##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938335_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2451580 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55230993889655 31.0 31.0 34.0 30.0 34.0 2 31.794516597459598 31.0 31.0 34.0 30.0 34.0 3 31.846861615774316 31.0 31.0 34.0 30.0 34.0 4 35.57025387709151 37.0 35.0 37.0 33.0 37.0 5 35.4630870703791 37.0 35.0 37.0 33.0 37.0 6 35.46888945088474 37.0 35.0 37.0 33.0 37.0 7 35.40235684742085 37.0 35.0 37.0 33.0 37.0 8 35.378680279656386 37.0 35.0 37.0 33.0 37.0 9 36.9142899681022 39.0 37.0 39.0 33.0 39.0 10-11 36.79178101469256 39.0 37.0 39.0 32.0 39.0 12-13 36.6613926529014 39.0 35.0 39.0 32.0 39.0 14-15 37.59995207172517 39.0 36.5 41.0 32.0 41.0 16-17 37.480284551187395 39.0 36.0 40.5 32.0 41.0 18-19 37.75356300834564 39.5 37.0 41.0 32.0 41.0 20-21 37.72149124238246 39.5 37.0 41.0 32.0 41.0 22-23 37.58659476745609 39.0 36.5 41.0 32.0 41.0 24-25 37.43899220094796 39.0 36.0 41.0 32.0 41.0 26-27 37.20016479168537 39.0 36.0 41.0 31.0 41.0 28-29 37.06264694605112 39.0 36.0 40.0 31.0 41.0 30-31 36.76200022026612 39.0 35.5 40.0 30.0 41.0 32-33 36.522671501643835 38.0 35.0 40.0 30.0 41.0 34-35 36.392755896197556 38.0 35.0 40.0 30.0 41.0 36-37 36.32409588918167 38.0 35.0 40.0 30.0 41.0 38-39 36.124259049266186 38.0 35.0 40.0 30.0 41.0 40-41 35.908871217745286 38.0 35.0 40.0 29.0 41.0 42-43 35.65495802706826 38.0 34.5 40.0 28.5 41.0 44-45 35.29790237316343 38.0 34.0 40.0 27.0 41.0 46-47 34.83460441837509 38.0 33.5 40.0 25.5 41.0 48-49 34.40339393370806 38.0 33.0 40.0 24.0 41.0 50-51 33.95560923975559 37.5 33.0 40.0 23.5 41.0 52-53 33.37448808523483 37.0 32.0 39.5 22.5 41.0 54-55 32.96127191443885 36.5 31.0 39.0 20.5 40.0 56-57 32.55672321523262 36.0 31.0 39.0 19.0 40.0 58-59 31.97945712560879 35.5 30.0 39.0 17.0 40.0 60-61 31.500150311227863 35.0 29.5 38.5 13.5 40.0 62-63 30.933715603814683 34.5 29.0 38.0 10.0 40.0 64-65 30.303775728305826 34.0 28.0 38.0 8.0 40.0 66-67 29.705096305239884 34.0 28.0 37.0 7.0 39.0 68-69 29.098067980649212 33.5 26.5 36.5 2.0 39.0 70-71 28.406074042046356 33.0 26.0 36.0 2.0 39.0 72-73 27.8048295385017 32.0 25.5 35.5 2.0 38.0 74-75 27.123127330129957 32.0 24.5 35.0 2.0 37.5 76-77 26.078435743479716 30.5 23.0 34.0 2.0 36.0 78-79 26.70931113812317 32.0 25.0 35.0 2.0 36.0 80-81 26.225216187111986 31.5 23.5 35.0 2.0 36.0 82-83 25.659619102782695 31.0 21.5 34.0 2.0 36.0 84-85 24.965959299716918 31.0 19.5 34.0 2.0 35.0 86-87 24.18729309261782 30.5 16.5 34.0 2.0 35.0 88-89 23.269519860661287 29.5 7.5 34.0 2.0 35.0 90-91 22.465416996386004 29.0 2.0 34.0 2.0 35.0 92-93 21.419308772301946 29.0 2.0 33.5 2.0 35.0 94-95 20.377866314784754 27.0 2.0 33.0 2.0 34.5 96-97 19.07187589228171 25.5 2.0 32.5 2.0 34.0 98-99 17.500089941996592 22.5 2.0 32.0 2.0 34.0 100-101 14.00227424762806 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19190109749117212 1101 2 0.22666620188177333 1101 3 0.2382274856983564 1101 4 0.25543899796871017 1101 5 0.14123622055316076 1101 6 0.2721616895178016 1101 7 0.20789127523507744 1101 8 0.3200827867827769 1101 9 0.3839526411681291 1101 10-11 0.3402948170507045 1101 12-13 0.36739017206114966 1101 14-15 0.34414284891376923 1101 16-17 0.42027062540923765 1101 18-19 0.42767430471702994 1101 20-21 0.4059994110918197 1101 22-23 0.4325555972328132 1101 24-25 0.4673284844595784 1101 26-27 0.4995449662112392 1101 28-29 0.50376800834524 1101 30-31 0.6588418726029417 1101 32-33 0.6211623112298241 1101 34-35 0.5740958195373267 1101 36-37 0.6494947141094016 1101 38-39 0.7985910770770062 1101 40-41 0.8913908784125368 1101 42-43 0.8574331419199979 1101 44-45 0.8620175230023293 1101 46-47 0.7676719232300542 1101 48-49 0.7307751999441905 1101 50-51 0.7635912270769367 1101 52-53 0.7327290590934794 1101 54-55 0.8876369721034081 1101 56-57 0.8131528176883478 1101 58-59 1.0089912475317782 1101 60-61 0.9792084451172691 1101 62-63 1.087720742078826 1101 64-65 0.9529139214490847 1101 66-67 0.9157196471649591 1101 68-69 1.0059742382164494 1101 70-71 1.0734704656267091 1101 72-73 1.084810699028214 1101 74-75 1.1383030988423322 1101 76-77 1.0527324896112304 1101 78-79 1.3136649918847318 1101 80-81 1.3072453201692262 1101 82-83 1.2516672700133427 1101 84-85 1.7184339089298994 1101 86-87 1.6162730512984496 1101 88-89 1.722075167589587 1101 90-91 1.7511524921008963 1101 92-93 1.9616735161296006 1101 94-95 1.9785388636230437 1101 96-97 2.0698076315503506 1101 98-99 1.9653917127872411 1101 100-101 2.003831571051407 1102 1 0.3332952927686996 1102 2 0.3075442319175714 1102 3 0.2220067859944983 1102 4 0.28476111363889345 1102 5 0.18293273754996875 1102 6 0.21408234158235473 1102 7 0.06519518129472601 1102 8 0.24848623960228622 1102 9 0.36132457567559584 1102 10-11 0.4078580845981463 1102 12-13 0.4037809065186764 1102 14-15 0.5151535720008908 1102 16-17 0.5298463385745364 1102 18-19 0.49950101764110855 1102 20-21 0.4743169676922889 1102 22-23 0.542207830279942 1102 24-25 0.4963110336838312 1102 26-27 0.5240216308292958 1102 28-29 0.5360828847800292 1102 30-31 0.6163825811800763 1102 32-33 0.5317226913260455 1102 34-35 0.5778802959110223 1102 36-37 0.674961894504797 1102 38-39 0.6591868533007386 1102 40-41 0.6672202024071083 1102 42-43 0.6465993509826191 1102 44-45 0.8279554173448886 1102 46-47 0.9591157276349307 1102 48-49 1.0012957109091118 1102 50-51 1.2157700890429552 1102 52-53 0.9658624103673148 1102 54-55 0.9779543735427652 1102 56-57 1.0729682729467953 1102 58-59 1.2119768260342134 1102 60-61 1.2126257533387452 1102 62-63 1.1281532415424245 1102 64-65 1.100002711180327 1102 66-67 1.1850153854469632 1102 68-69 1.3102966872405695 1102 70-71 1.239944544630383 1102 72-73 1.2955956990706419 1102 74-75 1.48768681832075 1102 76-77 1.4600863078472628 1102 78-79 1.6035717012691926 1102 80-81 1.5075263375412078 1102 82-83 1.6343968387503196 1102 84-85 1.748269844242266 1102 86-87 1.6470526813119406 1102 88-89 1.7615868525543554 1102 90-91 2.1191449376945 1102 92-93 2.490411353256505 1102 94-95 2.3032115100533126 1102 96-97 2.2699990979319047 1102 98-99 2.418172581665196 1102 100-101 2.224443087642129 1103 1 0.18401758843890192 1103 2 0.19701786768769125 1103 3 0.263587366117104 1103 4 0.17326212856696088 1103 5 0.09820472550562442 1103 6 0.13929430078096772 1103 7 0.27440084755335903 1103 8 0.19497294370317775 1103 9 0.3152914940974654 1103 10-11 0.2919158759709788 1103 12-13 0.4294449889313654 1103 14-15 0.6569746649880202 1103 16-17 0.5787456975532663 1103 18-19 0.5830049406342326 1103 20-21 0.5461648790068452 1103 22-23 0.5244485931282057 1103 24-25 0.5557818549101299 1103 26-27 0.6023478916810987 1103 28-29 0.606425401106911 1103 30-31 0.6072249224886406 1103 32-33 0.591841406884626 1103 34-35 0.5365687748965016 1103 36-37 0.644918568937193 1103 38-39 0.6417167897449048 1103 40-41 0.5899732953621708 1103 42-43 0.7461871450067008 1103 44-45 0.6893347546148263 1103 46-47 0.8497077586162902 1103 48-49 0.865252745769375 1103 50-51 0.7729282637340873 1103 52-53 0.728061290547025 1103 54-55 0.7397181573109819 1103 56-57 0.8713567536245677 1103 58-59 0.9452734139342276 1103 60-61 0.8444563290821812 1103 62-63 1.0493185517553343 1103 64-65 0.9530467828395679 1103 66-67 0.9830169405714955 1103 68-69 0.969927563380157 1103 70-71 1.0574653917011965 1103 72-73 1.0871706382298818 1103 74-75 1.050533904570191 1103 76-77 1.1481675311881467 1103 78-79 1.5344771738646479 1103 80-81 1.5476727769502823 1103 82-83 1.695729400558207 1103 84-85 1.5928837188151483 1103 86-87 1.5073095410945143 1103 88-89 1.5005788094312678 1103 90-91 1.619165497521518 1103 92-93 1.8884898333078297 1103 94-95 1.672794737865992 1103 96-97 1.7034633412011253 1103 98-99 1.80028646737156 1103 100-101 1.584238204883615 1104 1 0.24570137664356295 1104 2 0.2695012904915153 1104 3 0.3025065534985494 1104 4 0.25005685526262766 1104 5 0.16678289398716117 1104 6 0.09676093665225949 1104 7 0.24437141734902212 1104 8 0.23604099851563376 1104 9 0.37927895339584694 1104 10-11 0.33940660489487584 1104 12-13 0.29945251609532875 1104 14-15 0.5312754040040701 1104 16-17 0.40656149374235895 1104 18-19 0.41071896909972594 1104 20-21 0.418899635259649 1104 22-23 0.5691499380525116 1104 24-25 0.4917807536301595 1104 26-27 0.3767390617151918 1104 28-29 0.4604095090008258 1104 30-31 0.4127330877617581 1104 32-33 0.6059833999241491 1104 34-35 0.5617137191270629 1104 36-37 0.5523993793380129 1104 38-39 0.6114902438494099 1104 40-41 0.7606492212624261 1104 42-43 0.8516650498842964 1104 44-45 0.7912460473012786 1104 46-47 0.8007948348740896 1104 48-49 0.8524972741310464 1104 50-51 0.7557559269881224 1104 52-53 0.8242413460283018 1104 54-55 0.9589477194704941 1104 56-57 0.9297729589563772 1104 58-59 1.0470680567353519 1104 60-61 1.1764285525486464 1104 62-63 1.091559087466468 1104 64-65 1.1070398783919444 1104 66-67 1.1334267909284428 1104 68-69 1.2554032446877095 1104 70-71 1.2516257335707088 1104 72-73 1.150321632496894 1104 74-75 1.2461362841403094 1104 76-77 1.0416774204575674 1104 78-79 1.2497031427116134 1104 80-81 1.1232743773954645 1104 82-83 1.2446725992862753 1104 84-85 1.4202008166827262 1104 86-87 1.5445340587848122 1104 88-89 1.7142768763405911 1104 90-91 1.6863024307719634 1104 92-93 1.7022415489511573 1104 94-95 1.7716435880657961 1104 96-97 1.8921334785240838 1104 98-99 2.1772990426523187 1104 100-101 2.058477766542673 1105 1 0.22301418293021413 1105 2 0.24969978578055319 1105 3 0.26826064279388717 1105 4 0.21951276234428718 1105 5 0.22435020024661867 1105 6 0.23195101711397115 1105 7 0.26594261356810733 1105 8 0.23863769487491027 1105 9 0.30428705747839757 1105 10-11 0.30325787679444716 1105 12-13 0.44053219882490424 1105 14-15 0.5431995992280676 1105 16-17 0.40362304875159793 1105 18-19 0.3482246631446557 1105 20-21 0.3679950501498297 1105 22-23 0.33854913421226485 1105 24-25 0.3850076474606752 1105 26-27 0.34308180049253423 1105 28-29 0.2502678588189369 1105 30-31 0.4025045702897856 1105 32-33 0.5039623582652837 1105 34-35 0.6743918235556734 1105 36-37 0.586495143816073 1105 38-39 0.5556140954179298 1105 40-41 0.5804174621574276 1105 42-43 0.7589840781288828 1105 44-45 0.6477557619598926 1105 46-47 0.6169412041391737 1105 48-49 1.053734673960001 1105 50-51 0.9965809402477319 1105 52-53 1.0399802865157355 1105 54-55 1.022748071600823 1105 56-57 0.79468196370442 1105 58-59 0.9772389734253828 1105 60-61 0.876611115105451 1105 62-63 1.0341477892100954 1105 64-65 1.0575289129856067 1105 66-67 0.9797370851260574 1105 68-69 0.8464939800440412 1105 70-71 1.0843483592510843 1105 72-73 1.1726416530060497 1105 74-75 1.2404098228806468 1105 76-77 1.0375829342705245 1105 78-79 1.447202000371437 1105 80-81 1.5583935515488427 1105 82-83 1.7540131635564187 1105 84-85 1.7230658129078833 1105 86-87 1.8594191573721233 1105 88-89 2.021442439039351 1105 90-91 1.8393821587007828 1105 92-93 1.931231847357953 1105 94-95 2.217448003890336 1105 96-97 2.386038784703029 1105 98-99 2.3914106692387946 1105 100-101 2.3014130193370796 1106 1 -0.05407469312529756 1106 2 0.0537974085996602 1106 3 0.04811117757214234 1106 4 -0.015515552385011233 1106 5 -0.05667425542743132 1106 6 0.056171550057641184 1106 7 0.11649552352847792 1106 8 0.06862396760289613 1106 9 -0.2537083467765555 1106 10-11 0.04035370571679664 1106 12-13 0.07943648697340677 1106 14-15 0.1147815049274854 1106 16-17 0.03005772000020812 1106 18-19 0.13652878468133878 1106 20-21 0.12038697197505144 1106 22-23 0.09577294157073624 1106 24-25 0.27367140496409803 1106 26-27 0.1811761581768465 1106 28-29 0.23991344107156465 1106 30-31 0.23999608443715204 1106 32-33 0.23863910349553663 1106 34-35 0.22226930747448392 1106 36-37 0.3159347527423009 1106 38-39 0.2984875853644695 1106 40-41 0.35134184366280863 1106 42-43 0.49372028678912017 1106 44-45 0.5027627897806397 1106 46-47 -0.2789326905584417 1106 48-49 0.16537001822327824 1106 50-51 0.688859974356042 1106 52-53 0.6723635850728371 1106 54-55 0.6859333555083609 1106 56-57 0.6750933678337319 1106 58-59 0.5731827537147858 1106 60-61 0.7732770227151242 1106 62-63 0.582201736032804 1106 64-65 0.7695916372673146 1106 66-67 0.7336204468585912 1106 68-69 0.414832737422536 1106 70-71 0.5332767682795314 1106 72-73 0.8964781364258876 1106 74-75 1.1156342741288476 1106 76-77 1.085376184278914 1106 78-79 1.2014647716242628 1106 80-81 0.8236110856225309 1106 82-83 1.3277088407149655 1106 84-85 1.4907585390241778 1106 86-87 1.7032419920430861 1106 88-89 1.7027752051025082 1106 90-91 1.6997159819709005 1106 92-93 1.937605784529449 1106 94-95 2.1098483205527323 1106 96-97 2.119064116980823 1106 98-99 2.1492856475557005 1106 100-101 1.6022734901475317 1107 1 0.1070051651756394 1107 2 0.09595221615163751 1107 3 0.1422355755501421 1107 4 0.02278830453608549 1107 5 -0.01621439686113746 1107 6 0.08150291092587736 1107 7 0.10574868455858422 1107 8 0.10421126245104517 1107 9 0.15869496484197043 1107 10-11 0.1545206266810979 1107 12-13 0.19635479772954056 1107 14-15 0.2766126468786396 1107 16-17 0.19485751037051102 1107 18-19 0.23542623860924294 1107 20-21 0.23594036784349015 1107 22-23 0.3111259951324499 1107 24-25 0.2811228783424511 1107 26-27 0.12897438295826902 1107 28-29 0.25017766736549873 1107 30-31 0.1945276682027881 1107 32-33 0.13585918814347764 1107 34-35 0.226823446334258 1107 36-37 0.322812397990468 1107 38-39 0.4108550741864292 1107 40-41 0.27632957103805467 1107 42-43 0.30853798855770975 1107 44-45 0.3062249967302577 1107 46-47 0.39269333271310813 1107 48-49 0.41949627367961995 1107 50-51 0.5314221003726871 1107 52-53 0.5826805504839356 1107 54-55 0.4293326735484655 1107 56-57 0.38313425889262476 1107 58-59 0.40995684454548 1107 60-61 0.6444078289934048 1107 62-63 0.7251678635884922 1107 64-65 0.5291410660927269 1107 66-67 0.4969677555051568 1107 68-69 0.7743426948332122 1107 70-71 0.7271096084965905 1107 72-73 0.73653266668288 1107 74-75 0.8974021291393939 1107 76-77 0.9155396423728774 1107 78-79 1.193283574612149 1107 80-81 1.1133528297946924 1107 82-83 1.3747826489435937 1107 84-85 1.5599180964136252 1107 86-87 1.7570292577022926 1107 88-89 1.6708000422414635 1107 90-91 1.7797467630405421 1107 92-93 1.8938688034624604 1107 94-95 1.9445626005808592 1107 96-97 2.077090081874484 1107 98-99 1.9893650547547566 1107 100-101 1.8455932019730348 1108 1 0.18948052041837116 1108 2 0.19385127547153402 1108 3 0.18162406714505508 1108 4 0.18533092703015086 1108 5 0.1614865756614492 1108 6 0.1608271868410185 1108 7 0.1199583938632216 1108 8 0.06785444086183645 1108 9 0.1406741080137479 1108 10-11 0.20426290156134996 1108 12-13 0.20852459778525656 1108 14-15 0.280229754254151 1108 16-17 0.33459792777242825 1108 18-19 0.35276579891758075 1108 20-21 0.3752927049492669 1108 22-23 0.19754285307516994 1108 24-25 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quality scores pass #Quality Count 2 5.0 3 25.0 4 45.0 5 120.0 6 293.0 7 666.0 8 1541.0 9 2754.0 10 4352.0 11 6749.0 12 9222.0 13 13285.0 14 17415.0 15 22805.0 16 27936.0 17 34032.0 18 33193.0 19 30776.0 20 31472.0 21 33588.0 22 36599.0 23 41163.0 24 46402.0 25 52967.0 26 62290.0 27 72831.0 28 85660.0 29 99412.0 30 115258.0 31 133818.0 32 157076.0 33 185356.0 34 222473.0 35 265103.0 36 292057.0 37 240769.0 38 70737.0 39 1335.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.94102737094231 19.941604958112695 15.506869453062478 22.610498217882522 2 16.61043951851007 20.869732709182742 38.95817018482524 23.561657587481946 3 19.92364108044608 24.38092984932166 29.754444072802027 25.940984997430228 4 13.821785877591545 17.286450895524887 35.817283473222766 33.0744797536608 5 13.93759589957886 37.4138536123535 33.89907283470993 14.74947765335771 6 32.932109088832505 36.23267443852536 16.55454849525612 14.280667977386013 7 29.148753049054076 32.35244209856501 20.03507941817114 18.463725434209774 8 26.54508521035414 35.87070379102457 18.910457745617112 18.673753253004186 9 26.35410633142708 16.712446667047374 19.559875672015597 37.37357132950995 10-11 25.40426989941181 26.7466694947748 26.779709411889474 21.069351193923918 12-13 26.244218014504934 23.789882443159104 27.43143197448176 22.5344675678542 14-15 22.76915295442123 25.267970043808486 25.739829008231425 26.223047993538863 16-17 22.406305321466156 28.51501888577978 26.42807903474494 22.65059675800912 18-19 22.364638314882647 26.794924089770678 29.000624087323278 21.839813508023397 20-21 22.869700356504786 25.901683812072214 29.391922760015987 21.836693071407012 22-23 22.637747901353414 25.895748863997913 29.189848995341777 22.276654239306897 24-25 22.540043456259518 26.064373434205052 29.219368038375958 22.176215071159472 26-27 22.140273331571166 26.502042807587756 29.076579994191377 22.281103866649698 28-29 22.24930004209537 26.628931756551705 29.08690165020379 22.034866551149136 30-31 22.375259377038255 26.278672201675107 29.00984561992897 22.336222801357675 32-33 22.127849379447287 26.299206078404218 29.02483291791833 22.54811162423016 34-35 22.68064283863967 26.344349869355838 28.890085525524057 22.084921766480438 36-37 22.765203835360133 26.065544977029088 28.78611051782421 22.383140669786574 38-39 22.311653659868785 26.323320923795045 28.91786147479813 22.447163941538044 40-41 22.70983200223695 26.14757476951831 28.84751915165525 22.29507407658949 42-43 22.484129889651108 26.19458973279068 28.663484316506853 22.657796061051357 44-45 22.513310804636713 26.498842290421116 28.649293952170847 22.338552952771323 46-47 22.798521769634277 26.667638828836914 28.03547100237398 22.49836839915483 48-49 22.710802829195867 26.68277192667586 27.99592507688919 22.610500167239085 50-51 22.81116061458638 26.870502239893458 27.83205031066858 22.48628683485158 52-53 23.019134598911723 26.78692924562935 27.706071186744875 22.487864968714053 54-55 22.64687916509878 26.90290520579348 27.809393862448108 22.64082176665963 56-57 22.732217590288712 27.007990765139215 27.734379461408558 22.52541218316351 58-59 22.72339033157551 26.72436461168226 27.736608187420813 22.815636869321416 60-61 22.593715073544406 26.892881325512523 27.87033260183229 22.64307099911078 62-63 22.56186759697866 26.947833940614284 27.962004010828256 22.528294451578795 64-65 22.3958410888299 26.77747822154573 27.91144485895484 22.915235830669523 66-67 22.352300493997383 26.922265779565397 27.75716622542761 22.968267501009613 68-69 22.625142328886103 26.721378889999915 27.814501452152406 22.838977328961576 70-71 22.47850618632289 26.803394956920595 27.660659310178854 23.057439546577662 72-73 22.529983245481734 26.877265760515424 27.46374528237431 23.129005711628533 74-75 22.47036641063474 27.092180265570654 27.338196124413848 23.09925719938076 76-77 22.468966769677166 26.86664102456846 27.65804323584235 23.006348969912025 78-79 22.53826853019426 26.93611231759805 27.584861140210737 22.940758011996955 80-81 22.502672422234554 27.112498775989817 27.396203936416754 22.98862486535888 82-83 22.56769844367384 27.097433244128965 27.388304632132467 22.94656368006473 84-85 22.506476915134208 27.01527880873309 27.319047709382176 23.159196566750524 86-87 22.527710343032652 27.07360991392058 27.21679684711348 23.18188289593329 88-89 22.4408879065988 27.15554694645605 27.0436513322865 23.359913814658654 90-91 22.392924951024682 27.068076252779754 27.255450320983915 23.283548475211646 92-93 22.425140258105106 26.89926263261332 27.20611484946861 23.469482259812967 94-95 22.238565004857275 26.796508148021818 27.274014336405735 23.690912510715176 96-97 22.410742594033607 26.66470259636764 27.15868531880574 23.765869490793012 98-99 22.24729345008391 26.802434915473366 26.873037016722495 24.07723461772023 100-101 22.40064783141697 26.62940004201929 27.200087302218662 23.76986482434508 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1225.0 1 851.5 2 686.5 3 909.0 4 738.5 5 645.0 6 1145.0 7 2560.5 8 3938.0 9 4183.0 10 3390.5 11 2539.0 12 2353.5 13 2469.5 14 2716.0 15 2891.5 16 3026.0 17 3213.0 18 3481.0 19 3866.5 20 4359.0 21 5054.5 22 5868.0 23 7025.0 24 8643.0 25 10582.0 26 13137.0 27 16194.0 28 19298.5 29 22835.5 30 26809.0 31 31289.0 32 37326.0 33 41463.0 34 43686.0 35 46962.0 36 51767.5 37 56896.5 38 60586.0 39 63433.5 40 66752.0 41 71601.0 42 75584.0 43 79001.0 44 82963.0 45 86798.5 46 88209.0 47 90144.0 48 92439.5 49 92305.0 50 91357.0 51 88803.5 52 86530.5 53 85781.5 54 85975.0 55 81956.0 56 75018.5 57 69050.5 58 63345.5 59 56121.5 60 46781.5 61 38902.0 62 32605.0 63 25621.5 64 18949.0 65 14322.0 66 10878.5 67 8413.5 68 6462.5 69 4876.5 70 3777.5 71 2765.5 72 2069.5 73 1686.0 74 1325.5 75 1021.0 76 731.0 77 459.0 78 293.0 79 192.5 80 112.0 81 66.0 82 44.0 83 27.5 84 11.5 85 7.0 86 6.5 87 2.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.15800422584619E-5 2 0.04499139330554173 3 0.0 4 4.079002112923095E-5 5 0.04486902324215404 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006098108158820026 26-27 0.002121081098720009 28-29 1.631600845169238E-4 30-31 0.0013460706972646213 32-33 6.322453275030797E-4 34-35 0.010809355599246201 36-37 0.02225095652599548 38-39 0.0043033472291338646 40-41 0.0012033056233123128 42-43 0.0015296257923461604 44-45 0.007668523972295418 46-47 0.0 48-49 0.0 50-51 5.098752641153868E-4 52-53 0.0 54-55 0.0013256756867000058 56-57 0.0 58-59 0.022006216399220097 60-61 0.0 62-63 0.008810644563913884 64-65 0.014745592638216988 66-67 0.006118503169384642 68-69 0.0159488982615293 70-71 1.0197505282307736E-4 72-73 1.0197505282307736E-4 74-75 0.021149625955506245 76-77 0.04823419998531559 78-79 0.028899729970060125 80-81 0.02528981310012319 82-83 0.03428401275911861 84-85 0.015275862912896988 86-87 0.009748815049886197 88-89 0.08058068674079574 90-91 0.024494407688103185 92-93 0.02296478189575702 94-95 0.025167443036735494 96-97 0.03618074874162785 98-99 0.0500901459466956 100-101 0.013256756867000057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2451580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.13728354299763 #Duplication Level Percentage of deduplicated Percentage of total 1 77.31696920911028 43.403646231770416 2 12.866063967892705 14.445317620958756 3 4.113487339648924 6.927600154092079 4 1.8118129852311315 4.0684103711522 5 1.0012516027971967 2.8103772562053533 6 0.5997600514869733 2.0201340040852247 7 0.418448575453322 1.64433964298706 8 0.29366322816158735 1.318836472436723 9 0.22310433463055226 1.1272024163544423 >10 1.2337079957279808 12.685879490851315 >50 0.08302098985322745 3.15993733663607 >100 0.034852152935972575 3.4966782155041405 >500 0.0026202342363879066 1.069610045375183 >1k 0.0010189799808162918 0.8253133664342043 >5k 2.1835285303206252E-4 0.9967173751569868 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9909 0.4041883193695494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8234 0.3358650339780876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6251 0.25497842207882265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.079002112923095E-5 2 0.0 0.0 0.0 0.0 4.079002112923095E-5 3 0.0 0.0 0.0 0.0 4.079002112923095E-5 4 0.0 0.0 0.0 0.0 4.079002112923095E-5 5 0.0 0.0 0.0 0.0 4.079002112923095E-5 6 0.0 0.0 0.0 0.0 8.15800422584619E-5 7 0.0 0.0 0.0 0.0 8.15800422584619E-5 8 0.0 0.0 0.0 0.0 8.15800422584619E-5 9 0.0 0.0 0.0 0.0 8.15800422584619E-5 10-11 2.0395010564615474E-5 2.0395010564615474E-5 0.0 0.0 8.15800422584619E-5 12-13 4.079002112923095E-5 4.079002112923095E-5 0.0 0.0 8.15800422584619E-5 14-15 4.079002112923095E-5 4.079002112923095E-5 0.0 6.118503169384642E-5 8.15800422584619E-5 16-17 4.079002112923095E-5 4.079002112923095E-5 0.0 8.15800422584619E-5 8.15800422584619E-5 18-19 4.079002112923095E-5 4.079002112923095E-5 0.0 8.15800422584619E-5 8.15800422584619E-5 20-21 4.079002112923095E-5 4.079002112923095E-5 0.0 8.15800422584619E-5 8.15800422584619E-5 22-23 4.079002112923095E-5 4.079002112923095E-5 0.0 2.243451162107702E-4 8.15800422584619E-5 24-25 4.079002112923095E-5 4.079002112923095E-5 0.0 3.4671517959846304E-4 8.15800422584619E-5 26-27 4.079002112923095E-5 4.079002112923095E-5 0.0 7.342203803261569E-4 8.15800422584619E-5 28-29 4.079002112923095E-5 4.079002112923095E-5 0.0 0.0020395010564615472 8.15800422584619E-5 30-31 4.079002112923095E-5 4.079002112923095E-5 0.0 0.007423783845520032 8.15800422584619E-5 32-33 4.079002112923095E-5 4.079002112923095E-5 0.0 0.018437089550412386 8.15800422584619E-5 34-35 8.15800422584619E-5 4.079002112923095E-5 0.0 0.03509981318170323 8.15800422584619E-5 36-37 1.2237006338769284E-4 4.079002112923095E-5 0.0 0.06679365959911567 8.15800422584619E-5 38-39 1.631600845169238E-4 4.079002112923095E-5 0.0 0.1162515602183082 8.15800422584619E-5 40-41 1.631600845169238E-4 4.079002112923095E-5 0.0 0.16723908662984688 8.15800422584619E-5 42-43 2.0395010564615474E-4 4.079002112923095E-5 0.0 0.22008255900276558 8.15800422584619E-5 44-45 2.4474012677538567E-4 4.079002112923095E-5 0.0 0.2878347840984181 8.15800422584619E-5 46-47 2.4474012677538567E-4 4.079002112923095E-5 0.0 0.371433932402777 8.15800422584619E-5 48-49 3.263201690338476E-4 4.079002112923095E-5 0.0 0.46396609533443733 8.15800422584619E-5 50-51 3.263201690338476E-4 4.079002112923095E-5 0.0 0.5625759714143532 8.15800422584619E-5 52-53 3.263201690338476E-4 4.079002112923095E-5 0.0 0.6611246624625752 1.2237006338769284E-4 54-55 3.263201690338476E-4 4.079002112923095E-5 0.0 0.7713800895748864 1.2237006338769284E-4 56-57 3.263201690338476E-4 4.079002112923095E-5 0.0 0.8989916706776854 1.2237006338769284E-4 58-59 3.263201690338476E-4 4.079002112923095E-5 0.0 1.0443673059822645 1.2237006338769284E-4 60-61 3.263201690338476E-4 4.079002112923095E-5 0.0 1.2011437521924635 1.2237006338769284E-4 62-63 3.263201690338476E-4 4.079002112923095E-5 0.0 1.3575734832230644 1.2237006338769284E-4 64-65 3.263201690338476E-4 4.079002112923095E-5 0.0 1.526260615602999 1.2237006338769284E-4 66-67 3.263201690338476E-4 4.079002112923095E-5 0.0 1.713955897829155 1.2237006338769284E-4 68-69 3.263201690338476E-4 4.079002112923095E-5 0.0 1.9351397874024099 1.2237006338769284E-4 70-71 3.671101901630785E-4 4.079002112923095E-5 0.0 2.1690705585785492 1.2237006338769284E-4 72-73 4.0790021129230944E-4 4.079002112923095E-5 0.0 2.4114652591390042 1.2237006338769284E-4 74-75 4.0790021129230944E-4 4.079002112923095E-5 0.0 2.650739523083073 1.2237006338769284E-4 76-77 4.0790021129230944E-4 4.079002112923095E-5 0.0 2.922013558603023 1.2237006338769284E-4 78-79 4.0790021129230944E-4 4.079002112923095E-5 0.0 3.22636830125878 1.2237006338769284E-4 80-81 4.486902324215404E-4 4.079002112923095E-5 0.0 3.5577464329126522 1.2237006338769284E-4 82-83 4.486902324215404E-4 4.079002112923095E-5 0.0 3.8937950219858215 1.2237006338769284E-4 84-85 4.486902324215404E-4 4.079002112923095E-5 0.0 4.235819349154423 1.2237006338769284E-4 86-87 4.486902324215404E-4 4.079002112923095E-5 0.0 4.5816983333197365 1.2237006338769284E-4 88-89 4.486902324215404E-4 4.079002112923095E-5 0.0 4.960250124409564 1.2237006338769284E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4630 0.0 56.65276 1 GTATCAA 8005 0.0 41.612106 1 ATCAACG 10940 0.0 30.396225 3 TGGTATC 2015 0.0 29.949646 2 TATCAAC 11145 0.0 29.80301 2 TCAACGC 11250 0.0 29.600868 4 GTGGTAT 2160 0.0 29.479092 1 CAACGCA 11680 0.0 28.592453 5 AACGCAG 11990 0.0 27.919334 6 ACGCAGA 13740 0.0 24.294266 7 CGCAGAG 13950 0.0 23.792395 8 GTACATG 9330 0.0 22.664251 1 TACATGG 9270 0.0 22.503384 2 ACATGGG 9480 0.0 21.246943 3 GAGTACT 9470 0.0 21.209263 12-13 GCAGAGT 15275 0.0 21.20012 9 AGTACTT 10035 0.0 20.582924 12-13 AGAGTAC 14225 0.0 20.211432 10-11 GTACTTT 10125 0.0 19.79031 14-15 CAGAGTA 14690 0.0 19.603977 10-11 >>END_MODULE