FastQCFastQC Report
Thu 26 May 2016
SRR938337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559516
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120120.46930747844514353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97520.3810095346151382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77570.3030651107475007No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53640.20957087199298619No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA49530.19351314857965332No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44120.17237633990176268No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG44070.17218099046851046No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41950.16389817449861613No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA41130.16069444379327968No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35820.13994833398189344No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT34140.13338459302461872No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33150.12951667424622468No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA30490.11912408439720636No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA27950.10920033318799335No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA27300.10666079055571445No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26940.10525427463629843No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG25990.10154263540450616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49750.060.9827881
GTATCAA96500.046.1734121
TGGTATC18800.035.159012
TATCAAC130700.033.982162
ATCAACG134850.032.749793
TCAACGC136100.032.4839324
GTGGTAT22500.031.7021271
TAAGGTG21350.031.617995
CAACGCA139750.031.5674845
AACGCAG143700.030.7046246
AGGTAAG19500.029.5073642
GTAAGGT22350.027.650924
AAGGTAA19300.027.5956361
GGTAAGG22550.027.1948683
ACGCAGA163950.026.8832287
CGCAGAG165300.026.6062078
GTACCGT1951.5736077E-824.356246
GCAGAGT178950.024.0193779
GAGTACT124850.021.93085712-13
AGAGTAC165200.021.86422210-11
Produced by FastQC (version 0.11.5)