##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2451580 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49816975175193 31.0 31.0 34.0 30.0 34.0 2 31.561338810073504 31.0 31.0 34.0 30.0 34.0 3 31.613308152293623 33.0 31.0 34.0 30.0 34.0 4 35.27532733991956 37.0 35.0 37.0 33.0 37.0 5 35.260273374721606 37.0 35.0 37.0 33.0 37.0 6 35.29472136336567 37.0 35.0 37.0 33.0 37.0 7 35.21688788454792 37.0 35.0 37.0 32.0 37.0 8 35.19661116504459 37.0 35.0 37.0 32.0 37.0 9 36.74457778249129 39.0 37.0 39.0 32.0 39.0 10-11 36.59454045962195 39.0 37.0 39.0 32.0 39.0 12-13 36.46150237805823 39.0 37.0 39.0 32.0 39.0 14-15 37.4861399587205 40.0 37.0 41.0 32.0 41.0 16-17 37.310689025036915 39.0 37.0 41.0 32.0 41.0 18-19 37.47609419231679 39.5 37.0 41.0 32.0 41.0 20-21 37.4639106209057 40.0 37.0 41.0 32.0 41.0 22-23 37.381670800055474 40.0 37.0 41.0 32.0 41.0 24-25 37.247814674618 39.5 37.0 41.0 31.5 41.0 26-27 37.044748284779615 39.0 36.0 41.0 31.0 41.0 28-29 36.841050057513925 39.0 36.0 41.0 30.5 41.0 30-31 36.59519738291225 39.0 36.0 40.0 30.0 41.0 32-33 36.3218864976872 39.0 35.0 40.0 30.0 41.0 34-35 36.073529111838084 38.0 35.0 40.0 29.5 41.0 36-37 35.804494448478124 38.0 35.0 40.0 28.5 41.0 38-39 35.678546896287294 38.0 35.0 40.0 27.5 41.0 40-41 35.57542095301805 38.0 35.0 40.0 27.0 41.0 42-43 35.2819569420537 38.0 34.5 40.0 26.5 41.0 44-45 34.93298077158404 38.0 34.0 40.0 25.5 41.0 46-47 34.52957888382186 38.0 33.0 40.0 24.0 41.0 48-49 34.167498511164226 38.0 33.0 40.0 23.0 41.0 50-51 33.48349982460291 37.5 32.5 39.5 22.5 40.5 52-53 33.5184583003614 37.5 33.0 39.5 22.5 40.0 54-55 33.62848550730549 38.0 33.0 40.0 22.0 41.0 56-57 33.31172366392286 37.0 32.5 40.0 19.5 41.0 58-59 32.93467824831333 37.0 32.0 40.0 18.5 41.0 60-61 32.463647117369206 36.0 31.0 39.0 15.5 41.0 62-63 31.894322233008914 35.5 31.0 39.0 10.5 40.0 64-65 31.370264278546898 35.0 30.0 38.5 7.5 40.0 66-67 30.824916584406793 35.0 29.0 38.0 4.5 40.0 68-69 30.221802062343468 34.5 29.0 37.5 2.0 40.0 70-71 29.58214008924857 34.0 28.0 37.0 2.0 39.0 72-73 28.934061707143965 34.0 26.5 36.0 2.0 39.0 74-75 28.255886815849372 33.5 26.0 36.0 2.0 39.0 76-77 27.498109178570555 33.0 25.5 35.0 2.0 37.5 78-79 26.711530115272602 32.0 24.0 35.0 2.0 37.0 80-81 26.009056200491113 32.0 21.5 35.0 2.0 36.5 82-83 25.257909796947274 31.0 19.5 34.5 2.0 36.0 84-85 24.54278077811044 31.0 16.5 34.0 2.0 35.5 86-87 23.764513293467886 30.5 8.5 34.0 2.0 35.0 88-89 22.9360171807569 30.0 2.0 34.0 2.0 35.0 90-91 21.998675955914145 29.0 2.0 34.0 2.0 35.0 92-93 21.024184199577416 29.0 2.0 34.0 2.0 35.0 94-95 19.994428083113746 27.0 2.0 33.5 2.0 35.0 96-97 18.714166170388076 25.5 2.0 33.0 2.0 34.5 98-99 17.151977500224344 21.0 2.0 32.0 2.0 34.0 100-101 13.516165697223832 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4040227698053407 1101 2 0.3215743837002094 1101 3 0.3147334204972836 1101 4 0.20549805602856708 1101 5 0.2244984355450086 1101 6 0.31266089182500423 1101 7 0.2943861421182632 1101 8 0.42052178325612743 1101 9 0.5952821631366803 1101 10-11 0.5517707439559416 1101 12-13 0.5341152859798299 1101 14-15 0.6739886139633455 1101 16-17 0.49930921379756654 1101 18-19 0.6839145703571674 1101 20-21 0.6870293625050081 1101 22-23 0.7558505174562669 1101 24-25 0.6362133748169683 1101 26-27 0.6508196005884699 1101 28-29 0.6884396470157128 1101 30-31 0.6718154501731632 1101 32-33 0.7375443683074181 1101 34-35 0.6333182546712095 1101 36-37 0.5716072641072358 1101 38-39 0.7064460945729962 1101 40-41 0.7167070589983879 1101 42-43 0.8169836007381761 1101 44-45 0.769054063996812 1101 46-47 0.8337348609604618 1101 48-49 0.6866768937907821 1101 50-51 0.8332264476517608 1101 52-53 0.8988005456116213 1101 54-55 0.9031286787807815 1101 56-57 0.8429988090701954 1101 58-59 0.8527732125139735 1101 60-61 1.0164849988942066 1101 62-63 0.9119953835485752 1101 64-65 0.8038019083755792 1101 66-67 0.8845081846351341 1101 68-69 0.9986142108842628 1101 70-71 0.8612350498529118 1101 72-73 0.8453075000832442 1101 74-75 0.8758499810265192 1101 76-77 0.9505509708430075 1101 78-79 1.2526090015639326 1101 80-81 1.2253349685261483 1101 82-83 1.1255961886841668 1101 84-85 1.214057685764086 1101 86-87 1.7154111783694965 1101 88-89 1.5434370911098974 1101 90-91 1.4415087064129004 1101 92-93 1.2744723799214164 1101 94-95 1.0197949619469462 1101 96-97 1.213195746441258 1101 98-99 1.244216782472897 1101 100-101 1.1161413646955936 1102 1 0.4008931824005444 1102 2 0.3186457178069233 1102 3 0.2796091279652586 1102 4 0.1404546412235277 1102 5 0.17631485086280918 1102 6 0.2174959179902629 1102 7 0.2463101398441836 1102 8 0.35122689351143066 1102 9 0.5492835620110341 1102 10-11 0.5826937161639165 1102 12-13 0.4524902400679025 1102 14-15 0.5820282321700461 1102 16-17 0.6388185955896475 1102 18-19 0.6580375161392666 1102 20-21 0.5846651557479774 1102 22-23 0.5966305232505533 1102 24-25 0.5544974141939534 1102 26-27 0.5506320435394869 1102 28-29 0.37410173036874284 1102 30-31 0.5888650192980336 1102 32-33 0.5647108052629122 1102 34-35 0.3907603813966176 1102 36-37 0.6486297527701694 1102 38-39 0.3883609765838827 1102 40-41 0.4556618307479141 1102 42-43 0.6007084128174682 1102 44-45 0.7069023431025485 1102 46-47 0.5917379314533022 1102 48-49 0.5275831195089751 1102 50-51 0.55099930391318 1102 52-53 0.5806425443291161 1102 54-55 0.5647482152612255 1102 56-57 0.6247180426228169 1102 58-59 0.626246633295132 1102 60-61 0.5006518989086359 1102 62-63 0.5098782526713883 1102 64-65 0.6011928683015917 1102 66-67 0.35526092207708615 1102 68-69 0.5561141290610223 1102 70-71 0.7307431952049264 1102 72-73 0.675914452755471 1102 74-75 0.6677992409201607 1102 76-77 0.6524345115224897 1102 78-79 0.7067257105968565 1102 80-81 0.812977053067133 1102 82-83 0.7401878179848858 1102 84-85 0.7353814282014959 1102 86-87 0.8844775388354158 1102 88-89 0.915112794698846 1102 90-91 0.8344451182395716 1102 92-93 0.9493781922807258 1102 94-95 1.0859801139987688 1102 96-97 1.0368015087368683 1102 98-99 1.0793220435254014 1102 100-101 1.2255275206882654 1103 1 0.3480438872686058 1103 2 0.2997302316108055 1103 3 0.23758009125475965 1103 4 0.17317015051790463 1103 5 0.22860064344340003 1103 6 0.3785725878861328 1103 7 0.23544801953669037 1103 8 0.32950948143103886 1103 9 0.4109686246509625 1103 10-11 0.45730756995430255 1103 12-13 0.5404386484107064 1103 14-15 0.5668460245998119 1103 16-17 0.6043789555306134 1103 18-19 0.6194163635360681 1103 20-21 0.5545053739760775 1103 22-23 0.6229065759656578 1103 24-25 0.7016230167750237 1103 26-27 0.6414103599137988 1103 28-29 0.666299031078708 1103 30-31 0.7877169941844713 1103 32-33 0.7670342735409506 1103 34-35 0.7541415184307638 1103 36-37 0.750554686986149 1103 38-39 0.7444038140597087 1103 40-41 0.8877275604406663 1103 42-43 0.8334960522197719 1103 44-45 0.8119350027959982 1103 46-47 0.6016563779676147 1103 48-49 0.6725417014776696 1103 50-51 0.8762630412176762 1103 52-53 1.020743084109938 1103 54-55 1.0308004523633798 1103 56-57 1.1615728314592886 1103 58-59 1.0054952065025233 1103 60-61 1.0972749213427377 1103 62-63 1.0550265936789138 1103 64-65 1.2892703062591586 1103 66-67 1.2148237949187894 1103 68-69 1.402499191908781 1103 70-71 1.3005680046962347 1103 72-73 1.2586877967690242 1103 74-75 1.3070657197328472 1103 76-77 1.3495979827813365 1103 78-79 1.2060918444497872 1103 80-81 1.3458345513473802 1103 82-83 1.379295013805585 1103 84-85 1.702523187689227 1103 86-87 1.711822346284098 1103 88-89 1.4232441691730138 1103 90-91 1.299784441484288 1103 92-93 1.1914780230815119 1103 94-95 1.307381914178631 1103 96-97 1.3890243006634009 1103 98-99 1.2325827133333895 1103 100-101 1.108587009991071 1104 1 0.38650172763641066 1104 2 0.2874628073351104 1104 3 0.3003845645991312 1104 4 0.1142871267894705 1104 5 0.23063132900185224 1104 6 0.2845235996032045 1104 7 0.32755663655636624 1104 8 0.37694651784987343 1104 9 0.5237387887763987 1104 10-11 0.4706949498519748 1104 12-13 0.5653930426097062 1104 14-15 0.6198341073440474 1104 16-17 0.6137881293328107 1104 18-19 0.6692907916488835 1104 20-21 0.7057241135941226 1104 22-23 0.7035676007367186 1104 24-25 0.690299150733729 1104 26-27 0.6682190422836811 1104 28-29 0.6678098623014179 1104 30-31 0.6949459750642504 1104 32-33 0.6734291967978763 1104 34-35 0.677691853622278 1104 36-37 0.7441266144434877 1104 38-39 0.7609759765676642 1104 40-41 0.745383622886223 1104 42-43 0.8777944115489902 1104 44-45 0.799041798209899 1104 46-47 0.7603569055402488 1104 48-49 0.5412215793213804 1104 50-51 0.7148193081178604 1104 52-53 0.7331119697117785 1104 54-55 0.7801588779940829 1104 56-57 0.8698989486463944 1104 58-59 0.8930018214045532 1104 60-61 0.921530889855589 1104 62-63 0.9404657553288054 1104 64-65 0.8845287619897597 1104 66-67 1.0872743310632522 1104 68-69 1.086832711074596 1104 70-71 1.14279301627003 1104 72-73 1.2374267319912065 1104 74-75 1.029267483035568 1104 76-77 1.1727361842254034 1104 78-79 1.2025677631424116 1104 80-81 1.29624390563227 1104 82-83 1.3123427944448558 1104 84-85 1.445362934674968 1104 86-87 1.6982972334386481 1104 88-89 1.5673870974542723 1104 90-91 1.1333096622983625 1104 92-93 1.3867836348383058 1104 94-95 1.1927795662695786 1104 96-97 1.4319602856283318 1104 98-99 1.4633196881955222 1104 100-101 1.2567432341707008 1105 1 0.2510014673876597 1105 2 0.16921114220215827 1105 3 0.23248654821016856 1105 4 0.2066690066965009 1105 5 0.17353522482324735 1105 6 0.24846053818803426 1105 7 0.2705616656780947 1105 8 0.29654865153651855 1105 9 0.334474314602474 1105 10-11 0.35119550318955106 1105 12-13 0.3132637699572882 1105 14-15 0.41718973991094543 1105 16-17 0.3415617913117046 1105 18-19 0.3763475230516917 1105 20-21 0.34311885762870276 1105 22-23 0.48246336916971444 1105 24-25 0.5290748728092538 1105 26-27 0.5508037043452063 1105 28-29 0.5200473306354638 1105 30-31 0.5125569409649486 1105 32-33 0.624291619491288 1105 34-35 0.6050246111361162 1105 36-37 0.6040850311423469 1105 38-39 0.5454554755340837 1105 40-41 0.7144720036446159 1105 42-43 0.6415557087106833 1105 44-45 0.6492291984978991 1105 46-47 0.7561593792099259 1105 48-49 0.7316613749925693 1105 50-51 0.7459457649988437 1105 52-53 0.7172139843972971 1105 54-55 0.718505136662273 1105 56-57 0.7074696165993544 1105 58-59 0.774909130474839 1105 60-61 0.7815989147123474 1105 62-63 0.8338801553830457 1105 64-65 1.0372187877180217 1105 66-67 1.075177743111535 1105 68-69 0.9765663392388078 1105 70-71 0.9775863321498655 1105 72-73 0.9786766181103062 1105 74-75 1.1060198893654416 1105 76-77 1.334076002708077 1105 78-79 1.2447608302851805 1105 80-81 1.2515525917256625 1105 82-83 1.239963343996994 1105 84-85 1.4049317904208856 1105 86-87 1.7259303540295186 1105 88-89 1.7226887917901728 1105 90-91 1.50101480505381 1105 92-93 1.6092853704185082 1105 94-95 1.6115420534425304 1105 96-97 1.8113327647996051 1105 98-99 1.8617222481674958 1105 100-101 1.6508205799583084 1106 1 0.2554380246192487 1106 2 0.06712506799494022 1106 3 0.12459061849266106 1106 4 0.0824698177691161 1106 5 0.21467763652577077 1106 6 0.017550547832101415 1106 7 0.3044982232666058 1106 8 0.1479830194002787 1106 9 -0.05862378720777173 1106 10-11 0.04040178418388507 1106 12-13 0.24185047484807143 1106 14-15 0.3998374115928627 1106 16-17 0.11228415420138305 1106 18-19 0.046242894541087765 1106 20-21 0.2940736632803791 1106 22-23 0.13177825480076422 1106 24-25 0.05014334427072953 1106 26-27 0.1895856391695716 1106 28-29 0.33694822230138044 1106 30-31 0.09292187367419302 1106 32-33 0.17633711151369624 1106 34-35 -0.09089861036837021 1106 36-37 0.032390980126578484 1106 38-39 0.17751161814923222 1106 40-41 0.5583353848080606 1106 42-43 0.050081108446988765 1106 44-45 0.385674998616544 1106 46-47 0.39788007812295234 1106 48-49 0.7000689471928041 1106 50-51 0.3452495503137314 1106 52-53 0.272401187124764 1106 54-55 0.6038489359400003 1106 56-57 -0.34917333370049874 1106 58-59 0.2781684831158273 1106 60-61 0.880798407024578 1106 62-63 0.8073995837917174 1106 64-65 0.6664358906282963 1106 66-67 0.23465459342002148 1106 68-69 0.3538171871102236 1106 70-71 0.361590383943728 1106 72-73 0.49900518746872535 1106 74-75 0.7436387537638325 1106 76-77 0.6505290983399767 1106 78-79 0.8048814989311026 1106 80-81 0.7903163234592192 1106 82-83 0.49685806802743215 1106 84-85 0.5449540338840144 1106 86-87 0.5581800204846452 1106 88-89 0.6891020904686265 1106 90-91 0.8082127354694606 1106 92-93 0.8348520040816645 1106 94-95 0.9928507628719423 1106 96-97 1.013454031649065 1106 98-99 1.2825066086429473 1106 100-101 0.5684633724980408 1107 1 0.11675736494908406 1107 2 0.07300266017229973 1107 3 0.0047434624246776025 1107 4 0.02279832608133603 1107 5 0.03317215033081311 1107 6 0.20595192236607573 1107 7 0.16186378827171666 1107 8 0.06910652844221943 1107 9 0.1369719989699334 1107 10-11 0.21172099183669957 1107 12-13 0.26033404279860406 1107 14-15 0.2868148299633191 1107 16-17 0.3613620488823628 1107 18-19 0.3008816150038598 1107 20-21 0.37597684885572846 1107 22-23 0.33023820624097766 1107 24-25 0.26032033542117716 1107 26-27 0.2744408885360414 1107 28-29 0.4333148619903824 1107 30-31 0.37074092874842535 1107 32-33 0.4798938508431476 1107 34-35 0.3095687444497983 1107 36-37 0.46573238464555544 1107 38-39 0.6077964757204484 1107 40-41 0.49210633119327696 1107 42-43 0.7115556808952803 1107 44-45 0.6400135373118019 1107 46-47 0.5108678320336111 1107 48-49 0.4144998954355046 1107 50-51 0.5282360437926599 1107 52-53 0.6013838197022778 1107 54-55 0.6201997207245995 1107 56-57 0.7011278431447039 1107 58-59 0.7007166117063974 1107 60-61 0.6549107977318798 1107 62-63 0.7071770180834172 1107 64-65 0.6215811056314173 1107 66-67 0.6350871205193052 1107 68-69 0.6638352975240451 1107 70-71 0.8340300490669215 1107 72-73 0.7831064996444965 1107 74-75 0.7004673085130086 1107 76-77 1.0666490251022616 1107 78-79 1.153936506825385 1107 80-81 1.197997991474768 1107 82-83 1.0032929037069387 1107 84-85 1.1779096799547837 1107 86-87 1.133230437753891 1107 88-89 1.173996811249829 1107 90-91 1.0967520941087265 1107 92-93 1.0370136445003943 1107 94-95 1.1364869094004497 1107 96-97 1.2462889416277 1107 98-99 0.9782019025888964 1107 100-101 0.8401311890322525 1108 1 0.18271774758962067 1108 2 0.03518970824118739 1108 3 0.13728780972005694 1108 4 0.05091922138589666 1108 5 -0.05465672284321954 1108 6 0.07718887026380372 1108 7 0.10672985081566111 1108 8 0.18145743263614378 1108 9 0.05064901002192812 1108 10-11 0.15448412354517416 1108 12-13 0.2368485510312155 1108 14-15 0.26921024729890064 1108 16-17 0.43567776712944095 1108 18-19 0.356169789500683 1108 20-21 0.18787897301488954 1108 22-23 0.09214762763647855 1108 24-25 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-1.5613476330753784 2316 56-57 -1.5222711904956725 2316 58-59 -1.3356711554404157 2316 60-61 -1.5807255947720051 2316 62-63 -1.5201349369034176 2316 64-65 -1.60114653319118 2316 66-67 -1.667007868974057 2316 68-69 -1.848284220656101 2316 70-71 -1.8949035356598714 2316 72-73 -1.654535643616768 2316 74-75 -1.8231931697023072 2316 76-77 -1.8983302587036555 2316 78-79 -1.9181284349441512 2316 80-81 -2.2644980557460066 2316 82-83 -2.2343696388659 2316 84-85 -2.291819128032241 2316 86-87 -2.4355111986934084 2316 88-89 -2.969662864856634 2316 90-91 -3.030337267419231 2316 92-93 -2.929808014023056 2316 94-95 -3.223382693446741 2316 96-97 -3.3034992422196083 2316 98-99 -3.2998151324866978 2316 100-101 -2.867726985579255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7506.0 3 2313.0 4 2326.0 5 2620.0 6 3226.0 7 3847.0 8 4934.0 9 6038.0 10 7824.0 11 10092.0 12 12655.0 13 15605.0 14 17945.0 15 21340.0 16 25365.0 17 30224.0 18 28632.0 19 26557.0 20 28366.0 21 30760.0 22 34336.0 23 38570.0 24 43602.0 25 49705.0 26 56883.0 27 64674.0 28 75062.0 29 86985.0 30 101399.0 31 119892.0 32 143559.0 33 173625.0 34 212570.0 35 258652.0 36 297905.0 37 278799.0 38 121572.0 39 5615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.676848520449376 18.488427115712526 14.305341393967577 23.529382969870525 2 15.731489968948347 19.541268483491177 40.49878631114123 24.22845523641924 3 19.663310195063012 23.704184040106156 28.839467173234034 27.7930385915968 4 12.892190952528933 15.957847780111308 36.192048558520995 34.95791270883876 5 14.087632449938845 37.06433171861991 33.727561434140036 15.120474397301203 6 33.19527180429748 35.27019573436826 17.004185938463888 14.53034652287038 7 29.51944547917752 31.936559279733302 20.07343182178891 18.470563419300273 8 26.649704460208923 35.0739043280963 19.22149021252671 19.054900999168073 9 26.68430247311995 16.621897176503033 19.654934938724818 37.038865411652196 10-11 25.372975355345794 26.35117101896402 26.89554486355456 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22.73918918312287 26.056820273319424 28.41234038784799 22.791650155709718 38-39 22.333984197685407 26.323447417185147 28.613352912986894 22.72921547214255 40-41 22.70279710221508 26.16736342090246 28.56465227131816 22.565187205564296 42-43 22.46313420369839 26.15736424398239 28.435044345857293 22.944457206461923 44-45 22.47180904680455 26.515384838176608 28.390185717834697 22.62262039718415 46-47 22.721029184990577 26.645296704551946 27.878580811501024 22.755093298956456 48-49 22.636402149252856 26.609191170546513 27.88835682672113 22.866049853479502 50-51 22.711494985277692 26.85326073210948 27.727237125833508 22.70800715677932 52-53 22.871352580905256 26.76161339545175 27.657470538046645 22.709563485596345 54-55 22.504232430822704 26.90300288417214 27.78712198783508 22.805642697170082 56-57 22.581134052140627 27.051224963401125 27.664446086699506 22.703194897758745 58-59 22.599133051149984 26.67709725126218 27.681207608751084 23.04256208883676 60-61 22.46251042935274 26.814931773163366 27.923860115095174 22.798697682388724 62-63 22.452817603964245 26.83261592824902 28.016390409786762 22.698176057999973 64-65 22.29994984430113 26.750651116428326 27.89658339542455 23.052815643845996 66-67 22.261173603976427 26.89501463787351 27.76161999129866 23.082191766851402 68-69 22.522692314831303 26.62983761234483 27.910981222199986 22.936488850623878 70-71 22.403151998488713 26.724032234011364 27.703370551793544 23.16944521570638 72-73 22.451331037077114 26.770438842797844 27.580942215781175 23.197287904343874 74-75 22.285602141076613 27.017546293396776 27.54012631601281 23.156725249513805 76-77 22.45435507146153 26.770585861750874 27.60443632271865 23.170622744068943 78-79 22.543567879086034 26.86101191860204 27.50144607585188 23.093974126460047 80-81 22.519658637052835 26.995223676272207 27.37430281541624 23.110814871258718 82-83 22.596287009264323 26.999931061124304 27.28225411359087 23.1215278160205 84-85 22.535317269549758 26.890503665881894 27.301524624238695 23.272654440329656 86-87 22.615042154773587 26.934098707014275 27.201838071738077 23.24902106647406 88-89 22.65108831901392 26.881238901365784 27.133916446870188 23.33375633275011 90-91 22.591933775080335 26.86531476769111 27.12221052762578 23.42054092960277 92-93 22.681987209669536 26.561131887940583 27.233880911366125 23.522999991023756 94-95 22.544358982782004 26.449243056599826 27.304725700981393 23.70167225963678 96-97 22.78148703107938 26.375790205612603 27.20239465149341 23.640328111814604 98-99 22.640461345730433 26.372422560800096 26.935926448085446 24.051189645384024 100-101 23.19166296922024 25.851171216328577 27.195313746867715 23.76185206758347 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1135.0 1 806.5 2 707.0 3 929.0 4 741.0 5 662.0 6 1189.5 7 2431.0 8 3764.5 9 4106.0 10 3327.5 11 2524.5 12 2400.5 13 2573.5 14 2675.0 15 2794.0 16 2978.0 17 3136.5 18 3314.5 19 3598.5 20 4072.0 21 4706.5 22 5519.5 23 6721.5 24 8205.0 25 10011.0 26 12673.0 27 15733.0 28 18617.5 29 21944.5 30 25670.0 31 30045.5 32 36186.5 33 40414.0 34 42727.5 35 46209.0 36 50827.5 37 55782.0 38 59549.5 39 62381.5 40 66059.5 41 70944.5 42 74522.5 43 78170.0 44 82773.0 45 86632.5 46 87835.5 47 89073.0 48 91701.5 49 92096.5 50 90854.0 51 88629.5 52 86694.5 53 86300.5 54 86638.0 55 82821.0 56 75932.5 57 70440.0 58 65496.0 59 58704.0 60 49067.0 61 40494.0 62 33846.0 63 26667.5 64 20060.0 65 15581.0 66 11947.5 67 9176.0 68 7137.5 69 5417.5 70 4249.0 71 3190.0 72 2344.5 73 1843.5 74 1457.0 75 1146.0 76 842.5 77 546.5 78 354.5 79 233.0 80 147.5 81 89.5 82 55.0 83 38.5 84 28.0 85 17.0 86 12.0 87 11.0 88 9.0 89 8.0 90 6.0 91 4.0 92 2.0 93 2.0 94 2.5 95 2.0 96 1.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006526403380676951 2 0.09932370144967735 3 0.10927646660520969 4 0.11180544791522201 5 0.1498625376287945 6 0.08928935625188654 7 0.024433222656409338 8 0.026146403543837037 9 0.02063975069139086 10-11 0.04690852429861559 12-13 0.05019212099951868 14-15 0.024820727857137032 16-17 0.029124075086270892 18-19 0.03436559280137707 20-21 0.054373098165264845 22-23 0.02914447009683551 24-25 0.046133513897160194 26-27 0.05345532268985716 28-29 0.049478295629757134 30-31 0.06293900260240334 32-33 0.047173659435955584 34-35 0.058492890299317174 36-37 0.04841775508039713 38-39 0.03004185056167859 40-41 0.03310110214637091 42-43 0.02981750544546782 44-45 0.007056673655356954 46-47 0.013052806761353902 48-49 0.008831039574478498 50-51 0.008076424183587727 52-53 0.009810000081580042 54-55 0.011013305704892356 56-57 0.014011372257890831 58-59 0.01855945961380008 60-61 0.023046361938015483 62-63 0.010952120673198508 64-65 0.008708669511090807 66-67 0.009524469933675426 68-69 0.010381060377389275 70-71 0.02816550958973397 72-73 0.025860873395932422 74-75 0.027064179019244728 76-77 0.02206740143091394 78-79 0.004813222493249251 80-81 0.0046092723876030965 82-83 0.005425072810187715 84-85 0.005037567609460022 86-87 0.012869251666272364 88-89 0.03377413749500322 90-91 0.031836611491364755 92-93 0.02716615407206781 94-95 0.04215648683706018 96-97 0.038832100115027864 98-99 0.02351544718100164 100-101 0.06897592572952953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2451580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.27869278167225 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12251875142744 44.74755750893441 2 12.496040948239957 14.31513780922866 3 3.930753757939811 6.754453107043143 4 1.7617857328449609 4.036511349550392 5 0.9313031911799635 2.667191468709405 6 0.5833293396127839 2.004740522052984 7 0.38858600728154913 1.5580388971235557 8 0.2888232268369919 1.3234733502565834 9 0.20630529319909793 1.0635207757545553 >10 1.173369126714508 12.26211976169089 >50 0.0802845764895515 3.1128557518781452 >100 0.032973526729335574 3.2969891732448042 >500 0.002784260702888889 1.1357345036051578 >1k 9.280869009616007E-4 0.8027509195740367 >5k 2.1417390022190788E-4 0.918925101353357 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9786 0.399171146770654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7283 0.29707372388418896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5403 0.2203884841612348 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.079002112923095E-5 0.0 5 0.0 0.0 0.0 4.079002112923095E-5 0.0 6 0.0 0.0 0.0 4.079002112923095E-5 0.0 7 0.0 0.0 0.0 4.079002112923095E-5 0.0 8 0.0 0.0 0.0 4.079002112923095E-5 0.0 9 0.0 0.0 0.0 8.15800422584619E-5 0.0 10-11 0.0 0.0 0.0 8.15800422584619E-5 0.0 12-13 0.0 0.0 0.0 1.631600845169238E-4 2.0395010564615474E-5 14-15 4.079002112923095E-5 0.0 0.0 2.243451162107702E-4 4.079002112923095E-5 16-17 4.079002112923095E-5 0.0 0.0 2.4474012677538567E-4 4.079002112923095E-5 18-19 4.079002112923095E-5 0.0 0.0 2.4474012677538567E-4 4.079002112923095E-5 20-21 4.079002112923095E-5 0.0 0.0 2.855301479046166E-4 4.079002112923095E-5 22-23 4.079002112923095E-5 0.0 0.0 4.486902324215404E-4 4.079002112923095E-5 24-25 4.079002112923095E-5 0.0 0.0 5.914553063738487E-4 4.079002112923095E-5 26-27 4.079002112923095E-5 0.0 0.0 0.001040145538795389 8.15800422584619E-5 28-29 1.427650739523083E-4 0.0 0.0 0.002284241183236933 8.15800422584619E-5 30-31 1.631600845169238E-4 0.0 0.0 0.007729709003989265 8.15800422584619E-5 32-33 1.631600845169238E-4 0.0 0.0 0.0188449897617047 8.15800422584619E-5 34-35 1.631600845169238E-4 0.0 0.0 0.03534455330847862 8.15800422584619E-5 36-37 1.631600845169238E-4 0.0 0.0 0.0670995847575849 8.15800422584619E-5 38-39 2.0395010564615472E-4 0.0 0.0 0.1169245955669405 8.15800422584619E-5 40-41 2.0395010564615472E-4 0.0 0.0 0.16813646709468993 8.15800422584619E-5 42-43 2.0395010564615472E-4 0.0 0.0 0.22124507460494863 8.15800422584619E-5 44-45 2.0395010564615472E-4 0.0 0.0 0.2895479649858459 8.15800422584619E-5 46-47 2.0395010564615472E-4 0.0 0.0 0.37396291371278934 8.15800422584619E-5 48-49 2.0395010564615472E-4 0.0 0.0 0.467963517405102 8.15800422584619E-5 50-51 2.0395010564615472E-4 0.0 0.0 0.5683885494252686 8.15800422584619E-5 52-53 2.0395010564615472E-4 0.0 0.0 0.668609631339789 8.15800422584619E-5 54-55 2.0395010564615472E-4 0.0 0.0 0.7805374493183987 8.15800422584619E-5 56-57 2.0395010564615472E-4 0.0 0.0 0.9111063069530669 8.15800422584619E-5 58-59 2.0395010564615472E-4 0.0 0.0 1.0605813393811339 8.15800422584619E-5 60-61 2.0395010564615472E-4 0.0 0.0 1.2214979727359498 8.15800422584619E-5 62-63 2.0395010564615472E-4 0.0 0.0 1.3822310509956843 8.15800422584619E-5 64-65 2.0395010564615472E-4 0.0 0.0 1.5553846906892699 8.15800422584619E-5 66-67 2.0395010564615472E-4 0.0 0.0 1.747056999975526 8.15800422584619E-5 68-69 2.0395010564615472E-4 0.0 0.0 1.9723606816828332 8.15800422584619E-5 70-71 2.243451162107702E-4 0.0 0.0 2.2108395402148817 8.15800422584619E-5 72-73 2.4474012677538567E-4 0.0 0.0 2.456701392571321 8.15800422584619E-5 74-75 2.4474012677538567E-4 0.0 0.0 2.6983210827303212 8.15800422584619E-5 76-77 2.4474012677538567E-4 0.0 0.0 2.973001085014562 8.15800422584619E-5 78-79 2.4474012677538567E-4 0.0 0.0 3.2825769503748603 8.15800422584619E-5 80-81 3.263201690338476E-4 0.0 0.0 3.6187275145008524 8.15800422584619E-5 82-83 3.263201690338476E-4 0.0 0.0 3.9607518416694543 8.15800422584619E-5 84-85 3.263201690338476E-4 0.0 0.0 4.30889467200744 8.15800422584619E-5 86-87 3.263201690338476E-4 0.0 0.0 4.660586234183669 8.15800422584619E-5 88-89 3.263201690338476E-4 0.0 0.0 5.049294740534675 8.15800422584619E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4250 0.0 54.713852 1 GTATCAA 7780 0.0 44.74133 1 ATCAACG 10000 0.0 34.665386 3 TCAACGC 10195 0.0 34.142265 4 TATCAAC 10140 0.0 34.09298 2 CAACGCA 10435 0.0 33.220303 5 AACGCAG 10855 0.0 32.174126 6 ACGCAGA 12545 0.0 27.65153 7 CGCAGAG 12730 0.0 27.212353 8 GTACATG 9790 0.0 25.500809 1 TACATGG 9750 0.0 25.019653 2 GCAGAGT 13820 0.0 24.514933 9 GTGGTAT 1995 0.0 23.5977 1 TGGTATC 1920 0.0 23.528318 2 ACATGGG 9940 0.0 23.393272 3 GAGTACT 8390 0.0 22.48284 12-13 CAGAGTA 13310 0.0 21.152393 10-11 GCGTTAT 135 3.769178E-4 21.13464 1 GTACTTT 8865 0.0 21.114367 14-15 AGAGTAC 12955 0.0 20.961777 10-11 >>END_MODULE