FastQCFastQC Report
Thu 26 May 2016
SRR938334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493691
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101050.4052226198033357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81200.3256217390205924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63240.253599984921949No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.056.4926721
GTATCAA81850.043.6972661
ATCAACG107300.033.183943
TATCAAC109900.032.4577672
TCAACGC110050.032.354074
CAACGCA114250.031.1230775
AACGCAG116600.030.5503776
GTGGTAT23050.028.887651
TGGTATC21500.028.7580852
ACGCAGA133850.026.5067257
CGCAGAG135650.026.0849698
TACATGG92300.025.0947232
GTACATG94350.024.8519291
GCAGAGT149950.023.1539339
ACATGGG96150.022.990563
TATACCG1503.016569E-522.1842085
GAGTACT94150.021.0363112-13
AGAGTAC140950.020.3528710-11
CAGAGTA144600.020.21685410-11
AGTACTT97900.020.15775712-13
Produced by FastQC (version 0.11.5)