##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938332_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2456697 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39573174876674 31.0 31.0 34.0 30.0 34.0 2 31.5792464434971 33.0 31.0 34.0 30.0 34.0 3 31.63977893895747 33.0 31.0 34.0 30.0 34.0 4 35.31619161825817 37.0 35.0 37.0 33.0 37.0 5 35.30953104920957 37.0 35.0 37.0 33.0 37.0 6 35.30021813841918 37.0 35.0 37.0 33.0 37.0 7 35.22562489391244 37.0 35.0 37.0 32.0 37.0 8 35.18822956188736 37.0 35.0 37.0 32.0 37.0 9 36.71358901810032 39.0 37.0 39.0 32.0 39.0 10-11 36.55518792101753 39.0 37.0 39.0 32.0 39.0 12-13 36.37629894936168 39.0 36.0 39.0 32.0 39.0 14-15 37.58374720203591 40.0 37.0 41.0 32.0 41.0 16-17 37.51869013557635 40.0 37.0 41.0 32.0 41.0 18-19 37.51204768027966 40.0 37.0 41.0 32.0 41.0 20-21 37.44934072048771 40.0 37.0 41.0 32.0 41.0 22-23 37.290090312317716 39.5 37.0 41.0 31.5 41.0 24-25 37.14924673250303 39.0 36.5 41.0 31.0 41.0 26-27 36.93340163642485 39.0 36.0 41.0 31.0 41.0 28-29 36.69298187770002 39.0 36.0 40.5 30.0 41.0 30-31 36.4150908720123 39.0 35.5 40.0 30.0 41.0 32-33 36.119509650559266 38.5 35.0 40.0 29.5 41.0 34-35 35.805779263783855 38.0 35.0 40.0 28.5 41.0 36-37 35.571018933144785 38.0 35.0 40.0 27.0 41.0 38-39 35.32019373980593 38.0 34.0 40.0 26.5 41.0 40-41 35.03375996307237 38.0 34.0 40.0 25.5 41.0 42-43 34.60350971243096 38.0 33.5 40.0 24.5 41.0 44-45 34.11145452613815 38.0 33.0 40.0 23.5 41.0 46-47 33.5951552022899 38.0 32.5 40.0 21.0 41.0 48-49 33.0913718704423 37.0 31.5 40.0 18.5 41.0 50-51 32.370050315525276 36.5 30.5 39.5 15.0 40.5 52-53 32.36617580434218 36.5 31.0 39.0 14.5 40.0 54-55 32.32741624221465 37.0 31.0 39.0 11.0 40.0 56-57 31.886427793089666 36.0 30.5 39.0 8.0 40.5 58-59 31.399182520270102 36.0 30.0 39.0 4.5 40.0 60-61 30.83633411039294 35.0 29.0 39.0 2.0 40.0 62-63 30.085548197437454 35.0 28.0 38.0 2.0 40.0 64-65 29.402768229049002 34.0 27.5 38.0 2.0 40.0 66-67 28.68532220294159 34.0 26.0 37.5 2.0 39.5 68-69 27.802938864662593 33.5 25.0 36.5 2.0 39.0 70-71 26.94483772317058 32.5 22.5 36.0 2.0 39.0 72-73 26.015421926269294 32.0 20.0 35.5 2.0 38.5 74-75 25.05506621288665 31.0 15.5 35.0 2.0 37.5 76-77 24.079190066988318 30.5 7.5 34.5 2.0 37.0 78-79 23.08287753841845 29.5 2.0 34.0 2.0 36.0 80-81 22.102486183684842 29.0 2.0 34.0 2.0 35.5 82-83 21.080596223303075 28.0 2.0 34.0 2.0 35.0 84-85 20.074980756682653 26.5 2.0 33.0 2.0 35.0 86-87 19.057182265456426 25.5 2.0 32.5 2.0 35.0 88-89 17.954420304986737 23.0 2.0 32.0 2.0 34.0 90-91 16.777614821852268 19.0 2.0 31.5 2.0 34.0 92-93 15.59907774544439 6.0 2.0 31.0 2.0 34.0 94-95 14.334080474718697 2.0 2.0 30.5 2.0 34.0 96-97 12.903331587086239 2.0 2.0 29.5 2.0 34.0 98-99 11.199285666893394 2.0 2.0 28.0 2.0 34.0 100-101 7.881761364954652 2.0 2.0 9.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5556128156131308 1101 2 0.3401512016559707 1101 3 0.41225850655552776 1101 4 0.3099434538204662 1101 5 0.3208813046314134 1101 6 0.3992377708155601 1101 7 0.22453842202632046 1101 8 0.3642697546620184 1101 9 0.41706912571015664 1101 10-11 0.4824240147130112 1101 12-13 0.412876832433831 1101 14-15 0.44003470879149376 1101 16-17 0.5536840798991207 1101 18-19 0.48590427908504097 1101 20-21 0.6232624819558126 1101 22-23 0.6839129481352089 1101 24-25 0.7541313444469537 1101 26-27 0.766209587551451 1101 28-29 0.764239653040292 1101 30-31 0.8710077631655437 1101 32-33 0.8105193003536755 1101 34-35 0.8378198378557542 1101 36-37 1.0222122322779654 1101 38-39 1.0692207714014828 1101 40-41 0.9788439555350976 1101 42-43 1.1980217175170722 1101 44-45 1.3698997577212992 1101 46-47 1.391605958046334 1101 48-49 1.1841668457737597 1101 50-51 1.4716736680734996 1101 52-53 1.4744818384118261 1101 54-55 1.6227296019592359 1101 56-57 1.9091588075890407 1101 58-59 1.6804788457181274 1101 60-61 1.8111337529781615 1101 62-63 1.8108745187025121 1101 64-65 1.8473827342257216 1101 66-67 2.0890956359556228 1101 68-69 2.2394657067225587 1101 70-71 2.7837725049016093 1101 72-73 2.8544352004730165 1101 74-75 2.787572548102599 1101 76-77 3.16915933583671 1101 78-79 3.2632819123450183 1101 80-81 3.5821984540690295 1101 82-83 3.7036019012210453 1101 84-85 3.7452868775323402 1101 86-87 3.8911456910125395 1101 88-89 4.311329573263325 1101 90-91 4.2021708105267095 1101 92-93 4.309028116682246 1101 94-95 4.422843942158904 1101 96-97 4.016211012868842 1101 98-99 3.4164808600481624 1101 100-101 2.2806374335291535 1102 1 0.6806532249525148 1102 2 0.536109963602005 1102 3 0.4874694486291453 1102 4 0.32184592399814704 1102 5 0.4252578299604295 1102 6 0.502103602685338 1102 7 0.4908168853309931 1102 8 0.5379824720095243 1102 9 0.57502577405387 1102 10-11 0.7175179483702223 1102 12-13 0.7291252643385846 1102 14-15 0.732453947134367 1102 16-17 0.8090559750705282 1102 18-19 0.8181646171093533 1102 20-21 0.6546665069236894 1102 22-23 0.5905749010125234 1102 24-25 0.6574218735317956 1102 26-27 0.7929330477769483 1102 28-29 0.7157813662746264 1102 30-31 0.5697853711455281 1102 32-33 0.8520145599542275 1102 34-35 0.993123839254686 1102 36-37 1.0326998053607142 1102 38-39 1.2377611693365367 1102 40-41 1.2300972913013482 1102 42-43 1.524778858527803 1102 44-45 1.401622115528319 1102 46-47 1.58979875203093 1102 48-49 1.6480264508798612 1102 50-51 1.8606802749814833 1102 52-53 1.7458678445397524 1102 54-55 1.7491351855187531 1102 56-57 2.0359221069374804 1102 58-59 2.0348939937082946 1102 60-61 2.210429269556254 1102 62-63 2.27276322765643 1102 64-65 2.415654826311876 1102 66-67 2.555671676220509 1102 68-69 2.652931026157816 1102 70-71 2.6751991864699285 1102 72-73 2.7402516363920917 1102 74-75 2.6401676411999375 1102 76-77 2.9551355083918125 1102 78-79 3.3445176854444902 1102 80-81 3.585810053031995 1102 82-83 3.5091399194527675 1102 84-85 3.5952290195694587 1102 86-87 3.7537416964562063 1102 88-89 3.842952333263021 1102 90-91 3.8391446337900206 1102 92-93 3.9730184153996397 1102 94-95 3.7918957862389195 1102 96-97 3.8437155936076923 1102 98-99 3.3120660161734268 1102 100-101 2.2558201688954993 1103 1 0.4093223209094816 1103 2 0.4030945504340444 1103 3 0.2628806550509175 1103 4 0.19272295815154905 1103 5 0.27104512371594325 1103 6 0.20416065328549138 1103 7 0.3265113077709927 1103 8 0.28461911204453116 1103 9 0.26292775747132424 1103 10-11 0.44964893389256133 1103 12-13 0.30078902641901806 1103 14-15 0.3477063443610575 1103 16-17 0.38784487577894566 1103 18-19 0.3713074548523707 1103 20-21 0.410513476723537 1103 22-23 0.4596720650801487 1103 24-25 0.3632672590118844 1103 26-27 0.4697905872529162 1103 28-29 0.4989767883449616 1103 30-31 0.5359630111126421 1103 32-33 0.7116383217328917 1103 34-35 0.7087582084313624 1103 36-37 0.6630174991946873 1103 38-39 0.6404277303818873 1103 40-41 0.7830203612603839 1103 42-43 0.8573785763679993 1103 44-45 0.8911036657474796 1103 46-47 1.2059399526435328 1103 48-49 1.0501253727879813 1103 50-51 1.2925684823613963 1103 52-53 1.2988216201246345 1103 54-55 1.2649104837103664 1103 56-57 1.5514027970973423 1103 58-59 1.6596329958886287 1103 60-61 1.6419629665286983 1103 62-63 1.8268710670214965 1103 64-65 1.8339110183616079 1103 66-67 2.0018223073078794 1103 68-69 2.21751785819729 1103 70-71 2.4336940826969844 1103 72-73 2.642468552400512 1103 74-75 2.55130329669338 1103 76-77 2.699777414403929 1103 78-79 2.7263111354139333 1103 80-81 2.752062306915466 1103 82-83 3.3261365772925053 1103 84-85 3.256948534616722 1103 86-87 3.4815133884342693 1103 88-89 3.868907942132193 1103 90-91 3.857195090602218 1103 92-93 3.9073417222521982 1103 94-95 3.8554487364686434 1103 96-97 3.9809489940860043 1103 98-99 3.566341642545561 1103 100-101 2.1764750965071746 1104 1 0.3512514515042824 1104 2 0.3315914173299923 1104 3 0.3018993224895148 1104 4 0.2631273027917018 1104 5 0.3274908760451041 1104 6 0.36813082886243365 1104 7 0.36415021866432085 1104 8 0.23742296158114584 1104 9 0.3688261986823207 1104 10-11 0.33590061353820744 1104 12-13 0.5134115138702455 1104 14-15 0.4602383857505785 1104 16-17 0.5648034460334372 1104 18-19 0.5644853364798976 1104 20-21 0.4636686300659463 1104 22-23 0.4484842303493508 1104 24-25 0.4829245586822921 1104 26-27 0.5473941443174724 1104 28-29 0.6613427500638167 1104 30-31 0.5248047292072329 1104 32-33 0.526250853197638 1104 34-35 0.5853131735871457 1104 36-37 0.6632905384692407 1104 38-39 0.7996632550115734 1104 40-41 0.8544509818221471 1104 42-43 0.8242892869029745 1104 44-45 0.7809114873279981 1104 46-47 1.0583512685789458 1104 48-49 1.1649903574615976 1104 50-51 1.3398922145343732 1104 52-53 1.3100254566291767 1104 54-55 1.5368834058259893 1104 56-57 1.5856723254962368 1104 58-59 1.7657907090823457 1104 60-61 1.794540672079048 1104 62-63 1.8406937884157664 1104 64-65 1.995824677691921 1104 66-67 2.073188848694592 1104 68-69 2.4609190366486366 1104 70-71 2.6317147353646497 1104 72-73 2.7362988450844874 1104 74-75 2.492691682663658 1104 76-77 2.591356315185866 1104 78-79 2.913847022274762 1104 80-81 3.2956277125798934 1104 82-83 3.3568818952239674 1104 84-85 3.454686993841971 1104 86-87 3.4586411594261044 1104 88-89 3.541812080983764 1104 90-91 3.240419768047545 1104 92-93 3.2511700327344712 1104 94-95 3.3924128338222825 1104 96-97 3.262592826029321 1104 98-99 2.8404961398152384 1104 100-101 2.2221958401831214 1105 1 0.40780160980534674 1105 2 0.29399266435414617 1105 3 0.2733709311733783 1105 4 0.10914299803840066 1105 5 0.2101906589678606 1105 6 0.3549160890989711 1105 7 0.27184988389626596 1105 8 0.27782247896573864 1105 9 0.3584604073306821 1105 10-11 0.3823377388141225 1105 12-13 0.43470502174043446 1105 14-15 0.42176789000641435 1105 16-17 0.4985742568843605 1105 18-19 0.5409797121052193 1105 20-21 0.46542430006311264 1105 22-23 0.4895049475322395 1105 24-25 0.5633020526544428 1105 26-27 0.3574067663109233 1105 28-29 0.38041464308868456 1105 30-31 0.5475741629371953 1105 32-33 0.6043396762535167 1105 34-35 0.5091287955609332 1105 36-37 0.4818115831094971 1105 38-39 0.6214379659975506 1105 40-41 0.816619616417654 1105 42-43 0.9743132265987597 1105 44-45 0.9876166375056243 1105 46-47 0.9275384155238271 1105 48-49 0.9251965739179866 1105 50-51 0.7032371203360341 1105 52-53 0.9285894653388524 1105 54-55 1.211810721365019 1105 56-57 1.2691532707999222 1105 58-59 1.2632088495841316 1105 60-61 1.4733804455920811 1105 62-63 1.5380369114093106 1105 64-65 1.7844457484456981 1105 66-67 1.9254025574865423 1105 68-69 2.0101254959634787 1105 70-71 2.1403424411110343 1105 72-73 2.2713611533696145 1105 74-75 2.1342170303569326 1105 76-77 2.1903844106134507 1105 78-79 2.5291253111998913 1105 80-81 2.6405220220768193 1105 82-83 3.01460316018143 1105 84-85 3.134813820230299 1105 86-87 3.219442752932011 1105 88-89 3.395726560470532 1105 90-91 3.193474010773631 1105 92-93 3.053303185627019 1105 94-95 3.136603407646687 1105 96-97 2.9509109329407632 1105 98-99 2.921192575888222 1105 100-101 2.08104994151408 1106 1 0.1748896803547204 1106 2 0.16565304279267679 1106 3 -0.019552749771857947 1106 4 0.1144976726247009 1106 5 0.1563248718113357 1106 6 -0.15259022017410473 1106 7 0.11304476509337036 1106 8 -0.14001386547763417 1106 9 0.04304221069686065 1106 10-11 0.1134503258770323 1106 12-13 0.2069183770278542 1106 14-15 0.36172457110149736 1106 16-17 -0.07754950083280931 1106 18-19 0.33041750915455026 1106 20-21 0.3643614464029028 1106 22-23 0.32776995215370874 1106 24-25 0.49194329029895556 1106 26-27 0.37110491789173494 1106 28-29 0.5614966728984214 1106 30-31 0.37351398787696155 1106 32-33 0.45503255986489677 1106 34-35 0.39298850493872095 1106 36-37 0.4074610720541756 1106 38-39 0.611194551801141 1106 40-41 0.749164690470586 1106 42-43 0.5054774158550615 1106 44-45 0.7216649937274298 1106 46-47 0.8456677055348791 1106 48-49 0.7866619415225884 1106 50-51 0.8463473436312654 1106 52-53 1.0095093649292295 1106 54-55 0.7126887162704634 1106 56-57 1.2869694962804594 1106 58-59 1.329495360450892 1106 60-61 1.4779025823437841 1106 62-63 1.465328326588942 1106 64-65 1.5312624907899668 1106 66-67 1.676836980611057 1106 68-69 1.7274149245007528 1106 70-71 1.641840840853778 1106 72-73 1.750656299396244 1106 74-75 2.086718179410667 1106 76-77 2.094163155897281 1106 78-79 2.6586447121093677 1106 80-81 2.580987148927303 1106 82-83 2.6148721986970394 1106 84-85 2.5770441966653514 1106 86-87 2.1915464219910668 1106 88-89 2.3011367095192163 1106 90-91 2.1252905089965957 1106 92-93 2.5203215553344265 1106 94-95 2.25003058457739 1106 96-97 2.1947317175971524 1106 98-99 2.0581256883338614 1106 100-101 1.426765275867674 1107 1 0.20347490824149617 1107 2 0.12357464568668064 1107 3 0.0839645556826305 1107 4 0.1173754222998582 1107 5 0.10842465167256421 1107 6 0.1377305993464475 1107 7 0.12310139015903587 1107 8 0.18292214850534805 1107 9 0.3603225599252582 1107 10-11 0.22432285790299744 1107 12-13 0.21015029516738792 1107 14-15 0.31894977532956403 1107 16-17 0.3210203650928136 1107 18-19 0.2770554290428038 1107 20-21 0.28231262826299286 1107 22-23 0.4207226629492169 1107 24-25 0.42791203355314167 1107 26-27 0.38233651935315294 1107 28-29 0.4391468625283039 1107 30-31 0.3772713990248704 1107 32-33 0.5657816600270564 1107 34-35 0.640497945940389 1107 36-37 0.5660409270245239 1107 38-39 0.7773738441232538 1107 40-41 0.6882523223465 1107 42-43 0.9643620386185248 1107 44-45 0.7876131445254231 1107 46-47 0.8127822595199916 1107 48-49 0.802182102106876 1107 50-51 1.050945222446309 1107 52-53 1.0725743903430711 1107 54-55 1.16730362979974 1107 56-57 1.54315840344211 1107 58-59 1.5500086191833518 1107 60-61 1.5607754896919737 1107 62-63 1.7378790069582202 1107 64-65 1.9736825037307675 1107 66-67 2.0744712637274496 1107 68-69 2.0363405841700626 1107 70-71 2.3887829002055163 1107 72-73 2.387789658588833 1107 74-75 2.6836937357127724 1107 76-77 2.521323625418688 1107 78-79 2.8502222048395964 1107 80-81 3.0115228655567883 1107 82-83 2.7888440350085126 1107 84-85 2.797938781664808 1107 86-87 2.819082255629489 1107 88-89 3.3017757905606118 1107 90-91 2.8839864513555895 1107 92-93 2.862032686794109 1107 94-95 2.8786655761703397 1107 96-97 2.7887296198139815 1107 98-99 2.476582815169598 1107 100-101 1.6627538743379544 1108 1 0.25458064774618094 1108 2 0.16199101561735674 1108 3 0.09000957857717751 1108 4 0.10065644415165309 1108 5 0.10970829720962172 1108 6 0.13464640675867656 1108 7 0.06268234069116829 1108 8 0.10559650177181368 1108 9 0.2071502582006417 1108 10-11 0.21055806747721562 1108 12-13 0.13146562989874155 1108 14-15 0.25773043000912566 1108 16-17 0.2351592685810786 1108 18-19 0.16876130842133108 1108 20-21 0.1856980688296872 1108 22-23 0.24479618849544948 1108 24-25 0.24302423832585163 1108 26-27 0.21291693075144735 1108 28-29 0.3951825790265957 1108 30-31 0.35322863271112226 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0.6238718895326514 2316 94-95 0.6666490149640385 2316 96-97 0.7327949421612718 2316 98-99 0.6108642611351272 2316 100-101 0.5129781471310411 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6944.0 3 2326.0 4 2579.0 5 2921.0 6 3627.0 7 4593.0 8 5913.0 9 7647.0 10 10119.0 11 13126.0 12 16734.0 13 20404.0 14 24515.0 15 28710.0 16 33296.0 17 39234.0 18 39331.0 19 38833.0 20 42282.0 21 47217.0 22 53357.0 23 59678.0 24 66905.0 25 74914.0 26 83096.0 27 92511.0 28 102901.0 29 113846.0 30 127087.0 31 142406.0 32 160609.0 33 180870.0 34 202295.0 35 218334.0 36 209259.0 37 143160.0 38 34619.0 39 499.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69599341073648 18.416885314620174 14.32723570426431 23.559885570379038 2 15.728834234928247 19.58169687681442 40.448685636668785 24.240783251588546 3 19.708785027092464 23.66810825909899 28.869690278188926 27.753416435619616 4 12.951028356462157 15.937346528395963 36.18916416157572 34.92246095356616 5 14.081824583869446 37.09318139329149 33.71086181496339 15.114132207875677 6 33.1769443548466 35.378852300384125 16.98087004401698 14.463333300752296 7 29.57282212825842 31.926238052734107 20.033496322164094 18.46744349684338 8 26.67293772714779 35.09537916501237 19.170306121410174 19.06137698642967 9 26.698642486768136 16.548647413851373 19.679136937895134 37.073573161485356 10-11 25.39259346127856 26.354534225175215 26.87594940420019 21.376922909346035 12-13 26.298772024187368 23.545588281399823 27.333261943336158 22.822377751076655 14-15 22.90284484894457 24.990905058172 25.664409166724177 26.441840926159248 16-17 22.609017998295723 28.172623552781417 26.35469675027492 22.863661698647938 18-19 22.61382828162738 26.60129931255975 28.70718563166818 22.077686774144688 20-21 23.044090096392246 25.893306861060882 29.00977766630034 22.05282537624654 22-23 22.73382460843626 25.789642342061352 28.844503204906346 22.63202984459604 24-25 22.52930264320867 25.99198968444341 28.96111244473845 22.517595227609466 26-27 22.212865900932304 26.423210481396303 28.745537197620706 22.618386420050687 28-29 22.275977986648012 26.650919964624887 28.74341806313122 22.32968398559588 30-31 22.423782061291952 26.286213026051737 28.650497587138574 22.639507325517737 32-33 22.096571728476043 26.299959405851503 28.75171161135154 22.851757254320916 34-35 22.737661070681565 26.3904084182856 28.541254493610918 22.330676017421915 36-37 22.766354226894794 26.103508229028392 28.401447103756606 22.728690440320204 38-39 22.366266850544765 26.355376653248996 28.55572575022773 22.722630745978513 40-41 22.720039277134628 26.22821448214067 28.51828149508771 22.533464745636994 42-43 22.467318764734596 26.24354792155445 28.360993967136178 22.928139346574774 44-45 22.46563280101221 26.522627311705037 28.35908634219341 22.65265354508934 46-47 22.741648193045776 26.690829959225717 27.779562373661808 22.7879594740667 48-49 22.684100185464555 26.627344082854272 27.781291469220843 22.907264262460334 50-51 22.782553825955535 26.897518230346083 27.664144356861886 22.655783586836495 52-53 22.8650677161963 26.805139335358056 27.617261467747056 22.71253148069859 54-55 22.595908909663383 26.827952710468967 27.74153470131808 22.83460367854957 56-57 22.58176728095638 27.01072698469368 27.627832394349856 22.779673340000087 58-59 22.636063553110795 26.629992538440465 27.699197790759634 23.034746117689107 60-61 22.49805364312211 26.829987634909248 27.873214567399923 22.798744154568723 62-63 22.5044165160909 26.811117631481295 27.961294648336626 22.723171204091184 64-65 22.299775962144583 26.77184185903181 27.83668826985662 23.091693908966988 66-67 22.328930033304097 26.781152603115398 27.731732967191096 23.158184396389405 68-69 22.595893454273007 26.539160848697247 27.857620154188318 23.007325542841432 70-71 22.464246312678362 26.624233122590468 27.64039912228921 23.27112144244196 72-73 22.50474773401291 26.722323204155618 27.465290146085692 23.307638915745784 74-75 22.43315722999042 26.904038096129828 27.443909677406225 23.218894996473523 76-77 22.61320391614017 26.643243587606445 27.438850762949606 23.30470173330378 78-79 22.634029735059855 26.70623036682251 27.3560270967109 23.30371280140674 80-81 22.661383380037375 26.807529884378745 27.16839977438762 23.362686961196257 82-83 22.66690631637099 26.81444069952202 27.123189854268283 23.39546312983871 84-85 22.702660673817 26.66979580155857 27.122027711976838 23.505515812647594 86-87 22.76370375812926 26.6947118099986 27.02298612186713 23.51859831000501 88-89 22.725435163879563 26.651721521172355 26.93924560195024 23.683597712997837 90-91 22.715557938734655 26.63420467301249 26.880998068892065 23.769239319360793 92-93 22.809886470220416 26.287183771038507 26.994648370329845 23.908281388411233 94-95 22.66193391628919 26.19205624256479 27.018713322677385 24.12729651846864 96-97 22.87709477305459 26.039195729283822 26.825206996402052 24.258502501259535 98-99 22.749634275941677 26.10468816820844 26.52941048355366 24.61626707229622 100-101 23.67074420718226 25.147665653904227 26.724864846139585 24.45672529277393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 962.0 1 722.0 2 663.0 3 848.0 4 735.0 5 681.0 6 1081.0 7 2183.5 8 3487.5 9 3930.5 10 3285.5 11 2507.5 12 2328.0 13 2412.5 14 2549.0 15 2719.0 16 2896.5 17 3105.0 18 3331.0 19 3648.5 20 4162.0 21 4892.5 22 5669.0 23 6736.5 24 8273.0 25 10064.5 26 12472.0 27 15420.0 28 18480.0 29 21889.5 30 25778.0 31 30175.0 32 35760.5 33 39912.5 34 42398.0 35 45865.0 36 50486.0 37 55435.5 38 59193.5 39 62135.5 40 65724.0 41 69931.5 42 73805.0 43 77915.0 44 81863.5 45 85378.5 46 87297.0 47 89035.0 48 90952.0 49 91227.5 50 90528.5 51 88549.0 52 86781.5 53 86402.0 54 86360.0 55 83027.5 56 77185.0 57 71486.0 58 66219.5 59 59491.0 60 50236.5 61 42017.5 62 35255.0 63 28106.5 64 21660.0 65 17001.0 66 13127.0 67 10049.0 68 7698.0 69 5812.0 70 4494.5 71 3367.0 72 2512.0 73 2008.5 74 1567.0 75 1226.0 76 915.0 77 610.5 78 386.0 79 243.0 80 153.0 81 104.5 82 66.0 83 42.0 84 28.5 85 15.5 86 13.5 87 12.0 88 6.0 89 3.5 90 4.0 91 2.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3695612442234431 2 0.02763873607530762 3 0.034517891298764154 4 0.04196691736913424 5 0.06179028182962734 6 0.05214318249259066 7 0.036838079746912215 8 0.039565318799998535 9 0.03594256841604805 10-11 0.03584080576481349 12-13 0.02865636258765326 14-15 0.026926397516665666 16-17 0.021166631456789337 18-19 0.025603483050616334 20-21 0.03231981803209757 22-23 0.03154642188271488 24-25 0.04023695229814666 26-27 0.03423295587530738 28-29 0.0298368093419742 30-31 0.032380875622838305 32-33 0.02759803101481379 34-35 0.028900592950616213 36-37 0.030813730793826016 38-39 0.014633469247530322 40-41 0.01245574851111065 42-43 0.012944209237036557 44-45 0.011600942240740311 46-47 0.02002688976296222 48-49 0.017604938663579595 50-51 0.011580589710493397 52-53 0.009423221504320638 54-55 0.008649825354937952 56-57 0.009301106322839162 58-59 0.004314736412345519 60-61 0.0075100836611108335 62-63 0.011906230194444005 64-65 0.007835724145061437 66-67 0.003765218095678873 68-69 0.006329636906789889 70-71 0.012496453571604475 72-73 0.010664725849382321 74-75 0.01137706440802427 76-77 0.005922586301851633 78-79 0.009626746806789767 80-81 0.011051423924073664 82-83 0.01359549020493777 84-85 0.00871088294567869 86-87 0.0025440662808641035 88-89 0.015508628048147574 90-91 0.024178805933332437 92-93 0.010135560062962588 94-95 0.019497723976542487 96-97 0.01768634878456725 98-99 0.010929308742592188 100-101 0.12260364220740287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2456697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.38303162660603 #Duplication Level Percentage of deduplicated Percentage of total 1 78.08943767421084 44.81008671763121 2 12.50930941277096 14.35644195320073 3 3.959472876451689 6.8161967188234796 4 1.766678081603438 4.05509376922727 5 0.9287876040953552 2.66483242301017 6 0.581208204615229 2.0010893272247157 7 0.3850197979280594 1.5465522268962706 8 0.27704183514865327 1.2718000310582516 9 0.2116369937960356 1.0929935077521962 >10 1.1804853196289142 12.37805690226434 >50 0.07552224411426083 3.0033427344837498 >100 0.03140934687491379 3.166124017326196 >500 0.002707913088203275 1.079130590597927 >1k 0.0010689130611315956 0.858193016478153 >5k 2.137826122263191E-4 0.900066064025379 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9605 0.39097210604319543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7128 0.29014567119998924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5278 0.2148413092864118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.070506049382565E-5 0.0 5 0.0 0.0 0.0 8.14101209876513E-5 0.0 6 0.0 0.0 0.0 8.14101209876513E-5 0.0 7 0.0 0.0 0.0 8.14101209876513E-5 0.0 8 0.0 0.0 0.0 8.14101209876513E-5 0.0 9 0.0 0.0 0.0 8.14101209876513E-5 0.0 10-11 0.0 0.0 0.0 8.14101209876513E-5 0.0 12-13 0.0 0.0 0.0 1.628202419753026E-4 4.070506049382565E-5 14-15 0.0 0.0 0.0 1.8317277222221543E-4 8.14101209876513E-5 16-17 0.0 0.0 0.0 2.0352530246912828E-4 8.14101209876513E-5 18-19 0.0 0.0 0.0 2.0352530246912828E-4 8.14101209876513E-5 20-21 0.0 0.0 0.0 3.052879537036924E-4 8.14101209876513E-5 22-23 0.0 0.0 0.0 4.0705060493825655E-4 8.14101209876513E-5 24-25 4.070506049382565E-5 0.0 0.0 6.105759074073848E-4 8.14101209876513E-5 26-27 4.070506049382565E-5 0.0 0.0 8.751588006172515E-4 8.14101209876513E-5 28-29 8.14101209876513E-5 0.0 0.0 0.002238778327160411 1.0176265123456414E-4 30-31 1.2211518148147696E-4 0.0 0.0 0.008222422219752782 1.2211518148147696E-4 32-33 1.2211518148147696E-4 0.0 0.0 0.019395961325307923 1.2211518148147696E-4 34-35 1.2211518148147696E-4 0.0 0.0 0.03691948986789987 1.2211518148147696E-4 36-37 1.628202419753026E-4 0.0 0.0 0.06594219799999755 1.2211518148147696E-4 38-39 1.628202419753026E-4 0.0 0.0 0.11259019732592176 1.2211518148147696E-4 40-41 1.628202419753026E-4 0.0 0.0 0.16760308658332712 1.2211518148147696E-4 42-43 2.0352530246912828E-4 0.0 0.0 0.2222903353567819 1.2211518148147696E-4 44-45 2.442303629629539E-4 0.0 0.0 0.29161105337776694 1.2211518148147696E-4 46-47 2.442303629629539E-4 0.0 0.0 0.37589088113023295 1.2211518148147696E-4 48-49 2.442303629629539E-4 0.0 0.0 0.4711814277462788 1.2211518148147696E-4 50-51 2.442303629629539E-4 0.0 0.0 0.5741448782654108 1.2211518148147696E-4 52-53 2.442303629629539E-4 0.0 0.0 0.6702495260913333 1.2211518148147696E-4 54-55 2.442303629629539E-4 0.0 0.0 0.7798886065314525 1.2211518148147696E-4 56-57 2.442303629629539E-4 0.0 0.0 0.9103279728839169 1.2211518148147696E-4 58-59 2.442303629629539E-4 0.0 0.0 1.0561742046332943 1.2211518148147696E-4 60-61 2.442303629629539E-4 0.0 0.0 1.217793647324029 1.2211518148147696E-4 62-63 2.442303629629539E-4 0.0 0.0 1.3824863220820476 1.2211518148147696E-4 64-65 2.442303629629539E-4 0.0 0.0 1.549539890348708 1.2211518148147696E-4 66-67 2.442303629629539E-4 0.0 0.0 1.7439879643277132 1.2211518148147696E-4 68-69 2.442303629629539E-4 2.0352530246912826E-5 0.0 1.964609392204248 1.628202419753026E-4 70-71 2.442303629629539E-4 4.070506049382565E-5 0.0 2.20232694548819 1.628202419753026E-4 72-73 2.442303629629539E-4 4.070506049382565E-5 0.0 2.4464758983301564 1.628202419753026E-4 74-75 2.442303629629539E-4 4.070506049382565E-5 0.0 2.6899532176739744 1.628202419753026E-4 76-77 2.442303629629539E-4 4.070506049382565E-5 0.0 2.954454700762853 1.628202419753026E-4 78-79 2.442303629629539E-4 4.070506049382565E-5 0.0 3.2544917016628423 1.628202419753026E-4 80-81 3.256404839506052E-4 4.070506049382565E-5 0.0 3.574453829674559 1.628202419753026E-4 82-83 3.256404839506052E-4 4.070506049382565E-5 0.0 3.9074415770443 1.628202419753026E-4 84-85 3.256404839506052E-4 4.070506049382565E-5 0.0 4.237417149937498 1.628202419753026E-4 86-87 3.256404839506052E-4 4.070506049382565E-5 0.0 4.578627319526991 1.628202419753026E-4 88-89 3.256404839506052E-4 4.070506049382565E-5 0.0 4.943588891914631 1.628202419753026E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4325 0.0 55.334988 1 GTATCAA 8285 0.0 45.05588 1 ATCAACG 10415 0.0 35.686222 3 TCAACGC 10640 0.0 34.79757 4 TATCAAC 10780 0.0 34.477924 2 CAACGCA 10910 0.0 33.892147 5 AACGCAG 11290 0.0 32.78415 6 GTGGTAT 2010 0.0 28.936497 1 ACGCAGA 12845 0.0 28.660376 7 CGCAGAG 13060 0.0 28.079437 8 TAGACCG 190 3.929017E-10 27.515875 5 TGGTATC 1955 0.0 27.471653 2 GTACATG 9990 0.0 25.86521 1 TACATGG 9865 0.0 25.438408 2 GCAGAGT 14335 0.0 25.34897 9 ACATGGG 10200 0.0 23.903982 3 GAGTACT 8725 0.0 22.211508 12-13 AGAGTAC 13425 0.0 21.72388 10-11 AGTACTT 9075 0.0 21.433378 12-13 CAGAGTA 13895 0.0 21.194174 10-11 >>END_MODULE