FastQCFastQC Report
Thu 26 May 2016
SRR938335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451580
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97860.399171146770654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72830.29707372388418896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54030.2203884841612348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.054.7138521
GTATCAA77800.044.741331
ATCAACG100000.034.6653863
TCAACGC101950.034.1422654
TATCAAC101400.034.092982
CAACGCA104350.033.2203035
AACGCAG108550.032.1741266
ACGCAGA125450.027.651537
CGCAGAG127300.027.2123538
GTACATG97900.025.5008091
TACATGG97500.025.0196532
GCAGAGT138200.024.5149339
GTGGTAT19950.023.59771
TGGTATC19200.023.5283182
ACATGGG99400.023.3932723
GAGTACT83900.022.4828412-13
CAGAGTA133100.021.15239310-11
GCGTTAT1353.769178E-421.134641
GTACTTT88650.021.11436714-15
AGAGTAC129550.020.96177710-11
Produced by FastQC (version 0.11.5)