FastQCFastQC Report
Thu 26 May 2016
SRR938329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832404
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113820.6211512308421069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83460.45546724412302086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62680.3420643045965846No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.1089825169558678No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG19190.1047258137397648No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18690.10199715783200647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.046.5513461
GTATCAA96850.039.795731
TATCAAC123600.031.2216782
ATCAACG126150.030.4775433
TCAACGC128300.029.9668124
CAACGCA130800.029.3213845
AACGCAG133900.028.639426
GTACATG111600.028.4740491
TACATGG114600.027.162852
ACATGGG119150.025.447513
ACGCAGA152200.025.0641827
CGCAGAG154650.024.6056758
GAGTACT81400.023.63392312-13
GCAGAGT160100.023.2326839
TTAGGGC7400.023.1200873
CATGGGG51250.022.6263584
AGTACTT83550.022.25822412-13
CATGGGA65900.022.2118114
GTACTTT88250.021.93521114-15
AGAGTAC146450.020.48307410-11
Produced by FastQC (version 0.11.5)