FastQCFastQC Report
Thu 26 May 2016
SRR938331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800424
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100860.5602013747872723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83120.46166902907315166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64980.3609149844703248No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.11536171479607026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20270.11258459118518749No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG19340.10741914126894554No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC19150.10636383429681008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54550.047.484351
GTATCAA93050.040.198241
TATCAAC123350.030.1697222
ATCAACG124550.029.9403863
TCAACGC126150.029.5606424
CAACGCA128550.029.0087535
AACGCAG133450.027.9327496
ACGCAGA151850.024.4855337
GTACATG113050.024.257941
CGCAGAG154150.024.1201938
TACATGG115600.023.5994972
AGTACTT79750.022.626412-13
GAGTACT78200.022.52836812-13
GCAGAGT163700.022.306959
ACATGGG118600.022.1098543
CGCGGAA4050.021.12527782-83
TGGTATC27900.020.9531632
GTACTTT83600.020.90278414-15
AGAGTAC148600.020.259710-11
GAGTGCA10350.020.1871459
Produced by FastQC (version 0.11.5)