FastQCFastQC Report
Thu 26 May 2016
SRR938333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507785
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103070.41100014554676734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75880.30257777281545273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56450.22509904158450586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.056.255641
GTATCAA84300.043.8018231
ATCAACG107500.034.173983
TATCAAC109450.033.7822342
TCAACGC109800.033.2854
CAACGCA113050.032.370155
AACGCAG116400.031.597446
TGGTATC19400.029.1520962
GTGGTAT21600.027.3518051
ACGCAGA134800.027.1006077
CGCAGAG137700.026.4953638
GTACATG100400.025.9580271
TACATGG100300.025.3025862
GCAGAGT148000.024.201579
ACATGGG101850.023.6576443
GAGTACT89150.022.29927412-13
CAGAGTA142400.021.08257710-11
GTACTTT94500.020.93838114-15
AGAGTAC137100.020.35574510-11
AGTACTT92000.020.13693612-13
Produced by FastQC (version 0.11.5)