25.5 2.0 32.0 2.0 34.0 92-93 18.298119279088596 24.5 2.0 32.0 2.0 34.0 94-95 17.161054510952102 20.5 2.0 31.5 2.0 34.0 96-97 15.807150430405777 9.0 2.0 31.0 2.0 34.0 98-99 14.301140991235394 2.0 2.0 31.0 2.0 34.0 100-101 11.58403651582936 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3225631813511498 1101 2 0.43337016802542294 1101 3 0.4210507654524598 1101 4 0.3460348660875425 1101 5 0.23828830399352796 1101 6 0.29158136317166594 1101 7 0.3549179290425215 1101 8 0.34543560110759586 1101 9 0.3903777977069183 1101 10-11 0.4274330875555492 1101 12-13 0.46777388000420217 1101 14-15 0.658524446030178 1101 16-17 0.6775568952283777 1101 18-19 0.6590255083438237 1101 20-21 0.6228966674514425 1101 22-23 0.6308769796709228 1101 24-25 0.5858389555628776 1101 26-27 0.6531288845450547 1101 28-29 0.7566419403147933 1101 30-31 0.7892447990217093 1101 32-33 0.734564393927883 1101 34-35 0.642175506761447 1101 36-37 0.8823187961936583 1101 38-39 0.9415838128277727 1101 40-41 1.1477783182371084 1101 42-43 1.0523641493249514 1101 44-45 0.9674351774003966 1101 46-47 1.1246050720168128 1101 48-49 1.0841452764980346 1101 50-51 1.2182876973478542 1101 52-53 1.2348388345404828 1101 54-55 1.045196663353373 1101 56-57 1.0628066066119892 1101 58-59 1.0831387654325688 1101 60-61 1.2339683600180678 1101 62-63 1.2068634409618717 1101 64-65 1.0575772653965778 1101 66-67 1.0770151246507496 1101 68-69 1.013576714686934 1101 70-71 0.969341090459622 1101 72-73 0.8992304310161039 1101 74-75 0.9585303701901537 1101 76-77 0.9457248389570658 1101 78-79 1.0241845703498385 1101 80-81 1.1225576136243838 1101 82-83 1.0050528149739932 1101 84-85 1.1244497465976515 1101 86-87 1.1550917873156266 1101 88-89 1.2324633289359639 1101 90-91 1.168801198155304 1101 92-93 0.9936289689010103 1101 94-95 0.9130563370214304 1101 96-97 0.9421072463907176 1101 98-99 0.6957637800856702 1101 100-101 0.2871117912275718 1102 1 0.34541451392078315 1102 2 0.3000734571103365 1102 3 0.27221285596749567 1102 4 0.2495241418153995 1102 5 0.27974325280687395 1102 6 0.303449271567203 1102 7 0.3418251063982467 1102 8 0.22979886292377216 1102 9 0.25196763762689756 1102 10-11 0.42772373398305064 1102 12-13 0.3792008668089153 1102 14-15 0.5204449441302827 1102 16-17 0.5364757195695162 1102 18-19 0.5191040132512015 1102 20-21 0.5122806119103771 1102 22-23 0.39453402745063926 1102 24-25 0.5035558427013385 1102 26-27 0.4347330521058197 1102 28-29 0.326661913178107 1102 30-31 0.4064213954477296 1102 32-33 0.35497084994962336 1102 34-35 0.3372472605108996 1102 36-37 0.5333917516162074 1102 38-39 0.5805742753728822 1102 40-41 0.6354243264705488 1102 42-43 0.5745703419029766 1102 44-45 0.47083716676360865 1102 46-47 0.33207518700107386 1102 48-49 0.8473567808673081 1102 50-51 0.646145450945447 1102 52-53 0.5427907412061401 1102 54-55 0.5596652036176764 1102 56-57 0.6156928999456746 1102 58-59 0.5879630867678785 1102 60-61 0.8087558718614787 1102 62-63 0.8517889801466474 1102 64-65 0.7777237770782257 1102 66-67 0.7544053811947542 1102 68-69 0.5472876726910982 1102 70-71 0.6739138527561401 1102 72-73 0.6204550811608698 1102 74-75 0.711969829305211 1102 76-77 0.6573637692039078 1102 78-79 0.5226492018752715 1102 80-81 0.44043996190489665 1102 82-83 0.23335498954012124 1102 84-85 0.0519584867886671 1102 86-87 -0.041484962950953275 1102 88-89 0.20124841762537926 1102 90-91 0.35344388197992416 1102 92-93 0.47536881988034096 1102 94-95 0.4541521044250558 1102 96-97 0.3623881869970358 1102 98-99 0.5105470662654383 1102 100-101 0.04483158350979366 1103 1 0.24091264896845743 1103 2 0.3571473753609773 1103 3 0.3663186443268778 1103 4 0.2994815450645092 1103 5 0.3046506098060888 1103 6 0.2455441661559945 1103 7 0.25589091885569104 1103 8 0.2969732962686109 1103 9 0.3530429239632369 1103 10-11 0.44043198741697864 1103 12-13 0.4816891076744767 1103 14-15 0.6714635684506121 1103 16-17 0.5836961876997293 1103 18-19 0.46990428865036904 1103 20-21 0.4795828164147409 1103 22-23 0.5660241678270737 1103 24-25 0.5544039400019187 1103 26-27 0.6222097982766641 1103 28-29 0.6698775484577695 1103 30-31 0.7516617288237839 1103 32-33 0.765648079100032 1103 34-35 0.6666331124834599 1103 36-37 0.6760592997910138 1103 38-39 0.8024621063473418 1103 40-41 1.0461142207239433 1103 42-43 1.0583613649034334 1103 44-45 0.9496982825171827 1103 46-47 0.9815602519170525 1103 48-49 1.121735226618597 1103 50-51 1.0102062546410693 1103 52-53 1.4309466805730082 1103 54-55 1.2312217880519718 1103 56-57 1.2305937054589542 1103 58-59 1.3491221562608509 1103 60-61 1.4576128367707426 1103 62-63 1.2825312964705162 1103 64-65 1.1330354130747615 1103 66-67 1.2203137879337085 1103 68-69 1.1061407515139905 1103 70-71 0.6776353564335409 1103 72-73 0.486830798378012 1103 74-75 0.5861977194403423 1103 76-77 0.667827499085643 1103 78-79 0.8513458558568914 1103 80-81 0.9312561075054653 1103 82-83 0.8743362969937039 1103 84-85 0.8569418038567846 1103 86-87 1.1687546646161024 1103 88-89 0.9768257840298631 1103 90-91 0.9517896158704566 1103 92-93 0.9865085735001742 1103 94-95 0.9570365020746685 1103 96-97 0.9411770289267469 1103 98-99 0.9433684960779214 1103 100-101 0.5825154836690682 1104 1 0.24480480228396218 1104 2 0.25176480643931853 1104 3 0.19976165863702988 1104 4 0.22840067548938237 1104 5 0.1958947841656382 1104 6 0.1758705049429068 1104 7 0.18583068886748322 1104 8 0.18019019541360848 1104 9 0.24700214665490705 1104 10-11 0.36211376620676816 1104 12-13 0.3186802842268719 1104 14-15 0.5129862377380263 1104 16-17 0.47624281089589005 1104 18-19 0.42728823623188816 1104 20-21 0.36529152195581815 1104 22-23 0.45322156136106884 1104 24-25 0.45248450044454813 1104 26-27 0.5728496829358107 1104 28-29 0.5028340129720377 1104 30-31 0.555923456591394 1104 32-33 0.5594097780128209 1104 34-35 0.5369843779111392 1104 36-37 0.5063213003009679 1104 38-39 0.494324888767693 1104 40-41 0.6114100365059656 1104 42-43 0.6272283777707202 1104 44-45 0.5673182343179946 1104 46-47 0.5574690653052912 1104 48-49 0.7333985885612861 1104 50-51 0.7602170675394504 1104 52-53 0.8335208732905954 1104 54-55 0.7502968435347022 1104 56-57 0.631706165850968 1104 58-59 0.8043789341102539 1104 60-61 0.604596515971835 1104 62-63 0.6283197001179133 1104 64-65 0.6986792632801588 1104 66-67 0.7252321557582455 1104 68-69 0.5250239806657113 1104 70-71 0.39297223672427606 1104 72-73 0.3636744588155132 1104 74-75 0.3765853652168971 1104 76-77 0.2948532634350336 1104 78-79 0.3586839236673036 1104 80-81 0.38725631040813013 1104 82-83 0.21629466788628804 1104 84-85 0.17212566172494093 1104 86-87 0.14180451091366209 1104 88-89 0.05347520437886999 1104 90-91 -0.012402108029011316 1104 92-93 0.032688593984030234 1104 94-95 0.3696255828974415 1104 96-97 -0.10452171036894065 1104 98-99 -0.48354059924209913 1104 100-101 -0.3280215969757947 1105 1 0.2924496066505462 1105 2 0.2720465179869329 1105 3 0.19369226060029732 1105 4 0.21160873381087697 1105 5 0.1550390580033465 1105 6 0.3272843274713537 1105 7 0.25449606212682596 1105 8 0.19703990685069073 1105 9 0.2569498608544549 1105 10-11 0.22143564929601922 1105 12-13 0.3552813570053317 1105 14-15 0.46285542794656465 1105 16-17 0.23259778212059956 1105 18-19 0.4697516292630226 1105 20-21 0.41020709234701513 1105 22-23 0.3072750211383095 1105 24-25 0.47730596211235365 1105 26-27 0.5274269297982599 1105 28-29 0.39156123918031227 1105 30-31 0.42188004756101094 1105 32-33 0.5063277673488784 1105 34-35 0.590438318618645 1105 36-37 0.46326859617599325 1105 38-39 0.42261361987697654 1105 40-41 0.4397652445271305 1105 42-43 0.5354352092292984 1105 44-45 0.31932272582738364 1105 46-47 0.07653129893906652 1105 48-49 0.012176206674830325 1105 50-51 0.028992239073943438 1105 52-53 -0.037566803812431715 1105 54-55 -0.13269015314776667 1105 56-57 -0.2195900088619318 1105 58-59 0.009306313354780826 1105 60-61 -0.07105410253382871 1105 62-63 0.018515826661555934 1105 64-65 -0.08943067221305867 1105 66-67 -0.09739800963240342 1105 68-69 -0.35920435114853433 1105 70-71 -0.1515806944298923 1105 72-73 -0.07461028677600012 1105 74-75 0.06491894709915869 1105 76-77 0.04101407586127337 1105 78-79 -0.01932135446370964 1105 80-81 0.025595168992044393 1105 82-83 0.11686231234284605 1105 84-85 0.42743297540019753 1105 86-87 0.23107004407016518 1105 88-89 0.11999541378888168 1105 90-91 0.11823080077004633 1105 92-93 0.1476018750252095 1105 94-95 0.21829692609554385 1105 96-97 -0.036293243242436546 1105 98-99 -0.13860600652008692 1105 100-101 -0.5330700544875082 1106 1 0.08566757014361315 1106 2 0.1570299746210111 1106 3 0.1713408720728431 1106 4 0.21246019049451803 1106 5 0.09042153814510812 1106 6 -0.03710034606049817 1106 7 0.07833969943676777 1106 8 0.03604804841729248 1106 9 0.025759234189273172 1106 10-11 -0.07482660332799185 1106 12-13 0.022346985219513726 1106 14-15 0.0953869966541987 1106 16-17 -0.044439964016184774 1106 18-19 0.24954207996130862 1106 20-21 0.16999642776220725 1106 22-23 0.27905947409194454 1106 24-25 0.18006492465123358 1106 26-27 0.12329170791888089 1106 28-29 0.24617330654340464 1106 30-31 0.1921723136923461 1106 32-33 0.2376439872827092 1106 34-35 0.09643123037496792 1106 36-37 -0.44657284774385886 1106 38-39 -0.07839800385129081 1106 40-41 0.24982311532777857 1106 42-43 0.4276430979983985 1106 44-45 0.30278190027005536 1106 46-47 -0.19303274929073666 1106 48-49 -0.092963575473199 1106 50-51 0.09155986860168497 1106 52-53 0.0682316769241531 1106 54-55 -0.305184359871685 1106 56-57 -0.03813066093078277 1106 58-59 -0.062691468471062 1106 60-61 -0.6424098530573943 1106 62-63 -0.3676617264073343 1106 64-65 -0.9223202467945164 1106 66-67 -0.5210938099719478 1106 68-69 -1.2312071517483076 1106 70-71 -1.0664321139374344 1106 72-73 -0.8974651636611384 1106 74-75 -0.4003257336200754 1106 76-77 -0.498044067564134 1106 78-79 -0.6065617723231078 1106 80-81 -1.2632098051705576 1106 82-83 -0.7521271172518098 1106 84-85 -0.5559750819005664 1106 86-87 -0.2661050058077734 1106 88-89 -0.3464109581952073 1106 90-91 -0.49541567927258257 1106 92-93 -0.5379042156596974 1106 94-95 -0.5179396130570879 1106 96-97 -0.7388201029769998 1106 98-99 -0.9391101764396641 1106 100-101 -0.9092234430893136 1107 1 0.23956912585019907 1107 2 0.22336507996403654 1107 3 0.230019405928779 1107 4 0.19731140064369868 1107 5 0.1713727752933849 1107 6 0.22793458208155926 1107 7 0.30404768092842005 1107 8 0.15794692283424894 1107 9 0.38134342326984694 1107 10-11 0.20481390524810905 1107 12-13 0.22000526354607075 1107 14-15 0.36684864086817015 1107 16-17 0.2117329295111645 1107 18-19 0.2950479716608001 1107 20-21 0.4368403574652504 1107 22-23 0.3127904699862256 1107 24-25 0.45491864850943387 1107 26-27 0.4324049893022277 1107 28-29 0.5194047260442005 1107 30-31 0.4130618563524706 1107 32-33 0.4180240228470353 1107 34-35 0.5117904890480318 1107 36-37 0.4277956992392262 1107 38-39 0.26593091342285646 1107 40-41 0.3696110948332887 1107 42-43 0.4994467307734354 1107 44-45 0.533170718299246 1107 46-47 0.4439974121142072 1107 48-49 0.34806592802271297 1107 50-51 0.34877194805971357 1107 52-53 0.46733207942792987 1107 54-55 0.4872172873835616 1107 56-57 0.3946015545778714 1107 58-59 0.2808869980423019 1107 60-61 0.39987936002754765 1107 62-63 0.48225643839887766 1107 64-65 0.44661359921132515 1107 66-67 0.3862751435492662 1107 68-69 0.559866822673925 1107 70-71 0.5546447465951623 1107 72-73 0.4708130077241641 1107 74-75 0.37567144854314094 1107 76-77 0.22245476402667563 1107 78-79 0.42047009592129925 1107 80-81 0.6903439276873407 1107 82-83 0.8375913783709237 1107 84-85 0.7410681135511688 1107 86-87 0.8325898516401011 1107 88-89 0.44544332116165464 1107 90-91 0.4682023238493862 1107 92-93 0.22762954407317082 1107 94-95 -0.04216549929468627 1107 96-97 0.08300242916589085 1107 98-99 0.19182998176883892 1107 100-101 -0.048835773297579266 1108 1 0.18319598194764453 1108 2 0.2273021549285268 1108 3 0.22052624118753883 1108 4 0.15292305618706337 1108 5 0.18829057325795873 1108 6 0.14119526403722915 1108 7 0.15267070889144918 1108 8 0.1403628225553888 1108 9 0.08104398890866094 1108 10-11 0.17184356507362253 1108 12-13 0.14463775655622158 1108 14-15 0.4006857583133865 1108 16-17 0.323763009275126 1108 18-19 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0.8595018411820909 2316 92-93 1.0507875807273201 2316 94-95 0.9459519548004067 2316 96-97 1.2065551160659904 2316 98-99 1.096913239309261 2316 100-101 0.9306813931240878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 36.0 4 89.0 5 174.0 6 445.0 7 1149.0 8 2575.0 9 4443.0 10 7087.0 11 10542.0 12 14774.0 13 20283.0 14 26297.0 15 34008.0 16 41621.0 17 49506.0 18 47629.0 19 45157.0 20 45658.0 21 48081.0 22 51639.0 23 56493.0 24 63673.0 25 71331.0 26 82324.0 27 94639.0 28 110554.0 29 125471.0 30 142650.0 31 161380.0 32 181623.0 33 203290.0 34 224244.0 35 230488.0 36 197644.0 37 111423.0 38 21784.0 39 306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.33470697327765 20.60515241436189 16.25309276456104 21.807047847799428 2 17.677838658412202 21.995422092554378 36.76976841639191 23.556970832641507 3 20.717020635060514 24.347964571414572 30.472724944763858 24.462289848761053 4 15.377912594214862 18.08589463268207 34.64601530358002 31.890177469523053 5 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29.840970949560614 21.90135397473832 26-27 22.04772923529284 25.723832803425662 29.694287074929406 22.534150886352094 28-29 22.370964217221843 26.133196391728337 29.59596430613641 21.89987508491341 30-31 22.347352460107984 25.718162953923677 29.375436422330754 22.559048163637577 32-33 22.040083476946823 25.67941596170588 29.3696668549021 22.9108337064452 34-35 23.12677462962125 25.69650847148825 29.013372622769445 22.163344276121055 36-37 23.234624235803246 25.114991346628244 29.190542721219163 22.459841696349343 38-39 22.51864714627725 25.50383059847124 29.144104482507604 22.833417772743907 40-41 22.94822952892823 25.571964906734113 29.108994625355677 22.37081093898198 42-43 22.757476855446868 25.6607662727143 28.83838785003333 22.743369021805496 44-45 22.71978594114488 25.706327437177904 29.18338095860968 22.390505663067536 46-47 23.40636446515517 26.257755820051145 28.041698165475143 22.29418154931854 48-49 22.987300233667483 26.49219389382178 27.65618436376095 22.864321508749786 50-51 23.197770892058113 26.52700888631937 27.848022480763827 22.42719774085869 52-53 23.893280390307282 26.07536240589381 27.762289079829078 22.269068123969827 54-55 23.022905577867622 26.454138459343717 28.047210868600477 22.475745094188188 56-57 23.177043918658583 26.5717032750989 27.610521794145786 22.64073101209673 58-59 22.968667692279727 26.17301035874459 27.654188725711997 23.204133223263685 60-61 22.517752287179032 26.510865952794667 28.037746406962366 22.933635353063934 62-63 22.836580163990075 26.562021466742593 28.09874179960712 22.502656569660214 64-65 22.434449217729643 26.57407433020657 27.906725853471166 23.08475059859262 66-67 22.254009753738067 26.713197342677404 27.882721198869636 23.150071704714886 68-69 23.143899965777432 26.03187361835278 28.04711254008111 22.77711387578868 70-71 22.81230562280134 26.45610503462137 27.42080870398867 23.31078063858862 72-73 22.696272977993843 26.585494912308505 27.44717585602005 23.271056253677607 74-75 22.758157516303967 27.078417510400037 27.169248679816278 22.99417629347972 76-77 22.941064659067962 26.48776792428747 27.659009871097588 22.91215754554698 78-79 22.961100074727753 26.769548065041203 27.501314947645128 22.768036912585917 80-81 23.300455604000337 26.85994830244139 27.124344049188952 22.715252044369326 82-83 23.236371209405863 26.98109509280156 27.056890180505327 22.725643517287246 84-85 22.987491406924768 27.164926381625214 27.08327858201563 22.764303629434387 86-87 23.477268267969407 26.993841634753835 26.614661639650173 22.91422845762659 88-89 23.00896041661027 27.297844295546547 26.343039973823544 23.350155314019634 90-91 22.91322730057901 27.27716415040915 26.60001039343088 23.209598155580967 92-93 23.18794246434648 26.756841186160603 26.79661592637504 23.25860042311788 94-95 22.869305243174473 26.402257086838134 27.02837382931601 23.700063840671387 96-97 23.196407535371204 25.896505815605416 27.093651539466805 23.813435109556575 98-99 22.91814281429447 26.5315151014595 26.42501512536766 24.12532695887836 100-101 23.23879014542076 26.011857646593445 26.8216520010322 23.927700206953592 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 872.0 1 620.0 2 518.5 3 687.0 4 629.5 5 601.0 6 1095.0 7 2632.5 8 4183.5 9 4498.0 10 3637.0 11 2736.5 12 2544.5 13 2666.0 14 2866.0 15 3150.0 16 3545.0 17 3837.0 18 4183.5 19 4666.5 20 5269.5 21 6180.0 22 7394.5 23 8944.5 24 10929.5 25 13516.0 26 16606.0 27 20090.5 28 23211.0 29 26365.0 30 30237.0 31 36477.5 32 45784.5 33 46825.5 34 41761.5 35 42010.5 36 45509.0 37 49478.0 38 53313.5 39 56168.0 40 59845.5 41 66083.5 42 70347.5 43 73035.0 44 76230.0 45 81900.5 46 86391.5 47 88991.5 48 89863.0 49 87834.5 50 87213.0 51 87143.5 52 87595.5 53 90762.5 54 94914.5 55 92169.0 56 85912.5 57 80266.0 58 74143.0 59 65768.5 60 54337.5 61 45788.0 62 39835.5 63 31369.0 64 22752.0 65 17273.0 66 13290.5 67 10262.5 68 7873.0 69 6136.5 70 4847.5 71 3652.0 72 2742.0 73 2204.0 74 1751.5 75 1310.5 76 978.0 77 689.0 78 453.0 79 299.0 80 184.0 81 112.5 82 71.0 83 38.5 84 19.0 85 11.5 86 11.5 87 7.0 88 2.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.322813620447031E-4 2 0.03868771584011027 3 7.903517025558788E-4 4 0.0 5 0.07532051725357526 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.5686430333066065E-4 26-27 0.008061587366069965 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00938542646785106 36-37 9.879396281948485E-5 38-39 0.004149346438418364 40-41 0.004702592630207479 42-43 3.951758512779394E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.903517025558789E-5 68-69 9.879396281948485E-4 70-71 3.951758512779394E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012645627240894062 82-83 4.742110215335273E-4 84-85 0.005473185540199462 86-87 0.0023117787299759456 88-89 1.975879256389697E-5 90-91 0.002944060092020649 92-93 7.310753248641879E-4 94-95 1.975879256389697E-5 96-97 0.0 98-99 7.903517025558789E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2530519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.318451268811174 #Duplication Level Percentage of deduplicated Percentage of total 1 72.84322163878684 34.468284333781476 2 14.028368010903666 13.276012962097894 3 5.277414701559302 7.491572711431245 4 2.566128724242061 4.857009479501782 5 1.4072128995863484 3.3293567506959545 6 0.8774779127601073 2.4912537512638373 7 0.5915706124812905 1.9594543639129707 8 0.3959257799705206 1.4987675780480907 9 0.3113686931687146 1.3260135900903518 >10 1.5317458099784165 12.974558271275031 >50 0.10418267144799091 3.362062129325559 >100 0.05462049179671811 5.083903865130822 >500 0.005506166813930812 1.738297433105172 >1k 0.004922179424572719 4.779232344012611 >5k 2.5028030972403653E-4 0.8887684155179811 >10k+ 8.342676990801218E-5 0.4754520208092105 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12044 0.47594979527915027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9703 0.3834391284949846 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7539 0.29792307427843856 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5272 0.20833670879372967 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4844 0.19142318235903386 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4376 0.1729289525192263 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4281 0.16917478193208588 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4239 0.16751504335671852 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3913 0.1546323106050577 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3560 0.14068260305494643 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3443 0.13605904559499454 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3334 0.131751628816065 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2910 0.11499617272188037 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2814 0.11120248454961215 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 2746 0.10851528876092216 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2576 0.1017972992891972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.951758512779394E-5 7 0.0 0.0 0.0 0.0 3.951758512779394E-5 8 0.0 0.0 0.0 0.0 3.951758512779394E-5 9 0.0 0.0 0.0 0.0 3.951758512779394E-5 10-11 3.951758512779394E-5 0.0 0.0 0.0 3.951758512779394E-5 12-13 3.951758512779394E-5 0.0 0.0 0.0 1.7782913307507272E-4 14-15 3.951758512779394E-5 0.0 0.0 0.0 3.556582661501455E-4 16-17 5.927637769169092E-5 0.0 0.0 0.0 6.322813620447031E-4 18-19 7.903517025558789E-5 0.0 0.0 0.0 7.11316532300291E-4 20-21 7.903517025558789E-5 0.0 0.0 0.0 8.891456653753638E-4 22-23 7.903517025558789E-5 0.0 0.0 7.903517025558789E-5 9.879396281948485E-4 24-25 9.879396281948485E-5 0.0 0.0 3.358994735862485E-4 0.0010274572133226426 26-27 1.1855275538338183E-4 0.0 0.0 5.927637769169091E-4 0.0012645627240894062 28-29 1.1855275538338183E-4 0.0 0.0 0.0017190149530590367 0.0013040803092172 30-31 1.1855275538338183E-4 3.951758512779394E-5 0.0 0.005986914146860783 0.001383115479472788 32-33 1.1855275538338183E-4 3.951758512779394E-5 0.0 0.014838853215486626 0.0015016682348561697 34-35 1.1855275538338183E-4 3.951758512779394E-5 0.0 0.028531696462267227 0.0015807034051117577 36-37 1.1855275538338183E-4 3.951758512779394E-5 0.0 0.05512703125327255 0.0015807034051117577 38-39 1.1855275538338183E-4 3.951758512779394E-5 0.0 0.0926489783321129 0.0016202209902395517 40-41 1.5807034051117577E-4 3.951758512779394E-5 0.0 0.13418196030142432 0.0016597385753673456 42-43 1.5807034051117577E-4 3.951758512779394E-5 0.0 0.17956790682069568 0.0016794973679312426 44-45 1.7782913307507272E-4 3.951758512779394E-5 0.0 0.24247990234414363 0.0017387737456229335 46-47 1.975879256389697E-4 3.951758512779394E-5 0.0 0.3151132238090289 0.0018375677084424185 48-49 1.975879256389697E-4 3.951758512779394E-5 0.0 0.391994685675152 0.0018968440861341094 50-51 1.975879256389697E-4 3.951758512779394E-5 0.0 0.4786369910678402 0.0018968440861341094 52-53 1.975879256389697E-4 3.951758512779394E-5 0.0 0.5642320804546419 0.002094432011773079 54-55 1.975879256389697E-4 3.951758512779394E-5 0.0 0.6604574002408201 0.002193225974592564 56-57 1.975879256389697E-4 3.951758512779394E-5 0.0 0.7770540351603762 0.0022525023522842547 58-59 1.975879256389697E-4 3.951758512779394E-5 0.0 0.9020876745047163 0.0023512963151037397 60-61 1.975879256389697E-4 3.951758512779394E-5 0.0 1.0389963481799582 0.0024105726927954305 62-63 1.975879256389697E-4 3.951758512779394E-5 0.0 1.1701156956339787 0.0025291254481788123 64-65 1.975879256389697E-4 3.951758512779394E-5 0.0 1.3139794642917124 0.002548884240742709 66-67 1.975879256389697E-4 3.951758512779394E-5 0.0 1.4874221454176002 0.0025884018258705032 68-69 1.975879256389697E-4 3.951758512779394E-5 0.0 1.6821055285496769 0.0026081606184344 70-71 1.975879256389697E-4 3.951758512779394E-5 0.0 1.8870239662298525 0.002726713373817782 72-73 1.975879256389697E-4 3.951758512779394E-5 0.0 2.104963448209636 0.0028255073366372668 74-75 1.975879256389697E-4 3.951758512779394E-5 0.0 2.3276055228196273 0.0029440600920206486 76-77 1.975879256389697E-4 3.951758512779394E-5 0.0 2.57636872119909 0.0030626128474040308 78-79 1.975879256389697E-4 3.951758512779394E-5 0.0 2.85401927430697 0.0031416480176596185 80-81 1.975879256389697E-4 3.951758512779394E-5 0.0 3.144987253602917 0.0031811656027874126 82-83 2.3710551076676367E-4 3.951758512779394E-5 3.951758512779394E-5 3.4509719152474254 0.0032009243953513094 84-85 2.3710551076676367E-4 3.951758512779394E-5 3.951758512779394E-5 3.76849571174925 0.0032009243953513094 86-87 2.3710551076676367E-4 3.951758512779394E-5 3.951758512779394E-5 4.095582763852001 0.003319477150734691 88-89 2.3710551076676367E-4 3.951758512779394E-5 3.951758512779394E-5 4.437943362606643 0.003438029906118073 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5070 0.0 62.749756 1 GTATCAA 9915 0.0 45.708195 1 TGGTATC 1830 0.0 34.56165 2 TATCAAC 13100 0.0 34.268463 2 ATCAACG 13460 0.0 33.231464 3 TCAACGC 13700 0.0 32.684006 4 CAACGCA 13935 0.0 32.13282 5 AGGTAAG 1945 0.0 31.784666 2 GTGGTAT 2095 0.0 31.55184 1 TAAGGTG 2100 0.0 31.46302 5 AACGCAG 14440 0.0 31.083231 6 AAGGTAA 1900 0.0 29.283712 1 GTAAGGT 2265 0.0 27.70197 4 ACGCAGA 16415 0.0 27.19872 7 CGCAGAG 16630 0.0 26.789965 8 GGTAAGG 2415 0.0 25.587694 3 GCAGAGT 18065 0.0 24.425264 9 GTGTAAG 1015 0.0 24.363024 1 GGTGTGC 3035 0.0 23.630875 8 AAGGTGT 3070 0.0 22.897333 6 >>END_MODULE