##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938329_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832404 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2852198532638 31.0 31.0 34.0 30.0 34.0 2 31.426270080178824 31.0 31.0 34.0 28.0 34.0 3 31.478075249781163 33.0 31.0 34.0 28.0 34.0 4 35.152536231093144 37.0 35.0 37.0 32.0 37.0 5 35.16754765870409 37.0 35.0 37.0 32.0 37.0 6 35.18843224529088 37.0 35.0 37.0 32.0 37.0 7 35.109456211621456 37.0 35.0 37.0 32.0 37.0 8 35.08097941283691 37.0 35.0 37.0 32.0 37.0 9 36.574005514067856 39.0 37.0 39.0 32.0 39.0 10-11 36.442823471243244 39.0 37.0 39.0 32.0 39.0 12-13 36.272250551734224 39.0 35.0 39.0 32.0 39.0 14-15 37.26035852355703 39.0 36.5 41.0 31.5 41.0 16-17 37.117844918478674 39.0 36.0 41.0 31.0 41.0 18-19 37.01107697865754 39.0 36.0 41.0 31.0 41.0 20-21 37.020291103926866 39.0 36.0 41.0 31.0 41.0 22-23 37.101730022418636 39.0 36.0 41.0 31.0 41.0 24-25 37.005199999563416 39.0 36.0 41.0 31.0 41.0 26-27 36.821073300429376 39.0 36.0 41.0 30.0 41.0 28-29 36.6379627527554 39.0 36.0 41.0 30.0 41.0 30-31 36.352530337196384 39.0 35.5 40.0 30.0 41.0 32-33 36.055901973582245 38.0 35.0 40.0 29.5 41.0 34-35 35.774184077310466 38.0 35.0 40.0 28.5 41.0 36-37 35.49067509130083 38.0 34.5 40.0 27.0 41.0 38-39 35.18557370536192 38.0 34.0 40.0 26.5 41.0 40-41 35.05541736429303 38.0 34.0 40.0 26.0 41.0 42-43 34.80605559690986 38.0 34.0 40.0 24.5 41.0 44-45 34.450467527903236 38.0 33.0 40.0 24.0 41.0 46-47 33.976704645918694 38.0 33.0 40.0 22.5 41.0 48-49 33.54412645901231 38.0 33.0 40.0 20.5 41.0 50-51 32.74553864759082 36.5 31.5 39.5 18.0 40.5 52-53 32.77899906352529 37.0 31.5 39.0 18.0 40.0 54-55 32.8532812633022 37.0 32.0 40.0 16.0 41.0 56-57 32.54486892628482 37.0 31.0 40.0 13.0 41.0 58-59 32.10824468839841 36.0 31.0 39.0 8.5 41.0 60-61 31.638911506414523 35.5 30.5 39.0 7.5 41.0 62-63 31.068956409176145 35.0 29.5 39.0 4.0 40.0 64-65 30.56908547460058 35.0 29.0 38.5 2.0 40.0 66-67 30.02477865143276 34.5 28.0 38.0 2.0 40.0 68-69 29.417228951694057 34.0 27.5 37.5 2.0 39.5 70-71 28.791807920087493 34.0 26.0 37.0 2.0 39.0 72-73 28.17063076701426 34.0 26.0 36.0 2.0 39.0 74-75 27.474450776138887 33.0 25.5 35.5 2.0 38.5 76-77 26.759020117834275 32.5 23.5 35.0 2.0 37.5 78-79 25.983785235133738 32.0 21.0 35.0 2.0 37.0 80-81 25.234034634283706 31.5 18.5 35.0 2.0 36.0 82-83 24.52848089176841 31.0 14.0 34.5 2.0 36.0 84-85 23.800183529396357 31.0 7.0 34.0 2.0 35.0 86-87 23.080062584451902 30.0 2.0 34.0 2.0 35.0 88-89 22.33673824113023 29.5 2.0 34.0 2.0 35.0 90-91 21.53500565377504 29.0 2.0 34.0 2.0 35.0 92-93 20.667694733257512 28.5 2.0 34.0 2.0 35.0 94-95 19.780919764418762 27.0 2.0 34.0 2.0 35.0 96-97 18.710695348842286 25.5 2.0 33.5 2.0 35.0 98-99 17.463252099427855 22.0 2.0 33.0 2.0 35.0 100-101 14.646603587418495 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.517687102457888 1101 2 0.5381857143753344 1101 3 0.4540662195659344 1101 4 0.5088051261652708 1101 5 0.40775223365617563 1101 6 0.42409547227521927 1101 7 0.2619157076921397 1101 8 0.36553620071765636 1101 9 0.4170742590130345 1101 10-11 0.5033653945659537 1101 12-13 0.5943384383615964 1101 14-15 0.6317267271184122 1101 16-17 0.7492232421236338 1101 18-19 0.7451968225704064 1101 20-21 0.8220007494272323 1101 22-23 0.6460031587547448 1101 24-25 0.7563765899981973 1101 26-27 0.8598010224161001 1101 28-29 0.8529633245453923 1101 30-31 0.7560268988437215 1101 32-33 0.9110992260668169 1101 34-35 1.2047136061027786 1101 36-37 1.1310301569622183 1101 38-39 1.3228157746078182 1101 40-41 1.1866211050217998 1101 42-43 1.233735254906513 1101 44-45 1.1843981037584612 1101 46-47 1.4937944726547627 1101 48-49 1.310539902337709 1101 50-51 1.5795016684475058 1101 52-53 1.5014316498423455 1101 54-55 1.7618443889515731 1101 56-57 1.7503464964836084 1101 58-59 1.9177514083564446 1101 60-61 1.9698925438817305 1101 62-63 1.8419611362501591 1101 64-65 1.896397522611295 1101 66-67 1.9966465100789854 1101 68-69 2.1983418852138605 1101 70-71 2.369159636692217 1101 72-73 2.343109430290962 1101 74-75 2.534692958439699 1101 76-77 2.6998049804598097 1101 78-79 2.891638636002316 1101 80-81 3.285276640234418 1101 82-83 3.407096790911382 1101 84-85 3.6293955447995963 1101 86-87 4.017224185706024 1101 88-89 4.361954659776668 1101 90-91 4.3403638063771055 1101 92-93 4.462807355940047 1101 94-95 4.864577845889585 1101 96-97 5.007850137517224 1101 98-99 5.178365779847116 1101 100-101 5.288159522733025 1102 1 0.40024277746376313 1102 2 0.39763632316383024 1102 3 0.4063558495910087 1102 4 0.38043517817921924 1102 5 0.35567369602198085 1102 6 0.25884374092579776 1102 7 0.41629140561941824 1102 8 0.4885461896487726 1102 9 0.4505071184088507 1102 10-11 0.42341797787643287 1102 12-13 0.5686542553528184 1102 14-15 0.9106926988780728 1102 16-17 0.7885702699329755 1102 18-19 0.7038902414834922 1102 20-21 0.8449302624454091 1102 22-23 0.6360254005951234 1102 24-25 0.6054167563252832 1102 26-27 0.6871876888215525 1102 28-29 0.7966187064218175 1102 30-31 0.8545604556770527 1102 32-33 0.8281804146002472 1102 34-35 0.8491720022903806 1102 36-37 0.9002366258909831 1102 38-39 1.1218399620735369 1102 40-41 1.1876712100782214 1102 42-43 1.1574418548497007 1102 44-45 1.43869234622575 1102 46-47 1.4675135312120844 1102 48-49 1.5210532890726824 1102 50-51 1.6152906446249204 1102 52-53 1.5984461434654946 1102 54-55 1.5748871192430869 1102 56-57 1.6860513234466126 1102 58-59 1.7034340118455944 1102 60-61 1.6912388027466285 1102 62-63 1.9643817133503028 1102 64-65 2.143359090082498 1102 66-67 2.014378454577855 1102 68-69 2.246659727418624 1102 70-71 2.283354222490747 1102 72-73 2.40861709891999 1102 74-75 2.4385132225745636 1102 76-77 2.8034355491848295 1102 78-79 3.267475230411062 1102 80-81 3.6810794647583585 1102 82-83 3.880339391657799 1102 84-85 3.765083780684325 1102 86-87 3.9397778281517546 1102 88-89 4.355094463181569 1102 90-91 4.74051107515502 1102 92-93 4.505050475846399 1102 94-95 4.608131814034714 1102 96-97 4.605866677169288 1102 98-99 4.926034763958864 1102 100-101 5.122800194261508 1103 1 0.49765119407279457 1103 2 0.5306886258660164 1103 3 0.4639216155286654 1103 4 0.4161178202646454 1103 5 0.4062431109853719 1103 6 0.42147998314478485 1103 7 0.6239159017915128 1103 8 0.4691804165561635 1103 9 0.7382262388267051 1103 10-11 0.5627234108702979 1103 12-13 0.6742127590137699 1103 14-15 0.7811856750997777 1103 16-17 0.9671847133969322 1103 18-19 1.0470553241231926 1103 20-21 0.960823051445864 1103 22-23 0.9278475881025727 1103 24-25 0.9212154875137202 1103 26-27 1.0922544385651634 1103 28-29 1.1750639650733419 1103 30-31 1.0534599345580133 1103 32-33 1.2501204799488121 1103 34-35 1.1681986604506065 1103 36-37 1.2776140078938951 1103 38-39 1.3047111550425967 1103 40-41 1.2253463573509933 1103 42-43 1.4163706392232882 1103 44-45 1.2805282473919988 1103 46-47 1.3898202878721548 1103 48-49 1.339822219884283 1103 50-51 1.4867803951555913 1103 52-53 1.6896619487553863 1103 54-55 1.9338989308770493 1103 56-57 1.9748102969494425 1103 58-59 2.031020527145266 1103 60-61 2.071850521366205 1103 62-63 2.2202972193246886 1103 64-65 2.4436121965243416 1103 66-67 2.4444483346131527 1103 68-69 2.6074233099033 1103 70-71 2.7659545706984296 1103 72-73 2.7630021903841318 1103 74-75 3.069342123812369 1103 76-77 3.3947486916399328 1103 78-79 3.3976993891079026 1103 80-81 3.558660762905223 1103 82-83 3.819958301004551 1103 84-85 3.884221340607052 1103 86-87 4.0948299698675115 1103 88-89 4.413667586795302 1103 90-91 4.729423880134867 1103 92-93 4.787411878455572 1103 94-95 4.879513793094553 1103 96-97 5.0879840660886515 1103 98-99 5.391278241027241 1103 100-101 5.312982407049791 1104 1 0.5604535718617711 1104 2 0.511137152919666 1104 3 0.5741509186853051 1104 4 0.630054114762288 1104 5 0.5329680388270361 1104 6 0.5237604156366515 1104 7 0.5861662110415296 1104 8 0.5436048679237331 1104 9 0.7014907735772411 1104 10-11 0.7533674526534142 1104 12-13 0.7059217662066928 1104 14-15 0.7627360484905381 1104 16-17 0.8500992229493249 1104 18-19 1.0008989008859217 1104 20-21 0.8564657760529997 1104 22-23 0.8502745134702323 1104 24-25 0.9236379459367754 1104 26-27 0.9399370678774872 1104 28-29 0.9318677516679301 1104 30-31 0.9406767417234505 1104 32-33 1.0190385630568883 1104 34-35 1.0895389156391317 1104 36-37 1.2531182572181194 1104 38-39 1.2002731310356367 1104 40-41 1.3233766065835937 1104 42-43 1.5211892125499062 1104 44-45 1.5667328334551343 1104 46-47 1.7412794897780515 1104 48-49 1.8230774771344826 1104 50-51 1.6788896520392598 1104 52-53 1.6501346431247441 1104 54-55 1.7291402031545005 1104 56-57 2.003622089683212 1104 58-59 2.077184681632829 1104 60-61 2.092695976536408 1104 62-63 2.217692933348605 1104 64-65 2.209959495740904 1104 66-67 2.3431655197508228 1104 68-69 2.5957694522957517 1104 70-71 2.782993100959118 1104 72-73 3.0040780368128672 1104 74-75 3.047107047988849 1104 76-77 3.2505691777351338 1104 78-79 3.328667627986359 1104 80-81 3.677661599025626 1104 82-83 4.029007013608208 1104 84-85 4.140385437826094 1104 86-87 4.163114949479919 1104 88-89 4.407815316671144 1104 90-91 4.645611400178851 1104 92-93 4.687219742568825 1104 94-95 4.944762109400049 1104 96-97 4.975813360082334 1104 98-99 5.305554996880087 1104 100-101 5.184371054138261 1105 1 0.4948370844436951 1105 2 0.5229455367277112 1105 3 0.4696926630106155 1105 4 0.4402071497592388 1105 5 0.471593771144164 1105 6 0.34050818890739976 1105 7 0.45817077706986 1105 8 0.4692335554031928 1105 9 0.5188662667175663 1105 10-11 0.6187230397418446 1105 12-13 0.6753016836409529 1105 14-15 0.7676136199478378 1105 16-17 0.8623830984258305 1105 18-19 0.7581373041497415 1105 20-21 0.8842534873530994 1105 22-23 0.9602946179502965 1105 24-25 0.8819349144014197 1105 26-27 0.9012867773492133 1105 28-29 0.9913577694774034 1105 30-31 0.9320004156754464 1105 32-33 0.9777397471388056 1105 34-35 1.2424039675875136 1105 36-37 1.380127137968799 1105 38-39 1.3272393551820585 1105 40-41 1.281291301006945 1105 42-43 1.371241981358743 1105 44-45 1.3983736217818858 1105 46-47 1.3657329264830125 1105 48-49 1.4068550478831128 1105 50-51 1.439368594395333 1105 52-53 1.705216448406759 1105 54-55 1.7763870941285802 1105 56-57 1.8726346969584355 1105 58-59 1.7986688098326837 1105 60-61 2.1789692993414214 1105 62-63 2.4540441828191426 1105 64-65 2.4623612124716203 1105 66-67 2.347878461246676 1105 68-69 2.3113579744256683 1105 70-71 2.499957809637678 1105 72-73 2.6353309089787906 1105 74-75 2.7580491347662814 1105 76-77 2.9933652540907296 1105 78-79 3.1917431991351215 1105 80-81 3.6115427721721893 1105 82-83 3.667484251880637 1105 84-85 4.058758414090487 1105 86-87 4.49251674307429 1105 88-89 4.650694116856705 1105 90-91 4.869218409926361 1105 92-93 4.8680323658709455 1105 94-95 5.046149907267161 1105 96-97 5.180120641360254 1105 98-99 5.373534224067797 1105 100-101 5.334110862480639 1106 1 0.17390438087083737 1106 2 0.14121596916742618 1106 3 0.14270557933123484 1106 4 0.1593707084798126 1106 5 0.21628525710723778 1106 6 0.03173917605229093 1106 7 0.262071061533355 1106 8 0.24994852101423248 1106 9 0.20039751056008726 1106 10-11 0.06528863974556032 1106 12-13 -0.09313576759455344 1106 14-15 0.45189699139092454 1106 16-17 0.22956154795905093 1106 18-19 0.36040068690140004 1106 20-21 0.30649115934912885 1106 22-23 0.3179245880336694 1106 24-25 0.5481275663396019 1106 26-27 0.446971330317389 1106 28-29 0.2511648458928377 1106 30-31 0.43537686535568554 1106 32-33 0.5311380733707978 1106 34-35 0.4916981379305625 1106 36-37 0.4375942841978997 1106 38-39 0.5050104556696269 1106 40-41 0.5837607961300151 1106 42-43 0.1956174064894114 1106 44-45 0.44771829539494234 1106 46-47 0.8395687892157753 1106 48-49 0.8158371145764036 1106 50-51 0.25948690541075337 1106 52-53 0.7247348144619963 1106 54-55 0.44491046651176447 1106 56-57 0.5792540140766178 1106 58-59 -0.15899589491311872 1106 60-61 1.1390120030263269 1106 62-63 1.405198256325999 1106 64-65 1.587071221766081 1106 66-67 1.593477940254072 1106 68-69 1.1552191079234646 1106 70-71 1.4723453927200651 1106 72-73 1.864827278236401 1106 74-75 2.2428469126224044 1106 76-77 2.3055216480697993 1106 78-79 2.565644076629436 1106 80-81 3.021885257036921 1106 82-83 3.1763033307479986 1106 84-85 3.3885391648565744 1106 86-87 3.5357107032330006 1106 88-89 3.434682366647845 1106 90-91 3.8988955032773376 1106 92-93 4.30973798263803 1106 94-95 4.154370243633149 1106 96-97 4.645881583257172 1106 98-99 4.73447327880946 1106 100-101 4.020425159524585 1107 1 0.3101223269945841 1107 2 0.18484992621686303 1107 3 0.301787450231334 1107 4 0.29146589061149797 1107 5 0.3173225176558816 1107 6 0.13854819827113118 1107 7 0.26959083628545244 1107 8 0.13384877823844477 1107 9 0.131947649316551 1107 10-11 0.2821714945404139 1107 12-13 0.3680213258743663 1107 14-15 0.38966149337041855 1107 16-17 0.5636507168609768 1107 18-19 0.6242683742630959 1107 20-21 0.5728720033488699 1107 22-23 0.5427468864105194 1107 24-25 0.4773119923767126 1107 26-27 0.5605753183448883 1107 28-29 0.4851605032445079 1107 30-31 0.6339310785825276 1107 32-33 0.6296859193945608 1107 34-35 0.7164802365930782 1107 36-37 0.763814156670378 1107 38-39 0.8831571625036361 1107 40-41 0.8410542114168109 1107 42-43 0.8588751913394645 1107 44-45 0.9699678929931039 1107 46-47 0.8621989351164601 1107 48-49 0.8516638284467035 1107 50-51 1.039028974007124 1107 52-53 1.1710143713694094 1107 54-55 1.4035848903743116 1107 56-57 1.4308157594888584 1107 58-59 1.4199211355744552 1107 60-61 1.4767026886176104 1107 62-63 1.8541224957824731 1107 64-65 1.7766244761352965 1107 66-67 1.9669668761595425 1107 68-69 2.135116552786453 1107 70-71 2.2841030807130878 1107 72-73 2.5889709029124433 1107 74-75 2.6268175867958945 1107 76-77 2.832131835486802 1107 78-79 2.9202626191118988 1107 80-81 3.3357899874910935 1107 82-83 3.5216101085277565 1107 84-85 3.7981688580383235 1107 86-87 4.114399350779713 1107 88-89 4.128033954428645 1107 90-91 4.250513501309982 1107 92-93 4.407789537557587 1107 94-95 4.32961482786828 1107 96-97 4.451671502248001 1107 98-99 4.414182324608536 1107 100-101 4.52231535548875 1108 1 0.19851436542829148 1108 2 0.2962896488839206 1108 3 0.31321829072048146 1108 4 0.24971756451017058 1108 5 0.22190807262219892 1108 6 0.19481757905271024 1108 7 0.10905017289125851 1108 8 0.3152481914922234 1108 9 0.3601571851182612 1108 10-11 0.4602617740416619 1108 12-13 0.4193853932848768 1108 14-15 0.4228736546394174 1108 16-17 0.5270951993304323 1108 18-19 0.5776659379339435 1108 20-21 0.6108710054100541 1108 22-23 0.6135573067726483 1108 24-25 0.5164200910942895 1108 26-27 0.49748142066235346 1108 28-29 0.675911151774109 1108 30-31 0.7172866609007258 1108 32-33 0.8504401730434381 1108 34-35 0.8970189929314216 1108 36-37 0.9370929081496513 1108 38-39 1.0085634568328814 1108 40-41 1.0398764085018897 1108 42-43 1.053263222343496 1108 44-45 1.1192430811515948 1108 46-47 1.31846478915093 1108 48-49 1.4752761840786661 1108 50-51 1.4949485537233613 1108 52-53 1.551266808090432 1108 54-55 1.5346821789588887 1108 56-57 1.6803729567959849 1108 58-59 1.9030793506746804 1108 60-61 2.022154229806109 1108 62-63 2.103625224439778 1108 64-65 2.2817515827135963 1108 66-67 2.2370314548177497 1108 68-69 2.4832704966040673 1108 70-71 2.727207767629377 1108 72-73 2.746665873248581 1108 74-75 2.8075134793110905 1108 76-77 2.970883602125866 1108 78-79 3.157210258673121 1108 80-81 3.424917156767119 1108 82-83 3.624769682078721 1108 84-85 3.8942437191236756 1108 86-87 3.8814827831667458 1108 88-89 4.17175582208942 1108 90-91 4.184730785505714 1108 92-93 4.41153272244534 1108 94-95 4.648759317391228 1108 96-97 4.6391349612293205 1108 98-99 4.928090900873787 1108 100-101 5.104158877638028 1109 1 0.45896766020305435 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2311 28-29 0.3519341035563244 2311 30-31 0.2987094174949263 2311 32-33 0.40455485789944134 2311 34-35 0.27523212342439507 2311 36-37 0.43681295353482597 2311 38-39 0.4676217143699688 2311 40-41 0.3933185146280991 2311 42-43 0.6131300025612703 2311 44-45 0.7960609347711625 2311 46-47 0.6951865034200111 2311 48-49 0.8037306547568193 2311 50-51 0.9431562887754694 2311 52-53 1.0182988131050195 2311 54-55 1.1105970896828552 2311 56-57 1.190937044847466 2311 58-59 1.121366060169862 2311 60-61 1.1594131871636115 2311 62-63 1.32166265927793 2311 64-65 1.4333917702845085 2311 66-67 1.3476855991246381 2311 68-69 1.2665165948275394 2311 70-71 1.582012751535622 2311 72-73 1.597683072538711 2311 74-75 1.6074710298867387 2311 76-77 1.791841054843026 2311 78-79 1.934668556411534 2311 80-81 1.9658049448215031 2311 82-83 1.8734279628902577 2311 84-85 2.241607755336606 2311 86-87 2.701103012896155 2311 88-89 2.6199212399583764 2311 90-91 2.5618673404109344 2311 92-93 2.9530550037646854 2311 94-95 3.224092499087991 2311 96-97 3.0746955448890674 2311 98-99 3.278727377756706 2311 100-101 3.4134904777300683 2312 1 -0.4367865793402963 2312 2 -0.47623039922102706 2312 3 -0.2840316922117161 2312 4 -0.3088386568356114 2312 5 -0.24947289651396432 2312 6 -0.12769642967550254 2312 7 -0.1093567970746605 2312 8 -0.25880034409021135 2312 9 -0.15133496106616207 2312 10-11 -0.36299192704596095 2312 12-13 -0.42181996127906274 2312 14-15 -0.4163279397560373 2312 16-17 -0.2616676212160485 2312 18-19 -0.36582020038353846 2312 20-21 -0.3221689805155634 2312 22-23 -0.30301907558817476 2312 24-25 -0.5782042071563964 2312 26-27 -0.3871463961817341 2312 28-29 -0.44122978102075905 2312 30-31 -0.2801514617458878 2312 32-33 -0.30926930667738617 2312 34-35 -0.2925833009814127 2312 36-37 -0.1299059680011112 2312 38-39 -0.022097277240234803 2312 40-41 -0.10376544070275173 2312 42-43 0.01660722524238878 2312 44-45 -0.05393184545685159 2312 46-47 -0.12582340465529995 2312 48-49 0.23737935763984552 2312 50-51 0.26220279047743134 2312 52-53 0.14234139701893866 2312 54-55 0.37183471786035227 2312 56-57 0.2776404014044047 2312 58-59 0.5291345348231502 2312 60-61 0.4511341790753356 2312 62-63 0.560877859446105 2312 64-65 0.6502957515640659 2312 66-67 0.7890514950452534 2312 68-69 0.8984481067964332 2312 70-71 1.0594618468344876 2312 72-73 1.0207704700020237 2312 74-75 1.109296145774696 2312 76-77 1.309475613343352 2312 78-79 1.4873102497453452 2312 80-81 1.6026339572628707 2312 82-83 1.913340030314437 2312 84-85 2.0297334600606725 2312 86-87 2.4458471781524906 2312 88-89 2.524040544566926 2312 90-91 2.5135949362905663 2312 92-93 2.934000910684837 2312 94-95 3.091639942545495 2312 96-97 3.310571335082493 2312 98-99 3.196508242812797 2312 100-101 3.105826795943642 2313 1 -0.3212235386428155 2313 2 -0.3506371047113177 2313 3 -0.40175015923968616 2313 4 -0.30752793882302143 2313 5 -0.3517709659925359 2313 6 -0.19853516297068552 2313 7 -0.18108231631254768 2313 8 -0.23281530689358476 2313 9 -0.2551941104249025 2313 10-11 -0.3075657017455242 2313 12-13 -0.24784265794988158 2313 14-15 -0.3373571794975163 2313 16-17 -0.24522841917612936 2313 18-19 -0.2367539239167158 2313 20-21 -0.03774474969454644 2313 22-23 -0.28646994575345275 2313 24-25 -0.1026507811961892 2313 26-27 -0.10038633121387619 2313 28-29 -0.008008023522521057 2313 30-31 -0.03414252445838173 2313 32-33 -0.013424156544417087 2313 34-35 -0.014868873405411875 2313 36-37 -0.13217973765137003 2313 38-39 -0.054937811457755004 2313 40-41 -0.16770870323346543 2313 42-43 0.06151720016412554 2313 44-45 -0.06734461433286754 2313 46-47 -0.0396968183288422 2313 48-49 0.1020861186725952 2313 50-51 0.33495395181987675 2313 52-53 0.3374011682849698 2313 54-55 0.2548094797009952 2313 56-57 0.33182428483254967 2313 58-59 0.5514009619278752 2313 60-61 0.5936163802400465 2313 62-63 0.5178292934515589 2313 64-65 0.612457973430967 2313 66-67 0.7948113959103686 2313 68-69 0.9954044218063665 2313 70-71 1.1693691287882402 2313 72-73 1.1804603158296025 2313 74-75 1.4590516747745923 2313 76-77 1.513750384441071 2313 78-79 1.6745993356510276 2313 80-81 1.7561215611946075 2313 82-83 2.132447164154943 2313 84-85 2.246761433265636 2313 86-87 2.2029220047926756 2313 88-89 2.3009092118843952 2313 90-91 2.5554774261077853 2313 92-93 2.2820906917128774 2313 94-95 2.5600733084188576 2313 96-97 3.092031197236196 2313 98-99 3.185382392844776 2313 100-101 3.050495771269322 2314 1 -0.39025556720353194 2314 2 -0.42578791756704604 2314 3 -0.4052797764988121 2314 4 -0.3667134641970691 2314 5 -0.3039869351142386 2314 6 -0.2427363234628288 2314 7 -0.31463263838745803 2314 8 -0.20544106947836838 2314 9 -0.31692833045830326 2314 10-11 -0.3758047912448603 2314 12-13 -0.32650924225170996 2314 14-15 -0.3060917360906785 2314 16-17 -0.18632247271895608 2314 18-19 -0.3001363282315026 2314 20-21 -0.2918553585694994 2314 22-23 -0.28577552497706193 2314 24-25 -0.3192030246439188 2314 26-27 -0.22630121993614694 2314 28-29 -0.2467875896586449 2314 30-31 -0.275607113259035 2314 32-33 -0.20216868212296646 2314 34-35 -0.22688235283693103 2314 36-37 -0.10073216855010969 2314 38-39 -0.15482683194103686 2314 40-41 -0.03858691120183977 2314 42-43 0.030388084762471124 2314 44-45 0.015501807909039655 2314 46-47 0.12250581620985912 2314 48-49 0.34239724474191746 2314 50-51 0.04049717631801997 2314 52-53 0.12698794532052204 2314 54-55 0.2092045667805067 2314 56-57 0.3882944332596665 2314 58-59 0.6142258060027217 2314 60-61 0.7750678900727088 2314 62-63 0.811493662423306 2314 64-65 0.8535993157505892 2314 66-67 1.0429704353091651 2314 68-69 1.3521630956756958 2314 70-71 1.2914087874569944 2314 72-73 1.2823154417626021 2314 74-75 1.519464152195269 2314 76-77 1.7273726408399703 2314 78-79 2.0161409284733587 2314 80-81 2.189615860979888 2314 82-83 2.500303257164724 2314 84-85 2.5960891657900937 2314 86-87 2.573518503719093 2314 88-89 2.8724366819774403 2314 90-91 2.8940379088793335 2314 92-93 2.9691737197282855 2314 94-95 3.191800243424481 2314 96-97 3.0444809475855337 2314 98-99 3.0914177827428695 2314 100-101 2.9834447590338833 2315 1 -0.49666406918165507 2315 2 -0.3844874886083893 2315 3 -0.45374486768542255 2315 4 -0.41393400508849965 2315 5 -0.29329367125642136 2315 6 -0.16060587266149184 2315 7 -0.43208529065023527 2315 8 -0.34445240310995473 2315 9 -0.5424057553028163 2315 10-11 -0.2973252040627372 2315 12-13 -0.2951575400139319 2315 14-15 -0.3174154989967164 2315 16-17 -0.2530501059022825 2315 18-19 -0.31449416220958426 2315 20-21 -0.15420974070236326 2315 22-23 -0.3122230411397666 2315 24-25 -0.4276998491217583 2315 26-27 -0.3886355357514759 2315 28-29 -0.4280100121785395 2315 30-31 -0.25890820890190724 2315 32-33 -0.36431494474391 2315 34-35 -0.3665713358803444 2315 36-37 -0.328113152260201 2315 38-39 -0.33150582349344404 2315 40-41 -0.4525923236249767 2315 42-43 -0.27486720704344947 2315 44-45 -0.12969006117523207 2315 46-47 -0.1909859862059946 2315 48-49 -0.07923391660443002 2315 50-51 0.18301847073804822 2315 52-53 0.3188515469942388 2315 54-55 0.29938347499738427 2315 56-57 0.5711687632858968 2315 58-59 0.7319125653033964 2315 60-61 0.6398850563139185 2315 62-63 0.6520700591705868 2315 64-65 0.8974853390669892 2315 66-67 0.8960329062513814 2315 68-69 1.0910306755242871 2315 70-71 1.0876753558754615 2315 72-73 1.070723250740027 2315 74-75 1.292367715446936 2315 76-77 1.256376145318157 2315 78-79 1.498308222349511 2315 80-81 1.5942471232060882 2315 82-83 1.859245817056653 2315 84-85 1.9931818524397436 2315 86-87 2.253584097550508 2315 88-89 2.5890092672209093 2315 90-91 2.542439840509111 2315 92-93 2.6356748118622875 2315 94-95 2.7543693241897635 2315 96-97 2.9032266821224084 2315 98-99 2.9632959900641147 2315 100-101 2.8751200882092114 2316 1 -0.6183683280358423 2316 2 -0.5732465878302513 2316 3 -0.5809268879816187 2316 4 -0.5034858870884733 2316 5 -0.585095108007117 2316 6 -0.6111625836737886 2316 7 -0.3861977205757725 2316 8 -0.38178491168623196 2316 9 -0.445223077591578 2316 10-11 -0.3561412892159197 2316 12-13 -0.4462500442871331 2316 14-15 -1.026684752292752 2316 16-17 -0.6962067141187731 2316 18-19 -0.5637010806064495 2316 20-21 -0.4166287591219415 2316 22-23 -0.49836099669851563 2316 24-25 -0.5806535850321524 2316 26-27 -0.40425452189793987 2316 28-29 -0.4208676459624954 2316 30-31 -0.29924898895473717 2316 32-33 -0.17458391714443167 2316 34-35 -0.28607295551194767 2316 36-37 -0.21131858848497131 2316 38-39 -0.2661958224176786 2316 40-41 -0.3722418616414913 2316 42-43 -0.13772161351719348 2316 44-45 -0.12422141389633623 2316 46-47 -0.00164417819510021 2316 48-49 0.2620124526715699 2316 50-51 0.3310584774317249 2316 52-53 0.3872463187516928 2316 54-55 0.3022578728649705 2316 56-57 0.566259367031968 2316 58-59 0.8166807069703665 2316 60-61 0.9571650613341838 2316 62-63 1.0638717050988014 2316 64-65 1.1065648384753182 2316 66-67 1.3644015308195812 2316 68-69 1.2915292650701922 2316 70-71 1.203563882189993 2316 72-73 1.5518939874034707 2316 74-75 1.9915523713798038 2316 76-77 2.0056205662550326 2316 78-79 1.887287854436149 2316 80-81 2.165349548469951 2316 82-83 2.270270069695279 2316 84-85 2.3245118628388504 2316 86-87 2.7742832584498487 2316 88-89 2.800539430618432 2316 90-91 3.0125899104341656 2316 92-93 3.3819840240400048 2316 94-95 3.535847701211317 2316 96-97 3.531043556173639 2316 98-99 3.6074617303732204 2316 100-101 3.3384227322129902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5494.0 3 1803.0 4 1904.0 5 2194.0 6 2595.0 7 3157.0 8 4187.0 9 5233.0 10 6765.0 11 8368.0 12 10898.0 13 13227.0 14 15327.0 15 19208.0 16 22818.0 17 27713.0 18 25853.0 19 22388.0 20 23973.0 21 26271.0 22 29101.0 23 32303.0 24 36411.0 25 41050.0 26 46339.0 27 52503.0 28 59608.0 29 66703.0 30 76303.0 31 87509.0 32 101496.0 33 119289.0 34 142843.0 35 172440.0 36 196872.0 37 202612.0 38 111224.0 39 8422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44572149767695 18.44938737019894 14.566288093357699 22.53860303876641 2 15.885105035594183 20.005077084334193 40.02822422151374 24.08159365855789 3 19.439304789915372 24.828471423369162 29.223108080883637 26.50911570583184 4 12.988485727685738 16.262537857217346 35.971495228366315 34.77748118673061 5 14.252989304196683 37.375080476541264 32.67339384113507 15.698536378126981 6 32.62026274489674 34.955948892633685 17.29242137475277 15.131366987716802 7 29.666053078639713 32.754355164839005 19.510516156362318 18.069075600158964 8 27.568167475701657 33.934159182274165 19.040818599288574 19.456854742735608 9 27.852741778921587 16.483102526950464 19.51887160191604 36.14528409221191 10-11 25.807139281442552 26.421941370524983 26.216243069009927 21.554676279022537 12-13 26.881572504106664 23.953981348186655 26.949320851478266 22.215125296228422 14-15 23.49819188829421 24.797291967529596 25.96024801795529 25.74426812622091 16-17 22.757661870071626 28.385027461880334 26.021264261782516 22.836046406265524 18-19 22.593412520071215 26.205617128965354 29.298774588006154 21.902195762957277 20-21 23.556665643020438 25.383068891391137 29.12300815504828 21.937257310540144 22-23 23.099343055572234 25.484819965345828 29.07484056787425 22.34099641120769 24-25 22.785592540345533 25.653673954630847 29.35630885358921 22.204424651434408 26-27 22.540201660071606 26.114992922651474 29.103995391874903 22.240810025402016 28-29 22.249012669063703 26.1073283594638 29.57876427767338 22.064894693799115 30-31 22.3357926044981 25.737290004738746 29.45166478499848 22.475252605764677 32-33 22.2274842448673 25.89107121800387 29.242094046022892 22.63935049110594 34-35 22.976452772342952 25.904898329955977 28.866262184683112 22.25238671301796 36-37 22.992652415498956 25.592543835346852 28.857072435626407 22.557731313527782 38-39 22.481427069195476 25.85783728417778 29.04462224919891 22.61611339742783 40-41 22.65057649884651 25.822405004665537 29.07594523185906 22.451073264628896 42-43 22.76976149473127 25.678848930287906 28.762305305302572 22.789084269678252 44-45 22.47472239110953 26.10025684211905 28.587575740044773 22.837445026726645 46-47 22.766590188549895 26.31309342292744 28.08691814840175 22.833398240120907 48-49 22.978409974426437 26.322103543593634 27.95388844512136 22.74559803685857 50-51 22.683075650184005 26.59864246306123 27.700542872876493 23.01773901387827 52-53 23.072544398300394 26.60320563355838 27.58126305902591 22.74298690911532 54-55 22.50875413966018 26.400007422462558 27.815924020180365 23.275314417696897 56-57 22.781911021023873 26.81632572867514 27.7007342692073 22.70102898109369 58-59 23.002628450796777 26.205970993167995 27.737769462597516 23.053631093437716 60-61 22.86589625535129 26.277187616658697 28.137968899746106 22.718947228243902 62-63 22.76096931361888 26.3437895245255 28.00992180710053 22.885319354755083 64-65 22.617829364709017 26.24048504377412 27.948251518490686 23.193434073026182 66-67 22.67023221546102 26.54173602204819 27.68433978224684 23.103691980243948 68-69 22.47688033442843 26.321339132727033 27.987941670957817 23.213838861886725 70-71 22.48771444626109 26.553416062111264 27.771369031098477 23.18750046052917 72-73 22.724489077449324 26.485745802556266 27.53471784434158 23.255047275652835 74-75 22.469599056861224 26.745079631913892 27.491703871890316 23.293617439334565 76-77 22.590273002150397 26.61413476547576 27.47276527927869 23.322826953095152 78-79 22.784487160000687 26.597642363988676 27.32607051841632 23.291799957594314 80-81 22.571038817261783 26.998066081770332 27.33010949955807 23.10078560140982 82-83 22.82825024621992 26.879192185802502 27.134540459070184 23.158017108907387 84-85 22.726664956639905 26.4513215414254 27.143527639671895 23.678485862262804 86-87 22.913272274059995 26.214102209021366 27.420980177338393 23.451645339580242 88-89 22.85911958439602 26.297595703133 27.297305042350505 23.54597967012048 90-91 22.72197385670509 26.456500217947227 27.386044444856893 23.43548148049079 92-93 22.991394468367652 26.135921715397142 27.236249720612914 23.636434095622295 94-95 22.778718625092285 26.021061939898278 27.3386545413358 23.86156489367364 96-97 23.057132267894872 25.94922510175776 27.109730263470315 23.88391236687705 98-99 22.94342664753061 26.05292653113689 26.790505219705775 24.213141601626727 100-101 23.335937457318774 25.54798599236245 27.169544861043583 23.946531689275197 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1519.0 1 1003.0 2 762.0 3 1036.0 4 820.5 5 687.0 6 1241.5 7 2927.0 8 4669.5 9 4941.5 10 3913.5 11 2934.0 12 2768.0 13 2883.0 14 3075.5 15 3178.5 16 3189.5 17 3390.5 18 3550.0 19 3666.0 20 3913.5 21 4346.5 22 4893.5 23 5498.0 24 6485.5 25 7707.5 26 9246.0 27 11086.0 28 12471.0 29 14099.5 30 16263.0 31 18575.0 32 21598.0 33 24846.5 34 28090.0 35 30542.0 36 33285.5 37 36761.0 38 39143.0 39 40758.0 40 43392.5 41 48697.0 42 54431.0 43 58450.5 44 61749.0 45 65411.5 46 66648.0 47 67868.5 48 73360.0 49 76056.0 50 73354.5 51 69764.5 52 66972.5 53 66330.0 54 65820.5 55 61817.5 56 56523.0 57 52136.5 58 47633.5 59 42468.5 60 36743.5 61 30900.5 62 25666.5 63 20634.0 64 16750.0 65 13924.0 66 10984.5 67 8880.5 68 6762.0 69 4582.0 70 3507.0 71 2848.0 72 2398.5 73 2148.0 74 1673.5 75 1306.0 76 971.5 77 646.5 78 438.0 79 294.5 80 172.0 81 89.5 82 58.5 83 43.0 84 24.5 85 18.0 86 13.5 87 10.0 88 7.0 89 4.5 90 4.5 91 3.0 92 1.5 93 0.5 94 1.0 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28820063697743514 2 0.035145088091927325 3 0.04283989775180582 4 0.046441723550046825 5 0.06139475792456249 6 0.061231038570096985 7 0.030342653694272657 8 0.03230728594785866 9 0.03874691389016833 10-11 0.032471005302324156 12-13 0.03410819884697916 14-15 0.028405307999764245 16-17 0.022374978443618326 18-19 0.02671354133695408 20-21 0.03910163915817691 22-23 0.03219813971154833 24-25 0.05487327030502007 26-27 0.046741875699900244 28-29 0.03809203647230633 30-31 0.03798289023599599 32-33 0.033835333256203325 34-35 0.032525578420479324 36-37 0.031515975734608744 38-39 0.02016476715833408 40-41 0.019291597267851412 42-43 0.020137480599256498 44-45 0.008185967723274998 46-47 0.015798917705920748 48-49 0.013015688680007248 50-51 0.006275908587844166 52-53 0.005375452138283916 54-55 0.006985359123861332 56-57 0.005784750524447665 58-59 0.007967675250654331 60-61 0.007421944069102665 62-63 0.004174843538870249 64-65 0.008567979550361165 66-67 0.0029469483803789996 68-69 0.007831242455266415 70-71 0.014871174697282913 72-73 0.015116753728981164 74-75 0.012224378466757332 76-77 0.010041453740550665 78-79 0.005593744610904582 80-81 0.006194048910611415 82-83 0.010941910190110916 84-85 0.004038410743482332 86-87 0.0017190532218877496 88-89 0.020273913394644412 90-91 0.029387624126557245 92-93 0.014652882224662248 94-95 0.023275434893178577 96-97 0.019482603181394498 98-99 0.014216297279420913 100-101 0.10772733523829899 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.60983182177782 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84297208566943 36.8671411794818 2 12.993358995901714 12.632099911815322 3 4.445051226139817 6.482195776255314 4 2.095358492004506 4.07420095610692 5 1.1194523494292095 2.7208195219123965 6 0.7111670288056376 2.0741825800461275 7 0.46930130483144306 1.596886025111813 8 0.3372764792066186 1.3115962345340055 9 0.2450771138600848 1.0721841559294871 >10 1.4910923140647399 13.717665116285637 >50 0.1468414518956324 4.932410813288636 >100 0.09703643635360787 8.671188429900065 >500 0.004312442072193555 1.4126107201767313 >1k 0.0013618238122716489 1.0007486653932942 >5k 2.2697063537860813E-4 0.8061816325482388 >10k+ 1.1348531768930406E-4 0.6278882812141818 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11382 0.6211512308421069 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8346 0.45546724412302086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6268 0.3420643045965846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.1089825169558678 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1919 0.1047258137397648 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1869 0.10199715783200647 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.7286559077583327E-5 0.0 22-23 0.0 0.0 0.0 3.001521498534166E-4 0.0 24-25 0.0 0.0 0.0 9.004564495602498E-4 0.0 26-27 0.0 0.0 0.0 0.0015280473083446665 0.0 28-29 0.0 0.0 0.0 0.00401112418440475 0.0 30-31 0.0 0.0 0.0 0.013424987066170997 0.0 32-33 0.0 0.0 0.0 0.031215823584755324 0.0 34-35 0.0 0.0 0.0 0.057028908472149156 0.0 36-37 0.0 0.0 0.0 0.1001689583738084 0.0 38-39 0.0 0.0 0.0 0.16573855983724112 0.0 40-41 0.0 0.0 0.0 0.23529199892600103 0.0 42-43 0.0 0.0 0.0 0.30929314714440703 0.0 44-45 0.0 0.0 0.0 0.39797446414655285 0.0 46-47 0.0 0.0 0.0 0.5085668880879981 0.0 48-49 0.0 0.0 0.0 0.631356403937123 0.0 50-51 0.0 0.0 0.0 0.7594122256882216 0.0 52-53 0.0 0.0 0.0 0.8838662216410791 0.0 54-55 0.0 5.4573118155166655E-5 0.0 1.0195349933748235 0.0 56-57 0.0 5.4573118155166655E-5 0.0 1.1874291913791937 0.0 58-59 0.0 5.4573118155166655E-5 0.0 1.3710131608531744 0.0 60-61 0.0 5.4573118155166655E-5 0.0 1.5793187528514454 0.0 62-63 0.0 5.4573118155166655E-5 0.0 1.787078613668165 2.7286559077583327E-5 64-65 0.0 5.4573118155166655E-5 0.0 1.9929284153494535 5.4573118155166655E-5 66-67 0.0 5.4573118155166655E-5 0.0 2.2309217836241353 5.4573118155166655E-5 68-69 0.0 5.4573118155166655E-5 0.0 2.5009222856968223 5.4573118155166655E-5 70-71 0.0 5.4573118155166655E-5 0.0 2.802575196299506 5.4573118155166655E-5 72-73 0.0 5.4573118155166655E-5 0.0 3.098825368204828 5.4573118155166655E-5 74-75 0.0 5.4573118155166655E-5 0.0 3.3986773659083913 5.4573118155166655E-5 76-77 0.0 5.4573118155166655E-5 0.0 3.7230872667817794 5.4573118155166655E-5 78-79 0.0 5.4573118155166655E-5 0.0 4.078849424035311 5.4573118155166655E-5 80-81 0.0 5.4573118155166655E-5 0.0 4.472567184965761 5.4573118155166655E-5 82-83 0.0 5.4573118155166655E-5 0.0 4.873597743729003 5.4573118155166655E-5 84-85 0.0 5.4573118155166655E-5 0.0 5.263331667034126 5.4573118155166655E-5 86-87 0.0 5.4573118155166655E-5 0.0 5.670037830085505 5.4573118155166655E-5 88-89 0.0 5.4573118155166655E-5 0.0 6.112734964560217 5.4573118155166655E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5680 0.0 46.551346 1 GTATCAA 9685 0.0 39.79573 1 TATCAAC 12360 0.0 31.221678 2 ATCAACG 12615 0.0 30.477543 3 TCAACGC 12830 0.0 29.966812 4 CAACGCA 13080 0.0 29.321384 5 AACGCAG 13390 0.0 28.63942 6 GTACATG 11160 0.0 28.474049 1 TACATGG 11460 0.0 27.16285 2 ACATGGG 11915 0.0 25.44751 3 ACGCAGA 15220 0.0 25.064182 7 CGCAGAG 15465 0.0 24.605675 8 GAGTACT 8140 0.0 23.633923 12-13 GCAGAGT 16010 0.0 23.232683 9 TTAGGGC 740 0.0 23.120087 3 CATGGGG 5125 0.0 22.626358 4 AGTACTT 8355 0.0 22.258224 12-13 CATGGGA 6590 0.0 22.211811 4 GTACTTT 8825 0.0 21.935211 14-15 AGAGTAC 14645 0.0 20.483074 10-11 >>END_MODULE