FastQCFastQC Report
Thu 26 May 2016
SRR938334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493691
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99800.40020996987998914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75250.30176152538546275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55270.2216393290106914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.056.5284541
GTATCAA74900.045.3725741
ATCAACG98550.034.2413333
TCAACGC100550.033.6547854
TATCAAC100950.033.333112
CAACGCA103250.032.6346475
AACGCAG106500.031.85746
ACGCAGA124000.027.3975187
GTGGTAT19250.026.950871
GTACATG98900.026.8543661
CGCAGAG125500.026.8428978
TACATGG96550.026.5821672
TGGTATC18250.024.7405662
ACATGGG100600.024.2364083
GCAGAGT136450.024.0953529
CATGGGG51550.021.6664684
TAAGGTG10450.021.3749125
GAGTACT81750.021.23776812-13
AGAGTAC126750.021.0821210-11
CAGAGTA131050.020.60787410-11
Produced by FastQC (version 0.11.5)