FastQCFastQC Report
Thu 26 May 2016
SRR938335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451580
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99090.4041883193695494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82340.3358650339780876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.25497842207882265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46300.056.652761
GTATCAA80050.041.6121061
ATCAACG109400.030.3962253
TGGTATC20150.029.9496462
TATCAAC111450.029.803012
TCAACGC112500.029.6008684
GTGGTAT21600.029.4790921
CAACGCA116800.028.5924535
AACGCAG119900.027.9193346
ACGCAGA137400.024.2942667
CGCAGAG139500.023.7923958
GTACATG93300.022.6642511
TACATGG92700.022.5033842
ACATGGG94800.021.2469433
GAGTACT94700.021.20926312-13
GCAGAGT152750.021.200129
AGTACTT100350.020.58292412-13
AGAGTAC142250.020.21143210-11
GTACTTT101250.019.7903114-15
CAGAGTA146900.019.60397710-11
Produced by FastQC (version 0.11.5)