FastQCFastQC Report
Thu 26 May 2016
SRR938337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559516
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116550.45535952891093473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.3298279831030554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65960.2577049723463342No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65540.25606403710701553No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55250.21586112374370778No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA53450.20882854414662771No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA53430.20875040437332684No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG44040.1720637808085591No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA43990.17186843137530688No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38480.1503409238309118No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA38150.1490516175714471No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35080.13705716236976054No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34770.13584599588359675No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32260.12603945433433508No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA30850.12053060031662235No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT27750.10841893545498445No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT27370.10693427976226756No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA26800.10470729622319219No Hit
TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA26730.10443380701663908No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG26600.1039258984901833No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG26560.10376961894358151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.060.50471
GTATCAA82900.047.28541
TATCAAC109950.035.4828262
ATCAACG111150.035.056993
TCAACGC112950.034.4983144
CAACGCA115100.033.8539055
AACGCAG118100.033.0705386
TGGTATC16650.029.3963382
ACGCAGA136650.028.5414777
CGCAGAG138800.028.0993718
GTGGTAT18900.027.4692291
AGGTAAG16000.027.0266152
TAAGGTG18150.026.1814465
GCAGAGT150950.025.4899719
GTAAGGT19050.023.9467474
GGTAAGG19350.023.32993
TACATGG85150.023.0481282
GTACATG86600.022.8800621
TACCGTA1252.2552189E-422.802597
AGAGTAC138950.022.66542810-11
Produced by FastQC (version 0.11.5)