FastQCFastQC Report
Thu 26 May 2016
SRR938336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494753
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106350.42629470733174785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.31450007275269337No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA64900.2601459944130741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59200.23729804112872094No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA52750.21144377820168972No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA51280.20555141130204074No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA50470.2023045968879484No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA42050.16855376063281616No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42040.16851367650424712No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38490.15428381086223766No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA35850.1437016009200109No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33770.13536410217765044No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33530.13440208309199347No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT30490.12221650800700509No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA29840.11961103965001744No Hit
TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA27140.10878832493637647No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA26760.10726512805075293No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT26390.10578201529369842No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT25990.10417865015093679No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG25870.10369764060810831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39150.061.495241
GTATCAA76950.045.7844161
TATCAAC103750.033.718742
ATCAACG103850.033.548963
TCAACGC105100.033.285624
CAACGCA107800.032.4078455
AACGCAG111150.031.3348796
TGGTATC16200.029.6338422
GTGGTAT18850.027.5674761
ACGCAGA127850.027.1642597
CGCAGAG130350.026.6432748
GTGTAAG9100.025.6706311
TAAGGTA8450.025.312644
GCAGAGT143650.024.0753259
AGGTAAG14600.023.7657572
GTACATG83500.022.8949551
AGAGTAC130500.022.13118410-11
TACATGG82450.022.0795252
TAAGGTG17750.021.6904355
GAGTACT95150.021.56662612-13
Produced by FastQC (version 0.11.5)