##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938331_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1800424 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.331467476549967 31.0 31.0 34.0 30.0 34.0 2 31.372515029792982 31.0 31.0 34.0 28.0 34.0 3 31.41039388499598 31.0 31.0 34.0 28.0 34.0 4 35.07761393982751 37.0 35.0 37.0 32.0 37.0 5 35.06715695858309 37.0 35.0 37.0 32.0 37.0 6 35.10124504005723 37.0 35.0 37.0 32.0 37.0 7 35.020163583689175 37.0 35.0 37.0 32.0 37.0 8 35.001285252807115 37.0 35.0 37.0 32.0 37.0 9 36.50956330286643 39.0 37.0 39.0 32.0 39.0 10-11 36.35156135443651 39.0 35.0 39.0 32.0 39.0 12-13 36.20929264439932 39.0 35.0 39.0 32.0 39.0 14-15 37.17374796159127 39.0 36.5 41.0 31.5 41.0 16-17 37.02341865027349 39.0 36.0 41.0 31.0 41.0 18-19 37.207586101940436 39.0 36.5 41.0 31.0 41.0 20-21 37.21060872327851 39.0 37.0 41.0 31.0 41.0 22-23 37.119624599538774 39.0 36.0 41.0 31.0 41.0 24-25 36.97980892278708 39.0 36.0 41.0 31.0 41.0 26-27 36.780364236424305 39.0 36.0 41.0 30.0 41.0 28-29 36.57378595264227 39.0 36.0 41.0 30.0 41.0 30-31 36.304075873238745 39.0 35.0 40.0 30.0 41.0 32-33 36.03002903760448 38.0 35.0 40.0 29.5 41.0 34-35 35.76578183805593 38.0 35.0 40.0 28.5 41.0 36-37 35.4865103997725 38.0 34.5 40.0 27.0 41.0 38-39 35.36350715164872 38.0 34.0 40.0 27.0 41.0 40-41 35.24759362239117 38.0 34.0 40.0 26.5 41.0 42-43 34.939164330180006 38.0 34.0 40.0 25.5 41.0 44-45 34.544628376426886 38.0 33.0 40.0 24.0 41.0 46-47 34.08008919010189 38.0 33.0 40.0 23.0 41.0 48-49 33.66791627972077 38.0 33.0 40.0 20.5 41.0 50-51 32.959970817985095 37.0 32.0 39.5 19.0 40.5 52-53 32.99648027353557 37.0 32.0 39.5 19.0 40.0 54-55 33.10321874180748 37.0 32.0 40.0 18.0 41.0 56-57 32.79611191585982 37.0 32.0 40.0 15.0 41.0 58-59 32.43845838535812 36.5 31.0 39.5 12.5 41.0 60-61 31.984142346469497 36.0 31.0 39.0 8.0 41.0 62-63 31.433554262773658 35.0 30.0 39.0 7.0 40.0 64-65 30.934015820717786 35.0 29.5 38.5 3.5 40.0 66-67 30.421437950171736 35.0 29.0 38.0 2.0 40.0 68-69 29.866393693929872 34.5 28.5 37.5 2.0 40.0 70-71 29.25841051885556 34.0 27.5 37.0 2.0 39.0 72-73 28.642152626270256 34.0 26.0 36.0 2.0 39.0 74-75 27.984527533514328 33.5 26.0 36.0 2.0 38.5 76-77 27.26736590936357 33.0 25.0 35.0 2.0 37.5 78-79 26.509071196562587 32.0 23.5 35.0 2.0 37.0 80-81 25.8248048792951 32.0 21.0 35.0 2.0 36.0 82-83 25.119372436714908 31.0 19.0 34.5 2.0 36.0 84-85 24.43348927808116 31.0 15.0 34.0 2.0 35.0 86-87 23.688016822703986 30.5 7.0 34.0 2.0 35.0 88-89 22.908948114444154 30.0 2.0 34.0 2.0 35.0 90-91 22.025101031756964 29.0 2.0 34.0 2.0 35.0 92-93 21.09100856242752 29.0 2.0 34.0 2.0 35.0 94-95 20.099523223418483 27.0 2.0 33.5 2.0 35.0 96-97 18.85664737861748 25.5 2.0 33.0 2.0 35.0 98-99 17.34906555344741 21.5 2.0 32.5 2.0 34.0 100-101 13.772399723620659 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4798846274706534 1101 2 0.29369919649262144 1101 3 0.31271505455771376 1101 4 0.18221719203199882 1101 5 0.2259732704508508 1101 6 0.4449820673902565 1101 7 0.26686910796062335 1101 8 0.3371122692542059 1101 9 0.5967066708542319 1101 10-11 0.6750079002026368 1101 12-13 0.68296325055951 1101 14-15 0.7732533747086308 1101 16-17 0.5678203942526281 1101 18-19 0.7798663965318937 1101 20-21 0.7343214559493134 1101 22-23 0.8737651968086126 1101 24-25 0.7655649451898654 1101 26-27 0.7916876814945155 1101 28-29 0.813679003638299 1101 30-31 0.7313106317322635 1101 32-33 0.7200675887508083 1101 34-35 0.6983446199086671 1101 36-37 0.8100068884908467 1101 38-39 0.8645833840758996 1101 40-41 0.8830520568646563 1101 42-43 0.9355969415551115 1101 44-45 0.9262313771501098 1101 46-47 0.9408799638443668 1101 48-49 0.7210508016430381 1101 50-51 0.8872505418717296 1101 52-53 0.8145179861465763 1101 54-55 0.8159307724858067 1101 56-57 0.804931582665958 1101 58-59 0.8178870881352225 1101 60-61 0.8277049170710349 1101 62-63 0.8410169936838194 1101 64-65 0.8427005858375907 1101 66-67 0.8190067173169204 1101 68-69 0.9838047910965813 1101 70-71 1.0186079704522122 1101 72-73 1.1499372808600121 1101 74-75 0.9620631023195507 1101 76-77 0.9758799477197257 1101 78-79 1.2541848932109652 1101 80-81 1.1075728008456913 1101 82-83 0.9225322754585328 1101 84-85 0.9691071109105742 1101 86-87 0.9220445201151186 1101 88-89 1.238057847673371 1101 90-91 1.035859826198486 1101 92-93 1.0442838059479556 1101 94-95 1.061567630832183 1101 96-97 0.8137690732611027 1101 98-99 1.0690344255189714 1101 100-101 1.2046750677894398 1102 1 0.46149607776727564 1102 2 0.2514877556405821 1102 3 0.15725066636171903 1102 4 0.12328080688296694 1102 5 0.19163756599112247 1102 6 0.2610900887769958 1102 7 0.3139398815618293 1102 8 0.48560741624871895 1102 9 0.5102291284469587 1102 10-11 0.5938133080622023 1102 12-13 0.5600033662896635 1102 14-15 0.632779336675334 1102 16-17 0.6336346611967869 1102 18-19 0.49757215813137634 1102 20-21 0.6573721440615756 1102 22-23 0.6459315042620233 1102 24-25 0.5670389489633791 1102 26-27 0.500461517888958 1102 28-29 0.36785358837739324 1102 30-31 0.4365800941408011 1102 32-33 0.6840540811391236 1102 34-35 0.5935393311867116 1102 36-37 0.5728488265324998 1102 38-39 0.536405272740268 1102 40-41 0.8628684182849398 1102 42-43 0.8070466949618762 1102 44-45 0.759844755870958 1102 46-47 0.9378556897118244 1102 48-49 0.972701829698714 1102 50-51 0.9978764433128262 1102 52-53 0.7173018554087989 1102 54-55 0.656409532812738 1102 56-57 0.7089142354900275 1102 58-59 0.6695153997979588 1102 60-61 0.822688791827165 1102 62-63 0.9785971607912849 1102 64-65 1.1716351900954933 1102 66-67 0.672433512969075 1102 68-69 0.7416100788925988 1102 70-71 0.8871109200338587 1102 72-73 0.8079134354376585 1102 74-75 0.8879845128594042 1102 76-77 1.0230130679428413 1102 78-79 1.0943733713700503 1102 80-81 1.4148928806864056 1102 82-83 1.1636627740370606 1102 84-85 1.2458407713887745 1102 86-87 1.3697654887347106 1102 88-89 1.4052768889489968 1102 90-91 1.308568888773813 1102 92-93 1.151839136260051 1102 94-95 1.369770594395522 1102 96-97 1.595188144105073 1102 98-99 1.7318836476214017 1102 100-101 1.5625386589363437 1103 1 0.295001213512041 1103 2 0.2283222939748697 1103 3 0.32059467737137837 1103 4 0.3157707319306269 1103 5 0.1901683797146987 1103 6 0.5034965860968512 1103 7 0.2587777480649507 1103 8 0.3336953433222405 1103 9 0.46596758229677704 1103 10-11 0.4787238709751165 1103 12-13 0.5328722937151298 1103 14-15 0.6821486147088578 1103 16-17 0.6968414090829356 1103 18-19 0.647826851853722 1103 20-21 0.5492674509799471 1103 22-23 0.6653064355336156 1103 24-25 0.7993316037174267 1103 26-27 0.7960448101715016 1103 28-29 0.74402681408165 1103 30-31 0.8355879910915363 1103 32-33 0.7806149336128527 1103 34-35 0.8684145472062639 1103 36-37 0.7847028055091343 1103 38-39 0.8865026771083109 1103 40-41 0.884813829521498 1103 42-43 0.9073801972665265 1103 44-45 0.8122503025408747 1103 46-47 0.6735997401779201 1103 48-49 0.6135786150356992 1103 50-51 0.7929996091023028 1103 52-53 0.6742052279512265 1103 54-55 0.5846317369557212 1103 56-57 0.5695590860815258 1103 58-59 0.7267689631706986 1103 60-61 0.8545875675748 1103 62-63 0.8458585647909906 1103 64-65 0.7869512633211713 1103 66-67 0.7581213439643761 1103 68-69 0.9435580768506391 1103 70-71 0.897699789562072 1103 72-73 1.1493074756671398 1103 74-75 0.8978258258410783 1103 76-77 1.1178082148003767 1103 78-79 0.8753525646526796 1103 80-81 0.857205564651327 1103 82-83 0.9326199706508405 1103 84-85 0.9104754610522257 1103 86-87 1.0254768833322139 1103 88-89 1.1181850761310734 1103 90-91 1.0129833614208756 1103 92-93 1.104579168665591 1103 94-95 0.9604850987209765 1103 96-97 1.2282837553506383 1103 98-99 1.4002593981207951 1103 100-101 1.2699958074216067 1104 1 0.3884031351173469 1104 2 0.35776053300655875 1104 3 0.4115808550241411 1104 4 0.20303331488234733 1104 5 0.3495317084149363 1104 6 0.4761619212556525 1104 7 0.3944556226498648 1104 8 0.47083255514940703 1104 9 0.6266560563089456 1104 10-11 0.6556322161514601 1104 12-13 0.728842978562497 1104 14-15 0.7277291885760206 1104 16-17 0.7003169511576033 1104 18-19 0.7752710103690958 1104 20-21 0.8450598687888373 1104 22-23 0.8949793947606608 1104 24-25 0.7586094139827608 1104 26-27 0.7881027591056835 1104 28-29 0.744308501452629 1104 30-31 0.8006528283520069 1104 32-33 0.8937183417986745 1104 34-35 0.8079257993731446 1104 36-37 0.8739520074431155 1104 38-39 0.9629326454054663 1104 40-41 0.8786191656173372 1104 42-43 1.105248222973536 1104 44-45 1.0315680696102874 1104 46-47 0.9275465446458213 1104 48-49 0.7653812117652308 1104 50-51 0.8560118304009947 1104 52-53 0.7674986970266389 1104 54-55 1.081559638292262 1104 56-57 1.2845529230857693 1104 58-59 1.0792639786924312 1104 60-61 0.9399334906562196 1104 62-63 1.1107694862210344 1104 64-65 1.252035911243766 1104 66-67 1.2684778375523713 1104 68-69 1.4046440076943654 1104 70-71 1.4988348103589253 1104 72-73 1.5226908822565406 1104 74-75 1.521471542949449 1104 76-77 1.4793976256688843 1104 78-79 1.4938933488772612 1104 80-81 1.4397651253347092 1104 82-83 1.4513367082599231 1104 84-85 1.5296212657902437 1104 86-87 1.7556793801118964 1104 88-89 1.6056300328570714 1104 90-91 1.4799746048190094 1104 92-93 1.7042623715956005 1104 94-95 1.702398155075226 1104 96-97 1.630217362736282 1104 98-99 1.8841345313728013 1104 100-101 1.962040655290183 1105 1 0.256493171529133 1105 2 0.18615395591693584 1105 3 0.14682417446379858 1105 4 0.009924757324931477 1105 5 0.062334042191878325 1105 6 0.23486404493862523 1105 7 0.11257673405371094 1105 8 0.23262866717250574 1105 9 0.3370680926892078 1105 10-11 0.31819664609424336 1105 12-13 0.4161289157774988 1105 14-15 0.46563105240000624 1105 16-17 0.3509341162626356 1105 18-19 0.338953914845014 1105 20-21 0.3217558765530839 1105 22-23 0.37979318124845207 1105 24-25 0.48669422619297364 1105 26-27 0.5025759366587295 1105 28-29 0.4049020338010223 1105 30-31 0.5008906195956158 1105 32-33 0.6245379784914817 1105 34-35 0.5862203677797879 1105 36-37 0.678338310127053 1105 38-39 0.6990752818708401 1105 40-41 0.7090006561811251 1105 42-43 0.6937998468670585 1105 44-45 0.6628114971368717 1105 46-47 0.6304966422618321 1105 48-49 0.6664065176174603 1105 50-51 0.7026187452622779 1105 52-53 0.6883388139406676 1105 54-55 0.7309385888132169 1105 56-57 0.8690082212593211 1105 58-59 0.8535123241742824 1105 60-61 0.7465288199498055 1105 62-63 0.8987381162417272 1105 64-65 0.7886146109129797 1105 66-67 0.7991038747928236 1105 68-69 0.9643276958005274 1105 70-71 0.9765765028651252 1105 72-73 1.0846570623068459 1105 74-75 1.0062791369569304 1105 76-77 1.003788679674063 1105 78-79 1.0684206937443683 1105 80-81 1.0150169903971857 1105 82-83 1.2342320080547822 1105 84-85 1.1846708394098293 1105 86-87 1.3248468518010021 1105 88-89 1.358357820430225 1105 90-91 1.3326293211245996 1105 92-93 1.5007653653783528 1105 94-95 1.5382678720451004 1105 96-97 1.756577175115389 1105 98-99 1.8452541993657832 1105 100-101 1.503430067725132 1106 1 0.2486163691671024 1106 2 0.15363054796126363 1106 3 0.17726631350267397 1106 4 0.1617242544499149 1106 5 0.2682616982954755 1106 6 0.07358508412377773 1106 7 0.48200280940407225 1106 8 0.3878374007498593 1106 9 0.21750214258573664 1106 10-11 0.29685142172165513 1106 12-13 0.23116562026091003 1106 14-15 0.5524958019399335 1106 16-17 0.305709912200534 1106 18-19 0.25551272628037225 1106 20-21 0.29754069188469856 1106 22-23 0.2776172019566516 1106 24-25 0.19596370922936757 1106 26-27 0.1842985195218816 1106 28-29 0.47357885234133335 1106 30-31 0.1651760449833901 1106 32-33 0.441910422828073 1106 34-35 0.21346594740053604 1106 36-37 0.07844751354875967 1106 38-39 0.19296713677859145 1106 40-41 0.524705557527291 1106 42-43 0.021621609356351712 1106 44-45 0.3302382061367197 1106 46-47 0.4056083735405167 1106 48-49 0.8563876092472213 1106 50-51 0.4226307778615208 1106 52-53 0.45827939244916394 1106 54-55 0.6549444669233253 1106 56-57 -0.26780782490271804 1106 58-59 0.12753117861689844 1106 60-61 1.0274950680033506 1106 62-63 0.8111723076982464 1106 64-65 0.8666284905330564 1106 66-67 0.39515822708845505 1106 68-69 0.832023031735865 1106 70-71 0.6231896800780099 1106 72-73 0.9439118837727349 1106 74-75 1.0375959145122593 1106 76-77 0.9312610923281817 1106 78-79 1.041912742791535 1106 80-81 1.3537793179130766 1106 82-83 0.9898177293703476 1106 84-85 0.8301051926491922 1106 86-87 0.7100678202061594 1106 88-89 0.7723761355966943 1106 90-91 0.947103324643372 1106 92-93 1.0268183020740729 1106 94-95 1.0105364383120268 1106 96-97 1.1861190600203777 1106 98-99 1.2017898893359202 1106 100-101 0.9654178396382829 1107 1 0.2265129490242188 1107 2 0.12829183516092257 1107 3 0.22813956670666258 1107 4 0.0347755629368649 1107 5 0.21499272880651432 1107 6 0.2730123297561349 1107 7 0.12881062640069274 1107 8 0.1368920498902355 1107 9 0.3159281299567809 1107 10-11 0.4622737556667431 1107 12-13 0.3149286710674133 1107 14-15 0.3946970917543027 1107 16-17 0.4074997213320657 1107 18-19 0.3551236085581877 1107 20-21 0.3566613211367198 1107 22-23 0.4444099154045986 1107 24-25 0.35759967509248014 1107 26-27 0.24511567777100396 1107 28-29 0.4149591004521511 1107 30-31 0.4282552602828744 1107 32-33 0.5016108121112808 1107 34-35 0.45702142646381105 1107 36-37 0.5540443602278842 1107 38-39 0.619172124700718 1107 40-41 0.5818324687081642 1107 42-43 0.7568400729757272 1107 44-45 0.7957595847189154 1107 46-47 0.4657777837243344 1107 48-49 0.30369219398513536 1107 50-51 0.3299808773474595 1107 52-53 0.4379489016500244 1107 54-55 0.590093260046082 1107 56-57 0.48493665758560667 1107 58-59 0.4973538139710101 1107 60-61 0.3559888393064945 1107 62-63 0.7077953296325923 1107 64-65 0.6856064193830989 1107 66-67 0.6066448675087059 1107 68-69 0.6596787436409173 1107 70-71 0.8205976948880931 1107 72-73 0.8983174256387478 1107 74-75 0.8000528944975756 1107 76-77 1.064492264331566 1107 78-79 0.9589524472518285 1107 80-81 1.0021800959278728 1107 82-83 1.157493735634624 1107 84-85 0.9810422139403414 1107 86-87 1.2826344967469545 1107 88-89 1.2263205172475935 1107 90-91 1.2765697523452033 1107 92-93 1.406017218237242 1107 94-95 1.5611049175700238 1107 96-97 1.8442315080915854 1107 98-99 1.6593322584306875 1107 100-101 1.4794683322688211 1108 1 0.25943332252616713 1108 2 0.15244447099652447 1108 3 0.15022250178496677 1108 4 0.15143977880334347 1108 5 0.18600588960351416 1108 6 0.22150876384186802 1108 7 0.15739308357296267 1108 8 0.37566125803669337 1108 9 0.313924600804512 1108 10-11 0.2918543012148689 1108 12-13 0.3256815568846321 1108 14-15 0.29478715866115124 1108 16-17 0.4714104631892795 1108 18-19 0.4658139989706598 1108 20-21 0.3466069133725753 1108 22-23 0.26918382908741734 1108 24-25 0.43817279471604564 1108 26-27 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-0.8425404001490833 2316 56-57 -0.8637673494403657 2316 58-59 -0.8485622427946922 2316 60-61 -0.9029425185767934 2316 62-63 -0.9213802649730987 2316 64-65 -0.9680272246844837 2316 66-67 -0.8301451827329664 2316 68-69 -1.2793222005301956 2316 70-71 -1.4974524403997158 2316 72-73 -1.3017223557390984 2316 74-75 -1.3773447469703548 2316 76-77 -1.501446359736395 2316 78-79 -1.427008263120218 2316 80-81 -1.7453875387806157 2316 82-83 -1.595342809587553 2316 84-85 -1.5070375197111687 2316 86-87 -1.6072562515928261 2316 88-89 -2.01344278072483 2316 90-91 -1.9368717697367188 2316 92-93 -2.251241029961772 2316 94-95 -2.659943731999327 2316 96-97 -2.7726743891440044 2316 98-99 -2.401397438424464 2316 100-101 -2.3128326978877833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6225.0 3 1860.0 4 1973.0 5 2225.0 6 2686.0 7 3330.0 8 4056.0 9 5119.0 10 6506.0 11 8360.0 12 10201.0 13 12684.0 14 14807.0 15 18079.0 16 21840.0 17 26099.0 18 23868.0 19 20444.0 20 21825.0 21 23496.0 22 25952.0 23 28980.0 24 32466.0 25 36925.0 26 41606.0 27 47533.0 28 54486.0 29 62905.0 30 72872.0 31 85832.0 32 102333.0 33 123324.0 34 149576.0 35 184036.0 36 212713.0 37 206207.0 38 92586.0 39 4409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37765370079475 18.45398252306817 14.567109329695455 22.601254446441622 2 15.847456637661145 20.06079094952083 39.97524773118102 24.116504681637007 3 19.487589813849986 24.845727579760705 29.236583665194654 26.430098941194657 4 13.042402418165603 16.22786628914339 36.01742226927671 34.7123090234143 5 14.223922382503801 37.44175322446696 32.64322574077772 15.691098652251522 6 32.55775426190456 35.039818544183994 17.270046170053675 15.132381023857771 7 29.73940870415485 32.71461072421658 19.4911511185064 18.054829453122178 8 27.560671945652338 33.96384489271192 19.058016063627832 19.417467098007908 9 27.88855821116251 16.455213785279724 19.451868798288007 36.20435920526976 10-11 25.848289750505955 26.420123341124118 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36-37 22.976370649848114 25.60495976567091 28.86318883280296 22.55548075167802 38-39 22.48749438507821 25.910450713279104 29.058251566830556 22.543803334812125 40-41 22.628948834955825 25.828271205237463 29.09373970373424 22.44904025607247 42-43 22.765520417852166 25.68747982494301 28.80942578662582 22.737573970579003 44-45 22.438772129254872 26.092100581929657 28.58034770248461 22.88877958633086 46-47 22.717309187868015 26.279357849127877 28.14176202644151 22.861570936562604 48-49 22.997272631327522 26.289806529020648 27.925311203319502 22.787609636332327 50-51 22.713576659689068 26.539999650055314 27.714564003633868 23.031859686621754 52-53 23.084225451081302 26.598917099359397 27.589108780271708 22.727748669287585 54-55 22.50806421598934 26.40441230793501 27.778286969993392 23.309236506082254 56-57 22.797998985105156 26.79464044419842 27.651301684575458 22.756058886120968 58-59 23.03122267515952 26.201709680895863 27.7135684533432 23.05349919060142 60-61 22.86238011217678 26.31154389738758 28.080167065181644 22.745908925253996 62-63 22.774736201253614 26.327381521089453 27.93644833232976 22.961433945327165 64-65 22.554610543248916 26.25619316652692 27.96113139664289 23.228064893581273 66-67 22.62214838058513 26.54099516233588 27.70588957416446 23.13096688291453 68-69 22.435113530664374 26.306068550285367 27.992287697116026 23.266530221934236 70-71 22.43450332084296 26.513769088006434 27.805410186400003 23.246317404750602 72-73 22.71971568028032 26.463634593380743 27.55455651296109 23.26209321337785 74-75 22.422780072936412 26.686376758129786 27.504883539527224 23.385959629406575 76-77 22.533928431550653 26.577729704745284 27.488626635311793 23.399715228392274 78-79 22.77071921099085 26.53688148976801 27.3217080830557 23.370691216185442 80-81 22.463461007578026 27.007415833813337 27.329188924073662 23.19993423453498 82-83 22.742455596199463 26.808500356463515 27.154937901472763 23.29410614586426 84-85 22.689360591223302 26.491875123240956 27.05063224344358 23.768132042092166 86-87 22.840147905911486 26.237634349767543 27.358371868435622 23.563845875885352 88-89 22.73810605284001 26.30401910443515 27.250739877369128 23.70713496535571 90-91 22.580601250015974 26.487165084005763 27.35683683396701 23.575396832011254 92-93 22.855288020014143 26.09853066263243 27.286995528151976 23.75918578920145 94-95 22.692038407681537 26.044597808450582 27.245087906470182 24.018275877397702 96-97 22.9553254484722 26.006372751804683 27.00065316677534 24.03764863294778 98-99 22.877986571625637 25.989551634741105 26.754470638660283 24.37799115497297 100-101 23.2968864116642 25.50804659544028 27.126342164560295 24.068724828335224 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1510.0 1 1018.0 2 732.0 3 936.0 4 783.5 5 702.5 6 1220.5 7 2766.0 8 4287.5 9 4492.0 10 3629.0 11 2711.5 12 2501.5 13 2700.0 14 2909.5 15 2966.0 16 3073.5 17 3281.0 18 3453.0 19 3610.0 20 3872.0 21 4329.0 22 4920.5 23 5596.0 24 6346.5 25 7534.5 26 9028.5 27 10828.5 28 12429.5 29 13867.5 30 15921.0 31 18550.0 32 21612.5 33 24607.5 34 27634.0 35 30137.5 36 32819.5 37 35911.5 38 38274.5 39 39968.0 40 42749.0 41 48168.0 42 53297.5 43 57345.0 44 61050.0 45 64466.5 46 65712.5 47 66986.0 48 72259.5 49 74586.0 50 72108.5 51 68691.5 52 66123.5 53 65427.0 54 64826.0 55 61322.5 56 55891.0 57 51610.5 58 47322.5 59 41582.5 60 35759.0 61 30252.0 62 24783.5 63 19905.5 64 16138.0 65 13275.5 66 10573.5 67 8605.5 68 6549.0 69 4436.5 70 3345.5 71 2703.0 72 2323.5 73 2030.0 74 1606.0 75 1291.0 76 960.5 77 623.0 78 382.5 79 236.0 80 153.0 81 90.0 82 48.5 83 31.5 84 20.0 85 13.0 86 9.0 87 6.5 88 5.5 89 6.0 90 6.5 91 4.5 92 1.5 93 0.5 94 0.0 95 2.0 96 2.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005998586999506783 2 0.10003199246399737 3 0.11008517993539299 4 0.1130844734351464 5 0.14913153790440475 6 0.0910341119647372 7 0.026604844192256935 8 0.025382909803468516 9 0.023161210914762302 10-11 0.04787761105161895 12-13 0.05395951175945221 14-15 0.026743700372801073 16-17 0.031131555677995852 18-19 0.03551941098319063 20-21 0.0560423544676143 22-23 0.03124264062243116 24-25 0.04707224520446295 26-27 0.05445939400941112 28-29 0.05126570185689593 30-31 0.06440149653637144 32-33 0.04779429734329247 34-35 0.05940267403678244 36-37 0.05018262364865165 38-39 0.029576366455901497 40-41 0.034491875247164 42-43 0.030714987136363434 44-45 0.006692867902227475 46-47 0.012441513776754808 48-49 0.008553540721518932 50-51 0.0077481748743629284 52-53 0.009247821624239623 54-55 0.01038644230470156 56-57 0.013191337151693159 58-59 0.017884676054085037 60-61 0.0226613286648034 62-63 0.010441984776919214 64-65 0.008525769485410103 66-67 0.009719932638089695 68-69 0.010192043651939766 70-71 0.02802117723380715 72-73 0.02657707295614811 74-75 0.0277712361088277 76-77 0.021383851803797326 78-79 0.004998822499588986 80-81 0.004832195082936019 82-83 0.005526475985656712 84-85 0.0049432800273713304 86-87 0.012524827485081293 88-89 0.03513061367766704 90-91 0.033714280636116825 92-93 0.02760460869217473 94-95 0.04354529821864183 96-97 0.04057377595499727 98-99 0.023855491817482994 100-101 0.0709832794941636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1800424.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.22101819512348 #Duplication Level Percentage of deduplicated Percentage of total 1 76.59538782715008 37.70102977902693 2 12.552294256195621 12.356734079494972 3 4.327956071338852 6.390792136051942 4 2.0146070290543543 3.966440369324321 5 1.1034501487498718 2.7156469924514575 6 0.6856363840082104 2.0248632559504074 7 0.4560549097612602 1.5713240907934067 8 0.3200109100772916 1.2601010262041916 9 0.24219627702664806 1.0729032622487829 >10 1.4546749347259464 13.573297435432915 >50 0.14677074542355947 5.021609139451021 >100 0.09495538428850173 8.552320521027752 >500 0.004418863505480855 1.4475525497043145 >1k 0.0012463461169304976 0.9437868623303326 >5k 2.2660838489645411E-4 0.7945477413945665 >10k+ 1.1330419244822706E-4 0.6070507591127855 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10885 0.6045798100891789 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8041 0.446617019102167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6206 0.34469658258276936 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2069 0.11491737501832901 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1936 0.10753022621338086 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1915 0.10636383429681008 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1808 0.10042078976952096 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.33137083264831E-5 0.0 24-25 0.0 0.0 0.0 6.387384305030371E-4 0.0 26-27 0.0 0.0 0.0 0.0014718755137678682 0.0 28-29 0.0 0.0 0.0 0.004637796430174226 0.0 30-31 0.0 0.0 0.0 0.013718990637760883 0.0 32-33 0.0 0.0 0.0 0.030076248705860398 0.0 34-35 0.0 0.0 0.0 0.052182152648487246 5.55424722176554E-5 36-37 0.0 0.0 0.0 0.09417226164503473 5.55424722176554E-5 38-39 0.0 0.0 0.0 0.15660200041767938 5.55424722176554E-5 40-41 0.0 0.0 0.0 0.2265022017035987 5.55424722176554E-5 42-43 0.0 0.0 0.0 0.29512492612851193 5.55424722176554E-5 44-45 0.0 0.0 0.0 0.3835207706629105 5.55424722176554E-5 46-47 0.0 0.0 0.0 0.4899123761958294 5.55424722176554E-5 48-49 0.0 0.0 0.0 0.6041354703114377 5.55424722176554E-5 50-51 0.0 0.0 0.0 0.7322719537175688 5.55424722176554E-5 52-53 0.0 0.0 0.0 0.8590476465543672 5.55424722176554E-5 54-55 0.0 0.0 0.0 0.9940158540432698 5.55424722176554E-5 56-57 0.0 0.0 0.0 1.1623095448627656 5.55424722176554E-5 58-59 0.0 0.0 0.0 1.3460995854309874 5.55424722176554E-5 60-61 0.0 0.0 0.0 1.5437752440536228 5.55424722176554E-5 62-63 0.0 0.0 0.0 1.7442280262871412 5.55424722176554E-5 64-65 0.0 0.0 0.0 1.950235055742425 5.55424722176554E-5 66-67 0.0 2.77712361088277E-5 0.0 2.1830413280427274 5.55424722176554E-5 68-69 0.0 5.55424722176554E-5 0.0 2.4451184832017345 5.55424722176554E-5 70-71 0.0 5.55424722176554E-5 0.0 2.7416875136079057 5.55424722176554E-5 72-73 0.0 5.55424722176554E-5 0.0 3.033091094097835 5.55424722176554E-5 74-75 0.0 5.55424722176554E-5 0.0 3.319967963102025 5.55424722176554E-5 76-77 0.0 5.55424722176554E-5 0.0 3.6404757990340055 5.55424722176554E-5 78-79 0.0 5.55424722176554E-5 0.0 3.996391961004741 5.55424722176554E-5 80-81 0.0 5.55424722176554E-5 0.0 4.3860501748477025 5.55424722176554E-5 82-83 0.0 5.55424722176554E-5 0.0 4.783095537495612 5.55424722176554E-5 84-85 0.0 5.55424722176554E-5 0.0 5.1792522205880385 5.55424722176554E-5 86-87 0.0 5.55424722176554E-5 0.0 5.592293815234633 5.55424722176554E-5 88-89 0.0 1.110849444353108E-4 0.0 6.0322735089067905 5.55424722176554E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5705 0.0 44.26565 1 GTATCAA 9985 0.0 41.152256 1 TATCAAC 12630 0.0 32.382545 2 ATCAACG 12735 0.0 32.003525 3 TCAACGC 12995 0.0 31.32661 4 CAACGCA 13310 0.0 30.620949 5 AACGCAG 13775 0.0 29.600397 6 GTACATG 11285 0.0 26.718721 1 ACGCAGA 15295 0.0 26.538885 7 CGCAGAG 15475 0.0 26.138052 8 TACATGG 11495 0.0 25.650665 2 GAGTACT 8215 0.0 24.61466 12-13 GCAGAGT 16245 0.0 24.341862 9 ACATGGG 11680 0.0 24.307898 3 AGTACTT 8410 0.0 23.224573 12-13 ACTTGAC 790 0.0 22.862873 8 GTACTTT 8860 0.0 22.65458 14-15 CATGGGG 5080 0.0 21.812634 4 TAAGGTG 640 0.0 21.549337 5 AGAGTAC 14755 0.0 20.77584 10-11 >>END_MODULE