FastQCFastQC Report
Thu 26 May 2016
SRR938332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456697
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96050.39097210604319543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71280.29014567119998924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52780.2148413092864118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.055.3349881
GTATCAA82850.045.055881
ATCAACG104150.035.6862223
TCAACGC106400.034.797574
TATCAAC107800.034.4779242
CAACGCA109100.033.8921475
AACGCAG112900.032.784156
GTGGTAT20100.028.9364971
ACGCAGA128450.028.6603767
CGCAGAG130600.028.0794378
TAGACCG1903.929017E-1027.5158755
TGGTATC19550.027.4716532
GTACATG99900.025.865211
TACATGG98650.025.4384082
GCAGAGT143350.025.348979
ACATGGG102000.023.9039823
GAGTACT87250.022.21150812-13
AGAGTAC134250.021.7238810-11
AGTACTT90750.021.43337812-13
CAGAGTA138950.021.19417410-11
Produced by FastQC (version 0.11.5)