FastQCFastQC Report
Thu 26 May 2016
SRR938329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832404
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103200.5631945793613199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85780.4681282075350196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.3641118443312719No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.11493098683478098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20350.11105629544576415No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG20240.1104559911460573No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC18580.10139685353229966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.044.441761
GTATCAA96400.039.6733361
TATCAAC127500.029.9961552
ATCAACG130350.029.2545343
TCAACGC131800.028.932694
CAACGCA134250.028.404685
AACGCAG138200.027.5963216
GTACATG109350.026.2312071
TACATGG112550.025.2318172
ACGCAGA154450.024.5698537
CGCAGAG156950.024.1179688
ACATGGG114200.023.6489623
AGTACTT88300.022.80598612-13
GCAGAGT165450.022.5631379
GAGTACT86200.022.48001512-13
GGATACG1100.002854035721.6220571
GTACTTT91650.021.19505514-15
CATGGGG47900.021.1433564
AGAGTAC150850.020.8113310-11
TATTGGG6700.020.5893632
Produced by FastQC (version 0.11.5)