##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938331_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1800424 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69425257605986 31.0 31.0 34.0 30.0 34.0 2 31.91583982439692 31.0 31.0 34.0 30.0 34.0 3 31.966231843165833 31.0 31.0 34.0 30.0 34.0 4 35.66844143379559 37.0 35.0 37.0 33.0 37.0 5 35.56761685025305 37.0 35.0 37.0 33.0 37.0 6 35.5726373343168 37.0 35.0 37.0 33.0 37.0 7 35.51067637400968 37.0 35.0 37.0 33.0 37.0 8 35.48895093600174 37.0 35.0 37.0 33.0 37.0 9 37.03479624799492 39.0 37.0 39.0 33.0 39.0 10-11 36.927856715973576 39.0 37.0 39.0 33.0 39.0 12-13 36.80477182041564 39.0 37.0 39.0 32.0 39.0 14-15 37.767830522143676 39.5 37.0 41.0 32.0 41.0 16-17 37.68455985923316 39.0 37.0 41.0 32.0 41.0 18-19 37.96008912345092 40.0 37.0 41.0 33.0 41.0 20-21 37.94244800113751 40.0 37.0 41.0 33.0 41.0 22-23 37.82469129493941 40.0 37.0 41.0 32.0 41.0 24-25 37.6832671081923 40.0 37.0 41.0 32.0 41.0 26-27 37.47287805539139 39.0 36.0 41.0 32.0 41.0 28-29 37.33462145583485 39.0 36.0 41.0 31.5 41.0 30-31 37.04180043145392 39.0 36.0 40.5 31.0 41.0 32-33 36.82197165778727 39.0 35.0 40.0 30.5 41.0 34-35 36.682314277081396 39.0 35.0 40.0 30.0 41.0 36-37 36.612063880508146 39.0 35.0 40.0 30.0 41.0 38-39 36.44392515318614 39.0 35.0 40.0 30.0 41.0 40-41 36.23818361674806 38.5 35.0 40.0 30.0 41.0 42-43 35.98058235171271 38.0 35.0 40.0 29.5 41.0 44-45 35.612704840637534 38.0 35.0 40.0 28.0 41.0 46-47 35.11784835127726 38.0 34.0 40.0 26.5 41.0 48-49 34.68577262911403 38.0 33.5 40.0 24.5 41.0 50-51 34.2755539806179 38.0 33.0 40.0 24.0 41.0 52-53 33.761685580729875 37.0 33.0 40.0 23.0 41.0 54-55 33.391046220223686 37.0 32.0 40.0 22.0 41.0 56-57 33.03374066331042 36.5 32.0 39.0 20.5 41.0 58-59 32.52267826911883 36.0 31.0 39.0 18.5 40.0 60-61 32.09689134337245 35.5 31.0 39.0 17.5 40.0 62-63 31.594052289905044 35.0 30.0 38.5 14.0 40.0 64-65 31.031157382927578 35.0 29.0 38.0 10.0 40.0 66-67 30.497867446779203 34.0 29.0 37.5 8.0 40.0 68-69 29.975008664625665 34.0 28.0 37.0 7.0 39.0 70-71 29.370535218370783 34.0 27.5 36.0 4.0 39.0 72-73 28.849908410463314 33.5 26.5 36.0 2.0 39.0 74-75 28.220800489218092 33.0 26.0 35.0 2.0 38.0 76-77 27.063879397297526 31.5 25.5 34.0 2.0 36.0 78-79 27.74450851577184 32.5 26.0 35.0 2.0 37.0 80-81 27.32772974588208 32.5 26.0 35.0 2.0 36.0 82-83 26.8474912020724 32.0 25.0 35.0 2.0 36.0 84-85 26.25632128876309 32.0 24.0 34.5 2.0 35.5 86-87 25.5778780442829 31.0 21.5 34.0 2.0 35.0 88-89 24.77979076039866 31.0 19.0 34.0 2.0 35.0 90-91 24.11258070321213 31.0 15.5 34.0 2.0 35.0 92-93 23.197856171657342 30.0 4.5 34.0 2.0 35.0 94-95 22.300707777723467 29.5 2.0 34.0 2.0 35.0 96-97 21.13868538744207 29.0 2.0 34.0 2.0 35.0 98-99 19.687322264088905 28.0 2.0 33.5 2.0 35.0 100-101 15.93988832630536 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.311703574363694 1101 2 0.30294097037461754 1101 3 0.288822367951834 1101 4 0.303843440573651 1101 5 0.190431109619837 1101 6 0.2902860371769478 1101 7 0.20452695400359033 1101 8 0.29688446793526424 1101 9 0.4990146728297944 1101 10-11 0.40470141103123325 1101 12-13 0.4681485965928829 1101 14-15 0.7119392146525527 1101 16-17 0.5770085023216822 1101 18-19 0.5345167849412249 1101 20-21 0.5773611705861015 1101 22-23 0.4978434006264294 1101 24-25 0.4953438316737149 1101 26-27 0.5320862347772177 1101 28-29 0.5607084580755171 1101 30-31 0.7133989811477974 1101 32-33 0.5807785171394002 1101 34-35 0.6075012759380556 1101 36-37 0.671006332181662 1101 38-39 0.7446205197424547 1101 40-41 0.7585381459684513 1101 42-43 0.8158155554821107 1101 44-45 1.0064760361732326 1101 46-47 0.9283586791473653 1101 48-49 1.0613053066889506 1101 50-51 1.0370908663574028 1101 52-53 0.9684471340045135 1101 54-55 0.7997750272181108 1101 56-57 0.7774820906704747 1101 58-59 0.9347117069709014 1101 60-61 0.9468589113331376 1101 62-63 1.185942177559614 1101 64-65 1.0089431922754777 1101 66-67 1.0497407861332384 1101 68-69 1.2330121173165658 1101 70-71 0.8984880706473781 1101 72-73 0.8254005445759276 1101 74-75 0.8468081215306249 1101 76-77 0.9899698332085478 1101 78-79 1.2360414537045976 1101 80-81 1.291605752575169 1101 82-83 1.3585638759015914 1101 84-85 1.555461879478294 1101 86-87 1.529001470365575 1101 88-89 1.7414145622126647 1101 90-91 1.87569389421445 1101 92-93 2.1860402197151423 1101 94-95 1.9875642940730707 1101 96-97 2.1102214987338606 1101 98-99 2.034724550371884 1101 100-101 2.035408767283867 1102 1 0.26296086189674384 1102 2 0.22220549011580815 1102 3 0.18234514036413074 1102 4 0.21498746713454864 1102 5 0.10646842006064361 1102 6 0.1624449062462645 1102 7 0.12647423036671768 1102 8 0.15250142016694923 1102 9 0.16121172457533106 1102 10-11 0.3918902604922252 1102 12-13 0.43616910060667635 1102 14-15 0.48385690723493724 1102 16-17 0.4917854541953446 1102 18-19 0.3769523649854847 1102 20-21 0.37173506698145076 1102 22-23 0.24416765059193324 1102 24-25 0.32493307546165795 1102 26-27 0.40458723865041435 1102 28-29 0.3831303095170995 1102 30-31 0.27678635953716224 1102 32-33 0.4489189612387108 1102 34-35 0.3548457573823782 1102 36-37 0.5416364744276336 1102 38-39 0.5174340592064794 1102 40-41 0.4384439531859101 1102 42-43 0.4857397302612938 1102 44-45 0.5178774431698301 1102 46-47 0.47865805095884895 1102 48-49 0.7425524922173707 1102 50-51 0.9530051811109246 1102 52-53 0.6281958462535613 1102 54-55 0.5871759786522617 1102 56-57 0.5861852161189773 1102 58-59 0.7633811837623767 1102 60-61 0.7461162149158227 1102 62-63 0.7625853119773787 1102 64-65 0.802791926407572 1102 66-67 0.6482834801431174 1102 68-69 0.9213084835270422 1102 70-71 1.0279293800992768 1102 72-73 1.0914997849656736 1102 74-75 1.1104749177260018 1102 76-77 1.0887762657749676 1102 78-79 1.2355916012222607 1102 80-81 1.2650471719052767 1102 82-83 1.3556204671895422 1102 84-85 1.3411836388505805 1102 86-87 1.2235988577951993 1102 88-89 1.1183535961671893 1102 90-91 1.382304333693316 1102 92-93 1.618537346986514 1102 94-95 1.5508458066638475 1102 96-97 1.6115908095660352 1102 98-99 1.573869895471237 1102 100-101 1.5631111924131371 1103 1 0.2608920807342763 1103 2 0.23982469908564852 1103 3 0.2055898725267049 1103 4 0.21875937194200645 1103 5 0.11826720769885668 1103 6 0.07655532289616929 1103 7 0.28966268047651766 1103 8 0.2054308673386771 1103 9 0.3138375299829548 1103 10-11 0.31059478984749944 1103 12-13 0.37455044474225474 1103 14-15 0.5617637131435131 1103 16-17 0.5094311860006044 1103 18-19 0.4186228685230091 1103 20-21 0.48080575749006016 1103 22-23 0.2821309173589057 1103 24-25 0.4169276791076868 1103 26-27 0.361919148690923 1103 28-29 0.3245149922518351 1103 30-31 0.3562031790081406 1103 32-33 0.3323250237628699 1103 34-35 0.44513857842565585 1103 36-37 0.4613992351150742 1103 38-39 0.47938243265634384 1103 40-41 0.6157866571263924 1103 42-43 0.6827818976954845 1103 44-45 0.5407305909982725 1103 46-47 0.596757084842217 1103 48-49 0.6798095959416202 1103 50-51 0.7286309337708872 1103 52-53 0.587864745904561 1103 54-55 0.5298829290357787 1103 56-57 0.6344672069051285 1103 58-59 0.7621971924690172 1103 60-61 0.8051752681578819 1103 62-63 0.8660570021286738 1103 64-65 0.6904963273207905 1103 66-67 0.7320342501248689 1103 68-69 0.7981362868491928 1103 70-71 0.7477314184238786 1103 72-73 0.6715750561765681 1103 74-75 0.6998944222615542 1103 76-77 0.6972460203552977 1103 78-79 0.9590576649563332 1103 80-81 0.9128671289382808 1103 82-83 0.9216302913669843 1103 84-85 0.9374201081068918 1103 86-87 1.0261858176792664 1103 88-89 1.1316078616500462 1103 90-91 1.3070533874390122 1103 92-93 1.5090045929238265 1103 94-95 1.2104037574201847 1103 96-97 1.5365372945304152 1103 98-99 1.9666839653288086 1103 100-101 1.9597771825984953 1104 1 0.2304013026697973 1104 2 0.28992340534819405 1104 3 0.2631625487360374 1104 4 0.16268073778449121 1104 5 0.16635101055934598 1104 6 0.16988494281487476 1104 7 0.20802366486687163 1104 8 0.304015373839178 1104 9 0.36661920723169317 1104 10-11 0.33170644852338427 1104 12-13 0.386427732006112 1104 14-15 0.4325358720198125 1104 16-17 0.42704052205710497 1104 18-19 0.37682902318962164 1104 20-21 0.2903970967891496 1104 22-23 0.2815992107556795 1104 24-25 0.2918059953691454 1104 26-27 0.2799588243860498 1104 28-29 0.41863862929273665 1104 30-31 0.3060155416710941 1104 32-33 0.3600809182301674 1104 34-35 0.4797067378040225 1104 36-37 0.5415287361119709 1104 38-39 0.43046947405397873 1104 40-41 0.38444714402330504 1104 42-43 0.5865174077144744 1104 44-45 0.5137862106613298 1104 46-47 0.6069292091293974 1104 48-49 0.5348914212063818 1104 50-51 0.7812386531616795 1104 52-53 0.7380861047482412 1104 54-55 0.9801499247040439 1104 56-57 0.920302091832653 1104 58-59 0.959665534452526 1104 60-61 0.8538444254730422 1104 62-63 1.0397129282352005 1104 64-65 1.0067735481827746 1104 66-67 1.029528215826108 1104 68-69 1.1043622718439892 1104 70-71 1.1249968471699923 1104 72-73 1.0793729087586321 1104 74-75 1.2844046824492956 1104 76-77 1.2846791485253632 1104 78-79 1.4626185230211846 1104 80-81 1.5347044362615172 1104 82-83 1.6835411266134237 1104 84-85 1.69847807265824 1104 86-87 1.7936549173844476 1104 88-89 1.9955149753840686 1104 90-91 1.7799331694002625 1104 92-93 2.2845724937513623 1104 94-95 2.2950343276144203 1104 96-97 1.9999034759660042 1104 98-99 1.8729844499814767 1104 100-101 2.0680357993688983 1105 1 0.23956448465891356 1105 2 0.2779246303333842 1105 3 0.21441759560748253 1105 4 0.21977202845501154 1105 5 0.045744575333813486 1105 6 0.14739047664046012 1105 7 0.16129746031686665 1105 8 0.14932834179365528 1105 9 0.15212407452121113 1105 10-11 0.19679031722478868 1105 12-13 0.30876333291314495 1105 14-15 0.4097475445074181 1105 16-17 0.25403472589554354 1105 18-19 0.3027604848176324 1105 20-21 0.19421380387582587 1105 22-23 0.3748990596591639 1105 24-25 0.4500193634704388 1105 26-27 0.2555652370934638 1105 28-29 0.20210082251163897 1105 30-31 0.43409433028683253 1105 32-33 0.4241232680399989 1105 34-35 0.42340498837204166 1105 36-37 0.4815520310969603 1105 38-39 0.500517403851525 1105 40-41 0.45965847648744784 1105 42-43 0.6968837323310453 1105 44-45 0.5950605442941495 1105 46-47 0.606554282594729 1105 48-49 0.9079312197143281 1105 50-51 0.8159196390946164 1105 52-53 0.8665671231072594 1105 54-55 0.8834211305713566 1105 56-57 0.811663469220413 1105 58-59 0.8927515626406972 1105 60-61 0.9872214439401503 1105 62-63 0.8941192720372051 1105 64-65 0.8806763532637625 1105 66-67 0.7818898026322962 1105 68-69 0.893611337905373 1105 70-71 0.888494483894327 1105 72-73 0.9389544772350824 1105 74-75 0.8061065111450283 1105 76-77 0.7123219865278294 1105 78-79 0.9756348351402053 1105 80-81 0.8603948415199447 1105 82-83 0.9845314168043089 1105 84-85 1.0444522286231788 1105 86-87 1.0480307491471414 1105 88-89 1.2747260308754491 1105 90-91 1.4918657411711074 1105 92-93 1.6900296451653851 1105 94-95 1.9186519971251244 1105 96-97 1.9462521081034012 1105 98-99 1.9563177833148124 1105 100-101 2.0032210783795446 1106 1 0.027455375610532684 1106 2 0.1614274986084432 1106 3 0.13193530745875037 1106 4 0.1287523246524458 1106 5 0.02328203817431529 1106 6 0.10238788137395005 1106 7 0.05320966615857259 1106 8 0.1163718276747332 1106 9 -0.004542389916899481 1106 10-11 0.1685035975742366 1106 12-13 0.12718062480310266 1106 14-15 0.30430388875434744 1106 16-17 0.2775766579871899 1106 18-19 0.20545231545795417 1106 20-21 0.284571209148595 1106 22-23 0.33069358196160437 1106 24-25 0.3433168349393583 1106 26-27 0.27435311281727337 1106 28-29 0.3141531390397816 1106 30-31 0.21981042161325348 1106 32-33 0.16513673646201 1106 34-35 0.35395885868960875 1106 36-37 0.41743462393672814 1106 38-39 0.24720843905951284 1106 40-41 0.2695071758207632 1106 42-43 0.3342609627435209 1106 44-45 0.2723989805281164 1106 46-47 -0.28395412000188713 1106 48-49 0.05719022057042622 1106 50-51 0.5356620336155657 1106 52-53 0.559880772860879 1106 54-55 0.7234600258641564 1106 56-57 0.5423472713869586 1106 58-59 0.40026519621602574 1106 60-61 0.5804269014148531 1106 62-63 0.30744242653307907 1106 64-65 0.5803863238058007 1106 66-67 0.4965469915205105 1106 68-69 0.4750639456426349 1106 70-71 0.7078293878694666 1106 72-73 1.0502863545227932 1106 74-75 0.9395217470446404 1106 76-77 0.7531628865797337 1106 78-79 1.0436956991954247 1106 80-81 0.7410825197338617 1106 82-83 1.2234096886938666 1106 84-85 1.0517619484018041 1106 86-87 1.2068661148774886 1106 88-89 1.379234379544446 1106 90-91 1.392210877911321 1106 92-93 1.4033740336599294 1106 94-95 1.539261109543979 1106 96-97 1.9267935076598484 1106 98-99 2.2470466150404285 1106 100-101 2.0559934633957564 1107 1 0.0853337407424064 1107 2 0.1485643832064092 1107 3 0.09866530091094106 1107 4 0.018555167627944513 1107 5 0.030439674981963094 1107 6 0.05113676363964004 1107 7 -0.07060688695566597 1107 8 0.11428667741979837 1107 9 0.11023851209262148 1107 10-11 0.1734514164132932 1107 12-13 0.08777059545102617 1107 14-15 0.3060238619011457 1107 16-17 0.24042636885187108 1107 18-19 0.20950994614253915 1107 20-21 0.208492874797507 1107 22-23 0.2536035635979417 1107 24-25 0.15537487824098406 1107 26-27 0.10834522675694558 1107 28-29 0.174613422167873 1107 30-31 0.20281699866259117 1107 32-33 0.23784297464685267 1107 34-35 0.2552742961381327 1107 36-37 0.31001449539658665 1107 38-39 0.24510246488338083 1107 40-41 0.20447848293967752 1107 42-43 0.1375914386487338 1107 44-45 0.015238309771682168 1107 46-47 0.1820769619759588 1107 48-49 0.09998075840128706 1107 50-51 0.3077538695903712 1107 52-53 0.18037597949761164 1107 54-55 0.16438159608158998 1107 56-57 0.19348025818817405 1107 58-59 0.0392945201584638 1107 60-61 0.208262195506542 1107 62-63 0.39126243283266504 1107 64-65 0.08304433875379402 1107 66-67 0.1994355589947041 1107 68-69 0.2739550649435678 1107 70-71 0.3935644226412762 1107 72-73 0.4803229439451222 1107 74-75 0.5098066182866141 1107 76-77 0.2875024979805403 1107 78-79 0.5277871887484373 1107 80-81 0.5034025583036268 1107 82-83 0.726962856439787 1107 84-85 0.810990255254282 1107 86-87 0.7919461752696186 1107 88-89 0.8823720342137733 1107 90-91 0.8840667940287013 1107 92-93 1.0111393428275868 1107 94-95 1.0111821893649662 1107 96-97 1.2579735647973749 1107 98-99 1.2391707598116355 1107 100-101 1.2567065516853209 1108 1 0.10729903741177083 1108 2 0.1269667065649891 1108 3 0.11121952486506004 1108 4 0.10746407138501723 1108 5 0.004099699980123717 1108 6 0.1494567401119582 1108 7 0.056589642711273314 1108 8 0.022032431408476327 1108 9 0.21280449988763905 1108 10-11 0.1927790393821951 1108 12-13 0.27014689131162584 1108 14-15 0.36391482580086176 1108 16-17 0.3212843939970824 1108 18-19 0.3429743972858361 1108 20-21 0.2831748834733432 1108 22-23 0.3235157024112638 1108 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-1.9325657634206657 2316 94-95 -1.9644858109870107 2316 96-97 -1.9626861836066318 2316 98-99 -1.7812211574951569 2316 100-101 -2.172377483504503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 46.0 5 98.0 6 194.0 7 511.0 8 1022.0 9 1946.0 10 3041.0 11 4611.0 12 6439.0 13 9057.0 14 11672.0 15 15498.0 16 19959.0 17 24920.0 18 23143.0 19 18969.0 20 18776.0 21 20250.0 22 21904.0 23 24394.0 24 27688.0 25 32376.0 26 38378.0 27 45695.0 28 54077.0 29 63475.0 30 74791.0 31 87330.0 32 104178.0 33 126562.0 34 156553.0 35 198104.0 36 238003.0 37 236106.0 38 88432.0 39 2210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.873591436645754 19.508158114569078 15.375502106179905 22.242748342605264 2 16.370777332556123 20.942563443159408 38.75743705695089 23.92922216733357 3 19.492519539841727 25.08270274113209 29.816476563298423 25.608301155727766 4 13.536985475080025 17.176130276051797 35.56564207411259 33.7212421747556 5 14.119633727355927 37.70692298416964 32.791392515071045 15.38205077340339 6 32.482904027051404 35.543238703772 16.869581831835166 15.104275437341427 7 29.4134048424149 32.98756292962102 19.426257370486063 18.172774857478018 8 27.52290571554256 34.49709623955246 18.82556553345212 19.154432511452857 9 27.30190221858851 16.51627616605866 19.528788774199853 36.65303284115297 10-11 25.83388690663977 26.640002577170712 26.116514776519306 21.409595739670213 12-13 26.695322879499493 24.05477820779994 27.05204440731739 22.197854505383177 14-15 23.20792213389735 25.066178855647337 25.935390774617535 25.790508235837777 16-17 22.5367468996192 28.599207742176286 26.116125979213784 22.747919378990726 18-19 22.39789071907506 26.308913900281265 29.409877895429076 21.883317485214594 20-21 23.374382923133663 25.4184014432156 29.254886626705712 21.952329006945032 22-23 22.98941804819309 25.551758919010187 29.244555726873227 22.214267305923492 24-25 22.741421687750822 25.77698471109383 29.41829947370614 22.06329412744921 26-27 22.481934469753774 26.23987025033465 29.19475780247613 22.083437477435446 28-29 22.14224101780526 26.2304191848249 29.648643679846348 21.978696117523494 30-31 22.305750003193737 25.81858243644881 29.50376555419104 22.371902006166412 32-33 22.245519300672928 25.871846875431327 29.362122597743358 22.520511226152394 34-35 22.841395676056106 25.9524122314269 28.995243111788678 22.21094898072831 36-37 22.95663965898602 25.58938158994491 29.061214353986447 22.392764397082626 38-39 22.3880883029281 25.923127084319255 29.217184677628993 22.471599935123653 40-41 22.55475224886838 25.82396021340748 29.236191513522925 22.385096024201214 42-43 22.785105770822295 25.779992468348734 28.84658537371098 22.588316387117988 44-45 22.50164278814615 26.10225113356548 28.634061233365905 22.762044844922467 46-47 22.74192079199122 26.325965439252087 28.165060008086982 22.767053760669707 48-49 22.962146694334223 26.370593815678973 27.959997200659398 22.707262289327403 50-51 22.68912743909314 26.58845498227351 27.713502401948887 23.00891517668446 52-53 23.144770342985872 26.61864649660302 27.497994916752944 22.73858824365816 54-55 22.577797230228924 26.47292839958198 27.725621201715285 23.22365316847381 56-57 22.791075879903843 26.823181650544537 27.636156816394358 22.749585653157254 58-59 23.054485516416875 26.235362809007366 27.651527356644696 23.058624317931063 60-61 22.865447250203285 26.384673832386145 27.994683474559327 22.75519544285124 62-63 22.75286601808111 26.43652238975513 27.830697117914944 22.979914474248815 64-65 22.525226236897343 26.341610285088628 27.83825544347598 23.29490803453805 66-67 22.636744553810896 26.607458624682245 27.5807586268763 23.175038194630563 68-69 22.422523709862663 26.456665356588836 27.736866006813333 23.383944926735172 70-71 22.412671240960808 26.618515032713137 27.630082588902 23.338731137424055 72-73 22.693188574799205 26.559862165762667 27.364450641751787 23.38249861768634 74-75 22.505895075025574 26.78587648633807 27.172705629022353 23.535522809614008 76-77 22.433713100005225 26.642126871187187 27.47137301711673 23.452787011690855 78-79 22.625289392202756 26.62419377151864 27.272593426387015 23.477923409891588 80-81 22.355366712482347 27.033121736743915 27.351988870900897 23.259522679872845 82-83 22.640797607710265 26.83088840759603 27.17776375138767 23.350550233306034 84-85 22.480653743886982 26.491503923690995 27.10378470292411 23.924057629497913 86-87 22.630094952845752 26.27973039479809 27.38734695283242 23.702827699523734 88-89 22.457195364575256 26.455074102424277 27.126445790801085 23.96128474219939 90-91 22.353409801448272 26.494665722597578 27.43677186148184 23.715152614472313 92-93 22.511687467534813 26.237538229060476 27.21539662389827 24.035377679506446 94-95 22.26417966501701 26.109128364218996 27.23572922245457 24.39096274830942 96-97 22.50645496636528 26.05306101811502 27.019180709610136 24.421303305909564 98-99 22.364228348889576 26.168857655382745 26.77027738939656 24.696636606331122 100-101 22.422450697661112 25.869302383327124 27.23996059312513 24.468286325886634 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1521.0 1 1022.5 2 694.5 3 891.5 4 779.5 5 739.0 6 1262.5 7 2806.0 8 4158.0 9 4372.0 10 3554.0 11 2645.5 12 2491.0 13 2562.5 14 2803.5 15 2959.0 16 3078.0 17 3352.0 18 3510.0 19 3586.5 20 3875.0 21 4320.0 22 4817.0 23 5493.0 24 6472.5 25 7618.5 26 9113.0 27 11020.5 28 12569.5 29 14203.5 30 16331.0 31 18584.0 32 21596.0 33 24804.5 34 27877.0 35 30179.0 36 33013.5 37 36464.0 38 38831.0 39 40278.0 40 42825.5 41 48462.5 42 54061.5 43 58195.5 44 61333.5 45 64405.5 46 65508.0 47 66881.0 48 72813.5 49 75763.5 50 72867.5 51 69165.0 52 66006.0 53 65175.5 54 65583.5 55 61845.5 56 55979.0 57 51494.5 58 47009.0 59 41497.0 60 35559.0 61 29226.5 62 23744.5 63 19194.5 64 15490.5 65 12715.5 66 9861.0 67 8082.0 68 6075.5 69 3877.5 70 2940.5 71 2414.0 72 2019.0 73 1752.0 74 1496.0 75 1306.5 76 929.5 77 529.0 78 327.0 79 217.0 80 131.0 81 76.5 82 44.5 83 20.5 84 10.0 85 6.5 86 4.5 87 4.0 88 5.0 89 3.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.443397777412432E-4 2 0.04560036969069508 3 0.0 4 5.55424722176554E-5 5 0.043378670801988865 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005498704749547884 26-27 0.0018884440554002835 28-29 5.55424722176554E-5 30-31 0.001388561805441385 32-33 4.165685416324155E-4 34-35 0.010691925901898663 36-37 0.02113391067881788 38-39 0.004221227888541811 40-41 0.0010275357360266248 42-43 0.0012219343887884186 44-45 0.007276063860512856 46-47 0.0 48-49 0.0 50-51 6.109671943942093E-4 52-53 0.0 54-55 0.0012497056248972464 56-57 0.0 58-59 0.021439394276014982 60-61 0.0 62-63 0.00819251465210417 64-65 0.014690983901569853 66-67 0.005859730818962644 68-69 0.015357493568181717 70-71 2.77712361088277E-5 72-73 2.77712361088277E-5 74-75 0.022466930012041607 76-77 0.04987714005145454 78-79 0.029493052747575015 80-81 0.02543845227568617 82-83 0.03565826716373477 84-85 0.014968696262658128 86-87 0.008997880499260173 88-89 0.08303599596539481 90-91 0.02302235473421816 92-93 0.02327229585919761 94-95 0.02510519744238024 96-97 0.03593597952482304 98-99 0.05176558410685483 100-101 0.01280253984616957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1800424.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.23248283755521 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97690374028821 35.413453191261915 2 13.306072490649356 12.569576810997225 3 4.692178190424786 6.648696775499687 4 2.179116923753868 4.117004108089228 5 1.1653013857398018 2.7520038851267246 6 0.7274263473186229 2.0614891479187403 7 0.508472937203498 1.6811507495877853 8 0.35759579614305104 1.3512109843286841 9 0.2583139542174446 1.0980728468348826 >10 1.5530444088006217 13.945976673537347 >50 0.16476207058330328 5.40719921411554 >100 0.10480631724259477 9.177426254371404 >500 0.004239132448234008 1.356745157309822 >1k 0.0014130441494113362 1.0353288145987676 >5k 2.3550735823522266E-4 0.8237023768040691 >10k+ 1.1775367911761133E-4 0.5609630096182202 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10086 0.5602013747872723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8312 0.46166902907315166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6498 0.3609149844703248 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2077 0.11536171479607026 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2027 0.11258459118518749 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1934 0.10741914126894554 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1915 0.10636383429681008 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.55424722176554E-5 0.0 18-19 0.0 0.0 0.0 8.33137083264831E-5 0.0 20-21 0.0 0.0 0.0 1.110849444353108E-4 0.0 22-23 0.0 0.0 0.0 1.943986527617939E-4 0.0 24-25 0.0 0.0 0.0 7.498233749383479E-4 0.0 26-27 0.0 0.0 0.0 0.0015274179859855234 0.0 28-29 0.0 0.0 0.0 0.004721110138500709 0.0 30-31 0.0 0.0 0.0 0.013774533109978539 0.0 32-33 0.0 0.0 0.0 0.030298418594731015 0.0 34-35 0.0 0.0 0.0 0.05243209377346669 0.0 36-37 0.0 0.0 0.0 0.09478322883942894 0.0 38-39 0.0 0.0 0.0 0.15732405255650891 0.0 40-41 0.0 0.0 0.0 0.227585279911843 0.0 42-43 0.0 0.0 0.0 0.29637463175340917 0.0 44-45 0.0 0.0 0.0 0.3847704762878078 0.0 46-47 0.0 0.0 0.0 0.49135648047348846 0.0 48-49 0.0 0.0 0.0 0.6051630060474644 0.0 50-51 0.0 0.0 0.0 0.7331606332730513 0.0 52-53 0.0 0.0 0.0 0.8591309602626938 0.0 54-55 0.0 0.0 0.0 0.9935159717933109 0.0 56-57 0.0 0.0 0.0 1.1610876104739773 0.0 58-59 0.0 0.0 0.0 1.343239148111778 0.0 60-61 0.0 0.0 0.0 1.5385820229012723 0.0 62-63 0.0 0.0 0.0 1.737312988496043 0.0 64-65 0.0 0.0 0.0 1.9410150053542945 0.0 66-67 0.0 0.0 0.0 2.1735157940573995 0.0 68-69 0.0 0.0 0.0 2.435370779327536 0.0 70-71 0.0 0.0 0.0 2.7315232411920745 0.0 72-73 0.0 0.0 0.0 3.0227601942653513 0.0 74-75 0.0 0.0 0.0 3.3113033374360707 0.0 76-77 0.0 0.0 0.0 3.634727153159478 0.0 78-79 0.0 0.0 0.0 3.9934204387410963 0.0 80-81 0.0 0.0 0.0 4.384328358208956 0.0 82-83 0.0 0.0 0.0 4.784150844467748 0.0 84-85 0.0 0.0 0.0 5.185722918601396 0.0 86-87 0.0 0.0 0.0 5.603957734400341 0.0 88-89 0.0 0.0 0.0 6.048658538210999 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5455 0.0 47.48435 1 GTATCAA 9305 0.0 40.19824 1 TATCAAC 12335 0.0 30.169722 2 ATCAACG 12455 0.0 29.940386 3 TCAACGC 12615 0.0 29.560642 4 CAACGCA 12855 0.0 29.008753 5 AACGCAG 13345 0.0 27.932749 6 ACGCAGA 15185 0.0 24.485533 7 GTACATG 11305 0.0 24.25794 1 CGCAGAG 15415 0.0 24.120193 8 TACATGG 11560 0.0 23.599497 2 AGTACTT 7975 0.0 22.6264 12-13 GAGTACT 7820 0.0 22.528368 12-13 GCAGAGT 16370 0.0 22.30695 9 ACATGGG 11860 0.0 22.109854 3 CGCGGAA 405 0.0 21.125277 82-83 TGGTATC 2790 0.0 20.953163 2 GTACTTT 8360 0.0 20.902784 14-15 AGAGTAC 14860 0.0 20.2597 10-11 GAGTGCA 1035 0.0 20.187145 9 >>END_MODULE