FastQCFastQC Report
Thu 26 May 2016
SRR938336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494753
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116230.4658978263579601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96680.3875333550054855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74640.29918793563931984No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52200.2092391511303925No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46010.18442707554615628No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG43690.17512755771813882No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42000.16835333998997096No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40200.16113819684754363No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38050.15252010920519987No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35770.1433809278914586No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT35400.14189781513440408No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA32520.13035358610652037No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28330.11355833623609231No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA28120.11271656953614245No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA26750.10722504392218389No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25010.1002504055511708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.065.689341
GTATCAA93900.046.5035821
TGGTATC16550.035.3052332
GTGGTAT18050.035.274761
TAAGGTG20350.034.7770655
TATCAAC128100.033.9686552
ATCAACG131500.033.0857353
TCAACGC133300.032.67464
CAACGCA136150.031.955745
AACGCAG141950.030.6165876
GTAAGGT22850.029.5170674
AGGTAAG20250.029.323582
AAGGTAA19450.029.3155561
GGTAAGG23250.027.9877953
ACGCAGA163200.026.455437
CGCAGAG164500.026.2174848
TAAGGTA9550.025.3651774
GCAGAGT172550.024.5814539
AAGGTGT30050.023.393136
AGGTGTG32150.023.3424917
Produced by FastQC (version 0.11.5)