##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938334_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2493691 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.374064388891806 31.0 31.0 34.0 30.0 34.0 2 31.488963949422764 31.0 31.0 34.0 30.0 34.0 3 31.56096926202966 33.0 31.0 34.0 28.0 34.0 4 35.20120856994712 37.0 35.0 37.0 33.0 37.0 5 35.22092071551768 37.0 35.0 37.0 33.0 37.0 6 35.26576949589985 37.0 35.0 37.0 32.0 37.0 7 35.19473543434211 37.0 35.0 37.0 32.0 37.0 8 35.16109534020053 37.0 35.0 37.0 32.0 37.0 9 36.71436396891195 39.0 37.0 39.0 32.0 39.0 10-11 36.57249635179339 39.0 37.0 39.0 32.0 39.0 12-13 36.43389918799082 39.0 37.0 39.0 32.0 39.0 14-15 37.47388329187538 40.0 37.0 41.0 32.0 41.0 16-17 37.319931378827604 39.0 37.0 41.0 32.0 41.0 18-19 37.318076096837984 39.5 36.5 41.0 32.0 41.0 20-21 37.418488297066475 40.0 37.0 41.0 32.0 41.0 22-23 37.34297252546527 40.0 37.0 41.0 32.0 41.0 24-25 37.23277603359839 39.5 36.5 41.0 31.0 41.0 26-27 37.044785420487145 39.0 36.0 41.0 31.0 41.0 28-29 36.861688557243056 39.0 36.0 41.0 30.5 41.0 30-31 36.64379488076109 39.0 36.0 40.5 30.0 41.0 32-33 36.36806946008949 39.0 35.0 40.0 30.0 41.0 34-35 36.116607069600846 38.5 35.0 40.0 29.5 41.0 36-37 35.838049702228545 38.0 35.0 40.0 28.5 41.0 38-39 35.5818208831808 38.0 35.0 40.0 27.0 41.0 40-41 35.5181925106198 38.0 35.0 40.0 27.0 41.0 42-43 35.339213639540745 38.0 34.5 40.0 26.5 41.0 44-45 35.041392056995036 38.0 34.0 40.0 25.5 41.0 46-47 34.669309669882914 38.0 33.5 40.0 24.0 41.0 48-49 34.29400094077414 38.0 33.0 40.0 23.5 41.0 50-51 33.55967138671151 37.5 32.5 39.5 22.5 40.5 52-53 33.64114198591565 37.5 33.0 39.5 23.0 40.5 54-55 33.771560109091304 38.0 33.0 40.0 22.5 41.0 56-57 33.47215152157986 37.5 33.0 40.0 20.5 41.0 58-59 33.13912569761049 37.0 32.0 40.0 19.5 41.0 60-61 32.690906772330656 36.5 31.5 39.0 17.5 41.0 62-63 32.16040800564304 36.0 31.0 39.0 13.5 40.5 64-65 31.664323286245168 35.0 30.5 39.0 8.5 40.0 66-67 31.119167130169693 35.0 30.0 38.0 7.5 40.0 68-69 30.540195437205334 35.0 29.0 38.0 4.0 40.0 70-71 29.92232016717388 34.0 28.5 37.0 2.0 39.0 72-73 29.364571432466974 34.0 28.0 36.5 2.0 39.0 74-75 28.740976528366986 34.0 26.5 36.0 2.0 39.0 76-77 28.00132494362774 33.5 26.0 35.5 2.0 38.0 78-79 27.229502572692446 33.0 25.0 35.0 2.0 37.0 80-81 26.57498563374532 32.0 24.0 35.0 2.0 36.5 82-83 25.916584292119595 32.0 21.5 35.0 2.0 36.0 84-85 25.260831634713362 31.0 19.5 34.5 2.0 35.5 86-87 24.580305258349973 31.0 17.5 34.0 2.0 35.0 88-89 23.78546540048466 30.5 9.0 34.0 2.0 35.0 90-91 22.942413875656605 30.0 2.0 34.0 2.0 35.0 92-93 22.087578212376755 29.0 2.0 34.0 2.0 35.0 94-95 21.11799517261762 29.0 2.0 34.0 2.0 35.0 96-97 19.867067531622805 27.5 2.0 33.5 2.0 35.0 98-99 18.373219657126725 25.0 2.0 33.0 2.0 35.0 100-101 14.875165968838964 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4698990013317683 1101 2 0.44561358560092756 1101 3 0.4875099587817253 1101 4 0.41434842008441564 1101 5 0.35726584780097426 1101 6 0.3983392647862729 1101 7 0.4222266884210839 1101 8 0.43572381286716677 1101 9 0.40641038093670545 1101 10-11 0.47336116400572337 1101 12-13 0.5300013124229537 1101 14-15 0.6356183269212679 1101 16-17 0.6418080264686097 1101 18-19 0.5645167233372419 1101 20-21 0.6704568842676082 1101 22-23 0.6876345430066948 1101 24-25 0.7334881470493144 1101 26-27 0.8232274420335202 1101 28-29 0.8610761177590476 1101 30-31 0.7800147698829036 1101 32-33 0.7748546136618017 1101 34-35 0.7328813045364342 1101 36-37 0.7576414131470202 1101 38-39 0.6968019360084696 1101 40-41 0.6782583207391113 1101 42-43 0.7935897206342091 1101 44-45 0.7516543479108364 1101 46-47 0.8010667361732331 1101 48-49 0.8902683568740883 1101 50-51 0.760267572342542 1101 52-53 0.7711357338498459 1101 54-55 0.8332484869702341 1101 56-57 0.8283297420554945 1101 58-59 0.8167909022834365 1101 60-61 0.6882210764481655 1101 62-63 0.7504732091030135 1101 64-65 0.736603070437102 1101 66-67 0.7762328837969967 1101 68-69 0.7244513257773413 1101 70-71 0.7511134564914457 1101 72-73 0.8515896029019991 1101 74-75 0.64820662225803 1101 76-77 0.7996833240544596 1101 78-79 0.7462333191936885 1101 80-81 0.9439128800366241 1101 82-83 1.0466302749968968 1101 84-85 1.15337411148721 1101 86-87 1.4161262628974676 1101 88-89 1.4706317308448114 1101 90-91 1.3661289850685314 1101 92-93 1.3791488010441029 1101 94-95 1.2490282775380983 1101 96-97 1.2298321732855086 1101 98-99 1.4380976536742516 1101 100-101 1.2673690101438577 1102 1 0.4927860184853188 1102 2 0.5144721678668382 1102 3 0.5268660322273782 1102 4 0.44650386907014905 1102 5 0.3886252058676334 1102 6 0.3822960037496088 1102 7 0.48775589194823255 1102 8 0.4057086787849258 1102 9 0.4852394947096741 1102 10-11 0.645140606240787 1102 12-13 0.5580678439013695 1102 14-15 0.6979642111192774 1102 16-17 0.6211958958094428 1102 18-19 0.6813002474777718 1102 20-21 0.534615814808383 1102 22-23 0.6138063601946158 1102 24-25 0.6192553662452482 1102 26-27 0.5606290346927736 1102 28-29 0.3571508643705883 1102 30-31 0.40450050350396083 1102 32-33 0.5168604345544736 1102 34-35 0.6870061177474867 1102 36-37 0.6018682322207383 1102 38-39 0.6425624236410101 1102 40-41 0.6237016252206118 1102 42-43 0.6763865181287301 1102 44-45 0.6241805878791311 1102 46-47 0.5903873018155181 1102 48-49 0.4681233931338227 1102 50-51 0.5742650169742376 1102 52-53 0.6275778340498874 1102 54-55 0.6145868089012936 1102 56-57 0.5561112562753223 1102 58-59 0.6680548308906751 1102 60-61 0.7792708422757002 1102 62-63 0.45327871346898263 1102 64-65 0.48180011785881405 1102 66-67 0.6653906506366454 1102 68-69 0.4876056241908877 1102 70-71 0.37531020433471696 1102 72-73 0.579793630143346 1102 74-75 0.5886768947669978 1102 76-77 0.6656861240983822 1102 78-79 0.7244063144052078 1102 80-81 0.8398688992955776 1102 82-83 0.7692828511327896 1102 84-85 0.6833559756528551 1102 86-87 0.7427744814202484 1102 88-89 0.8717157930329975 1102 90-91 0.9146214550271416 1102 92-93 0.9642187842390655 1102 94-95 1.162698662478494 1102 96-97 1.2518476081397338 1102 98-99 1.3507251833814529 1102 100-101 0.9036287136208774 1103 1 0.6017456968284876 1103 2 0.551829447075356 1103 3 0.5590317155776354 1103 4 0.6062444729112428 1103 5 0.5412660232779984 1103 6 0.4945526413175756 1103 7 0.43991762862463446 1103 8 0.4700713798605278 1103 9 0.5365686989010783 1103 10-11 0.619268177080528 1103 12-13 0.6290106180050117 1103 14-15 0.7805406371175465 1103 16-17 0.7657673829272298 1103 18-19 0.6749002378005571 1103 20-21 0.5971077079497036 1103 22-23 0.852884144355869 1103 24-25 0.7604672982586962 1103 26-27 0.7955058993431336 1103 28-29 0.7681452804392066 1103 30-31 0.9223476162380422 1103 32-33 1.056207956675813 1103 34-35 1.0073861119003524 1103 36-37 0.9304611846971298 1103 38-39 0.7502498534847959 1103 40-41 0.9409497850183399 1103 42-43 0.8391801831633856 1103 44-45 0.8917043735846519 1103 46-47 0.9123971935676352 1103 48-49 0.861371986657602 1103 50-51 0.8769838642656538 1103 52-53 0.8520800328485691 1103 54-55 0.9135419605203197 1103 56-57 0.9570152748579162 1103 58-59 0.8133752095731523 1103 60-61 0.8588710127347028 1103 62-63 1.0462010942868716 1103 64-65 0.7489446847550845 1103 66-67 0.9503876498236998 1103 68-69 0.8380693146457361 1103 70-71 1.1259020596707572 1103 72-73 1.1285214253663547 1103 74-75 0.9698909750068978 1103 76-77 0.921612328875959 1103 78-79 1.016362168205717 1103 80-81 0.8008151187230688 1103 82-83 0.9087165937950807 1103 84-85 0.9984892108731565 1103 86-87 1.0077843029250744 1103 88-89 0.9587645816752044 1103 90-91 1.1032621173687893 1103 92-93 1.1913768263740323 1103 94-95 1.1105199323688986 1103 96-97 1.1367242396040496 1103 98-99 1.0419827032671236 1103 100-101 0.9928424889700214 1104 1 0.346935947790179 1104 2 0.4152858136219173 1104 3 0.35081572717538734 1104 4 0.4433632787063502 1104 5 0.36472294993674126 1104 6 0.4035262671780657 1104 7 0.22272441364071938 1104 8 0.26831682844894544 1104 9 0.42537800042118334 1104 10-11 0.33127763954715306 1104 12-13 0.34408848074266274 1104 14-15 0.5341382862466943 1104 16-17 0.5105351206941364 1104 18-19 0.5907458828854715 1104 20-21 0.5587856754511975 1104 22-23 0.5910622766046387 1104 24-25 0.6213174285276395 1104 26-27 0.5674462936906863 1104 28-29 0.5931256550799517 1104 30-31 0.6371118447306685 1104 32-33 0.5930902722481619 1104 34-35 0.5902280611021737 1104 36-37 0.5860266993902101 1104 38-39 0.5630816520821753 1104 40-41 0.6010655682080639 1104 42-43 0.649132976565717 1104 44-45 0.6201320529570111 1104 46-47 0.6126478723464501 1104 48-49 0.7712918734692167 1104 50-51 0.6676014279548355 1104 52-53 0.6401587308963812 1104 54-55 0.7984432650387348 1104 56-57 0.9849094330878998 1104 58-59 0.8367480336730679 1104 60-61 0.9637427313274785 1104 62-63 0.985439210263813 1104 64-65 0.8432376417158913 1104 66-67 0.9720752078174577 1104 68-69 0.7853716171926166 1104 70-71 0.9206612385805926 1104 72-73 0.9992386747354445 1104 74-75 0.7935762331114482 1104 76-77 0.946826450332857 1104 78-79 0.850460297573914 1104 80-81 0.8914760625292395 1104 82-83 0.9803250788087929 1104 84-85 0.9902386298282124 1104 86-87 1.1758534647772052 1104 88-89 1.1501201217419457 1104 90-91 0.9791780508171755 1104 92-93 1.1220110048194911 1104 94-95 1.0165908339980554 1104 96-97 0.8394224085801767 1104 98-99 0.9635198454204534 1104 100-101 0.5731878667156796 1105 1 0.42726171737658447 1105 2 0.4211492623236808 1105 3 0.2792694252258663 1105 4 0.33602545605470624 1105 5 0.3656433994818329 1105 6 0.36429147429744546 1105 7 0.29935983120425647 1105 8 0.21411505480269 1105 9 0.42154854328729385 1105 10-11 0.31044258259118607 1105 12-13 0.27545279899159425 1105 14-15 0.3446928304544983 1105 16-17 0.3682582168261419 1105 18-19 0.3549986879147866 1105 20-21 0.5274622754193885 1105 22-23 0.4777607429988322 1105 24-25 0.523603800517705 1105 26-27 0.6179842883166273 1105 28-29 0.5713019982926468 1105 30-31 0.5503651542964718 1105 32-33 0.48030400248352834 1105 34-35 0.5465315979898548 1105 36-37 0.6142555716095544 1105 38-39 0.6654248720682219 1105 40-41 0.574837664115428 1105 42-43 0.5730783789314629 1105 44-45 0.724655030400946 1105 46-47 0.5843200700305147 1105 48-49 0.7063306980983413 1105 50-51 0.4915430862616432 1105 52-53 0.4315923520968923 1105 54-55 0.5651083938834418 1105 56-57 0.6164880040577216 1105 58-59 0.5599452692955253 1105 60-61 0.6690787024227802 1105 62-63 0.602920768319585 1105 64-65 0.6933649958104731 1105 66-67 0.6754459686523013 1105 68-69 0.6396305473340895 1105 70-71 0.673320852580737 1105 72-73 0.638431427583523 1105 74-75 0.5986160729835177 1105 76-77 0.5636560232941896 1105 78-79 0.6210562676203217 1105 80-81 0.6099660905718203 1105 82-83 0.6502268274105241 1105 84-85 0.4635201418334596 1105 86-87 0.5324297149914017 1105 88-89 0.3849972086341218 1105 90-91 0.30518300811947796 1105 92-93 0.5938518253825897 1105 94-95 0.7712307326532475 1105 96-97 0.5646571414436785 1105 98-99 0.5935814415001559 1105 100-101 0.4656928496795114 1106 1 0.2563599806988499 1106 2 0.23582339729892254 1106 3 0.09521309223196539 1106 4 0.24683809543144264 1106 5 0.25600128266552247 1106 6 -0.09528669598005024 1106 7 0.14937212672718658 1106 8 0.2428412680860319 1106 9 0.23093020263159048 1106 10-11 0.14333127577418736 1106 12-13 0.08598643590908495 1106 14-15 0.3230512371623391 1106 16-17 0.19685133265193144 1106 18-19 -1.4090379529108077E-4 1106 20-21 0.17655590670324273 1106 22-23 0.2406692535066952 1106 24-25 0.20059367975223807 1106 26-27 0.07237729699672713 1106 28-29 0.06670776527867162 1106 30-31 0.13136178884421668 1106 32-33 0.3082904082503717 1106 34-35 0.1939518317492741 1106 36-37 0.09673097951159804 1106 38-39 0.10327061789443093 1106 40-41 0.29766375183434235 1106 42-43 -0.04142478304497388 1106 44-45 0.20611986275047656 1106 46-47 0.08315715063429963 1106 48-49 0.3078662567085715 1106 50-51 0.04291775097542683 1106 52-53 0.2667154917940948 1106 54-55 0.11565386480337736 1106 56-57 -0.266150563259238 1106 58-59 -0.4951544549003941 1106 60-61 0.02335234703601685 1106 62-63 0.14983088867823113 1106 64-65 0.3999625363411603 1106 66-67 -0.14644079161557855 1106 68-69 -0.2103609867810441 1106 70-71 -0.14347880165765758 1106 72-73 0.22307891360850007 1106 74-75 0.4070424971494617 1106 76-77 0.24363828070730875 1106 78-79 0.29300000252183267 1106 80-81 0.19978246573943892 1106 82-83 0.16666614497182408 1106 84-85 0.14260236416260597 1106 86-87 0.24717786157984278 1106 88-89 0.15083840827315242 1106 90-91 0.18607389976723354 1106 92-93 0.03180053656910076 1106 94-95 -0.15562949299239293 1106 96-97 -0.00728864154265807 1106 98-99 -0.1025228907463358 1106 100-101 -0.19031783739543506 1107 1 0.27844277654148897 1107 2 0.3146612515154743 1107 3 0.20890177716600888 1107 4 0.2795821491995767 1107 5 0.3317379481066993 1107 6 0.3135352004113585 1107 7 0.2041032758039094 1107 8 0.26352609892339274 1107 9 0.18165862567654756 1107 10-11 0.2805794253477103 1107 12-13 0.2240904977482856 1107 14-15 0.23098425170322656 1107 16-17 0.3208323301573017 1107 18-19 0.28003346776551297 1107 20-21 0.32732848032268436 1107 22-23 0.42026106683796627 1107 24-25 0.3063124624359901 1107 26-27 0.3067549356727355 1107 28-29 0.3831556426858782 1107 30-31 0.4081274602844829 1107 32-33 0.3268541957962796 1107 34-35 0.46593595774741914 1107 36-37 0.4537794330605891 1107 38-39 0.5741349247761534 1107 40-41 0.4069246829582127 1107 42-43 0.4221578872675096 1107 44-45 0.45173453253653406 1107 46-47 0.33813163299475235 1107 48-49 0.46292289681708354 1107 50-51 0.5765402428125554 1107 52-53 0.5610952492608234 1107 54-55 0.5728401626166146 1107 56-57 0.6157142947537935 1107 58-59 0.5241769847182312 1107 60-61 0.5494213909439978 1107 62-63 0.5585901906404516 1107 64-65 0.4603862972137911 1107 66-67 0.4692701643754127 1107 68-69 0.5586820201652323 1107 70-71 0.5407254770709002 1107 72-73 0.43051268808807563 1107 74-75 0.34381823284560653 1107 76-77 0.5063081235577478 1107 78-79 0.48929094470519274 1107 80-81 0.31876799093127417 1107 82-83 0.31541207889367584 1107 84-85 0.48040681916581107 1107 86-87 0.34574558909353215 1107 88-89 0.657037645863447 1107 90-91 0.8204560162999037 1107 92-93 0.9025745081535526 1107 94-95 0.576299795644104 1107 96-97 0.8932842374174612 1107 98-99 1.0014110291953209 1107 100-101 0.45208237295461373 1108 1 0.1780360359282973 1108 2 0.2817845740300804 1108 3 0.22852106210727285 1108 4 0.2669606482437814 1108 5 0.25231630638885605 1108 6 0.198097876795174 1108 7 0.20736512905988036 1108 8 0.06304720490933846 1108 9 0.15679756460620098 1108 10-11 0.1741237998245353 1108 12-13 0.350113386868955 1108 14-15 0.34374629822965375 1108 16-17 0.30514515526532904 1108 18-19 0.3050980817921669 1108 20-21 0.23670060232132784 1108 22-23 0.25734239480148347 1108 24-25 0.2575305127734353 1108 26-27 0.24847547160216976 1108 28-29 0.17941374539915245 1108 30-31 0.20598097440404928 1108 32-33 0.20205661564789779 1108 34-35 0.44057518059589995 1108 36-37 0.5209671294513143 1108 38-39 0.39861004024121627 1108 40-41 0.4392334551367938 1108 42-43 0.14489088978387343 1108 44-45 0.27613124392171784 1108 46-47 0.32501034830973907 1108 48-49 0.4544325940961542 1108 50-51 0.3628099769300448 1108 52-53 0.5316914192722493 1108 54-55 0.5424202590475389 1108 56-57 0.5326979714167166 1108 58-59 0.52253733149292 1108 60-61 0.4901457418170665 1108 62-63 0.5607041242833972 1108 64-65 0.6318830114807703 1108 66-67 0.5463104597430011 1108 68-69 0.34293895446153044 1108 70-71 0.5305832091811382 1108 72-73 0.4783169206095472 1108 74-75 0.4922695594710511 1108 76-77 0.42155148670988396 1108 78-79 0.2477967726516006 1108 80-81 0.33019536866183685 1108 82-83 0.4712307507542697 1108 84-85 0.43753683477762806 1108 86-87 0.44924032749652554 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-0.7558005859550079 2316 100-101 -0.534866518557374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7281.0 3 2358.0 4 2410.0 5 2658.0 6 3050.0 7 3812.0 8 4935.0 9 6252.0 10 7945.0 11 9909.0 12 12547.0 13 15290.0 14 17903.0 15 21168.0 16 24597.0 17 29003.0 18 27906.0 19 25250.0 20 26875.0 21 29332.0 22 32405.0 23 36520.0 24 41244.0 25 46851.0 26 53867.0 27 61831.0 28 71200.0 29 83796.0 30 98164.0 31 116836.0 32 140556.0 33 170623.0 34 212435.0 35 263417.0 36 313566.0 37 311492.0 38 149758.0 39 8649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.660738997645616 18.43798442917347 14.347077346781292 23.554199226399625 2 15.736900426147876 19.494319499374537 40.46238368393645 24.30639639054114 3 19.666886116149357 23.649713692644752 28.85371545626436 27.829684734941534 4 12.996840059507667 15.976034844402221 36.14729591582907 34.87982918026105 5 14.148049562510256 37.10226791697746 33.70241870217996 15.047263818332318 6 33.19115435420783 35.35272959564459 17.007508334122473 14.448607716025109 7 29.628527198455394 31.94836182664763 19.982734323194695 18.440376651702277 8 26.704027149865816 35.05485733082481 19.19156139808972 19.049554121219657 9 26.686404300557626 16.603161230793916 19.61804469049625 37.09238977815221 10-11 25.390377240634777 26.329497126154344 26.893394307189972 21.38673132602091 12-13 26.318251347004697 23.524432625294203 27.36811469086588 22.78920133683522 14-15 22.95546735727684 24.95368788078631 25.63988546525528 26.450959296681575 16-17 22.60344844530742 28.178716489354557 26.346307473503465 22.871527591834557 18-19 22.638960876409506 26.593001368788098 28.694424073981956 22.07361368082044 20-21 23.017040981968833 25.861999099685374 29.02928911253211 22.091670805813692 22-23 22.738890160465374 25.792492492070814 28.789024331287287 22.679593016176526 24-25 22.555193939455958 25.966677681678984 28.980557600388597 22.497570778476465 26-27 22.21523210294306 26.367102162688518 28.758384966821232 22.659280767547195 28-29 22.27336908041368 26.66267352080926 28.714643658871676 22.349313739905387 30-31 22.43026632833058 26.274221947698067 28.654203278896368 22.641308445074984 32-33 22.099397529334663 26.29649147548041 28.726715270072678 22.87739572511225 34-35 22.7666728719691 26.283354169241065 28.581576189612644 22.368396769177192 36-37 22.77421507046257 26.0295771684143 28.40532311199971 22.79088464912342 38-39 22.3661774550863 26.33704216386375 28.57840508387487 22.71837529717508 40-41 22.760330230445337 26.174081943919386 28.51148616736342 22.554101658271854 42-43 22.47479619360855 26.203375214335132 28.419445085082273 22.90238350697404 44-45 22.460326674442623 26.511043243588993 28.36656562733913 22.662064454629252 46-47 22.703822632514363 26.680582072788578 27.844775777006454 22.770819517690608 48-49 22.672714344096775 26.62513450460795 27.82874771046387 22.873403440831407 50-51 22.75965276495261 26.8590411873897 27.68856807753213 22.692737970125563 52-53 22.858801483482274 26.7631391351166 27.666262609248406 22.711796772152724 54-55 22.53297016537671 26.84472404161579 27.76993605391131 22.852369739096186 56-57 22.575609462650203 27.03655021330566 27.65572315882875 22.732117165215385 58-59 22.596660177603027 26.641155582720323 27.707098586442907 23.055085653233743 60-61 22.46143924717523 26.83333898149762 27.901503768595575 22.803718002731575 62-63 22.47074893005409 26.860534876707582 27.95967571174663 22.709040481491698 64-65 22.295233704809608 26.75681902627992 27.8647510075618 23.083196261348668 66-67 22.293946007248973 26.878653862657675 27.758227682613928 23.06917244747942 68-69 22.54843140596958 26.63052567200393 27.92648813402982 22.89455478799667 70-71 22.407929432017912 26.733575544433492 27.67299255317186 23.185502470376736 72-73 22.487648892636866 26.734985896624714 27.563841211937685 23.213523998800735 74-75 22.346412633168946 26.99276375939575 27.535015539176435 23.12580806825887 76-77 22.53081486974505 26.73902707489397 27.54168657757675 23.188471477784237 78-79 22.58570204564426 26.782161213667248 27.53179030918165 23.10034643150684 80-81 22.549214623361298 26.97180328026639 27.340599642994214 23.1383824533781 82-83 22.609709924619263 26.969914076023997 27.32214402687904 23.0982319724777 84-85 22.56095898156934 26.88802203434284 27.32845624051056 23.22256274357726 86-87 22.64650998456386 26.94627108332508 27.22465499284339 23.182563939267673 88-89 22.651014989685038 26.87545552894306 27.138734711576223 23.33479476979568 90-91 22.598959806322597 26.911611618237284 27.155593798953387 23.33383477648673 92-93 22.715390142849724 26.567272775404717 27.293504247347077 23.423832834398485 94-95 22.583630372885924 26.506558135915352 27.344767250155837 23.565044241042884 96-97 22.778198762772124 26.34925913549695 27.243964793441673 23.628577308289252 98-99 22.649440494339967 26.412962867146135 27.015064678364965 23.922531960148934 100-101 23.132446435848486 25.86321944991478 27.202739786526305 23.801594327710426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1169.0 1 814.0 2 709.0 3 932.0 4 745.0 5 653.0 6 1149.0 7 2471.0 8 3897.5 9 4273.5 10 3462.0 11 2611.0 12 2468.5 13 2573.5 14 2711.0 15 2825.0 16 3008.5 17 3226.0 18 3391.5 19 3686.0 20 4129.5 21 4747.0 22 5595.0 23 6870.0 24 8337.0 25 10182.5 26 12824.5 27 15839.5 28 18837.5 29 22169.5 30 25824.5 31 30309.0 32 36586.5 33 40810.5 34 43271.5 35 46752.5 36 51377.0 37 56624.5 38 60412.5 39 63561.5 40 67370.0 41 72119.0 42 76001.5 43 79384.5 44 83793.0 45 87520.0 46 88683.0 47 90769.0 48 93492.0 49 93852.0 50 92799.0 51 90461.0 52 88586.5 53 88446.5 54 88620.0 55 84717.0 56 78127.5 57 72342.5 58 66953.0 59 59389.0 60 49623.0 61 41318.5 62 34389.0 63 26932.0 64 20296.0 65 15778.5 66 12120.0 67 9364.5 68 7180.0 69 5420.5 70 4287.0 71 3191.0 72 2333.5 73 1859.0 74 1440.0 75 1109.5 76 821.0 77 548.5 78 359.5 79 231.0 80 133.5 81 83.5 82 65.5 83 38.5 84 22.0 85 19.0 86 13.0 87 7.5 88 6.0 89 6.0 90 4.0 91 2.5 92 2.0 93 1.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1721143477680274 2 0.045274254107666105 3 0.04371030733158198 4 0.049685386040211076 5 0.05630208393902854 6 0.06123453146360154 7 0.03544946025790685 8 0.03452713267201109 9 0.039740288592291506 10-11 0.04162504496346981 12-13 0.037614925024792564 14-15 0.03300328709531373 16-17 0.030897974127508183 18-19 0.039098669402103145 20-21 0.04928437404634335 22-23 0.04649734068896267 24-25 0.0656657139958399 26-27 0.051489940012615844 28-29 0.04356995313372828 30-31 0.05203130620433726 32-33 0.048301894661367424 34-35 0.04808133806474018 36-37 0.045174001109199176 38-39 0.025343958012440198 40-41 0.02339904984218173 42-43 0.020692218883574587 44-45 0.0043108789340780395 46-47 0.011689499621244171 48-49 0.011308538227069833 50-51 0.0088824156641701 52-53 0.00936363005681137 54-55 0.010526564839027771 56-57 0.008160594075208196 58-59 0.010626817837494702 60-61 0.010245856443320362 62-63 0.004290828334384653 64-65 0.006877355694831476 66-67 0.0038898163405169284 68-69 0.00866185906754285 70-71 0.02201555846333808 72-73 0.01978994189737221 74-75 0.019108221507797077 76-77 0.011729600820630945 78-79 0.0049925993236531714 80-81 0.006977608693298408 82-83 0.005995129308322482 84-85 0.005173054720893647 86-87 0.0076994302822603125 88-89 0.028471851564608446 90-91 0.0355296626566804 92-93 0.021915305464871148 94-95 0.03035660793578675 96-97 0.034487031472624316 98-99 0.02037140928848041 100-101 0.11974218136890256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2493691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.96088718286275 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9737996914037 44.41456807441185 2 12.521027152450523 14.264176300885909 3 3.92408436324408 6.705579801323455 4 1.7986798721191128 4.098176050954511 5 0.9669481028714307 2.753911089967136 6 0.6096063313843236 2.0834230480764777 7 0.40686329629576734 1.6222706023405604 8 0.27305869933414706 1.2442932613657274 9 0.21164486999181967 1.0849931606192127 >10 1.1986629931256538 12.459279225353766 >50 0.07809656900090367 3.047548460693205 >100 0.03364105546312277 3.3510157220577472 >500 0.002614893139488017 1.060016957606965 >1k 0.0010600918133039498 0.8835755811363891 >5k 2.1201836266078995E-4 0.927172663207131 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9980 0.40020996987998914 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7525 0.30176152538546275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5527 0.2216393290106914 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.010119938677246E-5 2 4.010119938677246E-5 0.0 0.0 4.010119938677246E-5 4.010119938677246E-5 3 4.010119938677246E-5 0.0 0.0 4.010119938677246E-5 4.010119938677246E-5 4 4.010119938677246E-5 0.0 0.0 4.010119938677246E-5 4.010119938677246E-5 5 8.020239877354492E-5 0.0 0.0 8.020239877354492E-5 4.010119938677246E-5 6 8.020239877354492E-5 0.0 0.0 8.020239877354492E-5 4.010119938677246E-5 7 8.020239877354492E-5 0.0 0.0 8.020239877354492E-5 4.010119938677246E-5 8 8.020239877354492E-5 0.0 0.0 8.020239877354492E-5 4.010119938677246E-5 9 8.020239877354492E-5 0.0 0.0 1.2030359816031738E-4 4.010119938677246E-5 10-11 8.020239877354492E-5 0.0 0.0 1.2030359816031738E-4 4.010119938677246E-5 12-13 8.020239877354492E-5 0.0 0.0 1.2030359816031738E-4 4.010119938677246E-5 14-15 8.020239877354492E-5 0.0 0.0 1.2030359816031738E-4 4.010119938677246E-5 16-17 1.0025299846693115E-4 0.0 0.0 1.2030359816031738E-4 4.010119938677246E-5 18-19 1.2030359816031738E-4 0.0 0.0 1.6040479754708984E-4 4.010119938677246E-5 20-21 1.2030359816031738E-4 4.010119938677246E-5 0.0 2.005059969338623E-4 4.010119938677246E-5 22-23 1.2030359816031738E-4 4.010119938677246E-5 0.0 4.4111319325449707E-4 4.010119938677246E-5 24-25 1.2030359816031738E-4 4.010119938677246E-5 0.0 7.218215889619043E-4 4.010119938677246E-5 26-27 1.2030359816031738E-4 4.010119938677246E-5 0.0 9.624287852825391E-4 4.010119938677246E-5 28-29 1.403541978537036E-4 4.010119938677246E-5 0.0 0.0023258695644328025 4.010119938677246E-5 30-31 1.6040479754708984E-4 4.010119938677246E-5 0.0 0.007879885679500789 4.010119938677246E-5 32-33 1.6040479754708984E-4 4.010119938677246E-5 0.0 0.020291206889706863 4.010119938677246E-5 34-35 1.6040479754708984E-4 4.010119938677246E-5 0.0 0.0384369996122214 4.010119938677246E-5 36-37 1.6040479754708984E-4 4.010119938677246E-5 0.0 0.07152048910630868 4.010119938677246E-5 38-39 1.6040479754708984E-4 4.010119938677246E-5 0.0 0.1226094171250568 4.010119938677246E-5 40-41 1.6040479754708984E-4 4.010119938677246E-5 0.0 0.17698664349352025 4.010119938677246E-5 42-43 1.8045539724047607E-4 4.010119938677246E-5 0.0 0.23565469819636836 4.010119938677246E-5 44-45 2.005059969338623E-4 4.010119938677246E-5 0.0 0.30661377051126226 4.010119938677246E-5 46-47 2.2055659662724854E-4 4.010119938677246E-5 0.0 0.39423489117136 4.010119938677246E-5 48-49 2.4060719632063477E-4 4.010119938677246E-5 0.0 0.48971584691126524 4.010119938677246E-5 50-51 2.4060719632063477E-4 4.010119938677246E-5 0.0 0.5949012929027695 4.010119938677246E-5 52-53 2.4060719632063477E-4 4.010119938677246E-5 0.0 0.6977608693298407 4.010119938677246E-5 54-55 2.4060719632063477E-4 4.010119938677246E-5 0.0 0.8151370799349238 4.010119938677246E-5 56-57 2.4060719632063477E-4 4.010119938677246E-5 0.0 0.9502981724680404 4.010119938677246E-5 58-59 2.4060719632063477E-4 4.010119938677246E-5 0.0 1.102021060347894 4.010119938677246E-5 60-61 2.4060719632063477E-4 4.010119938677246E-5 0.0 1.2632880336817993 4.010119938677246E-5 62-63 2.4060719632063477E-4 4.010119938677246E-5 0.0 1.4278032041660333 4.010119938677246E-5 64-65 2.4060719632063477E-4 4.010119938677246E-5 0.0 1.5996368435383534 4.010119938677246E-5 66-67 2.4060719632063477E-4 4.010119938677246E-5 0.0 1.795731708539671 4.010119938677246E-5 68-69 2.4060719632063477E-4 4.010119938677246E-5 0.0 2.02031847570529 4.010119938677246E-5 70-71 2.807083957074072E-4 4.010119938677246E-5 0.0 2.26054471063175 4.010119938677246E-5 72-73 3.208095950941797E-4 4.010119938677246E-5 0.0 2.5039589909094593 4.010119938677246E-5 74-75 3.208095950941797E-4 4.010119938677246E-5 0.0 2.7571780144372338 4.010119938677246E-5 76-77 3.408601947875659E-4 4.010119938677246E-5 0.0 3.0344778081967654 4.010119938677246E-5 78-79 3.809613941743384E-4 4.010119938677246E-5 0.0 3.3407106173138534 4.010119938677246E-5 80-81 4.010119938677246E-4 4.010119938677246E-5 0.0 3.672748548236329 4.010119938677246E-5 82-83 4.010119938677246E-4 4.010119938677246E-5 0.0 4.022651563485613 4.010119938677246E-5 84-85 4.010119938677246E-4 4.010119938677246E-5 0.0 4.3693063815845665 4.010119938677246E-5 86-87 4.010119938677246E-4 4.010119938677246E-5 0.0 4.726287258525615 4.010119938677246E-5 88-89 4.010119938677246E-4 4.010119938677246E-5 0.0 5.117574711542048 4.010119938677246E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4050 0.0 56.528454 1 GTATCAA 7490 0.0 45.372574 1 ATCAACG 9855 0.0 34.241333 3 TCAACGC 10055 0.0 33.654785 4 TATCAAC 10095 0.0 33.33311 2 CAACGCA 10325 0.0 32.634647 5 AACGCAG 10650 0.0 31.8574 6 ACGCAGA 12400 0.0 27.397518 7 GTGGTAT 1925 0.0 26.95087 1 GTACATG 9890 0.0 26.854366 1 CGCAGAG 12550 0.0 26.842897 8 TACATGG 9655 0.0 26.582167 2 TGGTATC 1825 0.0 24.740566 2 ACATGGG 10060 0.0 24.236408 3 GCAGAGT 13645 0.0 24.095352 9 CATGGGG 5155 0.0 21.666468 4 TAAGGTG 1045 0.0 21.374912 5 GAGTACT 8175 0.0 21.237768 12-13 AGAGTAC 12675 0.0 21.08212 10-11 CAGAGTA 13105 0.0 20.607874 10-11 >>END_MODULE