##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938333_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2507785 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.594127885763733 31.0 31.0 34.0 30.0 34.0 2 31.849457190309376 31.0 31.0 34.0 30.0 34.0 3 31.909510982799564 31.0 31.0 34.0 30.0 34.0 4 35.621272158498435 37.0 35.0 37.0 33.0 37.0 5 35.488458938864376 37.0 35.0 37.0 33.0 37.0 6 35.535405547126246 37.0 35.0 37.0 33.0 37.0 7 35.46213650691746 37.0 35.0 37.0 33.0 37.0 8 35.44198166908248 37.0 35.0 37.0 33.0 37.0 9 36.97452772067781 39.0 37.0 39.0 33.0 39.0 10-11 36.89072308830302 39.0 37.0 39.0 33.0 39.0 12-13 36.76503647641245 39.0 37.0 39.0 32.0 39.0 14-15 37.72697539860873 39.5 37.0 41.0 32.0 41.0 16-17 37.62231411384947 39.0 36.0 41.0 32.0 41.0 18-19 37.61781193363865 39.0 36.5 41.0 32.0 41.0 20-21 37.606989434899724 39.0 36.5 41.0 32.0 41.0 22-23 37.635686472325176 39.0 37.0 41.0 32.0 41.0 24-25 37.54225023277514 39.0 36.0 41.0 32.0 41.0 26-27 37.334468265820234 39.0 36.0 41.0 31.5 41.0 28-29 37.175108910851606 39.0 36.0 40.5 31.0 41.0 30-31 36.88690577541536 39.0 36.0 40.0 30.5 41.0 32-33 36.63282697679426 38.5 35.0 40.0 30.0 41.0 34-35 36.42761779817648 38.0 35.0 40.0 30.0 41.0 36-37 36.40532760982301 38.0 35.0 40.0 30.0 41.0 38-39 36.22118303602582 38.0 35.0 40.0 30.0 41.0 40-41 36.0059989193651 38.0 35.0 40.0 29.5 41.0 42-43 35.74051483679821 38.0 35.0 40.0 28.5 41.0 44-45 35.40724962466878 38.0 34.0 40.0 27.0 41.0 46-47 34.97341498573442 38.0 33.5 40.0 26.0 41.0 48-49 34.51180862793262 38.0 33.0 40.0 24.5 41.0 50-51 34.08325554224146 37.5 33.0 40.0 24.0 41.0 52-53 33.48192668031749 37.0 32.0 40.0 22.5 41.0 54-55 33.08307968984582 37.0 31.5 39.0 21.5 40.5 56-57 32.65157738801372 36.0 31.0 39.0 19.0 40.0 58-59 32.10625831161762 35.5 30.5 39.0 17.5 40.0 60-61 31.619913788462725 35.0 30.0 39.0 13.5 40.0 62-63 31.05152694509298 35.0 29.0 38.0 10.0 40.0 64-65 30.46315812559689 34.0 28.0 38.0 8.0 40.0 66-67 29.879618866848634 34.0 28.0 37.5 6.5 39.5 68-69 29.225641751585563 34.0 26.5 37.0 2.0 39.0 70-71 28.574486249818065 33.0 26.0 36.0 2.0 39.0 72-73 27.908754538367525 32.5 26.0 35.5 2.0 38.5 74-75 27.21618579742681 32.0 24.5 35.0 2.0 37.5 76-77 26.057093211738646 30.5 23.0 34.0 2.0 36.0 78-79 26.619613722866994 32.0 24.0 35.0 2.0 36.0 80-81 26.230802481073937 32.0 23.0 35.0 2.0 36.0 82-83 25.64177949864123 31.0 21.0 34.5 2.0 36.0 84-85 24.947044304037227 31.0 19.0 34.0 2.0 35.0 86-87 24.178823942243852 31.0 14.0 34.0 2.0 35.0 88-89 23.312472361067634 30.0 7.0 34.0 2.0 35.0 90-91 22.64459194069667 29.5 2.0 34.0 2.0 35.0 92-93 21.7335822249515 29.0 2.0 34.0 2.0 35.0 94-95 20.813452508887323 29.0 2.0 34.0 2.0 35.0 96-97 19.71873884722973 27.0 2.0 33.0 2.0 35.0 98-99 18.46379813261504 25.0 2.0 33.0 2.0 35.0 100-101 15.655490402885414 11.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3687292794163284 1101 2 0.38844604929776594 1101 3 0.34342015910590007 1101 4 0.26770739408902955 1101 5 0.2632243679478634 1101 6 0.310229246497272 1101 7 0.35482768927230524 1101 8 0.40607030520444454 1101 9 0.4246135475348325 1101 10-11 0.48516334506122405 1101 12-13 0.5248762351720586 1101 14-15 0.6387326491669114 1101 16-17 0.6222710799585229 1101 18-19 0.7709314869293351 1101 20-21 0.6621699549179922 1101 22-23 0.6779977977882581 1101 24-25 0.7004854351432357 1101 26-27 0.751486864355492 1101 28-29 0.7609052022225029 1101 30-31 0.7843278171244137 1101 32-33 0.7911554123670257 1101 34-35 0.8141368527690815 1101 36-37 0.9664942597686874 1101 38-39 1.039530083474503 1101 40-41 1.246110159408822 1101 42-43 1.2440046466498558 1101 44-45 1.2934455183758757 1101 46-47 1.3256994532122164 1101 48-49 1.3643770947627587 1101 50-51 1.4682318300094934 1101 52-53 1.5836837654710223 1101 54-55 1.4634839299167979 1101 56-57 1.6661386508722273 1101 58-59 1.9044574601290805 1101 60-61 1.917508423884744 1101 62-63 2.0031727142106597 1101 64-65 2.113407212143013 1101 66-67 2.284902008846519 1101 68-69 2.36417086940725 1101 70-71 2.470670676593844 1101 72-73 2.6448617972535367 1101 74-75 2.762837463212584 1101 76-77 2.6744429203505184 1101 78-79 3.1557913409908913 1101 80-81 3.413452401984717 1101 82-83 3.5257719433370127 1101 84-85 3.5642017898154457 1101 86-87 3.58533086247375 1101 88-89 3.8209909372008184 1101 90-91 4.091870918391908 1101 92-93 4.520639170693304 1101 94-95 4.652795623224005 1101 96-97 4.866945756624034 1101 98-99 5.010138546159645 1101 100-101 5.161589687045646 1102 1 0.3649090050860764 1102 2 0.39406447620222806 1102 3 0.36309425195072365 1102 4 0.2274281365148667 1102 5 0.12332041873127508 1102 6 0.15763264378980324 1102 7 0.2704041059573683 1102 8 0.3196821922087949 1102 9 0.4407841224637181 1102 10-11 0.41696472919077365 1102 12-13 0.36229060695450244 1102 14-15 0.6791526873696512 1102 16-17 0.5012861274879867 1102 18-19 0.5985952004023574 1102 20-21 0.6157955360380285 1102 22-23 0.5533861443517694 1102 24-25 0.572024711338841 1102 26-27 0.5913952929557098 1102 28-29 0.41558471832108523 1102 30-31 0.4956747357884055 1102 32-33 0.5810019294862698 1102 34-35 0.5272883231267613 1102 36-37 0.8111860301810765 1102 38-39 0.9593651141742825 1102 40-41 0.8045549174101225 1102 42-43 0.8019214772939591 1102 44-45 0.7723812168237671 1102 46-47 0.8781434369200554 1102 48-49 1.0844588115185374 1102 50-51 1.145609022907749 1102 52-53 1.1737481726085974 1102 54-55 1.2092549118296887 1102 56-57 1.4233720555557454 1102 58-59 1.398520677030433 1102 60-61 1.7818006972679221 1102 62-63 1.9286293713746296 1102 64-65 1.9922622816854378 1102 66-67 2.0043294156353433 1102 68-69 2.0287578222425395 1102 70-71 2.339010393048863 1102 72-73 2.2470061421916725 1102 74-75 2.314574344217757 1102 76-77 2.3233184350796883 1102 78-79 2.7362058759508763 1102 80-81 3.0165470161658057 1102 82-83 3.214557166490305 1102 84-85 3.593636139648911 1102 86-87 3.7339696097821182 1102 88-89 3.8278763897625474 1102 90-91 3.881435874253981 1102 92-93 4.003731097244209 1102 94-95 4.294257758034256 1102 96-97 4.667992332615178 1102 98-99 4.982243184561401 1102 100-101 5.100366111409654 1103 1 0.3121090319770019 1103 2 0.34914631512854655 1103 3 0.38393574912644013 1103 4 0.2395411409002719 1103 5 0.23980824012129176 1103 6 0.2831343791683807 1103 7 0.30496351204538996 1103 8 0.35233166460712084 1103 9 0.3485094835819851 1103 10-11 0.44244631112007227 1103 12-13 0.5687825239028328 1103 14-15 0.701932350755456 1103 16-17 0.6654033901925445 1103 18-19 0.5519056882455757 1103 20-21 0.6412914185129068 1103 22-23 0.6018217236373928 1103 24-25 0.5737337739631343 1103 26-27 0.5414316930041849 1103 28-29 0.5328620182837298 1103 30-31 0.7375544584698659 1103 32-33 0.815506698644505 1103 34-35 0.9020804123598012 1103 36-37 0.9100785782404941 1103 38-39 1.12869684230823 1103 40-41 1.0192336755566416 1103 42-43 0.9398341696620989 1103 44-45 1.031647085785366 1103 46-47 1.1860097100121152 1103 48-49 1.286928515739028 1103 50-51 1.326698895497259 1103 52-53 1.4306997857543635 1103 54-55 1.6469083662665227 1103 56-57 1.7255843057274234 1103 58-59 1.800832986792642 1103 60-61 1.8822673373385221 1103 62-63 1.9797225669981309 1103 64-65 2.058054374161909 1103 66-67 2.1584547986909364 1103 68-69 2.196120497936537 1103 70-71 2.3321821773942766 1103 72-73 2.1510163837411582 1103 74-75 2.384823562921458 1103 76-77 2.114042924306954 1103 78-79 2.4856376872638783 1103 80-81 2.831183860445247 1103 82-83 3.0793319446917913 1103 84-85 3.223795946034265 1103 86-87 3.355190690614407 1103 88-89 3.561459289730376 1103 90-91 3.9965506221265166 1103 92-93 4.302221225002629 1103 94-95 4.227186503870513 1103 96-97 4.51723118976275 1103 98-99 4.978322402507317 1103 100-101 4.964694697846431 1104 1 0.31484120033779917 1104 2 0.3294036188993239 1104 3 0.34382645671890444 1104 4 0.22450485428922917 1104 5 0.23396522350334692 1104 6 0.29103432257913653 1104 7 0.23870176339523397 1104 8 0.24574192527220617 1104 9 0.34578653402015647 1104 10-11 0.43210303141177775 1104 12-13 0.3734791474113379 1104 14-15 0.512460715581625 1104 16-17 0.5851814724270668 1104 18-19 0.5930399692714374 1104 20-21 0.6072434864313436 1104 22-23 0.6137825479965784 1104 24-25 0.5789085694830831 1104 26-27 0.5005213578795704 1104 28-29 0.5881407734905792 1104 30-31 0.6575557065534241 1104 32-33 0.703561120760547 1104 34-35 0.7629629208942887 1104 36-37 0.8918803854087471 1104 38-39 0.7577483980869246 1104 40-41 0.8663074160204971 1104 42-43 0.9422158818341728 1104 44-45 0.9710290149537855 1104 46-47 1.0088921873445074 1104 48-49 1.1417011588821495 1104 50-51 1.3228190575779237 1104 52-53 1.6057520137962555 1104 54-55 1.625060663662147 1104 56-57 1.7821755342529428 1104 58-59 1.9033117800093038 1104 60-61 1.9474209863518972 1104 62-63 2.097607887975439 1104 64-65 2.123967652210812 1104 66-67 2.1161200882108666 1104 68-69 2.2626318632973934 1104 70-71 2.3734701287076696 1104 72-73 2.576047337631561 1104 74-75 2.757824040880724 1104 76-77 2.806105112906561 1104 78-79 3.126451508654611 1104 80-81 3.3518690724988964 1104 82-83 3.5376194322286842 1104 84-85 3.694273309627949 1104 86-87 4.012860164050792 1104 88-89 4.102945870704762 1104 90-91 4.202363642190878 1104 92-93 4.597938170998905 1104 94-95 4.750455674362982 1104 96-97 4.900570621686175 1104 98-99 5.14007793130472 1104 100-101 5.09485002751777 1105 1 0.29145241337730354 1105 2 0.29982010518709146 1105 3 0.24084831520564265 1105 4 0.20200731087804513 1105 5 0.10372822280931615 1105 6 0.1797992940277311 1105 7 0.18727213178048174 1105 8 0.19005603880440702 1105 9 0.3208083512774351 1105 10-11 0.33664342597774066 1105 12-13 0.3750166627864928 1105 14-15 0.5511947649332853 1105 16-17 0.5387866453503065 1105 18-19 0.5314661719692211 1105 20-21 0.5437576728022577 1105 22-23 0.4398862950675664 1105 24-25 0.34329799550025086 1105 26-27 0.5676721271526262 1105 28-29 0.5884819472933742 1105 30-31 0.6293430724717766 1105 32-33 0.6145109788624765 1105 34-35 0.7150889868476824 1105 36-37 0.8807120111551683 1105 38-39 0.8646295953905181 1105 40-41 0.8952422528549064 1105 42-43 0.931807847586235 1105 44-45 0.9224857892980651 1105 46-47 1.038651613430396 1105 48-49 1.2256546520483695 1105 50-51 1.2494735615531383 1105 52-53 1.4082360889163326 1105 54-55 1.3235671263804036 1105 56-57 1.487899557973627 1105 58-59 1.63571676327701 1105 60-61 1.7787227379168975 1105 62-63 1.865286737948292 1105 64-65 1.9057720596403662 1105 66-67 2.121253156301325 1105 68-69 2.125040213637881 1105 70-71 2.2779297822427544 1105 72-73 2.508455756687816 1105 74-75 2.5990397603004887 1105 76-77 2.4139191268008915 1105 78-79 2.8054584970485195 1105 80-81 3.092806493046286 1105 82-83 3.157474167088882 1105 84-85 3.330125075966844 1105 86-87 3.5205779758657805 1105 88-89 3.6809688492585693 1105 90-91 3.9419009789050286 1105 92-93 4.382479527585655 1105 94-95 4.301926191861451 1105 96-97 4.614863158466292 1105 98-99 4.9938445862897005 1105 100-101 4.999107313107386 1106 1 0.1723440742301321 1106 2 0.15989317744400822 1106 3 0.1795836159982862 1106 4 0.09154183948073324 1106 5 0.07284230574968831 1106 6 0.12387856397760544 1106 7 0.12041914206607629 1106 8 0.037501171570085035 1106 9 0.10534970238570907 1106 10-11 0.30765310076697006 1106 12-13 0.11068491810230086 1106 14-15 0.16328937221193485 1106 16-17 0.15642261214277653 1106 18-19 0.19285947610727305 1106 20-21 0.44600391622141444 1106 22-23 0.34090784970224774 1106 24-25 0.27067375445145814 1106 26-27 0.24819170228003884 1106 28-29 0.39106175099574614 1106 30-31 0.5943175616244147 1106 32-33 0.533843886314564 1106 34-35 0.5111126008423668 1106 36-37 0.5157540529195543 1106 38-39 0.40738316985109435 1106 40-41 0.49083158004658145 1106 42-43 0.7389356147636761 1106 44-45 0.25422809191471174 1106 46-47 -0.011366738689353895 1106 48-49 0.5859196510537075 1106 50-51 0.35751277064682085 1106 52-53 0.6329224918168066 1106 54-55 0.6118628168543907 1106 56-57 0.5362967594855093 1106 58-59 0.989231404345972 1106 60-61 0.9217381452919291 1106 62-63 1.0855593860050696 1106 64-65 0.6468045975283836 1106 66-67 0.9561437580547221 1106 68-69 0.8289574806009803 1106 70-71 1.0734493433609167 1106 72-73 1.4895983232387664 1106 74-75 1.7939071907515611 1106 76-77 1.7495299441846903 1106 78-79 2.22174299132854 1106 80-81 2.340358656495031 1106 82-83 2.639371552121961 1106 84-85 2.593581242419166 1106 86-87 3.0065248751980995 1106 88-89 3.1986170041000257 1106 90-91 3.0953104104936493 1106 92-93 3.1258734649418862 1106 94-95 3.427589459883656 1106 96-97 3.954148882758613 1106 98-99 4.20410846455491 1106 100-101 4.351161349593083 1107 1 0.24790122101981638 1107 2 0.20307647181428123 1107 3 0.1856983628298856 1107 4 0.1553950988632593 1107 5 0.05296657170978136 1107 6 0.11901337948731339 1107 7 0.10634378909070819 1107 8 0.22839146706558466 1107 9 0.1451248726463774 1107 10-11 0.2668520834859933 1107 12-13 0.3575244194084206 1107 14-15 0.5124802961894801 1107 16-17 0.4699143093104112 1107 18-19 0.43695804890332823 1107 20-21 0.391142515763498 1107 22-23 0.3180764121275601 1107 24-25 0.3387077458737622 1107 26-27 0.43632776277798513 1107 28-29 0.39349933317370045 1107 30-31 0.3525226744758996 1107 32-33 0.4754107335336286 1107 34-35 0.5632276670296648 1107 36-37 0.6640471632361553 1107 38-39 0.6515270678701697 1107 40-41 0.6970971060146027 1107 42-43 0.8178988065242976 1107 44-45 0.7793632151369394 1107 46-47 0.6799618392380182 1107 48-49 0.9270717932734485 1107 50-51 0.9130216148868939 1107 52-53 0.9364715399455363 1107 54-55 1.0151071880022684 1107 56-57 1.0483333090800357 1107 58-59 1.1883960480107163 1107 60-61 1.1710894540265606 1107 62-63 1.4121332316049084 1107 64-65 1.5877319016922335 1107 66-67 1.7468418579714715 1107 68-69 1.8217532651057446 1107 70-71 1.6819787186795452 1107 72-73 1.716195538357269 1107 74-75 1.87179276493897 1107 76-77 2.0717203417243226 1107 78-79 2.297446509480668 1107 80-81 2.4906537694380013 1107 82-83 2.631851515462589 1107 84-85 2.8640220863498733 1107 86-87 3.126316528870152 1107 88-89 3.41870173315915 1107 90-91 3.56236209205942 1107 92-93 3.7823234520928075 1107 94-95 3.9643322063925694 1107 96-97 4.321382156295922 1107 98-99 4.445345726711842 1107 100-101 4.393419011426998 1108 1 0.20646786566585718 1108 2 0.16704343185689297 1108 3 0.19347064524773927 1108 4 0.20830952365681554 1108 5 0.13102537784750723 1108 6 0.04560200484405641 1108 7 0.11804643166126283 1108 8 0.09953035705937907 1108 9 0.22272659022770114 1108 10-11 0.3060489307778269 1108 12-13 0.3668773752910397 1108 14-15 0.3140695537335887 1108 16-17 0.32072950296682734 1108 18-19 0.40911050269931337 1108 20-21 0.4695894507755938 1108 22-23 0.28047103674425955 1108 24-25 0.3675280404963175 1108 26-27 0.4638279818223694 1108 28-29 0.4151352454624515 1108 30-31 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1.1723483598328635 2314 68-69 1.3815093684354345 2314 70-71 1.4967473767791226 2314 72-73 1.6216602067535177 2314 74-75 1.5759024900585281 2314 76-77 1.4164108071947261 2314 78-79 1.6102593842800026 2314 80-81 1.8122573483322242 2314 82-83 2.023732133555473 2314 84-85 2.2046487540658184 2314 86-87 2.4483873369982625 2314 88-89 2.454244912407894 2314 90-91 2.6180892301868326 2314 92-93 2.9005722261804863 2314 94-95 3.143899818311496 2314 96-97 3.168538852467048 2314 98-99 3.4447084200163793 2314 100-101 3.433638568313933 2315 1 -0.3378203793506245 2315 2 -0.407774470758671 2315 3 -0.3579471727492347 2315 4 -0.2382125913209805 2315 5 -0.2117744322172328 2315 6 -0.323590739872607 2315 7 -0.30342477518858146 2315 8 -0.32776263938237804 2315 9 -0.4541248980550563 2315 10-11 -0.4232791732482539 2315 12-13 -0.5311956493366239 2315 14-15 -0.5652723997383617 2315 16-17 -0.5048307908240588 2315 18-19 -0.5155840485329861 2315 20-21 -0.41636667844125697 2315 22-23 -0.5161647654430368 2315 24-25 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90-91 3.021747527370511 2315 92-93 3.190229355083929 2315 94-95 3.6074207468070014 2315 96-97 3.5269547793825033 2315 98-99 3.8254971528153696 2315 100-101 3.7288601106353614 2316 1 -0.5406916623442761 2316 2 -0.8177574866847195 2316 3 -0.5479766860580675 2316 4 -0.4258726871000178 2316 5 -0.3689996243321687 2316 6 -0.5396852381463546 2316 7 -0.47895865980824937 2316 8 -0.4789814555088938 2316 9 -0.5085094341949414 2316 10-11 -0.5086377181864421 2316 12-13 -0.4728250763329527 2316 14-15 -0.8635200470264763 2316 16-17 -0.5828277142008176 2316 18-19 -0.4861915076041541 2316 20-21 -0.42467008082459756 2316 22-23 -0.5121192147135574 2316 24-25 -0.6910122392973079 2316 26-27 -0.5375869652499361 2316 28-29 -0.5131807808065076 2316 30-31 -0.5043154032396941 2316 32-33 -0.5923509733186876 2316 34-35 -0.620732488661595 2316 36-37 -0.5683176165554968 2316 38-39 -0.7244890607789216 2316 40-41 -0.4665740793495132 2316 42-43 -0.13473934571103996 2316 44-45 -0.10607376941396751 2316 46-47 -0.11204390165831768 2316 48-49 0.14178892768907048 2316 50-51 0.2514909186273897 2316 52-53 0.17305657873081515 2316 54-55 0.6085893045131456 2316 56-57 0.6507333357120899 2316 58-59 0.7785718977358869 2316 60-61 0.8955822258610944 2316 62-63 0.9457161154441245 2316 64-65 1.1726869929164074 2316 66-67 1.2853443267506997 2316 68-69 1.2276456703431329 2316 70-71 1.4521876678010628 2316 72-73 1.7277929085353634 2316 74-75 2.0214556287228724 2316 76-77 1.9110723012435962 2316 78-79 1.9095248039787087 2316 80-81 1.9971053228496523 2316 82-83 2.457389814361793 2316 84-85 2.699063684873483 2316 86-87 2.808469252466903 2316 88-89 3.118539143197811 2316 90-91 3.2644951885800033 2316 92-93 3.2998667729478335 2316 94-95 3.735758743799984 2316 96-97 3.9973816086507945 2316 98-99 4.166842235786746 2316 100-101 4.368728484062247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 48.0 5 93.0 6 263.0 7 635.0 8 1498.0 9 2673.0 10 4225.0 11 6513.0 12 9307.0 13 12923.0 14 17159.0 15 22135.0 16 27925.0 17 34056.0 18 33930.0 19 31346.0 20 32511.0 21 34869.0 22 37964.0 23 42458.0 24 48659.0 25 55002.0 26 63492.0 27 74681.0 28 86613.0 29 100680.0 30 114783.0 31 132610.0 32 152663.0 33 177849.0 34 211367.0 35 250376.0 36 286113.0 37 276109.0 38 119576.0 39 4663.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.01695918908519 19.818245982012016 15.34381934655483 22.82097548234797 2 16.469070005864072 20.76572030365527 39.02417035332038 23.74103933716027 3 19.841158442001735 24.321771190637783 29.68300274904278 26.1540676183177 4 13.816934067314381 17.138271422789433 35.77878486393371 33.26600964596248 5 13.95357098665645 37.3916410213425 33.898528337584274 14.756259654416784 6 33.06068901440913 36.03403003048507 16.59177321819853 14.313507736907273 7 29.215861806335074 32.20898123244218 20.03672563636835 18.538431324854404 8 26.539954581433417 35.85793837988504 18.909037257978653 18.693069780702892 9 26.397119370280947 16.61159150405637 19.581503199038195 37.40978592662449 10-11 25.414598938904255 26.694473409801876 26.806125724493924 21.084801926799944 12-13 26.30905360706759 23.69447540359321 27.430640983975902 22.5658300053633 14-15 22.758031489940326 25.187924802166055 25.732130146723105 26.32191356117051 16-17 22.43613387910048 28.44360660901951 26.467201135663544 22.65305837621646 18-19 22.44632215281613 26.748604844514183 28.915517079813462 21.88955592285623 20-21 22.928161704452336 25.936035186429457 29.269295414080553 21.866507695037654 22-23 22.652360549249636 25.893726934326505 29.12069814597344 22.333214370450417 24-25 22.53111890070842 26.08737955638108 29.17084785409133 22.210653688819168 26-27 22.16125623130608 26.461575274177466 29.047377866400797 22.329790628115653 28-29 22.234182754901237 26.63761048096228 29.049300478310542 22.078906285825937 30-31 22.39260941428392 26.305305279360073 28.9184878289008 22.383597477455204 32-33 22.181896483831768 26.29987369268943 28.94865862034933 22.56957120312947 34-35 22.708086939580713 26.354086342552574 28.816857330813612 22.120969387053105 36-37 22.780096299225047 26.037557455433845 28.720568950608016 22.461777294733093 38-39 22.329751326677133 26.310331082540223 28.84831084833358 22.51160674244906 40-41 22.661060024620948 26.19720045413561 28.79075426469949 22.35098525654395 42-43 22.486765998690807 26.21545546319154 28.59936023780028 22.698418300317368 44-45 22.49082583898338 26.497369625227673 28.622285759289557 22.389518776499393 46-47 22.778736127194367 26.675343650011325 28.00707716454049 22.53884305825382 48-49 22.68509860295041 26.69305781795489 27.93999485601836 22.68184872307634 50-51 22.84067414072578 26.813801821129 27.833745715840873 22.511778322304345 52-53 22.981954194637897 26.786068183676033 27.6978090227033 22.534168598982767 54-55 22.64727638134848 26.899195903955086 27.772396756500257 22.681130958196178 56-57 22.72866294359365 27.00050044162478 27.708834688779145 22.562001926002427 58-59 22.72904176394707 26.690944399141074 27.771419798746706 22.808594038165154 60-61 22.60823794703294 26.872459162169005 27.89174510574072 22.62755778505733 62-63 22.552013031420156 26.88986496051296 27.93596739752411 22.62215461054277 64-65 22.37133566075242 26.806783675634076 27.913018859272228 22.90886180434128 66-67 22.403476227541855 26.90782589832585 27.721215929971414 22.96748194416088 68-69 22.594503311192256 26.668560770321985 27.825339717459546 22.911596201026203 70-71 22.443630534515517 26.83365998281352 27.62788676062741 23.094822722043556 72-73 22.52138192950069 26.877419841066825 27.455061411800504 23.146136817631984 74-75 22.42867709951212 27.0737523352281 27.28981950207055 23.207751063189228 76-77 22.47275982590214 26.8340188652536 27.626251851733702 23.06696945711056 78-79 22.541146868651023 26.906891938503502 27.57178147249465 22.980179720350826 80-81 22.480480280502572 27.075213201134307 27.4457195185631 22.998586999800022 82-83 22.58675330449615 27.08254286947602 27.39212224467477 22.938581581353063 84-85 22.514196912638816 27.035296233503896 27.314837984420233 23.135668869437055 86-87 22.552410081158488 27.07128608442437 27.232725782799104 23.143578051618036 88-89 22.447485467237584 27.19925416164563 27.03510512476762 23.318155246349164 90-91 22.442941597868536 27.069601371263875 27.241926508502907 23.245530522364685 92-93 22.440872979636563 26.902071514276177 27.22769829398843 23.429357212098832 94-95 22.283210083799048 26.848274473290175 27.247969821974372 23.620545620936404 96-97 22.45118700367057 26.70023546675652 27.18055973697905 23.66801779259386 98-99 22.241380341616207 26.845204034636144 26.94886124607971 23.964554377667945 100-101 22.35427210170652 26.644068850962682 27.199184771935325 23.802474275395475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1257.0 1 860.0 2 718.5 3 985.0 4 777.5 5 669.0 6 1193.5 7 2710.0 8 4176.0 9 4420.5 10 3549.0 11 2620.5 12 2505.0 13 2666.0 14 2884.0 15 3011.0 16 3169.5 17 3379.5 18 3627.5 19 3957.5 20 4342.5 21 5029.5 22 6031.0 23 7279.0 24 8725.0 25 10726.0 26 13329.5 27 16361.5 28 19586.5 29 23026.0 30 26948.0 31 31673.0 32 37929.0 33 42087.0 34 44560.0 35 47853.5 36 52585.5 37 57944.5 38 61798.5 39 64804.5 40 68217.0 41 72972.0 42 76858.5 43 80642.0 44 84875.0 45 88513.5 46 89909.0 47 91532.0 48 93880.0 49 94229.5 50 93232.5 51 90617.5 52 88453.5 53 88016.5 54 88363.5 55 84179.0 56 77142.0 57 71301.0 58 65548.0 59 57897.5 60 48033.0 61 39997.5 62 33562.5 63 26276.5 64 19586.0 65 14972.0 66 11423.0 67 8844.5 68 6845.0 69 5192.5 70 3950.5 71 2922.0 72 2269.0 73 1878.0 74 1430.5 75 1046.0 76 758.0 77 498.0 78 320.5 79 196.5 80 107.5 81 66.5 82 40.0 83 27.0 84 18.0 85 9.0 86 4.5 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.03967644754235311 3 1.1962748002719532E-4 4 0.0 5 0.0978951544889215 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.769577535554284E-4 26-27 0.011364610602583555 28-29 0.0 30-31 0.0 32-33 4.984478334466472E-4 34-35 0.014574614649979964 36-37 0.003210004047396408 38-39 0.011902934262705934 40-41 0.0065396355748200095 42-43 0.0053632986878859226 44-45 0.0024922391672332358 46-47 3.9875826675731775E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.5888244008158596E-4 68-69 9.968956668932944E-5 70-71 0.0 72-73 5.981374001359766E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9937913337865888E-5 82-83 0.0010965852335826237 84-85 0.004366403020992629 86-87 0.0014953435003399415 88-89 1.1962748002719532E-4 90-91 0.0010168335802311602 92-93 2.3925496005439064E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.174544468525012E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2507785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.64290442118296 #Duplication Level Percentage of deduplicated Percentage of total 1 77.05775159120435 42.87717106700642 2 12.928560372483183 14.387652982191504 3 4.20290826073628 7.015860679294475 4 1.8514806919045321 4.120870527092383 5 0.9937212039574711 2.7646766986554208 6 0.6343084520349999 2.1176858742079205 7 0.4093290435532492 1.5943379793073376 8 0.30117537994505356 1.3406618304236861 9 0.22474669576327105 1.125500302019914 >10 1.2718830747829801 12.948951903311132 >50 0.08543207704698344 3.261029277925267 >100 0.034537972217905374 3.45390075153798 >500 0.002728913897472494 1.053642347962328 >1k 0.0012208299014959507 0.9433151891790311 >5k 1.4362704723481774E-4 0.5881584390022518 >10k+ 7.181352361740887E-5 0.40658415088307037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10175 0.4057365364255707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8378 0.3340796758892808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6341 0.2528526169508152 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.987582667573177E-5 0.0 0.0 0.0 0.0 8 3.987582667573177E-5 0.0 0.0 0.0 0.0 9 3.987582667573177E-5 0.0 0.0 0.0 0.0 10-11 3.987582667573177E-5 0.0 0.0 0.0 0.0 12-13 3.987582667573177E-5 0.0 0.0 0.0 0.0 14-15 3.987582667573177E-5 0.0 0.0 3.987582667573177E-5 0.0 16-17 3.987582667573177E-5 0.0 0.0 3.987582667573177E-5 0.0 18-19 3.987582667573177E-5 0.0 0.0 3.987582667573177E-5 0.0 20-21 3.987582667573177E-5 0.0 0.0 7.975165335146354E-5 0.0 22-23 3.987582667573177E-5 0.0 0.0 2.791307867301224E-4 0.0 24-25 7.975165335146354E-5 0.0 0.0 4.3863409343304947E-4 0.0 26-27 7.975165335146354E-5 0.0 0.0 7.177648801631718E-4 0.0 28-29 1.1962748002719532E-4 0.0 0.0 0.0023327358605303087 0.0 30-31 1.1962748002719532E-4 0.0 0.0 0.007616282895064768 0.0 32-33 1.1962748002719532E-4 0.0 0.0 0.020077478731230947 0.0 34-35 1.5950330670292707E-4 0.0 0.0 0.03788203534194519 0.0 36-37 1.9937913337865886E-4 0.0 0.0 0.06924437302240821 0.0 38-39 1.9937913337865886E-4 0.0 0.0 0.11779319200011165 0.0 40-41 1.9937913337865886E-4 0.0 0.0 0.1720641921057826 0.0 42-43 2.1931704671652476E-4 0.0 0.0 0.23084116062581123 0.0 44-45 2.3925496005439064E-4 0.0 0.0 0.3038139234424004 0.0 46-47 2.3925496005439064E-4 0.0 0.0 0.3921189416158084 0.0 48-49 2.3925496005439064E-4 0.0 0.0 0.48570750682375086 0.0 50-51 2.3925496005439064E-4 0.0 0.0 0.5878494368536378 0.0 52-53 2.3925496005439064E-4 0.0 0.0 0.6885159612965226 0.0 54-55 2.3925496005439064E-4 0.0 0.0 0.8018031848822766 0.0 56-57 2.3925496005439064E-4 0.0 0.0 0.9397536072669707 0.0 58-59 2.3925496005439064E-4 0.0 0.0 1.0864767115203258 0.0 60-61 2.3925496005439064E-4 0.0 0.0 1.2518218268312475 0.0 62-63 2.3925496005439064E-4 0.0 0.0 1.4131394836479203 0.0 64-65 2.3925496005439064E-4 0.0 0.0 1.5842665938268232 0.0 66-67 2.3925496005439064E-4 0.0 0.0 1.7798375857579498 0.0 68-69 2.3925496005439064E-4 0.0 0.0 2.0023247606951955 1.9937913337865884E-5 70-71 2.591928733922565E-4 0.0 0.0 2.2426763059831685 3.987582667573177E-5 72-73 2.791307867301224E-4 0.0 0.0 2.4860584140984976 3.987582667573177E-5 74-75 2.791307867301224E-4 0.0 0.0 2.7342455593282518 3.987582667573177E-5 76-77 2.791307867301224E-4 0.0 0.0 3.0068167725702164 3.987582667573177E-5 78-79 2.791307867301224E-4 0.0 0.0 3.3113683988061178 3.987582667573177E-5 80-81 3.1900661340585415E-4 0.0 0.0 3.638928377033916 3.987582667573177E-5 82-83 3.5888244008158596E-4 0.0 0.0 3.9816611073118313 3.987582667573177E-5 84-85 3.5888244008158596E-4 0.0 0.0 4.318252960281683 3.987582667573177E-5 86-87 3.5888244008158596E-4 0.0 0.0 4.66618948594078 5.981374001359765E-5 88-89 3.5888244008158596E-4 0.0 0.0 5.0511905924949705 7.975165335146354E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4505 0.0 56.58606 1 GTATCAA 8085 0.0 43.000824 1 ATCAACG 10925 0.0 31.501495 3 TCAACGC 11160 0.0 30.880753 4 TATCAAC 11170 0.0 30.783964 2 CAACGCA 11475 0.0 29.99162 5 AACGCAG 11850 0.0 29.098358 6 GTGGTAT 2045 0.0 26.97765 1 TGGTATC 2020 0.0 25.898867 2 ACGCAGA 13645 0.0 25.06162 7 CGCAGAG 13845 0.0 24.699589 8 GTACATG 9860 0.0 22.429289 1 GCAGAGT 15125 0.0 22.232489 9 TACATGG 9760 0.0 22.074345 2 AGTACTT 9540 0.0 20.785397 12-13 ACATGGG 9965 0.0 20.75038 3 GAGTACT 9105 0.0 20.70908 12-13 AGAGTAC 14195 0.0 20.560644 10-11 GTAAGGT 1070 0.0 19.99138 4 CAGAGTA 14650 0.0 19.67892 10-11 >>END_MODULE