##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938330_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823711 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.695565251292557 31.0 31.0 34.0 30.0 34.0 2 31.923554225422777 31.0 31.0 34.0 30.0 34.0 3 31.976182629813604 31.0 31.0 34.0 30.0 34.0 4 35.67060076952982 37.0 35.0 37.0 33.0 37.0 5 35.53992326635086 37.0 35.0 37.0 33.0 37.0 6 35.58054209246969 37.0 35.0 37.0 33.0 37.0 7 35.51194898753147 37.0 35.0 37.0 33.0 37.0 8 35.506094989831176 37.0 35.0 37.0 33.0 37.0 9 37.0493301844426 39.0 37.0 39.0 33.0 39.0 10-11 36.95798731268276 39.0 37.0 39.0 33.0 39.0 12-13 36.83374229798471 39.0 37.0 39.0 32.0 39.0 14-15 37.814153667987966 40.0 37.0 41.0 32.0 41.0 16-17 37.74514629785092 39.0 37.0 41.0 32.0 41.0 18-19 37.882354166860864 39.5 37.0 41.0 32.5 41.0 20-21 37.95468114191338 40.0 37.0 41.0 33.0 41.0 22-23 37.846589454140485 40.0 37.0 41.0 32.0 41.0 24-25 37.7310686835798 40.0 37.0 41.0 32.0 41.0 26-27 37.52343216660974 39.0 36.5 41.0 32.0 41.0 28-29 37.38184613680566 39.0 36.0 41.0 31.5 41.0 30-31 37.11200184678384 39.0 36.0 40.5 31.0 41.0 32-33 36.87608864562422 39.0 35.0 40.0 30.5 41.0 34-35 36.687475702016386 39.0 35.0 40.0 30.0 41.0 36-37 36.6594035458469 39.0 35.0 40.0 30.0 41.0 38-39 36.52534310534948 39.0 35.0 40.0 30.0 41.0 40-41 36.320212742040816 39.0 35.0 40.0 30.0 41.0 42-43 36.06967222328538 38.0 35.0 40.0 30.0 41.0 44-45 35.735662613210096 38.0 35.0 40.0 28.5 41.0 46-47 35.260606532504326 38.0 34.0 40.0 26.5 41.0 48-49 34.827598506561614 38.0 34.0 40.0 25.5 41.0 50-51 34.43205776573152 38.0 33.0 40.0 24.0 41.0 52-53 33.92301548874794 37.5 33.0 40.0 23.0 41.0 54-55 33.5763928604916 37.0 33.0 40.0 22.5 41.0 56-57 33.22751850485082 37.0 32.0 39.5 22.0 41.0 58-59 32.765477370043826 36.0 31.0 39.0 20.0 40.5 60-61 32.37577280610799 36.0 31.0 39.0 18.5 40.0 62-63 31.903249199023314 35.0 30.5 39.0 17.0 40.0 64-65 31.378555045179855 35.0 30.0 38.0 13.5 40.0 66-67 30.872509953605586 34.5 29.0 38.0 10.0 40.0 68-69 30.31737649221834 34.0 29.0 37.0 8.0 39.0 70-71 29.72666392865975 34.0 28.0 36.5 7.0 39.0 72-73 29.28110265277777 34.0 28.0 36.0 4.0 39.0 74-75 28.737721053390587 33.0 26.5 35.5 2.0 38.0 76-77 27.52030118807201 31.5 26.0 34.5 2.0 36.0 78-79 28.22131933184589 33.0 26.5 35.0 2.0 37.0 80-81 27.919382785978698 33.0 26.0 35.0 2.0 36.5 82-83 27.436199869387202 33.0 26.0 35.0 2.0 36.0 84-85 26.898399746451055 32.0 25.0 35.0 2.0 35.5 86-87 26.279993924475974 32.0 24.5 34.0 2.0 35.0 88-89 25.60524940629299 31.0 22.0 34.0 2.0 35.0 90-91 25.029921955836205 31.0 20.0 34.0 2.0 35.0 92-93 24.239633637127813 31.0 16.5 34.0 2.0 35.0 94-95 23.408797501358492 30.5 4.5 34.0 2.0 35.0 96-97 22.342168797578125 30.0 2.0 34.0 2.0 35.0 98-99 21.036577615641953 29.0 2.0 34.0 2.0 35.0 100-101 17.48419733170442 22.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4103509267163865 1101 2 0.387405628529951 1101 3 0.45703169187679293 1101 4 0.34659793395694294 1101 5 0.18635515884885478 1101 6 0.21108568659983007 1101 7 0.40779235398258606 1101 8 0.3645585433791396 1101 9 0.29301645462767567 1101 10-11 0.3764589391846016 1101 12-13 0.5561516510343552 1101 14-15 0.5834330321730548 1101 16-17 0.5989519252286541 1101 18-19 0.6047860658847384 1101 20-21 0.6163252813565165 1101 22-23 0.6342986277179179 1101 24-25 0.7151017433053042 1101 26-27 0.7238869032890207 1101 28-29 0.6188822928071716 1101 30-31 0.5655259146445459 1101 32-33 0.4732539910576037 1101 34-35 0.5266231231211691 1101 36-37 0.7233531631014429 1101 38-39 0.8368604159275037 1101 40-41 0.9351114642838994 1101 42-43 1.0367322229735336 1101 44-45 0.8263723366619473 1101 46-47 0.9582076103112414 1101 48-49 1.0435664338089552 1101 50-51 1.0533156103563144 1101 52-53 1.0766304948391152 1101 54-55 0.9948107864278768 1101 56-57 1.0594340583722968 1101 58-59 1.1961045028663477 1101 60-61 1.1637088268688132 1101 62-63 1.2256238677650515 1101 64-65 1.113299981915457 1101 66-67 1.0474124610724829 1101 68-69 1.0434444438995527 1101 70-71 0.9852809832815197 1101 72-73 1.0864933590269459 1101 74-75 1.1730774682514138 1101 76-77 1.1229333662442649 1101 78-79 0.960985190045843 1101 80-81 1.1455470994118535 1101 82-83 1.2029565746561879 1101 84-85 1.2151613023779824 1101 86-87 1.3418770523134818 1101 88-89 1.2583195728403247 1101 90-91 1.4362255902112615 1101 92-93 1.5000370439927373 1101 94-95 1.519200163141896 1101 96-97 1.6797685049586981 1101 98-99 1.7462433278045566 1101 100-101 1.5144785762850468 1102 1 0.34610738806550145 1102 2 0.4270235151852866 1102 3 0.40973271162713587 1102 4 0.298567595407647 1102 5 0.2873900491573167 1102 6 0.26894113405924003 1102 7 0.282700781574448 1102 8 0.2384657793087328 1102 9 0.39934056722999145 1102 10-11 0.4218147529336562 1102 12-13 0.3869716270057637 1102 14-15 0.33174382648433465 1102 16-17 0.4436542782732502 1102 18-19 0.49886792429181526 1102 20-21 0.5022248398554083 1102 22-23 0.42327855992991914 1102 24-25 0.4815359381518576 1102 26-27 0.53354205380964 1102 28-29 0.47210087980828774 1102 30-31 0.40731443366230735 1102 32-33 0.26778076927168115 1102 34-35 0.29022712007364504 1102 36-37 0.5279950295180598 1102 38-39 0.5844508406281221 1102 40-41 0.5987660092056686 1102 42-43 0.5851233568108896 1102 44-45 0.6521649874267581 1102 46-47 0.30211328340506327 1102 48-49 0.5171349739249962 1102 50-51 0.43198033812986125 1102 52-53 0.2897091103619758 1102 54-55 0.3553313572536396 1102 56-57 0.4203148140033832 1102 58-59 0.5637420812523288 1102 60-61 0.502479729797173 1102 62-63 0.3536182855110539 1102 64-65 0.40770344261990843 1102 66-67 0.30555874250013204 1102 68-69 0.4038154464586938 1102 70-71 0.576380877790104 1102 72-73 0.46506956680595835 1102 74-75 0.4698929461861212 1102 76-77 0.37290162114278047 1102 78-79 0.24119859038576053 1102 80-81 0.2871943527279974 1102 82-83 0.40049352107045877 1102 84-85 0.48419188023650506 1102 86-87 0.38929690814188334 1102 88-89 0.4158735639321627 1102 90-91 0.3313739619317495 1102 92-93 0.5902260982817467 1102 94-95 0.4613325060280644 1102 96-97 0.46857017621620045 1102 98-99 0.34598460522743935 1102 100-101 -0.12941036958222796 1103 1 0.27877548016368436 1103 2 0.3026181425521379 1103 3 0.3258265748574587 1103 4 0.2884753728585636 1103 5 0.31377368635151015 1103 6 0.23758821778421435 1103 7 0.3721334550477877 1103 8 0.27351800128381853 1103 9 0.4329207202486316 1103 10-11 0.37483650033092886 1103 12-13 0.44793662740038087 1103 14-15 0.6089866688330474 1103 16-17 0.5977094037279684 1103 18-19 0.5485699827966926 1103 20-21 0.4484623344442795 1103 22-23 0.43887639212601215 1103 24-25 0.5813792243933307 1103 26-27 0.5488618551102249 1103 28-29 0.6174222476485411 1103 30-31 0.6950208043366857 1103 32-33 0.6985974147721237 1103 34-35 0.7470614782348335 1103 36-37 0.7005227668368619 1103 38-39 0.7317800100750986 1103 40-41 0.8115466410353562 1103 42-43 0.8517403366659053 1103 44-45 0.9482275318220132 1103 46-47 0.8726129497679906 1103 48-49 0.9166752363861619 1103 50-51 0.9221317663117858 1103 52-53 0.9693365011287298 1103 54-55 0.8343292621563165 1103 56-57 0.8176687969603833 1103 58-59 0.9780726904808006 1103 60-61 1.0323991426915882 1103 62-63 1.1517050742004002 1103 64-65 0.9806408121247578 1103 66-67 0.8201535707667276 1103 68-69 0.7209506400279899 1103 70-71 0.5847899670685166 1103 72-73 0.5123117992804467 1103 74-75 0.824286372459504 1103 76-77 0.6934661613813233 1103 78-79 0.7555916220346468 1103 80-81 0.7107235991865402 1103 82-83 0.9124099850436309 1103 84-85 0.838450775431415 1103 86-87 0.7067391315280496 1103 88-89 0.9842862048310437 1103 90-91 0.9796080770999218 1103 92-93 0.8831705112359565 1103 94-95 0.8715111872169103 1103 96-97 0.9699537536188032 1103 98-99 0.975900071876886 1103 100-101 0.23162141801891067 1104 1 0.3159939295503129 1104 2 0.24947025896810615 1104 3 0.3996855134475297 1104 4 0.27140900553825276 1104 5 0.1885646109094381 1104 6 0.26081421949076145 1104 7 0.20506469993777898 1104 8 0.17908235681937867 1104 9 0.3832244873752728 1104 10-11 0.28715398545327275 1104 12-13 0.3062093693712242 1104 14-15 0.3926386809917872 1104 16-17 0.3890541263387277 1104 18-19 0.45764381382979025 1104 20-21 0.46513701794706463 1104 22-23 0.41070234354513957 1104 24-25 0.44623651224397065 1104 26-27 0.4405803387314222 1104 28-29 0.5392760757932749 1104 30-31 0.521322480565054 1104 32-33 0.46040071958815076 1104 34-35 0.6031344329778747 1104 36-37 0.6902052138207395 1104 38-39 0.6973939683457431 1104 40-41 0.7893537179837509 1104 42-43 0.7064554158168121 1104 44-45 0.6922407964253594 1104 46-47 0.6383431617519335 1104 48-49 0.6799796841767076 1104 50-51 0.8486603400993786 1104 52-53 0.8816469379561553 1104 54-55 0.7486936296595843 1104 56-57 0.8232012769417949 1104 58-59 1.0094272461821774 1104 60-61 0.8637839341397324 1104 62-63 0.878961808774271 1104 64-65 0.8263815418908358 1104 66-67 0.7257953137036175 1104 68-69 0.560029580355689 1104 70-71 0.47498832047174844 1104 72-73 0.41575605647359026 1104 74-75 0.4734921590808625 1104 76-77 0.3608762553451612 1104 78-79 0.5713809821587859 1104 80-81 0.9422626763719855 1104 82-83 0.7552363167829981 1104 84-85 0.9274894397881575 1104 86-87 0.7920706804440805 1104 88-89 0.5868627737671055 1104 90-91 0.5759844479932816 1104 92-93 0.8783153867803257 1104 94-95 0.9510622712446697 1104 96-97 0.83558994997912 1104 98-99 0.5950005771101736 1104 100-101 0.5440845583133473 1105 1 0.30750735565964504 1105 2 0.1728038586184475 1105 3 0.10971909427138371 1105 4 0.16394566951134237 1105 5 0.20112611147769144 1105 6 0.195241074418675 1105 7 0.20239802811064322 1105 8 0.16033548247336427 1105 9 0.15929703765734615 1105 10-11 0.2488398605797073 1105 12-13 0.31778296285112617 1105 14-15 0.31086991400543695 1105 16-17 0.1887326383988821 1105 18-19 0.33235308826891696 1105 20-21 0.3508060205548489 1105 22-23 0.414995530372785 1105 24-25 0.4349742055645507 1105 26-27 0.5067799277554457 1105 28-29 0.37416925272388113 1105 30-31 0.454288277996973 1105 32-33 0.41900164852767574 1105 34-35 0.5812821548754599 1105 36-37 0.513794339572037 1105 38-39 0.4830895934392174 1105 40-41 0.5472600847940541 1105 42-43 0.39414698091211164 1105 44-45 0.25525052510546686 1105 46-47 0.12957018188853908 1105 48-49 0.11651021309057796 1105 50-51 0.12487664627510497 1105 52-53 0.2826592559010592 1105 54-55 0.3939843939239722 1105 56-57 0.4401602426513094 1105 58-59 0.36268440916514777 1105 60-61 0.4793484676808859 1105 62-63 0.5474777199253893 1105 64-65 0.3810478996666298 1105 66-67 0.2422979373869012 1105 68-69 0.198783460036978 1105 70-71 0.3789886876021846 1105 72-73 0.29344937152043826 1105 74-75 0.40098610958405345 1105 76-77 0.3729333662442649 1105 78-79 0.5220048412691618 1105 80-81 0.6682078229933239 1105 82-83 0.6322089171861194 1105 84-85 0.5860130722894858 1105 86-87 0.65486507938272 1105 88-89 0.546857443740894 1105 90-91 0.4894530758957991 1105 92-93 0.642443342795449 1105 94-95 0.5293836613199296 1105 96-97 0.293299868831685 1105 98-99 0.41498682077696714 1105 100-101 -0.12342160591172657 1106 1 0.15332886823641445 1106 2 0.20521641433701987 1106 3 0.22753133677311155 1106 4 0.13558107587692803 1106 5 0.06814885295506201 1106 6 0.06532672256809491 1106 7 0.1518169168779835 1106 8 0.2453283941421489 1106 9 0.11253851310764418 1106 10-11 0.14056759390234674 1106 12-13 0.13090594031303482 1106 14-15 0.3291785046011242 1106 16-17 0.13084963901508928 1106 18-19 0.32291755470364336 1106 20-21 0.27056234577045046 1106 22-23 0.3364265047585988 1106 24-25 0.23304966454704612 1106 26-27 0.2624536160568667 1106 28-29 0.3473351504573188 1106 30-31 0.432419855454377 1106 32-33 0.4788898018621097 1106 34-35 0.2846666887359177 1106 36-37 -0.15574513053191907 1106 38-39 0.21496377679694945 1106 40-41 0.2977850445700412 1106 42-43 0.5445050804367852 1106 44-45 0.3490650972192739 1106 46-47 0.02968449119263994 1106 48-49 0.2038268276002526 1106 50-51 0.3001585166930525 1106 52-53 0.27732569206182944 1106 54-55 -0.11637696529858488 1106 56-57 -0.04210106418520354 1106 58-59 0.14933828257123594 1106 60-61 -0.36488840846213577 1106 62-63 -0.2911889482071359 1106 64-65 -0.47401785223242854 1106 66-67 -0.2315670363623603 1106 68-69 -0.7970605625951066 1106 70-71 -0.493034914149586 1106 72-73 -0.24095019817745467 1106 74-75 -0.04625337156879539 1106 76-77 0.03159929778401249 1106 78-79 -0.27063907054418834 1106 80-81 -0.826314730213138 1106 82-83 -0.23638114529783394 1106 84-85 -0.1048528200019625 1106 86-87 -0.09495888044109435 1106 88-89 0.19495546774832562 1106 90-91 0.09868085181988207 1106 92-93 -0.2138831371101375 1106 94-95 -0.15762570055435887 1106 96-97 -0.24347040272368403 1106 98-99 -0.4529537757642359 1106 100-101 -0.9899435742415328 1107 1 0.16788418070050426 1107 2 0.17106393021445498 1107 3 0.22669220140778634 1107 4 0.1730434828293923 1107 5 0.14047874373736846 1107 6 0.19783747195717183 1107 7 0.16430799970953558 1107 8 0.18652820593297292 1107 9 0.27457457641357763 1107 10-11 0.2783920541561642 1107 12-13 0.31210795293692684 1107 14-15 0.4229456477897102 1107 16-17 0.3153396203459451 1107 18-19 0.3482005731624511 1107 20-21 0.4324187105146322 1107 22-23 0.3753701374124532 1107 24-25 0.324833076553837 1107 26-27 0.25716700904759193 1107 28-29 0.22828824127258684 1107 30-31 0.35260387979146657 1107 32-33 0.395937910121404 1107 34-35 0.41382306497212795 1107 36-37 0.38628292087433636 1107 38-39 0.2894706492505392 1107 40-41 0.274419081808567 1107 42-43 0.5215708820929379 1107 44-45 0.5826710592001518 1107 46-47 0.496194154259193 1107 48-49 0.265186974958489 1107 50-51 0.36102456076609 1107 52-53 0.5745492815229838 1107 54-55 0.3211529844617189 1107 56-57 0.3453978787644374 1107 58-59 0.1745825425167169 1107 60-61 0.21573688832508253 1107 62-63 0.32208483976265256 1107 64-65 0.27478226008594575 1107 66-67 0.5241255864018441 1107 68-69 0.30998909600472757 1107 70-71 0.1906481854039619 1107 72-73 0.24629987717126056 1107 74-75 0.08223435255298739 1107 76-77 -0.0875457273575222 1107 78-79 0.07347689926428203 1107 80-81 0.32278981260987294 1107 82-83 0.34171075411867236 1107 84-85 0.24617734333671493 1107 86-87 0.2632668144475545 1107 88-89 0.3143124450396293 1107 90-91 0.3283250432468243 1107 92-93 0.2881735034428665 1107 94-95 0.14447387250570998 1107 96-97 0.22458472218172076 1107 98-99 0.00732742131548747 1107 100-101 -0.3440619508723728 1108 1 0.19605681363566063 1108 2 0.24065503262321286 1108 3 0.1482406976029793 1108 4 0.09014456080516453 1108 5 0.06609958107046765 1108 6 0.06459822575614993 1108 7 0.23021614184892059 1108 8 0.1245673792376607 1108 9 0.19829353984462728 1108 10-11 0.2888706376786274 1108 12-13 0.21300840281751476 1108 14-15 0.4318709459971828 1108 16-17 0.2934934994270364 1108 18-19 0.30627246505548555 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-0.3645213014500044 2316 84-85 -0.3487214409848498 2316 86-87 -0.16641940786350418 2316 88-89 0.08796559053944009 2316 90-91 -0.10249122394802868 2316 92-93 0.02160784045291564 2316 94-95 0.0056492781861443575 2316 96-97 0.29951408017994297 2316 98-99 -0.02505091998305531 2316 100-101 0.11818432849743843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 18.0 4 49.0 5 97.0 6 218.0 7 504.0 8 1044.0 9 1767.0 10 2886.0 11 4414.0 12 6179.0 13 8909.0 14 11436.0 15 15032.0 16 19096.0 17 24070.0 18 22624.0 19 17415.0 20 17171.0 21 18442.0 22 19773.0 23 22260.0 24 25725.0 25 30213.0 26 35827.0 27 42678.0 28 51046.0 29 60135.0 30 71455.0 31 84921.0 32 101410.0 33 124135.0 34 156178.0 35 200276.0 36 249524.0 37 261937.0 38 111455.0 39 3390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92446286366335 19.45737756066105 15.35445268106195 22.263706894613637 2 16.336476426254524 20.943213130005997 38.75132409806362 23.968986345675855 3 19.49868727833428 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22.9174468981105 25.547496286418188 29.33603515030616 22.19902166516515 24-25 22.736654228617017 25.75740045676247 29.46819798158145 22.03774733303906 26-27 22.459397158891033 26.222691636663352 29.265495269251137 22.052415935194485 28-29 22.18059769338453 26.203905114352 29.665171729511968 21.9503254627515 30-31 22.289003027343696 25.8441989986352 29.554764981951635 22.312032992069465 32-33 22.196828335191267 25.9120551447022 29.360600446013652 22.53051607409288 34-35 22.869741703285342 25.896191054349195 29.042161793407594 22.19190544895787 36-37 22.924518111167956 25.60268661283132 29.070235437780916 22.402559838219805 38-39 22.41240047377772 25.937356057116535 29.20417406944353 22.446069399662214 40-41 22.52765044366559 25.801615418170893 29.32991046079092 22.340823677372605 42-43 22.67392223404798 25.74687325732315 28.952493909370112 22.62671059925876 44-45 22.44441276299412 26.077967439998684 28.66374039732194 22.81387939968526 46-47 22.72317269567382 26.32788309112573 28.238246081753083 22.710698131447362 48-49 22.88953677419284 26.32497692891034 28.035363059168915 22.750123237727905 50-51 22.659154877061113 26.56448856205835 27.77531637414042 23.001040186740113 52-53 23.080137148923267 26.62771129855553 27.550308135444705 22.7418434170765 54-55 22.553052539574526 26.409310466406133 27.787215189248737 23.250421804770603 56-57 22.794154336953607 26.835913146326362 27.675574693578092 22.694357823141935 58-59 23.0636323408698 26.249471544559416 27.666664290559197 23.02023182401159 60-61 22.828041285050098 26.373120521837066 28.06245068434637 22.736387508766466 62-63 22.7249547762776 26.47083337217355 27.896607521696147 22.907604329852703 64-65 22.501399618689586 26.398371233161395 27.84199360534646 23.25823554280256 66-67 22.595451031139817 26.624463319277737 27.622538671170304 23.15754697841214 68-69 22.40251533012337 26.44493575413021 27.78921059803246 23.363338317713964 70-71 22.40260852948345 26.589240227130475 27.68557711631156 23.322574127074517 72-73 22.607940622170947 26.636621701574427 27.376843151135237 23.378594525119386 74-75 22.427210232322995 26.81027860225661 27.205900496295737 23.55661066912466 76-77 22.4528996096421 26.676074224479645 27.501725876523196 23.369300289355056 78-79 22.60168963174538 26.621021642135183 27.339912957699987 23.437375768419447 80-81 22.270238436970768 27.067793064554024 27.387831097344144 23.27413740113106 82-83 22.57796097057577 26.902874977860954 27.206241593331367 23.312922458231913 84-85 22.470542915151256 26.53804025218966 27.123768423101758 23.867648409557322 86-87 22.584111228365416 26.292098110871393 27.397779698810886 23.726010961952305 88-89 22.400418488276898 26.46757401271147 27.18449290373947 23.947514595272157 90-91 22.27997245097491 26.54941332201542 27.44544207302027 23.725172153989398 92-93 22.504473066464588 26.25208848199779 27.2927789929555 23.95065945858212 94-95 22.26299685805309 26.121176064176872 27.276485844788915 24.33934123298112 96-97 22.509843939089034 26.082613966796274 27.088036426824207 24.319505667290485 98-99 22.313126710456547 26.209938098676925 26.8841136387439 24.592821552122622 100-101 22.349401851499497 25.952549499345018 27.236689365804125 24.461359283351364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1572.0 1 1025.5 2 677.0 3 927.0 4 833.0 5 753.0 6 1268.0 7 2871.0 8 4355.5 9 4558.0 10 3696.0 11 2733.5 12 2554.5 13 2711.0 14 2918.5 15 3121.0 16 3264.5 17 3387.0 18 3604.0 19 3797.0 20 4021.0 21 4421.5 22 4989.0 23 5715.0 24 6474.5 25 7693.0 26 9358.5 27 11130.0 28 12547.0 29 14221.0 30 16391.0 31 18890.0 32 22059.0 33 25266.5 34 28386.5 35 30523.0 36 33213.5 37 36706.5 38 39258.5 39 40725.0 40 43256.0 41 48868.5 42 54511.0 43 58779.0 44 62317.5 45 65793.5 46 66508.0 47 67713.0 48 74155.0 49 76836.0 50 73872.0 51 70323.0 52 66872.0 53 66280.5 54 66668.5 55 62599.5 56 56395.0 57 51796.5 58 47363.5 59 41851.5 60 35737.5 61 29329.5 62 24178.5 63 19542.0 64 15558.0 65 12755.5 66 9937.0 67 8088.5 68 6039.5 69 3820.0 70 2837.5 71 2348.0 72 2062.5 73 1870.0 74 1579.5 75 1292.5 76 870.0 77 508.0 78 313.5 79 187.5 80 110.0 81 62.5 82 38.5 83 21.0 84 8.5 85 8.0 86 5.5 87 3.0 88 4.5 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.579989921648771E-4 2 0.04233126849594042 3 0.0010418317375943886 4 0.0 5 0.08586886847751644 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.2899949608243854E-4 26-27 0.008910403018899376 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010308650877249739 36-37 1.0966649869414618E-4 38-39 0.0038931607036421893 40-41 0.005291408561992552 42-43 4.386659947765847E-4 44-45 5.483324934707309E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.483324934707309E-5 68-69 7.676654908590231E-4 70-71 8.224987402060964E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011789148609620713 82-83 5.483324934707309E-4 84-85 0.004578576320480602 86-87 0.002165913349209387 88-89 2.1933299738829235E-4 90-91 0.0029061622153948733 92-93 9.869984882473154E-4 94-95 5.483324934707309E-5 96-97 0.0 98-99 1.0966649869414618E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1823711.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.07382079279181 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8374322118941 35.22883872535407 2 13.424916502184923 12.639242271640928 3 4.723900475601765 6.671161332943844 4 2.1307050252887385 4.012017060909721 5 1.1969345180613418 2.8172140501963114 6 0.7215647076686351 2.0380084643517917 7 0.4843258486659014 1.5959347743790813 8 0.33450774161896135 1.2597245986217993 9 0.28513672778840277 1.2080227702818895 >10 1.5890531250628277 14.203162817002982 >50 0.15966346095314796 5.2464376657806655 >100 0.10555478402341192 9.18398484930552 >500 0.004436761206168455 1.3897671485101626 >1k 0.0015178393600049978 1.0893452852753072 >5k 2.3351374769307659E-4 0.8453693154802978 >10k+ 1.1675687384653829E-4 0.5717688699656419 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10403 0.5704302929576013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8714 0.47781693481039483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6667 0.36557327339693624 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2093 0.11476599088342397 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1972 0.10813116771242813 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1939 0.10632167048397471 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1897 0.10401867401139764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6449974804121927E-4 0.0 22-23 0.0 0.0 0.0 3.838327454295116E-4 0.0 24-25 0.0 2.741662467353654E-5 0.0 8.224987402060962E-4 0.0 26-27 0.0 5.483324934707308E-5 0.0 0.0017820806037798752 0.0 28-29 0.0 5.483324934707308E-5 0.0 0.0044140765724393835 0.0 30-31 0.0 5.483324934707308E-5 0.0 0.013708312336768272 0.0 32-33 0.0 5.483324934707308E-5 0.0 0.030487286636972633 0.0 34-35 0.0 5.483324934707308E-5 0.0 0.05658791332617942 0.0 36-37 0.0 5.483324934707308E-5 0.0 0.10026259643112313 0.0 38-39 0.0 5.483324934707308E-5 0.0 0.16619957877097852 0.0 40-41 0.0 5.483324934707308E-5 0.0 0.23918263365193276 0.0 42-43 0.0 5.483324934707308E-5 0.0 0.3131526870211344 0.0 44-45 0.0 5.483324934707308E-5 0.0 0.4056289620449731 0.0 46-47 0.0 5.483324934707308E-5 0.0 0.5147197116209751 0.0 48-49 0.0 5.483324934707308E-5 0.0 0.6352706103105152 0.0 50-51 0.0 5.483324934707308E-5 0.0 0.7654173276357932 0.0 52-53 0.0 5.483324934707308E-5 0.0 0.8923837164989409 0.0 54-55 0.0 5.483324934707308E-5 0.0 1.0331955008222247 0.0 56-57 0.0 5.483324934707308E-5 0.0 1.2004369113307973 0.0 58-59 0.0 5.483324934707308E-5 0.0 1.3824010492890595 0.0 60-61 0.0 5.483324934707308E-5 0.0 1.5807603288020964 0.0 62-63 0.0 5.483324934707308E-5 0.0 1.7801066068033806 5.483324934707308E-5 64-65 0.0 5.483324934707308E-5 0.0 1.983400878757654 5.483324934707308E-5 66-67 0.0 5.483324934707308E-5 0.0 2.221623930546013 5.483324934707308E-5 68-69 0.0 5.483324934707308E-5 0.0 2.489621436729833 5.483324934707308E-5 70-71 0.0 5.483324934707308E-5 0.0 2.7808134073874644 5.483324934707308E-5 72-73 0.0 5.483324934707308E-5 0.0 3.0716215452996662 5.483324934707308E-5 74-75 0.0 5.483324934707308E-5 0.0 3.3716416691021767 5.483324934707308E-5 76-77 0.0 5.483324934707308E-5 0.0 3.7006959984339627 5.483324934707308E-5 78-79 0.0 5.483324934707308E-5 0.0 4.05069662901633 5.483324934707308E-5 80-81 0.0 5.483324934707308E-5 0.0 4.43924503388969 5.483324934707308E-5 82-83 0.0 5.483324934707308E-5 0.0 4.841063085105041 5.483324934707308E-5 84-85 0.0 5.483324934707308E-5 0.0 5.24200380433084 5.483324934707308E-5 86-87 2.741662467353654E-5 5.483324934707308E-5 0.0 5.664877823295468 5.483324934707308E-5 88-89 5.483324934707308E-5 5.483324934707308E-5 0.0 6.115881299175143 5.483324934707308E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5900 0.0 47.16439 1 GTATCAA 9775 0.0 38.054005 1 TATCAAC 12775 0.0 29.00594 2 ATCAACG 12950 0.0 28.525621 3 TCAACGC 13215 0.0 27.952831 4 CAACGCA 13455 0.0 27.45423 5 AACGCAG 13875 0.0 26.631866 6 GTACATG 11360 0.0 25.2493 1 TACATGG 11695 0.0 24.363348 2 ACGCAGA 15580 0.0 23.625948 7 ACATGGG 11685 0.0 23.476372 3 GAGTACT 8430 0.0 23.184477 12-13 CGCAGAG 15850 0.0 23.163555 8 GTAGCGC 370 0.0 23.128883 3 TAGCGCG 320 0.0 22.285028 4 AGTACTT 8760 0.0 21.768898 12-13 GTACTTT 8925 0.0 21.738962 14-15 GCAGAGT 16750 0.0 21.663746 9 CATGGGG 4730 0.0 21.207684 4 CATGGGA 6600 0.0 20.529242 4 >>END_MODULE