FastQCFastQC Report
Thu 26 May 2016
SRR938332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456697
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99750.40603297842591085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81400.3313391924197408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62220.2532668863925832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.060.0397641
GTATCAA76550.044.0097271
ATCAACG102050.032.7206543
TATCAAC104650.032.045842
TCAACGC105300.031.7552194
CAACGCA108850.030.7631995
AACGCAG113050.029.662316
GTGGTAT18400.027.3738351
TGGTATC18000.025.861862
ACGCAGA132150.025.2313657
CGCAGAG133500.024.940648
GTACATG96750.024.5073991
TACATGG96350.023.8123112
GCAGAGT146550.022.3307959
ACATGGG97900.022.220943
GGACCGA6450.022.0918666
AGAGTAC137850.020.4323710-11
GAGTACT92600.020.28646312-13
AGTACTT96450.020.11688212-13
GTACTTT94550.019.84295714-15
Produced by FastQC (version 0.11.5)