FastQCFastQC Report
Thu 26 May 2016
SRR938331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800424
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108850.6045798100891789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80410.446617019102167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.34469658258276936No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.11491737501832901No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG19360.10753022621338086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19150.10636383429681008No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18080.10042078976952096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.044.265651
GTATCAA99850.041.1522561
TATCAAC126300.032.3825452
ATCAACG127350.032.0035253
TCAACGC129950.031.326614
CAACGCA133100.030.6209495
AACGCAG137750.029.6003976
GTACATG112850.026.7187211
ACGCAGA152950.026.5388857
CGCAGAG154750.026.1380528
TACATGG114950.025.6506652
GAGTACT82150.024.6146612-13
GCAGAGT162450.024.3418629
ACATGGG116800.024.3078983
AGTACTT84100.023.22457312-13
ACTTGAC7900.022.8628738
GTACTTT88600.022.6545814-15
CATGGGG50800.021.8126344
TAAGGTG6400.021.5493375
AGAGTAC147550.020.7758410-11
Produced by FastQC (version 0.11.5)