FastQCFastQC Report
Thu 26 May 2016
SRR938333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507785
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101750.4057365364255707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83780.3340796758892808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63410.2528526169508152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45050.056.586061
GTATCAA80850.043.0008241
ATCAACG109250.031.5014953
TCAACGC111600.030.8807534
TATCAAC111700.030.7839642
CAACGCA114750.029.991625
AACGCAG118500.029.0983586
GTGGTAT20450.026.977651
TGGTATC20200.025.8988672
ACGCAGA136450.025.061627
CGCAGAG138450.024.6995898
GTACATG98600.022.4292891
GCAGAGT151250.022.2324899
TACATGG97600.022.0743452
AGTACTT95400.020.78539712-13
ACATGGG99650.020.750383
GAGTACT91050.020.7090812-13
AGAGTAC141950.020.56064410-11
GTAAGGT10700.019.991384
CAGAGTA146500.019.6789210-11
Produced by FastQC (version 0.11.5)