FastQCFastQC Report
Thu 26 May 2016
SRR938330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823711
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104030.5704302929576013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87140.47781693481039483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66670.36557327339693624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.11476599088342397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19720.10813116771242813No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG19390.10632167048397471No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC18970.10401867401139764No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59000.047.164391
GTATCAA97750.038.0540051
TATCAAC127750.029.005942
ATCAACG129500.028.5256213
TCAACGC132150.027.9528314
CAACGCA134550.027.454235
AACGCAG138750.026.6318666
GTACATG113600.025.24931
TACATGG116950.024.3633482
ACGCAGA155800.023.6259487
ACATGGG116850.023.4763723
GAGTACT84300.023.18447712-13
CGCAGAG158500.023.1635558
GTAGCGC3700.023.1288833
TAGCGCG3200.022.2850284
AGTACTT87600.021.76889812-13
GTACTTT89250.021.73896214-15
GCAGAGT167500.021.6637469
CATGGGG47300.021.2076844
CATGGGA66000.020.5292424
Produced by FastQC (version 0.11.5)