##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938332_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2456697 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.594130655917276 31.0 31.0 34.0 30.0 34.0 2 31.87064990106635 31.0 31.0 34.0 30.0 34.0 3 31.917240099206374 31.0 31.0 34.0 30.0 34.0 4 35.61480841959753 37.0 35.0 37.0 33.0 37.0 5 35.5030502337081 37.0 35.0 37.0 33.0 37.0 6 35.54683951663555 37.0 35.0 37.0 33.0 37.0 7 35.461037726671215 37.0 35.0 37.0 33.0 37.0 8 35.42816472686701 37.0 35.0 37.0 33.0 37.0 9 36.92542263046684 39.0 37.0 39.0 33.0 39.0 10-11 36.83932776406696 39.0 37.0 39.0 32.5 39.0 12-13 36.700823911129454 39.0 35.0 39.0 32.0 39.0 14-15 37.608225800739774 39.0 36.5 41.0 32.0 41.0 16-17 37.706440802427 39.0 37.0 41.0 32.0 41.0 18-19 37.84990782339051 39.5 37.0 41.0 32.5 41.0 20-21 37.7922564321119 40.0 37.0 41.0 32.0 41.0 22-23 37.638555751889626 39.0 37.0 41.0 32.0 41.0 24-25 37.48474801735827 39.0 36.0 41.0 32.0 41.0 26-27 37.21966669068265 39.0 36.0 41.0 31.0 41.0 28-29 37.048064942481716 39.0 36.0 40.0 31.0 41.0 30-31 36.734079945552914 39.0 35.5 40.0 30.0 41.0 32-33 36.579053908560965 38.5 35.0 40.0 30.0 41.0 34-35 36.4017788925537 38.0 35.0 40.0 30.0 41.0 36-37 36.22737826439321 38.0 35.0 40.0 30.0 41.0 38-39 35.95954832850775 38.0 35.0 40.0 29.0 41.0 40-41 35.680780739342296 38.0 34.5 40.0 28.5 41.0 42-43 35.3475412311734 38.0 34.0 40.0 27.0 41.0 44-45 34.97793582195932 38.0 33.5 40.0 26.0 41.0 46-47 34.46822990380987 38.0 33.0 40.0 24.5 41.0 48-49 33.96053664737654 37.5 33.0 40.0 23.5 41.0 50-51 33.483758884388266 37.0 32.0 40.0 22.5 41.0 52-53 32.76042812768526 36.5 31.0 39.0 19.5 40.0 54-55 32.210407510572125 36.0 30.5 39.0 17.0 40.0 56-57 31.71051130847638 35.0 30.0 39.0 13.5 40.0 58-59 31.042055450875708 35.0 28.5 38.0 8.5 40.0 60-61 30.40753886213888 34.0 28.0 38.0 7.5 40.0 62-63 29.733901250337343 34.0 27.5 38.0 4.0 40.0 64-65 28.92825387094949 33.5 26.0 37.0 2.0 39.0 66-67 28.13546562722224 33.0 25.5 36.5 2.0 39.0 68-69 27.257719612960003 32.0 23.5 36.0 2.0 39.0 70-71 26.35553936850983 31.0 21.5 35.0 2.0 38.0 72-73 25.644699570195264 31.0 20.0 35.0 2.0 37.5 74-75 24.819426856466222 30.0 18.0 34.0 2.0 37.0 76-77 23.814693264981393 29.5 17.0 33.5 2.0 35.5 78-79 24.20213970220992 30.0 14.5 34.0 2.0 36.0 80-81 23.715200327919966 30.0 7.5 34.0 2.0 36.0 82-83 22.898708509840652 29.5 4.5 34.0 2.0 35.0 84-85 21.97377271189732 29.0 2.0 34.0 2.0 35.0 86-87 20.89530088570141 27.5 2.0 33.0 2.0 35.0 88-89 19.753062546988904 26.0 2.0 32.5 2.0 34.5 90-91 18.722413468164774 25.0 2.0 32.0 2.0 34.0 92-93 17.489220282354722 21.5 2.0 32.0 2.0 34.0 94-95 16.18959745544526 12.5 2.0 31.0 2.0 34.0 96-97 14.74191383797025 2.0 2.0 31.0 2.0 34.0 98-99 13.021606449635424 2.0 2.0 30.0 2.0 34.0 100-101 9.694028201280013 2.0 2.0 22.5 2.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3287852859875997 1101 2 0.34442303187726253 1101 3 0.2811775928414626 1101 4 0.19914803102224 1101 5 0.21355352891568913 1101 6 0.21981499244616742 1101 7 0.2936147612849638 1101 8 0.3963743887459614 1101 9 0.32789448860437886 1101 10-11 0.3377312246055624 1101 12-13 0.336161494312087 1101 14-15 0.4884301242278397 1101 16-17 0.5141295344497578 1101 18-19 0.5104289980011956 1101 20-21 0.5285048721123502 1101 22-23 0.42241079344845645 1101 24-25 0.38079868313743503 1101 26-27 0.5572526940170732 1101 28-29 0.6024530662001553 1101 30-31 0.7133752571818164 1101 32-33 0.6481230175859309 1101 34-35 0.522177114948299 1101 36-37 0.6622717219960492 1101 38-39 0.788275462782515 1101 40-41 0.7887455780958774 1101 42-43 0.906315205850035 1101 44-45 0.9778852857524498 1101 46-47 1.1206206486608536 1101 48-49 1.1910355297199047 1101 50-51 1.3995787231437973 1101 52-53 1.3853868400634113 1101 54-55 1.3186245511385266 1101 56-57 1.388232386343084 1101 58-59 1.5940247255419457 1101 60-61 1.6767946696606657 1101 62-63 1.87513516537172 1101 64-65 1.9205657933398292 1101 66-67 2.005531993367846 1101 68-69 2.232538464610762 1101 70-71 2.5578824527258597 1101 72-73 2.5623254013424024 1101 74-75 2.5479592008424063 1101 76-77 2.5355046543183555 1101 78-79 2.673750054606419 1101 80-81 2.824749497221969 1101 82-83 3.1162084934502374 1101 84-85 3.248027770175238 1101 86-87 3.0661213065001434 1101 88-89 3.1237103381043596 1101 90-91 3.4224362535694546 1101 92-93 3.6158320113368703 1101 94-95 3.5434247995148986 1101 96-97 3.6850328109940165 1101 98-99 3.5137050087185333 1101 100-101 2.5384746021841043 1102 1 0.37215093158667045 1102 2 0.3517611854403526 1102 3 0.34174324756399344 1102 4 0.2510299734075687 1102 5 0.29453354092824924 1102 6 0.31388641395777483 1102 7 0.38593234904127627 1102 8 0.2597303026508797 1102 9 0.40904222028332526 1102 10-11 0.48737571365360566 1102 12-13 0.4732006263666122 1102 14-15 0.5794530165177534 1102 16-17 0.4910490731066304 1102 18-19 0.48272985940818813 1102 20-21 0.4651654564701744 1102 22-23 0.6037770320726352 1102 24-25 0.43695496806319056 1102 26-27 0.5417118627175057 1102 28-29 0.5102840388373693 1102 30-31 0.5805985311366086 1102 32-33 0.5202132030203188 1102 34-35 0.6292877901606104 1102 36-37 0.6994438285276914 1102 38-39 0.7490836495702169 1102 40-41 0.8487382032963993 1102 42-43 0.9210736241456203 1102 44-45 0.9805458396987206 1102 46-47 0.9932846239058577 1102 48-49 1.1526953157747002 1102 50-51 1.2340274758903362 1102 52-53 1.2412874399311207 1102 54-55 1.2064960471584243 1102 56-57 1.4047957297882618 1102 58-59 1.8236242055045402 1102 60-61 1.727250614737418 1102 62-63 1.9262435901297579 1102 64-65 1.9297283354408101 1102 66-67 2.106505923071559 1102 68-69 2.3172960465888828 1102 70-71 2.4505021031451584 1102 72-73 2.54310811846182 1102 74-75 2.65100377656335 1102 76-77 2.565088168220232 1102 78-79 2.807297029037912 1102 80-81 3.105290670651403 1102 82-83 3.0715015466932485 1102 84-85 3.3297115053210966 1102 86-87 3.151788269679365 1102 88-89 3.0058625630889644 1102 90-91 3.10603827289599 1102 92-93 3.3538531321543346 1102 94-95 3.170327839909035 1102 96-97 3.230082723940974 1102 98-99 2.880483057665913 1102 100-101 2.4179116845005524 1103 1 0.20437871150202014 1103 2 0.21747439338609098 1103 3 0.15122245902541565 1103 4 0.1914265060106075 1103 5 0.11923368955253011 1103 6 0.16668258744832798 1103 7 0.26561729574023474 1103 8 0.2142864290645079 1103 9 0.24871901905208205 1103 10-11 0.38095250412354176 1103 12-13 0.3377735767472245 1103 14-15 0.47613537549443663 1103 16-17 0.30332038409351014 1103 18-19 0.36140823495232866 1103 20-21 0.36490494903518567 1103 22-23 0.4712070390889025 1103 24-25 0.34609739840970377 1103 26-27 0.528363365180752 1103 28-29 0.437238599037741 1103 30-31 0.4801239142024869 1103 32-33 0.5043462406139625 1103 34-35 0.5902119766878542 1103 36-37 0.566044029208193 1103 38-39 0.6323770731343288 1103 40-41 0.6932231107645848 1103 42-43 0.8883350897168398 1103 44-45 0.8256624380887558 1103 46-47 0.7661028269854668 1103 48-49 0.9465628666426724 1103 50-51 0.9900195241140111 1103 52-53 1.1986183137966364 1103 54-55 1.2092131555485253 1103 56-57 1.3861131293914255 1103 58-59 1.3764334307721775 1103 60-61 1.2615321257258039 1103 62-63 1.2893060047839633 1103 64-65 1.5781689875770049 1103 66-67 1.6581547472799656 1103 68-69 1.6187130320055978 1103 70-71 1.8144054898172577 1103 72-73 1.9205876166096765 1103 74-75 1.760092868144575 1103 76-77 1.9030364741297454 1103 78-79 2.0427925816260135 1103 80-81 2.21867289156593 1103 82-83 2.304132950928661 1103 84-85 2.2773710123713222 1103 86-87 2.2122648481522376 1103 88-89 2.483666243237437 1103 90-91 2.538910720066472 1103 92-93 2.6133565610749585 1103 94-95 2.5118933741642913 1103 96-97 2.5946990545055186 1103 98-99 2.281968923270332 1103 100-101 1.8801605132355856 1104 1 0.1892209952809374 1104 2 0.21676180989395988 1104 3 0.19804148660483278 1104 4 0.29944511016291386 1104 5 0.19234045987416692 1104 6 0.2187246804745726 1104 7 0.2436168750809955 1104 8 0.21574129303429856 1104 9 0.32620969873967454 1104 10-11 0.29098880220112733 1104 12-13 0.2573320114046993 1104 14-15 0.4211602726686223 1104 16-17 0.38673409870922626 1104 18-19 0.4172771305459477 1104 20-21 0.37680652053910535 1104 22-23 0.4227654172356168 1104 24-25 0.4386484862347899 1104 26-27 0.5503680385636471 1104 28-29 0.40059251891729275 1104 30-31 0.5372826825041628 1104 32-33 0.7494563652533728 1104 34-35 0.5731144418403886 1104 36-37 0.6426272929962806 1104 38-39 0.7639931037977661 1104 40-41 0.7669460214555173 1104 42-43 0.8210636328879701 1104 44-45 0.8733246582325052 1104 46-47 0.9623167208210575 1104 48-49 0.9570602464603439 1104 50-51 0.9615871112094325 1104 52-53 0.9000547685186078 1104 54-55 1.1664623266638472 1104 56-57 1.3100061706524997 1104 58-59 1.3976631714093735 1104 60-61 1.3555308695144888 1104 62-63 1.4920085457935528 1104 64-65 1.5973166956256506 1104 66-67 1.8253144655968505 1104 68-69 1.7979205001919816 1104 70-71 2.0403347495795323 1104 72-73 2.236100500127865 1104 74-75 2.195770855369396 1104 76-77 2.293267261513602 1104 78-79 2.411905496628769 1104 80-81 2.6743677282260236 1104 82-83 2.6101652159826187 1104 84-85 2.9106107185095595 1104 86-87 2.920082262435706 1104 88-89 2.716578186918113 1104 90-91 3.0644284315072063 1104 92-93 3.2126092205769616 1104 94-95 2.9334488590173144 1104 96-97 2.9621034558005945 1104 98-99 2.8021953094456062 1104 100-101 2.11394765302809 1105 1 0.22220266846767345 1105 2 0.1917933579545945 1105 3 0.22506745085950186 1105 4 0.18435374193937548 1105 5 0.08318707773099732 1105 6 0.042125186185067776 1105 7 0.21351114996297582 1105 8 0.1627579970989501 1105 9 0.3495743710593757 1105 10-11 0.29685312767907845 1105 12-13 0.2744579630041457 1105 14-15 0.444367585449541 1105 16-17 0.3401751980577217 1105 18-19 0.3730633231706335 1105 20-21 0.40088259920310065 1105 22-23 0.382939677175024 1105 24-25 0.2911930446685602 1105 26-27 0.39694989584650386 1105 28-29 0.4540082247423243 1105 30-31 0.5821119435969351 1105 32-33 0.6703545012247432 1105 34-35 0.6150258015514183 1105 36-37 0.558591691664077 1105 38-39 0.5244016792210999 1105 40-41 0.609340785792817 1105 42-43 0.5615766457533766 1105 44-45 0.6748152192909203 1105 46-47 0.7837163442658337 1105 48-49 1.0415653649029935 1105 50-51 1.2072573354616551 1105 52-53 1.2906113823684109 1105 54-55 1.3437770349415672 1105 56-57 1.4995681309882087 1105 58-59 1.5480248837359163 1105 60-61 1.6259732789694077 1105 62-63 1.7786387033857025 1105 64-65 1.9501770115853283 1105 66-67 1.9689032101460349 1105 68-69 2.1805945988257385 1105 70-71 2.1954901471432464 1105 72-73 2.250891258325307 1105 74-75 2.31934863113489 1105 76-77 2.2238330054012287 1105 78-79 2.5522233365897584 1105 80-81 2.7972084133582413 1105 82-83 3.083259296356303 1105 84-85 3.048525035167625 1105 86-87 2.8352646402719515 1105 88-89 2.7591819839837086 1105 90-91 2.802288001168673 1105 92-93 2.8472699401192045 1105 94-95 2.6880262008498654 1105 96-97 2.841467881108052 1105 98-99 2.4413043263064065 1105 100-101 2.283999245910925 1106 1 0.0036467554287362702 1106 2 0.06772080846952022 1106 3 0.1075550301357886 1106 4 0.17401983450007208 1106 5 0.12590073017296532 1106 6 0.12053610800395376 1106 7 0.07508595125665352 1106 8 0.06961730073030026 1106 9 -0.03168670910164195 1106 10-11 0.11574500214348404 1106 12-13 0.1539849400920943 1106 14-15 0.22647164173549328 1106 16-17 0.10061180480523291 1106 18-19 0.2578801564290458 1106 20-21 0.16440481508377758 1106 22-23 0.24402581495708375 1106 24-25 0.2646658975374834 1106 26-27 -0.0570142974504293 1106 28-29 0.1825293378681394 1106 30-31 0.21732147543961133 1106 32-33 0.028681647345656813 1106 34-35 0.028967990258877307 1106 36-37 0.10683763798769519 1106 38-39 0.21424555943666945 1106 40-41 0.42932152546354274 1106 42-43 0.538003366429777 1106 44-45 0.3974563761228609 1106 46-47 -0.03596724084915337 1106 48-49 0.33105745789806207 1106 50-51 0.5581884477961836 1106 52-53 0.4600332526069906 1106 54-55 0.2798088346880121 1106 56-57 0.6618098885269248 1106 58-59 0.9741293642878723 1106 60-61 1.0054354376415233 1106 62-63 1.0924595890578033 1106 64-65 1.4305997455082569 1106 66-67 1.2622073836102174 1106 68-69 1.3495985165518114 1106 70-71 0.9916084500119275 1106 72-73 1.3701137908902474 1106 74-75 1.385154475567326 1106 76-77 1.4450877215723281 1106 78-79 1.7842812623667825 1106 80-81 1.8484658906254978 1106 82-83 2.0857009303191347 1106 84-85 2.0333726782185018 1106 86-87 2.1356066626401997 1106 88-89 2.0626605442404227 1106 90-91 2.0323820131255665 1106 92-93 2.0700584115325995 1106 94-95 1.8441127342768056 1106 96-97 1.5561839343568025 1106 98-99 1.759756361520484 1106 100-101 1.5017394570674387 1107 1 0.020462810954082755 1107 2 0.021298140347361993 1107 3 0.0512129920634834 1107 4 0.08863765789364919 1107 5 0.06866171665605947 1107 6 0.004795557313535426 1107 7 0.01102634986337847 1107 8 -0.01286660076660695 1107 9 0.06918197754702504 1107 10-11 0.06109449723176397 1107 12-13 0.11193516689116478 1107 14-15 0.2846131460348005 1107 16-17 0.1624374151297232 1107 18-19 0.14737833888089114 1107 20-21 0.22347603778250402 1107 22-23 0.23143988389964676 1107 24-25 0.2466241803601008 1107 26-27 0.30106160923619996 1107 28-29 0.309423716775477 1107 30-31 0.2703661792771399 1107 32-33 0.27786523772166305 1107 34-35 0.3494144226451539 1107 36-37 0.40273865289363187 1107 38-39 0.2714459655889314 1107 40-41 0.4118275022745408 1107 42-43 0.33529194258738215 1107 44-45 0.32823825833590803 1107 46-47 0.3857023957221912 1107 48-49 0.41954190729839524 1107 50-51 0.44164915052562037 1107 52-53 0.4314038322033653 1107 54-55 0.6323349152543898 1107 56-57 0.7335594765521307 1107 58-59 0.9130249367422358 1107 60-61 0.9830206859141022 1107 62-63 1.26609142922862 1107 64-65 1.3960254890944768 1107 66-67 1.473779791059357 1107 68-69 1.7083287936340028 1107 70-71 1.5229150433120324 1107 72-73 1.862937568447041 1107 74-75 1.8958252579326533 1107 76-77 1.974455150025829 1107 78-79 2.3002637339250924 1107 80-81 2.238926883503588 1107 82-83 2.5359987306348657 1107 84-85 2.7396944142625337 1107 86-87 2.993774654523854 1107 88-89 2.9751916849547015 1107 90-91 2.998022854886198 1107 92-93 3.080143939760937 1107 94-95 2.92604663791003 1107 96-97 2.7050982645448585 1107 98-99 2.425630495088841 1107 100-101 1.8852167058102918 1108 1 0.09847603295047236 1108 2 0.09768704712302778 1108 3 0.032321075451505976 1108 4 0.04040964860976004 1108 5 0.03193920573747988 1108 6 0.0948203341531979 1108 7 0.08980780707558011 1108 8 0.09749776938693344 1108 9 0.2501185195829905 1108 10-11 0.1878141979304644 1108 12-13 0.19053734210708484 1108 14-15 0.2408288901255844 1108 16-17 0.16190580503640462 1108 18-19 0.11267991831121549 1108 20-21 0.11088064044275825 1108 22-23 0.14004944594049817 1108 24-25 0.17021487136103985 1108 26-27 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2316 94-95 1.3067778535428083 2316 96-97 1.139734690922456 2316 98-99 1.2491266212860506 2316 100-101 0.6700888739604078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 17.0 4 67.0 5 133.0 6 293.0 7 734.0 8 1654.0 9 2927.0 10 4871.0 11 7336.0 12 10751.0 13 14985.0 14 20262.0 15 26996.0 16 33934.0 17 41542.0 18 42455.0 19 41970.0 20 43979.0 21 48096.0 22 52439.0 23 58002.0 24 63056.0 25 70370.0 26 78940.0 27 90376.0 28 103364.0 29 117567.0 30 132993.0 31 149531.0 32 168407.0 33 188804.0 34 212887.0 35 230925.0 36 221619.0 37 144265.0 38 29842.0 39 302.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.049636993150266 19.85915366801403 15.418671157159656 22.672538181676053 2 16.52324007449851 20.815779028466167 39.080126606755755 23.580854290279575 3 19.915796003749815 24.292808141809203 29.76855095123324 26.022844903207737 4 13.855921181977266 17.213803737294427 35.780765800585094 33.14950928014322 5 13.975633881360693 37.34533050568041 33.90924373852623 14.769791874432672 6 32.99495216544816 36.14092417583446 16.576566015263584 14.287557643453791 7 29.2632750396162 32.26706427369757 20.036048401573332 18.433612285112897 8 26.587324362752103 35.87849865083077 18.840011609083252 18.694165377333878 9 26.41002616526614 16.654183756042677 19.58894318050452 37.34684689818667 10-11 25.416117657163255 26.72496852481197 26.763617979750858 21.095295838273913 12-13 26.313643888521867 23.730846742597887 27.431465907273058 22.524043461607192 14-15 22.74635414949422 25.26567175357808 25.739173369772505 26.248800727155203 16-17 22.462721287973242 28.43034773926129 26.436715638924944 22.670215333840517 18-19 22.43054393765287 26.7554362625916 28.98955386032547 21.824465939430056 20-21 22.941087158896682 25.920087011137312 29.293763129926077 21.845062700039932 22-23 22.696124104844838 25.834708146751513 29.181295047781635 22.287872700622014 24-25 22.562621710162105 26.05737229795299 29.21424489224377 22.165761099641138 26-27 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26.767261082665055 27.879791443552055 22.527503391749164 52-53 23.055651551656553 26.743367212155185 27.70659141115083 22.494389825037437 54-55 22.668790656723235 26.84616784243234 27.803265929823663 22.681775571020765 56-57 22.75460155538138 26.989821494026632 27.716325561582394 22.53925138900959 58-59 22.753029779415208 26.676916200899015 27.748151277915024 22.82190274177076 60-61 22.62419826295225 26.842484034457648 27.877247377271193 22.656070325318915 62-63 22.63864855942756 26.87372516838666 27.929024214219332 22.558602057966446 64-65 22.46237322907402 26.773592163455373 27.815418682416826 22.94861592505378 66-67 22.41612418373152 26.897574269365293 27.711171468026496 22.975130078876695 68-69 22.69894910633129 26.66561782184102 27.734075910042495 22.901357161785196 70-71 22.543941648446054 26.773484951635236 27.55137948516615 23.13119391475256 72-73 22.58938495126474 26.834342040911523 27.375455974020348 23.20081703380339 74-75 22.498175395663363 27.060541043523074 27.22918210914899 23.212101451664573 76-77 22.494389825037437 26.83297940283234 27.587061001010703 23.085569771119516 78-79 22.53305963250657 26.878630128176166 27.566382830279846 23.021927409037417 80-81 22.523652731537886 27.06050369337968 27.37873637446763 23.03710720061481 82-83 22.583883212855426 27.05995442212633 27.30919379022333 23.04696857479491 84-85 22.51881649684548 26.969216437086985 27.27505129643979 23.236915769627746 86-87 22.572535329374467 26.959133695568994 27.198591058521632 23.269739916534913 88-89 22.49027861392756 27.146530483816278 26.897313750942832 23.46587715131333 90-91 22.44186108043485 26.960151978764657 27.147133566359845 23.45085337444065 92-93 22.441047045742767 26.804226104013807 27.133776084709325 23.620950765534104 94-95 22.292761845022373 26.68620238600902 27.155531829023243 23.86550393994537 96-97 22.41766078600658 26.631489353387906 27.02679654837369 23.92405331223183 98-99 22.218857270554732 26.767912363632963 26.719493694175554 24.293736671636754 100-101 22.520120006683772 26.47946134168384 26.970120240737916 24.030298410894467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1130.0 1 820.0 2 719.0 3 939.0 4 761.0 5 652.5 6 1135.5 7 2537.5 8 3945.0 9 4262.0 10 3450.5 11 2503.5 12 2342.5 13 2519.5 14 2666.5 15 2818.5 16 3092.0 17 3358.0 18 3621.0 19 3946.5 20 4311.5 21 4974.5 22 5932.5 23 7135.5 24 8685.5 25 10467.0 26 13028.5 27 16192.0 28 19339.5 29 22714.5 30 26598.0 31 31346.0 32 37365.0 33 41273.0 34 43590.5 35 46997.0 36 51376.5 37 56225.0 38 59963.0 39 63205.5 40 66670.5 41 71039.5 42 74897.5 43 78557.5 44 82832.0 45 86485.5 46 88031.0 47 90073.0 48 92387.0 49 92107.5 50 91063.0 51 88499.0 52 86010.0 53 85679.5 54 85792.0 55 82063.0 56 75736.0 57 69791.0 58 63953.0 59 56800.5 60 47739.5 61 39802.0 62 33131.0 63 26255.0 64 19875.0 65 15193.0 66 11645.5 67 9138.5 68 7014.0 69 5243.0 70 4089.5 71 3003.5 72 2242.0 73 1783.5 74 1411.5 75 1073.0 76 801.5 77 563.0 78 349.0 79 199.0 80 128.0 81 74.0 82 41.5 83 25.5 84 12.0 85 7.0 86 4.0 87 3.5 88 2.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02218425796913498 2 0.011071776454320578 3 0.0017910226617283288 4 0.0 5 2.8493542345677956E-4 6 0.0 7 0.0 8 0.0 9 2.0352530246912828E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001668907480246852 26-27 0.008609120294444125 28-29 2.238778327160411E-4 30-31 0.0 32-33 2.238778327160411E-4 34-35 0.009524984155555203 36-37 9.972739820987286E-4 38-39 0.011417769468518097 40-41 0.011051423924073664 42-43 0.00482354966851834 44-45 7.937486796296003E-4 46-47 8.141012098765132E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.035253024691283E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.141012098765132E-5 66-67 5.90223377160472E-4 68-69 0.001058331572839467 70-71 6.105759074073848E-5 72-73 2.238778327160411E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6282024197530263E-4 82-83 9.158638611110772E-4 84-85 0.0058004711203701554 86-87 0.004111211109876391 88-89 0.0 90-91 0.0015467922987653748 92-93 5.495183166666463E-4 94-95 2.035253024691283E-5 96-97 0.0 98-99 0.0 100-101 2.035253024691283E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2456697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.181307749290156 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34082343796506 43.45108603151828 2 12.945041253167952 14.545386929429707 3 4.055100484699989 6.834625448556772 4 1.8068009035033035 4.060337504056584 5 0.9702043318892033 2.725367407478089 6 0.6016802930834366 2.0281911427442187 7 0.3975059374953815 1.563268238461869 8 0.2897434234025995 1.302253155081146 9 0.22384992195688108 1.1318563219602709 >10 1.2480729625103022 12.814944230608457 >50 0.083019988603467 3.1708958672724337 >100 0.03409337141632361 3.41978353099517 >500 0.002830070034573096 1.129408942625087 >1k 0.0010159225765112435 0.8304129788215983 >5k 2.1769769496669502E-4 0.9921822703903596 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9975 0.40603297842591085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8140 0.3313391924197408 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6222 0.2532668863925832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.0352530246912826E-5 0.0 0.0 0.0 0.0 14-15 4.070506049382565E-5 0.0 0.0 0.0 0.0 16-17 4.070506049382565E-5 0.0 0.0 0.0 0.0 18-19 4.070506049382565E-5 0.0 0.0 0.0 0.0 20-21 4.070506049382565E-5 0.0 0.0 1.0176265123456414E-4 0.0 22-23 4.070506049382565E-5 0.0 0.0 1.8317277222221543E-4 0.0 24-25 8.14101209876513E-5 0.0 0.0 3.6634554444443085E-4 0.0 26-27 8.14101209876513E-5 0.0 0.0 6.716334981481233E-4 0.0 28-29 8.14101209876513E-5 0.0 0.0 0.0020556055549381955 0.0 30-31 8.14101209876513E-5 0.0 0.0 0.008120659568518217 0.0 32-33 8.14101209876513E-5 0.0 0.0 0.019049968311110405 0.0 34-35 1.2211518148147696E-4 0.0 0.0 0.036471734202467784 0.0 36-37 1.2211518148147696E-4 0.0 0.0 0.06527056450184944 0.0 38-39 1.2211518148147696E-4 0.0 0.0 0.11132834045061316 0.0 40-41 1.2211518148147696E-4 0.0 0.0 0.1658527689820926 0.0 42-43 1.6282024197530263E-4 0.0 0.0 0.22019402474134986 0.0 44-45 2.8493542345677956E-4 0.0 0.0 0.2892908649296189 0.0 46-47 2.8493542345677956E-4 0.0 0.0 0.37304152689566517 0.0 48-49 2.8493542345677956E-4 0.0 0.0 0.46704986410615557 0.0 50-51 2.8493542345677956E-4 0.0 0.0 0.5684868748567691 0.0 52-53 2.8493542345677956E-4 0.0 0.0 0.662576622188247 0.0 54-55 2.8493542345677956E-4 4.070506049382565E-5 0.0 0.7705264426178726 0.0 56-57 2.8493542345677956E-4 4.070506049382565E-5 0.0 0.8983810376289791 0.0 58-59 2.8493542345677956E-4 4.070506049382565E-5 0.0 1.0406452240548998 0.0 60-61 2.8493542345677956E-4 4.070506049382565E-5 0.0 1.198906499254894 0.0 62-63 2.8493542345677956E-4 4.070506049382565E-5 0.0 1.3593658477215547 0.0 64-65 2.8493542345677956E-4 4.070506049382565E-5 0.0 1.5213312834264867 0.0 66-67 3.052879537036924E-4 4.070506049382565E-5 0.0 1.7103248792993193 0.0 68-69 3.256404839506052E-4 4.070506049382565E-5 0.0 1.9243113823153608 0.0 70-71 3.256404839506052E-4 4.070506049382565E-5 0.0 2.155048017724612 0.0 72-73 3.256404839506052E-4 4.070506049382565E-5 0.0 2.393538967157936 0.0 74-75 3.6634554444443085E-4 4.070506049382565E-5 0.0 2.6329254279221246 0.0 76-77 3.6634554444443085E-4 4.070506049382565E-5 0.0 2.89408909605051 0.0 78-79 3.6634554444443085E-4 4.070506049382565E-5 0.0 3.1909714547622277 2.0352530246912826E-5 80-81 3.6634554444443085E-4 4.070506049382565E-5 0.0 3.50674096154308 4.070506049382565E-5 82-83 3.6634554444443085E-4 4.070506049382565E-5 0.0 3.8361059585288704 4.070506049382565E-5 84-85 3.6634554444443085E-4 4.070506049382565E-5 0.0 4.161420801995525 4.070506049382565E-5 86-87 3.866980746913437E-4 4.070506049382565E-5 0.0 4.49695261564613 4.070506049382565E-5 88-89 4.0705060493825655E-4 4.070506049382565E-5 0.0 4.8583524952405615 4.070506049382565E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4060 0.0 60.039764 1 GTATCAA 7655 0.0 44.009727 1 ATCAACG 10205 0.0 32.720654 3 TATCAAC 10465 0.0 32.04584 2 TCAACGC 10530 0.0 31.755219 4 CAACGCA 10885 0.0 30.763199 5 AACGCAG 11305 0.0 29.66231 6 GTGGTAT 1840 0.0 27.373835 1 TGGTATC 1800 0.0 25.86186 2 ACGCAGA 13215 0.0 25.231365 7 CGCAGAG 13350 0.0 24.94064 8 GTACATG 9675 0.0 24.507399 1 TACATGG 9635 0.0 23.812311 2 GCAGAGT 14655 0.0 22.330795 9 ACATGGG 9790 0.0 22.22094 3 GGACCGA 645 0.0 22.091866 6 AGAGTAC 13785 0.0 20.43237 10-11 GAGTACT 9260 0.0 20.286463 12-13 AGTACTT 9645 0.0 20.116882 12-13 GTACTTT 9455 0.0 19.842957 14-15 >>END_MODULE