##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938330_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823711 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24650177577478 31.0 31.0 34.0 28.0 34.0 2 31.33957737821398 31.0 31.0 34.0 28.0 34.0 3 31.400995552475145 31.0 31.0 34.0 28.0 34.0 4 35.04883394353601 37.0 35.0 37.0 32.0 37.0 5 35.07401282330369 37.0 35.0 37.0 32.0 37.0 6 35.11855935507325 37.0 35.0 37.0 32.0 37.0 7 35.04843749914323 37.0 35.0 37.0 32.0 37.0 8 35.023585425541654 37.0 35.0 37.0 32.0 37.0 9 36.53408846028784 39.0 37.0 39.0 32.0 39.0 10-11 36.39838137731252 39.0 37.0 39.0 32.0 39.0 12-13 36.24765711233852 39.0 35.0 39.0 32.0 39.0 14-15 37.2429439203909 39.0 36.5 41.0 31.5 41.0 16-17 37.11529924423333 39.0 36.0 41.0 31.0 41.0 18-19 37.133480304719335 39.0 36.5 41.0 31.0 41.0 20-21 37.23828199753141 39.5 37.0 41.0 31.0 41.0 22-23 37.160138037221905 39.0 36.0 41.0 31.0 41.0 24-25 37.038063048366766 39.0 36.0 41.0 31.0 41.0 26-27 36.862888363342655 39.0 36.0 41.0 30.0 41.0 28-29 36.67879587281099 39.0 36.0 41.0 30.0 41.0 30-31 36.44831308250046 39.0 35.5 40.0 30.0 41.0 32-33 36.16236207381542 38.5 35.0 40.0 30.0 41.0 34-35 35.89779438737826 38.0 35.0 40.0 29.0 41.0 36-37 35.61006431391816 38.0 35.0 40.0 27.0 41.0 38-39 35.36614820001634 38.0 34.5 40.0 27.0 41.0 40-41 35.29029243120209 38.0 34.0 40.0 26.5 41.0 42-43 35.086906861887655 38.0 34.0 40.0 25.5 41.0 44-45 34.74532998923623 38.0 33.5 40.0 24.5 41.0 46-47 34.309827050448234 38.0 33.0 40.0 23.5 41.0 48-49 33.90597386318336 38.0 33.0 40.0 22.5 41.0 50-51 33.139601066177704 37.5 32.0 39.5 20.0 40.5 52-53 33.21029784872713 37.0 32.5 39.5 20.0 40.5 54-55 33.34185460305937 37.5 33.0 40.0 19.5 41.0 56-57 33.0555490974173 37.0 32.0 40.0 17.5 41.0 58-59 32.74067875886037 37.0 32.0 40.0 15.0 41.0 60-61 32.31388690422989 36.0 31.0 39.0 12.0 41.0 62-63 31.81004144845318 35.5 31.0 39.0 8.0 40.5 64-65 31.33083503910433 35.0 30.0 39.0 7.0 40.0 66-67 30.815762201357565 35.0 29.5 38.0 2.0 40.0 68-69 30.281038497876033 34.5 29.0 37.5 2.0 40.0 70-71 29.682767170894948 34.0 28.0 37.0 2.0 39.0 72-73 29.159150490401167 34.0 28.0 36.5 2.0 39.0 74-75 28.571312834105843 34.0 26.5 36.0 2.0 39.0 76-77 27.865550791764704 33.5 26.0 35.5 2.0 37.5 78-79 27.12562516758412 33.0 25.0 35.0 2.0 37.0 80-81 26.499368595133767 32.0 23.5 35.0 2.0 36.5 82-83 25.876292625311795 32.0 21.5 35.0 2.0 36.0 84-85 25.248440679471692 31.0 19.5 34.5 2.0 35.5 86-87 24.60318466028883 31.0 17.5 34.0 2.0 35.0 88-89 23.854897788081555 31.0 9.0 34.0 2.0 35.0 90-91 23.064507753695622 30.0 2.0 34.0 2.0 35.0 92-93 22.251714224457714 29.5 2.0 34.0 2.0 35.0 94-95 21.33138364576405 29.0 2.0 34.0 2.0 35.0 96-97 20.126760764178094 28.0 2.0 34.0 2.0 35.0 98-99 18.68700495857074 25.5 2.0 33.0 2.0 35.0 100-101 15.243527894496442 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6674055503420924 1101 2 0.6615665574121934 1101 3 0.6788486035110814 1101 4 0.601865890988833 1101 5 0.5652253393932511 1101 6 0.4799156793514143 1101 7 0.484103885403556 1101 8 0.5066883557961148 1101 9 0.6789978555482605 1101 10-11 0.5866386617222403 1101 12-13 0.6451162629673917 1101 14-15 0.7965160695183542 1101 16-17 0.8451111706377006 1101 18-19 0.7774084753748838 1101 20-21 1.0001385254321278 1101 22-23 1.0396522086460251 1101 24-25 0.9869768413766025 1101 26-27 1.099547481259087 1101 28-29 0.9233955411455241 1101 30-31 0.9953104364748668 1101 32-33 0.9748898084794178 1101 34-35 0.9682118118577634 1101 36-37 1.1122150831674205 1101 38-39 1.0761072191313446 1101 40-41 1.1252721837834443 1101 42-43 1.25921465967361 1101 44-45 1.2424389159149172 1101 46-47 1.1208565892987892 1101 48-49 1.1344936082635613 1101 50-51 1.115508032741097 1101 52-53 1.0678382005847382 1101 54-55 1.2442893199312621 1101 56-57 1.0023322640197847 1101 58-59 1.1111959873193769 1101 60-61 1.0943702644070115 1101 62-63 1.1645342460488983 1101 64-65 1.1339650037371172 1101 66-67 1.2475840261974973 1101 68-69 1.2947324359081627 1101 70-71 1.466065181327501 1101 72-73 1.3901654798218779 1101 74-75 1.4694430831984455 1101 76-77 1.4784968183599219 1101 78-79 1.3320287887870528 1101 80-81 1.270919145474661 1101 82-83 1.351648200022158 1101 84-85 1.5023472521219716 1101 86-87 1.7765639834145297 1101 88-89 1.9346713233593924 1101 90-91 1.6644030154140736 1101 92-93 1.752242715896088 1101 94-95 1.7137913970239076 1101 96-97 1.5080025414405256 1101 98-99 1.5832500710281856 1101 100-101 1.5298091448576692 1102 1 0.4777786285424881 1102 2 0.5236267656600582 1102 3 0.46127011985248245 1102 4 0.46419226970301253 1102 5 0.5158226356873783 1102 6 0.43775330071537866 1102 7 0.31903179960387007 1102 8 0.3212237051875846 1102 9 0.5080126341139675 1102 10-11 0.48269237931781817 1102 12-13 0.47982989773267093 1102 14-15 0.5257840763166897 1102 16-17 0.6167643822652096 1102 18-19 0.5983099385798738 1102 20-21 0.5292170383938881 1102 22-23 0.6093571257180557 1102 24-25 0.632095909926484 1102 26-27 0.6225858781803026 1102 28-29 0.5838310106800009 1102 30-31 0.5286049431071476 1102 32-33 0.6266851160650333 1102 34-35 0.5264744512920103 1102 36-37 0.5386384846794599 1102 38-39 0.717010904493435 1102 40-41 0.725678887372105 1102 42-43 0.6682905841886466 1102 44-45 0.8035311427605194 1102 46-47 0.6643943855050125 1102 48-49 0.6449322523081875 1102 50-51 0.7882990620638068 1102 52-53 0.8070662329745062 1102 54-55 0.7855384164168342 1102 56-57 0.6962521003127407 1102 58-59 0.9951517688349298 1102 60-61 0.9917175948881649 1102 62-63 0.7239598550664574 1102 64-65 0.7773063466037513 1102 66-67 0.900075894567653 1102 68-69 0.886749885946255 1102 70-71 0.8763156745975422 1102 72-73 0.935853396171094 1102 74-75 0.7433556913759851 1102 76-77 0.7552915154628117 1102 78-79 0.798711694294095 1102 80-81 0.9993306450155792 1102 82-83 0.8132860763237062 1102 84-85 0.544261164290603 1102 86-87 0.6655849400653757 1102 88-89 0.9174117558741877 1102 90-91 0.8156336264828568 1102 92-93 1.0168540093380365 1102 94-95 0.9645349649779575 1102 96-97 0.9621662777901321 1102 98-99 1.2915058730559643 1102 100-101 1.3097936141772522 1103 1 0.6033547205163039 1103 2 0.5307455524169384 1103 3 0.5670414433273798 1103 4 0.5073839208836546 1103 5 0.5127236375552684 1103 6 0.4881588072816925 1103 7 0.4309885631089969 1103 8 0.4798256821367275 1103 9 0.5528658935927311 1103 10-11 0.4969413132337621 1103 12-13 0.5309561919216534 1103 14-15 0.5702806789585182 1103 16-17 0.6626834268434223 1103 18-19 0.6758363126504037 1103 20-21 0.702634474578332 1103 22-23 0.7170088947120519 1103 24-25 0.8450914925237782 1103 26-27 0.8567701056370751 1103 28-29 0.8105765663710827 1103 30-31 0.8862268163059284 1103 32-33 0.906501089508005 1103 34-35 0.8162253954012257 1103 36-37 0.8918480374622817 1103 38-39 0.9022264173019892 1103 40-41 0.8284959083637418 1103 42-43 0.7988045646583188 1103 44-45 0.8309500713430609 1103 46-47 0.652931141787306 1103 48-49 0.7031235208226505 1103 50-51 0.9125962119182773 1103 52-53 0.8854553996949903 1103 54-55 0.8071614471808033 1103 56-57 0.8386735760170225 1103 58-59 0.8402939412786665 1103 60-61 0.8861833450693339 1103 62-63 1.0587392480144011 1103 64-65 1.0847926722670138 1103 66-67 1.0234480685181282 1103 68-69 0.9529059922222203 1103 70-71 1.0847282317881977 1103 72-73 1.2455131461465818 1103 74-75 1.0742001997575699 1103 76-77 0.99758886382536 1103 78-79 0.894991700703752 1103 80-81 0.9937661406519886 1103 82-83 1.1888011808752807 1103 84-85 1.2179518480433167 1103 86-87 1.3019468370372884 1103 88-89 1.1846137964415853 1103 90-91 1.3400713463664324 1103 92-93 1.3168520362155505 1103 94-95 1.360351407186993 1103 96-97 1.3464232118208734 1103 98-99 1.0738597005548343 1103 100-101 0.8336567368767689 1104 1 0.5460169852111356 1104 2 0.45604110612235615 1104 3 0.5090509496072642 1104 4 0.4780149705502552 1104 5 0.3436603588265612 1104 6 0.4932099894102038 1104 7 0.3473146580831923 1104 8 0.3231187836108873 1104 9 0.5470419881929303 1104 10-11 0.47235297680972366 1104 12-13 0.5024692853521202 1104 14-15 0.6243203876136576 1104 16-17 0.6071493358231805 1104 18-19 0.6395312647478946 1104 20-21 0.6537856193652658 1104 22-23 0.6883375817326609 1104 24-25 0.7480869603263045 1104 26-27 0.598009794269295 1104 28-29 0.6568236226291333 1104 30-31 0.5280671347392669 1104 32-33 0.6018789615443438 1104 34-35 0.5232958133334691 1104 36-37 0.5748332809218937 1104 38-39 0.6121946913442997 1104 40-41 0.3959534128991038 1104 42-43 0.42277256482402237 1104 44-45 0.6621061528132941 1104 46-47 0.6090783769282098 1104 48-49 0.3643942622284797 1104 50-51 0.36143220758918204 1104 52-53 0.3450009198850452 1104 54-55 0.49584962428460244 1104 56-57 0.5760451891674734 1104 58-59 0.5355973027687355 1104 60-61 0.6385819229823397 1104 62-63 0.6633977969632205 1104 64-65 0.5877075018219813 1104 66-67 0.6384689055793871 1104 68-69 0.8021901443756327 1104 70-71 0.6504839327080418 1104 72-73 0.5569190422035071 1104 74-75 0.5484690326577457 1104 76-77 0.5886135650783473 1104 78-79 0.6450541284587317 1104 80-81 0.36997952619832475 1104 82-83 0.5295673967004006 1104 84-85 0.54391664454322 1104 86-87 0.5169314832617893 1104 88-89 0.6880468914370681 1104 90-91 0.7349340887342777 1104 92-93 0.7076562552961221 1104 94-95 0.5887981528597734 1104 96-97 0.5176084588477892 1104 98-99 0.6555367804778953 1104 100-101 0.5872161258097357 1105 1 0.3895229371198141 1105 2 0.44267014929142334 1105 3 0.4637913416682906 1105 4 0.48857854065047235 1105 5 0.3970186761969998 1105 6 0.46078501823740936 1105 7 0.4535859260074062 1105 8 0.43315894403140476 1105 9 0.45239061973358474 1105 10-11 0.4615573238773649 1105 12-13 0.5488935924832745 1105 14-15 0.607877339690134 1105 16-17 0.5666917276392667 1105 18-19 0.4316900995289714 1105 20-21 0.6056174426627265 1105 22-23 0.6306855246272889 1105 24-25 0.8025676794817542 1105 26-27 0.7158605994267049 1105 28-29 0.5768475192819125 1105 30-31 0.5857906551109906 1105 32-33 0.5224561801608374 1105 34-35 0.5297194901503204 1105 36-37 0.7909305959211892 1105 38-39 0.6177359281370656 1105 40-41 0.7740716371931313 1105 42-43 0.7386849876277992 1105 44-45 0.6657210606312063 1105 46-47 0.6632986767532429 1105 48-49 0.5952399643281154 1105 50-51 0.3835975694407381 1105 52-53 0.48388127710738615 1105 54-55 0.5859082683956061 1105 56-57 0.8363705253420122 1105 58-59 0.8634409118378557 1105 60-61 0.7890279947557914 1105 62-63 0.8410373694221356 1105 64-65 0.8172679709416997 1105 66-67 0.7953618762755781 1105 68-69 0.6042229356478792 1105 70-71 0.5534220267205257 1105 72-73 0.6358545740436377 1105 74-75 0.5895302773681479 1105 76-77 0.7078532733312919 1105 78-79 0.723312625330518 1105 80-81 0.5675908008624795 1105 82-83 0.678252182323039 1105 84-85 0.7302396258856341 1105 86-87 0.8749339730032872 1105 88-89 0.7168160396529863 1105 90-91 0.9101571799117352 1105 92-93 0.8325719714921291 1105 94-95 0.7263824954101636 1105 96-97 0.9800548579423456 1105 98-99 1.0495599356820087 1105 100-101 0.9465191917082691 1106 1 0.27855358652529105 1106 2 0.3258440615940046 1106 3 0.24145548504662528 1106 4 0.3822307600209882 1106 5 0.538757266017619 1106 6 0.21351549619267018 1106 7 0.3228692926981367 1106 8 0.3343906480838186 1106 9 0.417254319836978 1106 10-11 0.17307516124098044 1106 12-13 0.299167890837424 1106 14-15 0.6793423677493706 1106 16-17 0.29340357749307344 1106 18-19 0.05997405274543155 1106 20-21 0.33783161305863274 1106 22-23 0.49269558736410346 1106 24-25 0.5957670592046753 1106 26-27 0.3058726487652308 1106 28-29 0.3065766510314276 1106 30-31 0.5035403147116781 1106 32-33 0.5073035323022026 1106 34-35 0.32290870049537546 1106 36-37 0.1802472247229545 1106 38-39 0.2454978074820673 1106 40-41 0.3686166180004946 1106 42-43 0.1499379264405789 1106 44-45 0.4688280254457098 1106 46-47 0.12106538792696853 1106 48-49 0.6039596561464933 1106 50-51 0.4685137985035013 1106 52-53 0.5565370843443489 1106 54-55 0.2603834597860484 1106 56-57 -0.0011107231497575754 1106 58-59 -0.34991840746246794 1106 60-61 0.2955884802913573 1106 62-63 0.31044389552418394 1106 64-65 0.5701012420677571 1106 66-67 0.0963317600753193 1106 68-69 -0.20140493548356275 1106 70-71 -0.24721955108318383 1106 72-73 0.26129000206725905 1106 74-75 0.2687236478132995 1106 76-77 0.36385469044783747 1106 78-79 0.6859266941425766 1106 80-81 0.5908532527159736 1106 82-83 0.566927178351488 1106 84-85 0.4151678383233879 1106 86-87 0.46015871900246097 1106 88-89 0.3663512441152008 1106 90-91 0.5279944335860378 1106 92-93 0.5748281276761844 1106 94-95 0.4435791057641296 1106 96-97 0.5040747910223438 1106 98-99 0.40900484156579253 1106 100-101 -0.02357789012004119 1107 1 0.5379705500695202 1107 2 0.45923514420872635 1107 3 0.36784889425629785 1107 4 0.47185507890110046 1107 5 0.4696814641229494 1107 6 0.4434998410057531 1107 7 0.37123341239743013 1107 8 0.46604762602562033 1107 9 0.4090375150129262 1107 10-11 0.5015394676316376 1107 12-13 0.5150026679027917 1107 14-15 0.5545638062485594 1107 16-17 0.5785540255740145 1107 18-19 0.550545434543352 1107 20-21 0.523064240029349 1107 22-23 0.6806613671203436 1107 24-25 0.5372598636731212 1107 26-27 0.49821028040865656 1107 28-29 0.49979055032217445 1107 30-31 0.6028759450064243 1107 32-33 0.7463776971240037 1107 34-35 0.7923989745478792 1107 36-37 0.859018021511261 1107 38-39 0.9270372631065555 1107 40-41 0.8674657882972667 1107 42-43 0.7302593890079834 1107 44-45 0.8604928309809736 1107 46-47 0.792400241967286 1107 48-49 0.7369159929194851 1107 50-51 0.554628710177461 1107 52-53 0.578537283828723 1107 54-55 0.6642162701956593 1107 56-57 0.5613137926127578 1107 58-59 0.6027132684881735 1107 60-61 0.5069820195995405 1107 62-63 0.5684213733044423 1107 64-65 0.5396159095901822 1107 66-67 0.6113079954148439 1107 68-69 0.6726824777027858 1107 70-71 0.6824852445645853 1107 72-73 0.6668737215401848 1107 74-75 0.7163823083624479 1107 76-77 0.6170079529931272 1107 78-79 0.7891796446682839 1107 80-81 0.8360409631774104 1107 82-83 0.8242221810510308 1107 84-85 0.7070086020883544 1107 86-87 0.5939197692443372 1107 88-89 0.9343624065670362 1107 90-91 1.125668597540514 1107 92-93 1.0528834910955212 1107 94-95 1.0870753188134437 1107 96-97 1.0398629928224743 1107 98-99 0.9577793136368946 1107 100-101 0.5699092702415456 1108 1 0.4609404188773034 1108 2 0.3760792837882576 1108 3 0.29226609440124207 1108 4 0.4165720016585368 1108 5 0.3661411179077305 1108 6 0.396354029260543 1108 7 0.3666657364131751 1108 8 0.3348548809063914 1108 9 0.35452949111818555 1108 10-11 0.33346705656411046 1108 12-13 0.35682212020549287 1108 14-15 0.43059394379800864 1108 16-17 0.3423545311863947 1108 18-19 0.4785886446945895 1108 20-21 0.41767393736122216 1108 22-23 0.534968290365164 1108 24-25 0.5192585477247178 1108 26-27 0.5218803990559238 1108 28-29 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5753.0 3 1836.0 4 1895.0 5 2148.0 6 2562.0 7 3086.0 8 3864.0 9 4951.0 10 6100.0 11 7922.0 12 10003.0 13 12527.0 14 14116.0 15 17221.0 16 20736.0 17 25420.0 18 22993.0 19 18882.0 20 19938.0 21 21675.0 22 23788.0 23 26863.0 24 30301.0 25 33998.0 26 38861.0 27 44690.0 28 52025.0 29 59893.0 30 69978.0 31 83185.0 32 99409.0 33 121367.0 34 149699.0 35 188549.0 36 224581.0 37 230645.0 38 115623.0 39 6628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38143953313745 18.567003553433054 14.547268239976754 22.50428867345274 2 15.955292134979397 19.99060814458191 39.95894354792699 24.095156172511707 3 19.415979109979812 24.835096111647502 29.247728868603527 26.501195909769155 4 13.051168445284194 16.276489989927384 35.95358477564841 34.71875678914001 5 14.223990835302084 37.4187307902906 32.686654457310965 15.670623917096355 6 32.544607904189775 34.97596569302256 17.318088470171507 15.161337932616153 7 29.649774286795637 32.86126454245767 19.459878997975984 18.029082172770707 8 27.62962020345186 33.926140371470225 19.074626439965467 19.36961298511245 9 27.851729224159826 16.494948281527094 19.51138837646726 36.14193411784582 10-11 25.878714823088643 26.356302119974163 26.242479844543453 21.522503212393737 12-13 26.891634863671083 23.92647577098552 26.93222567945049 22.24966368589291 14-15 23.49070355169714 24.84295659829114 25.986092694345064 25.680247155666656 16-17 22.754795974811998 28.41897037000674 26.01379081868142 22.812442836499844 18-19 22.579541895292078 26.190200616225816 29.318805529027724 21.911451959454382 20-21 23.539286408841537 25.325883828651886 29.208923057595783 21.925906704910798 22-23 23.098245563990123 25.469383769574343 29.122090797082922 22.310279869352613 24-25 22.826835425535826 25.627454993275943 29.34733048715516 22.198379094033065 26-27 22.576276709637618 26.116641764781555 29.11271296323472 22.1943685623461 28-29 22.2354120256265 26.115701028603528 29.55003875571393 22.098848190056046 30-31 22.307542285685916 25.73604098508374 29.476311155938127 22.480105573292217 32-33 22.259349084607372 25.904215255099217 29.230875993414813 22.6055596668786 34-35 22.87761117392356 25.939539861768722 28.891368472099476 22.291480492208237 36-37 22.978855994312447 25.60125797617898 28.867020529609505 22.552865499899063 38-39 22.487174768951135 25.843632413671873 29.075121698121748 22.594071119255247 40-41 22.62653602406632 25.816749346649885 29.12570373965815 22.43101088962565 42-43 22.753663924008112 25.744116949410493 28.75088607386668 22.751333052714713 44-45 22.427684933576327 26.15282832168398 28.574695203452226 22.844791541287467 46-47 22.730611664054052 26.29774028386222 28.11243933797471 22.859208714109016 48-49 22.940652921508704 26.33319934390881 27.94746115631959 22.778686578262892 50-51 22.70872508100917 26.54964824280432 27.729401680998535 23.01222499518798 52-53 23.043836617049 26.628477294762288 27.56708914070623 22.760596947482483 54-55 22.482708698638497 26.386752086952107 27.846227034615506 23.28431217979389 56-57 22.778258064498438 26.768851208087902 27.699419296339283 22.753471431074377 58-59 23.007184981184803 26.14700859762899 27.767714855724567 23.07809156546164 60-61 22.85412123610075 26.25410297476124 28.12375155913727 22.768024230000748 62-63 22.7224621870351 26.390332120196508 27.986172762601534 22.90103293016686 64-65 22.49707857922712 26.243296909024405 28.0239241192828 23.23570039246567 66-67 22.625335078193825 26.551901785520894 27.703635829739564 23.11912730654572 68-69 22.46854098268061 26.33363914490478 27.955327016395927 23.242492856018686 70-71 22.395923740983914 26.577182459537816 27.79311137235351 23.23378242712475 72-73 22.63771128344064 26.5234893439656 27.56301484057081 23.275784532022946 74-75 22.434936266224113 26.68158778873797 27.53117037391286 23.35230557112505 76-77 22.560321884893355 26.555750478884395 27.512477317084805 23.371450319137445 78-79 22.753163802106396 26.615775676786463 27.3268548858284 23.30420563527874 80-81 22.455785453891405 26.992533483073117 27.410221933661184 23.141459129374294 82-83 22.758559046646624 26.85887895873386 27.17042858568588 23.21213340893363 84-85 22.628109868778207 26.496657765007154 27.212839227255532 23.66239313895911 86-87 22.85297209498464 26.224172355571106 27.418417299168286 23.504438250275967 88-89 22.73930437434388 26.26120132381027 27.34768448384234 23.651809818003514 90-91 22.580013482537154 26.489784701763313 27.44107697936316 23.48912483633637 92-93 22.86084957788748 26.108809764995783 27.35216738175063 23.678173275366103 94-95 22.650366557736 26.05063795197178 27.343572596861087 23.95542289343114 96-97 22.933257931582236 26.026850629387045 27.094140110129704 23.945751328901014 98-99 22.817224292581532 26.037167748463684 26.86802624831698 24.277581710637808 100-101 23.244452496239447 25.532626239335947 27.1744512884702 24.04846997595441 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1436.0 1 994.5 2 808.5 3 998.0 4 791.0 5 720.5 6 1246.5 7 2867.5 8 4602.5 9 4852.5 10 3760.0 11 2818.0 12 2618.5 13 2767.0 14 2968.0 15 3029.0 16 3192.0 17 3389.5 18 3446.0 19 3610.0 20 3929.5 21 4264.0 22 4899.0 23 5618.0 24 6370.5 25 7594.0 26 9219.5 27 11009.5 28 12512.0 29 14087.0 30 16321.5 31 18745.5 32 21661.5 33 24732.5 34 27820.5 35 30166.5 36 32838.0 37 36276.0 38 38901.5 39 40568.5 40 43197.0 41 48683.0 42 54211.0 43 58576.5 44 62053.5 45 65569.5 46 66657.5 47 67583.0 48 73225.5 49 76020.5 50 73646.0 51 70022.5 52 67138.5 53 66608.5 54 65957.5 55 62125.0 56 56632.0 57 51920.5 58 47289.0 59 41871.0 60 36075.5 61 30317.5 62 25072.5 63 20235.5 64 16327.5 65 13242.5 66 10456.0 67 8537.0 68 6407.5 69 4336.0 70 3314.0 71 2706.5 72 2291.5 73 2018.5 74 1659.0 75 1329.0 76 937.0 77 606.5 78 407.5 79 268.0 80 167.0 81 93.0 82 51.5 83 33.0 84 25.5 85 22.5 86 14.5 87 10.5 88 7.5 89 4.0 90 5.0 91 3.5 92 0.5 93 1.0 94 2.5 95 2.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17645339639888116 2 0.04688242819174749 3 0.04556643020741773 4 0.051927087131678214 5 0.05839741055463283 6 0.060755240276556974 7 0.032954782857590924 8 0.03163878487326117 9 0.03662861056384482 10-11 0.03802685842219518 12-13 0.03501102970810616 14-15 0.031172702253811045 16-17 0.02969220452144008 18-19 0.03717694305731555 20-21 0.04718401106315639 22-23 0.043263433734840664 24-25 0.06305823674913405 26-27 0.04882900854356858 28-29 0.041344270007693106 30-31 0.04943217428638639 32-33 0.0456212634567648 34-35 0.045182597461988216 36-37 0.04293443423875822 38-39 0.024647545581509354 40-41 0.0228106317283824 42-43 0.019657719890925702 44-45 0.004194743575051091 46-47 0.010171567753882057 48-49 0.010089317879861448 50-51 0.008334653900755109 52-53 0.009157152640961204 54-55 0.009787735008452545 56-57 0.008087904278693278 58-59 0.01014415112920852 60-61 0.01022640100322913 62-63 0.0035093279582126776 64-65 0.006141323926872185 66-67 0.003810910829621579 68-69 0.008115320903366818 70-71 0.020425385381784723 72-73 0.01814980553388119 74-75 0.018917471024740215 76-77 0.011076316368108763 78-79 0.0040850770763569445 80-81 0.005647824682748527 82-83 0.004660826194501212 84-85 0.004441493197112919 86-87 0.007237988913813647 88-89 0.026813458930718737 90-91 0.03355794860040873 92-93 0.020946301250581917 94-95 0.029226121901989952 96-97 0.03224195061607897 98-99 0.019520636767558018 100-101 0.11904298433249566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1823711.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.748763596136435 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0964981368091 37.096101981651444 2 12.770191978782105 12.450621397018532 3 4.442732948987521 6.497332147528763 4 2.0734039149016645 4.043035091473801 5 1.1417916759738749 2.7830466244043417 6 0.6822639869708347 1.9955715486599235 7 0.4681666488361451 1.5975781701396063 8 0.3438173546510081 1.3408536753704796 9 0.2575777679869946 1.130093794729663 >10 1.4743983510117051 13.673513771986427 >50 0.1464810516193629 4.94886472066377 >100 0.09680291395284739 8.674055009069058 >500 0.004066109665555629 1.310054601030822 >1k 0.0014683173792284215 1.0542985542192318 >5k 2.2589498141975718E-4 0.7960647846721147 >10k+ 1.1294749070987859E-4 0.6089141273819973 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11059 0.6064009045292812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8207 0.4500164773914288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6251 0.34276264166855386 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1963 0.10763766846830446 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1960 0.10747316872026325 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1828 0.10023517980644961 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6449974804121927E-4 0.0 22-23 0.0 0.0 0.0 3.838327454295116E-4 0.0 24-25 0.0 0.0 0.0 8.224987402060962E-4 0.0 26-27 0.0 0.0 0.0 0.0017546639791063386 0.0 28-29 0.0 0.0 0.0 0.0042221601997246274 0.0 30-31 0.0 0.0 0.0 0.01337931284068583 0.0 32-33 0.0 0.0 0.0 0.030295370264257878 0.0 34-35 0.0 0.0 0.0 0.0563411637041176 0.0 36-37 0.0 0.0 0.0 0.09960459743895825 0.0 38-39 0.0 0.0 0.0 0.16507549715936354 0.0 40-41 0.0 0.0 0.0 0.23794888554162363 0.0 42-43 0.0 0.0 0.0 0.3118366890368046 0.0 44-45 0.0 0.0 0.0 0.40445004718401106 0.0 46-47 0.0 0.0 0.0 0.5136230466340336 0.0 48-49 0.0 0.0 0.0 0.6346400279430238 0.0 50-51 0.0 0.0 0.0 0.7658559936305698 0.0 52-53 0.0 0.0 0.0 0.8938916308559854 0.0 54-55 0.0 0.0 0.0 1.0347582484286162 0.0 56-57 0.0 0.0 0.0 1.2028769909267423 0.0 58-59 0.0 0.0 0.0 1.386568376239437 0.0 60-61 0.0 0.0 0.0 1.5856679046186595 0.0 62-63 0.0 0.0 0.0 1.7861656808562323 0.0 64-65 0.0 0.0 0.0 1.9907211175454882 0.0 66-67 0.0 0.0 0.0 2.2306988333129536 0.0 68-69 0.0 0.0 0.0 2.5003961702265327 0.0 70-71 0.0 0.0 0.0 2.7919993902542672 0.0 72-73 0.0 0.0 0.0 3.0825881951690812 0.0 74-75 0.0 0.0 0.0 3.3810729879898735 0.0 76-77 0.0 0.0 0.0 3.7079614039724493 0.0 78-79 0.0 0.0 0.0 4.057824951431449 0.0 80-81 0.0 0.0 0.0 4.444755775449071 0.0 82-83 0.0 0.0 0.0 4.843091915330883 2.741662467353654E-5 84-85 5.483324934707308E-5 0.0 0.0 5.241044222467266 5.483324934707308E-5 86-87 5.483324934707308E-5 0.0 0.0 5.6615055784606225 5.483324934707308E-5 88-89 5.483324934707308E-5 0.0 0.0 6.108944893132739 5.483324934707308E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5755 0.0 43.34591 1 GTATCAA 9590 0.0 39.11738 1 TATCAAC 12305 0.0 30.318699 2 ATCAACG 12580 0.0 29.655931 3 TCAACGC 12765 0.0 29.337824 4 GTACATG 11585 0.0 28.80608 1 CAACGCA 13120 0.0 28.542439 5 AACGCAG 13370 0.0 28.07597 6 TACATGG 11890 0.0 27.819538 2 ACCGTCG 140 5.8006117E-7 27.14748 8 ACATGGG 12305 0.0 26.10884 3 ACGCAGA 15065 0.0 24.786152 7 CGCAGAG 15205 0.0 24.558607 8 CATGGGG 5385 0.0 23.122677 4 GCAGAGT 15920 0.0 22.917217 9 GAGTACT 7650 0.0 22.416393 12-13 AGTACTT 7925 0.0 22.207972 12-13 CATGGGA 6850 0.0 21.923962 4 ATGGGGA 2940 0.0 21.659885 5 TACACCG 180 5.150385E-6 21.121084 5 >>END_MODULE