FastQCFastQC Report
Thu 26 May 2016
SRR938330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823711
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110590.6064009045292812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82070.4500164773914288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.34276264166855386No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG19630.10763766846830446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.10747316872026325No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18280.10023517980644961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57550.043.345911
GTATCAA95900.039.117381
TATCAAC123050.030.3186992
ATCAACG125800.029.6559313
TCAACGC127650.029.3378244
GTACATG115850.028.806081
CAACGCA131200.028.5424395
AACGCAG133700.028.075976
TACATGG118900.027.8195382
ACCGTCG1405.8006117E-727.147488
ACATGGG123050.026.108843
ACGCAGA150650.024.7861527
CGCAGAG152050.024.5586078
CATGGGG53850.023.1226774
GCAGAGT159200.022.9172179
GAGTACT76500.022.41639312-13
AGTACTT79250.022.20797212-13
CATGGGA68500.021.9239624
ATGGGGA29400.021.6598855
TACACCG1805.150385E-621.1210845
Produced by FastQC (version 0.11.5)