##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832404 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7088076646853 31.0 31.0 34.0 30.0 34.0 2 31.939993582201303 31.0 31.0 34.0 30.0 34.0 3 31.99335572286461 33.0 31.0 34.0 30.0 34.0 4 35.69031720079197 37.0 35.0 37.0 33.0 37.0 5 35.56326989026437 37.0 35.0 37.0 33.0 37.0 6 35.61289104367814 37.0 35.0 37.0 33.0 37.0 7 35.54166111839965 37.0 35.0 37.0 33.0 37.0 8 35.531262210735186 37.0 35.0 37.0 33.0 37.0 9 37.05834412061969 39.0 37.0 39.0 33.0 39.0 10-11 36.99405780602967 39.0 37.0 39.0 33.0 39.0 12-13 36.8693320359484 39.0 37.0 39.0 32.5 39.0 14-15 37.84553488204566 40.0 37.0 41.0 32.5 41.0 16-17 37.77156265757988 39.5 37.0 41.0 32.0 41.0 18-19 37.793656584465 39.5 37.0 41.0 32.5 41.0 20-21 37.812205168729164 39.5 37.0 41.0 32.0 41.0 22-23 37.823835245939215 40.0 37.0 41.0 32.0 41.0 24-25 37.73312899338792 40.0 37.0 41.0 32.0 41.0 26-27 37.54400339663087 39.0 36.5 41.0 32.0 41.0 28-29 37.39122567948989 39.0 36.0 41.0 31.5 41.0 30-31 37.105728594785866 39.0 36.0 40.5 31.0 41.0 32-33 36.86233112348587 39.0 35.0 40.0 30.5 41.0 34-35 36.65489433552863 39.0 35.0 40.0 30.0 41.0 36-37 36.63477131680568 39.0 35.0 40.0 30.0 41.0 38-39 36.47700670812768 39.0 35.0 40.0 30.0 41.0 40-41 36.26396798959182 38.5 35.0 40.0 30.0 41.0 42-43 35.99263754062969 38.0 35.0 40.0 29.5 41.0 44-45 35.63833903440508 38.0 35.0 40.0 28.5 41.0 46-47 35.166059722637584 38.0 34.0 40.0 26.5 41.0 48-49 34.69411985566502 38.0 33.5 40.0 24.5 41.0 50-51 34.29113994512127 38.0 33.0 40.0 24.0 41.0 52-53 33.746684683072075 37.0 33.0 40.0 23.0 41.0 54-55 33.39225247270798 37.0 32.0 40.0 22.0 41.0 56-57 33.00148820893209 36.5 31.5 39.0 20.5 41.0 58-59 32.51806588503408 36.0 31.0 39.0 18.5 40.0 60-61 32.08572727411641 35.5 31.0 39.0 17.5 40.0 62-63 31.582368844425137 35.0 30.0 38.5 13.5 40.0 64-65 31.052968122750222 35.0 29.0 38.0 9.5 40.0 66-67 30.528448966494288 34.0 29.0 38.0 7.5 40.0 68-69 29.93553932429748 34.0 28.0 37.0 6.5 39.0 70-71 29.350313304271328 34.0 27.5 36.5 2.0 39.0 72-73 28.750853523567947 33.5 26.0 36.0 2.0 39.0 74-75 28.106124249892492 33.0 26.0 35.0 2.0 38.0 76-77 26.87338299850906 31.5 25.0 34.0 2.0 36.0 78-79 27.490009026393743 32.5 26.0 35.0 2.0 37.0 80-81 27.152533775302825 32.0 25.5 35.0 2.0 36.0 82-83 26.633175598830825 32.0 24.5 35.0 2.0 36.0 84-85 26.013233435421448 32.0 23.5 35.0 2.0 35.5 86-87 25.325796330940122 31.0 20.5 34.0 2.0 35.0 88-89 24.544802892811845 31.0 18.0 34.0 2.0 35.0 90-91 23.966649276033014 31.0 11.0 34.0 2.0 35.0 92-93 23.147036079379873 30.0 4.5 34.0 2.0 35.0 94-95 22.299102981656883 29.5 2.0 34.0 2.0 35.0 96-97 21.26663879799433 29.0 2.0 34.0 2.0 35.0 98-99 20.06324123937734 29.0 2.0 34.0 2.0 35.0 100-101 17.081288023820075 22.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20270595942718472 1101 2 0.2640495151540918 1101 3 0.21059563907151357 1101 4 0.2020024427469238 1101 5 0.19169620372978358 1101 6 0.24606945582434037 1101 7 0.21478356881851113 1101 8 0.21675557324139305 1101 9 0.25971526388175903 1101 10-11 0.33994340007588875 1101 12-13 0.3702170733887513 1101 14-15 0.5135432300710008 1101 16-17 0.4434861413811504 1101 18-19 0.5453065592918875 1101 20-21 0.4359390983561582 1101 22-23 0.4526062292781958 1101 24-25 0.4453542641077348 1101 26-27 0.5062015563220541 1101 28-29 0.3115713022668274 1101 30-31 0.44262553938285265 1101 32-33 0.4472023249907622 1101 34-35 0.2504605115451497 1101 36-37 0.5482684784246032 1101 38-39 0.6032419233598887 1101 40-41 0.8339214387368372 1101 42-43 0.7523963243485952 1101 44-45 0.6373229004075327 1101 46-47 0.7860221022048748 1101 48-49 0.8474259533514967 1101 50-51 1.0651020226103682 1101 52-53 0.9986221777136208 1101 54-55 1.3629531774016002 1101 56-57 1.5096472627126758 1101 58-59 1.4483977962927739 1101 60-61 1.497204307313659 1101 62-63 1.3948915049732626 1101 64-65 1.5509485981457658 1101 66-67 1.6992269736855121 1101 68-69 1.6143769235332606 1101 70-71 1.8568506710887753 1101 72-73 1.937282552866197 1101 74-75 2.0760984668717697 1101 76-77 1.9269620587472964 1101 78-79 2.0431048508182386 1101 80-81 2.4805327056761364 1101 82-83 2.5712612538941144 1101 84-85 2.5718610406752695 1101 86-87 2.6741409390721778 1101 88-89 2.92835554496385 1101 90-91 3.2994565665206075 1101 92-93 3.530339484533794 1101 94-95 3.6117132219542185 1101 96-97 3.9693041642682303 1101 98-99 4.091327871839031 1101 100-101 4.3787078681546205 1102 1 0.30121257871642726 1102 2 0.28410696558901094 1102 3 0.28001277183932416 1102 4 0.2125536950978386 1102 5 0.17548990377288476 1102 6 0.25113708130432144 1102 7 0.28357348536110294 1102 8 0.2998741204751525 1102 9 0.35294028788066356 1102 10-11 0.41699487772262245 1102 12-13 0.3305238369162993 1102 14-15 0.5361358707339505 1102 16-17 0.42308783918108617 1102 18-19 0.40390940071301884 1102 20-21 0.5774541086903753 1102 22-23 0.5578545811902771 1102 24-25 0.5022031959116688 1102 26-27 0.5369686702335841 1102 28-29 0.54217091363153 1102 30-31 0.49063007958346816 1102 32-33 0.38523709646243276 1102 34-35 0.4852628389489553 1102 36-37 0.641600487328688 1102 38-39 0.6529984753052673 1102 40-41 0.6557797864368666 1102 42-43 0.6272114348414988 1102 44-45 0.43750922862436425 1102 46-47 0.5862021551983645 1102 48-49 0.6964647421905426 1102 50-51 0.7349746141490456 1102 52-53 1.008505042252061 1102 54-55 0.8142886673710379 1102 56-57 1.0087950567727049 1102 58-59 1.100640224394013 1102 60-61 1.234163168786111 1102 62-63 1.2064344568872976 1102 64-65 1.2774974763194962 1102 66-67 1.2191886121738733 1102 68-69 1.4293023738800166 1102 70-71 1.5383136001303868 1102 72-73 1.4184804532070814 1102 74-75 1.6549461889350887 1102 76-77 1.714912466728645 1102 78-79 2.0933471968824193 1102 80-81 2.1685866089262724 1102 82-83 2.441083429370174 1102 84-85 2.5728206906831055 1102 86-87 2.51195534306677 1102 88-89 2.8725414127935665 1102 90-91 3.078253413358631 1102 92-93 3.255607298821463 1102 94-95 3.4131677575019808 1102 96-97 3.849046702568522 1102 98-99 4.173355754646021 1102 100-101 4.58724272288779 1103 1 0.3124837156383684 1103 2 0.31841675117893686 1103 3 0.35136136112120653 1103 4 0.26668120827487485 1103 5 0.1849269878912807 1103 6 0.2257229884330343 1103 7 0.29003589801105534 1103 8 0.23450110895567633 1103 9 0.388213905186106 1103 10-11 0.4039039979019705 1103 12-13 0.4110337465253977 1103 14-15 0.5029356990150973 1103 16-17 0.5691096856358016 1103 18-19 0.5021558411241926 1103 20-21 0.5878873708716839 1103 22-23 0.4986029295887562 1103 24-25 0.4993866787040133 1103 26-27 0.43258208815435495 1103 28-29 0.5233943806829799 1103 30-31 0.5769714244760209 1103 32-33 0.6479350501460459 1103 34-35 0.5671530581289943 1103 36-37 0.6523081717475847 1103 38-39 0.9161082859375256 1103 40-41 0.7567281157554788 1103 42-43 1.1021828150318242 1103 44-45 1.0208608468361007 1103 46-47 1.033794811832209 1103 48-49 1.080925079904297 1103 50-51 1.2770415606538492 1103 52-53 1.3242408694838161 1103 54-55 1.353471422743219 1103 56-57 1.4431488155077048 1103 58-59 1.7574234174107843 1103 60-61 1.6099032591770097 1103 62-63 1.6084566253148758 1103 64-65 1.5633685321519835 1103 66-67 1.6943308168532099 1103 68-69 1.8571054755518936 1103 70-71 1.7622369288527544 1103 72-73 1.780668616530793 1103 74-75 1.9139138783624539 1103 76-77 1.7145639609212076 1103 78-79 1.8657101497312816 1103 80-81 2.2857127328500475 1103 82-83 2.451817348614611 1103 84-85 2.756231188190796 1103 86-87 2.8400352519603764 1103 88-89 2.9443274976036022 1103 90-91 3.3825796255268195 1103 92-93 3.6576419884157794 1103 94-95 3.55815392459397 1103 96-97 4.0038587061936965 1103 98-99 4.374800309727231 1103 100-101 4.702781043620458 1104 1 0.2884876328051753 1104 2 0.36678688908153845 1104 3 0.24428182659973885 1104 4 0.19729295061235064 1104 5 0.18721486529082654 1104 6 0.19206112468629755 1104 7 0.25183149520719184 1104 8 0.2524410457312314 1104 9 0.27269356340757867 1104 10-11 0.3127972264720924 1104 12-13 0.3788213490360519 1104 14-15 0.5245098961104375 1104 16-17 0.4893964863871716 1104 18-19 0.5876634692911367 1104 20-21 0.5782379114865179 1104 22-23 0.4636616870909265 1104 24-25 0.6313118745623498 1104 26-27 0.5509664286500708 1104 28-29 0.5952248544081797 1104 30-31 0.6567524869216186 1104 32-33 0.6609241233123058 1104 34-35 0.6943017730233976 1104 36-37 0.6412490477268804 1104 38-39 0.9685986075575386 1104 40-41 0.9078832218167747 1104 42-43 0.9033705641498813 1104 44-45 1.0908292346127055 1104 46-47 0.9433981700582592 1104 48-49 0.8908342520305581 1104 50-51 0.8898044231635822 1104 52-53 1.1581066091640224 1104 54-55 1.2091693542042492 1104 56-57 1.3732442821316084 1104 58-59 1.4557998548506461 1104 60-61 1.5386053826937527 1104 62-63 1.6787291089360785 1104 64-65 1.8340029528236244 1104 66-67 1.8091788261994282 1104 68-69 1.913614901950389 1104 70-71 1.760340481791772 1104 72-73 2.050801364961636 1104 74-75 2.186373733129482 1104 76-77 2.1958182560602566 1104 78-79 2.567548755586415 1104 80-81 2.7169472703494755 1104 82-83 2.875744709223035 1104 84-85 3.090614921640192 1104 86-87 3.167258988616812 1104 88-89 3.1095239858979156 1104 90-91 3.4003887037327125 1104 92-93 3.706446794390029 1104 94-95 3.970893879695449 1104 96-97 4.055178745594439 1104 98-99 4.475278788217171 1104 100-101 4.81809836849332 1105 1 0.2874052725784715 1105 2 0.36530658225925805 1105 3 0.28956678634444444 1105 4 0.20185434532249502 1105 5 0.09646205390716034 1105 6 0.172751992684141 1105 7 0.16133108008892805 1105 8 0.24894756524124517 1105 9 0.3591902541818115 1105 10-11 0.3104979946180535 1105 12-13 0.32677184229903133 1105 14-15 0.43725833335235365 1105 16-17 0.4440495178407602 1105 18-19 0.4531190881607827 1105 20-21 0.3798244645984852 1105 22-23 0.3619374062976348 1105 24-25 0.23160695468862258 1105 26-27 0.43522665955721607 1105 28-29 0.4041236762136933 1105 30-31 0.489823293613874 1105 32-33 0.6892925368191598 1105 34-35 0.6924409638398785 1105 36-37 0.7590112577369226 1105 38-39 0.7296220008209033 1105 40-41 0.7584985671826345 1105 42-43 0.6631417884680246 1105 44-45 0.734285575237557 1105 46-47 0.8465945261757497 1105 48-49 0.9351297966095728 1105 50-51 0.9569809430061227 1105 52-53 1.08123354813155 1105 54-55 1.1469197402942157 1105 56-57 1.193031713233701 1105 58-59 1.3046942174737595 1105 60-61 1.3675064722497297 1105 62-63 1.5137679795963486 1105 64-65 1.4849105708842956 1105 66-67 1.7109503427085002 1105 68-69 1.8792231851270067 1105 70-71 1.8117557270713327 1105 72-73 1.684567433418266 1105 74-75 1.9686481560115219 1105 76-77 1.9349746201803022 1105 78-79 2.2533320316899434 1105 80-81 2.66780536313863 1105 82-83 2.576579770171552 1105 84-85 2.9823573662424856 1105 86-87 3.105983935016674 1105 88-89 3.3479835346264757 1105 90-91 3.322315533891828 1105 92-93 3.5495977791443174 1105 94-95 3.6552236967435903 1105 96-97 4.135828892207776 1105 98-99 4.422264124702824 1105 100-101 4.605180585895365 1106 1 0.08141587990488475 1106 2 0.1621126330319882 1106 3 0.16251170908510204 1106 4 0.07656114350535859 1106 5 0.016467388451694376 1106 6 0.11020500455651216 1106 7 0.1609656965824513 1106 8 0.049569783018014846 1106 9 0.0031140521807557775 1106 10-11 0.16861523380541854 1106 12-13 0.1816371136215693 1106 14-15 -0.029436427124686304 1106 16-17 0.009633124391861259 1106 18-19 0.12800565393568775 1106 20-21 0.35267253474775373 1106 22-23 0.2935336239087931 1106 24-25 0.18090281940545339 1106 26-27 0.14580643873659227 1106 28-29 0.4613027789006665 1106 30-31 0.3563354716521232 1106 32-33 0.47872382572656136 1106 34-35 0.36320200275335424 1106 36-37 0.4367637747701636 1106 38-39 0.3955808565064416 1106 40-41 0.3320218179535033 1106 42-43 0.5131632004334108 1106 44-45 -0.03907047910156081 1106 46-47 -0.3367979326038153 1106 48-49 0.27007934389749266 1106 50-51 0.22733727254433944 1106 52-53 0.4383945592791676 1106 54-55 0.3285436281222971 1106 56-57 0.40066492175727575 1106 58-59 0.750765306292017 1106 60-61 0.5420567594870747 1106 62-63 0.7874280647140388 1106 64-65 0.22354303087725924 1106 66-67 0.45878079273690275 1106 68-69 0.5946185385528828 1106 70-71 0.8314003513015926 1106 72-73 1.236706593976301 1106 74-75 1.4161574128947123 1106 76-77 1.328088329877776 1106 78-79 1.7330367132720141 1106 80-81 1.7757723632963156 1106 82-83 2.048772090340421 1106 84-85 1.842351534317256 1106 86-87 2.1997429278371747 1106 88-89 2.5193841996408572 1106 90-91 2.155495143797797 1106 92-93 1.8510539294341761 1106 94-95 2.642253812051191 1106 96-97 3.2395499829800265 1106 98-99 3.4074708751972658 1106 100-101 3.6239673650793804 1107 1 0.07849883754625964 1107 2 0.10855420288209672 1107 3 0.17200909394529162 1107 4 0.12555838995982782 1107 5 0.04303697145925156 1107 6 0.05132753166703452 1107 7 -0.006540577540746995 1107 8 0.10607924991584383 1107 9 0.25469978252323244 1107 10-11 0.1865143589625049 1107 12-13 0.21499963896222596 1107 14-15 0.3207022753796238 1107 16-17 0.4438421100262673 1107 18-19 0.2958283996355391 1107 20-21 0.2998635999430519 1107 22-23 0.3776704119354335 1107 24-25 0.3478553357187124 1107 26-27 0.2805958111192268 1107 28-29 0.34097248833497673 1107 30-31 0.22839340564580368 1107 32-33 0.3350109261508436 1107 34-35 0.52564108939427 1107 36-37 0.4768401919989529 1107 38-39 0.5721079195476833 1107 40-41 0.5614928490680882 1107 42-43 0.5624361791567054 1107 44-45 0.5692775986236427 1107 46-47 0.6223739964482746 1107 48-49 0.7504197950084404 1107 50-51 0.7619207630342686 1107 52-53 0.867237827337874 1107 54-55 0.6878690445400792 1107 56-57 0.803077147434692 1107 58-59 0.6391833099364348 1107 60-61 0.9911582554020839 1107 62-63 1.071627229747083 1107 64-65 1.1433530993678573 1107 66-67 1.31052802340826 1107 68-69 1.33844707301159 1107 70-71 1.5135600957932844 1107 72-73 1.7029856436658477 1107 74-75 1.5800069968951185 1107 76-77 1.6668872055807995 1107 78-79 1.8562492424168084 1107 80-81 2.09372417975683 1107 82-83 2.2577722937671645 1107 84-85 2.47036523135818 1107 86-87 2.6412604373758377 1107 88-89 2.6218403212479586 1107 90-91 2.776028186613999 1107 92-93 2.882700277708313 1107 94-95 3.1712962170840875 1107 96-97 3.6115234705939265 1107 98-99 3.7463842796166453 1107 100-101 4.043011480623612 1108 1 0.18457450847724033 1108 2 0.176153324068963 1108 3 0.09861200050739427 1108 4 0.15801752541042902 1108 5 0.048577883031938995 1108 6 0.12447618536398153 1108 7 0.11500392870159715 1108 8 0.14134254118074097 1108 9 0.23370623255183887 1108 10-11 0.25662138920632316 1108 12-13 0.2892946160906149 1108 14-15 0.40229631282583966 1108 16-17 0.3799792508532036 1108 18-19 0.3455701633748305 1108 20-21 0.4164199795673369 1108 22-23 0.3442231341923403 1108 24-25 0.36334128228457274 1108 26-27 0.3432598375149638 1108 28-29 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2.1865887058804816 2316 90-91 2.676014007839914 2316 92-93 2.727590565826791 2316 94-95 3.258612724938839 2316 96-97 3.439763682175961 2316 98-99 3.8479212297912753 2316 100-101 4.184368215685982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 47.0 5 105.0 6 242.0 7 498.0 8 1102.0 9 1829.0 10 2892.0 11 4535.0 12 6388.0 13 9072.0 14 11872.0 15 15758.0 16 20027.0 17 25681.0 18 23972.0 19 19411.0 20 19600.0 21 20844.0 22 22893.0 23 25669.0 24 29193.0 25 34019.0 26 40479.0 27 47176.0 28 55714.0 29 65382.0 30 76686.0 31 89310.0 32 105619.0 33 126062.0 34 154183.0 35 190609.0 36 226971.0 37 238196.0 38 115365.0 39 4990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85860074219603 19.43205631958088 15.331423270028377 22.377919668194718 2 16.368456799425203 20.910615659785236 38.78175099749071 23.93917654329885 3 19.509703389159906 25.064928473625585 29.791841414624855 25.63352672258965 4 13.494022060637285 17.111783209379592 35.54418130499606 33.85001342498707 5 14.158220262411216 37.67760938990321 32.83128350746929 15.332886840216284 6 32.4376065540132 35.5440175856416 16.904514506626267 15.11386135371894 7 29.39837503083381 32.935640830297245 19.468086731965222 18.197897406903717 8 27.477346698653793 34.4809878170971 18.85872329464463 19.182942189604475 9 27.258890506678657 16.498163068842896 19.568664988725192 36.67428143575325 10-11 25.81125668793563 26.637084398418686 26.142651947932876 21.409006965712802 12-13 26.74000929925933 23.999674744215795 27.070340383452557 22.189975573072314 14-15 23.194748537986165 25.053890954178225 25.98796444452206 25.76339606331355 16-17 22.52650070617615 28.59265751439093 26.15842357907972 22.722418200353196 18-19 22.373886981255225 26.306153009925758 29.423178513035335 21.89678149578368 20-21 23.35874075804244 25.446953837690817 29.285654255284317 21.90865114898243 22-23 22.91828112141209 25.549906025090536 29.299761406327423 22.232051447169948 24-25 22.729017845326677 25.792403286537297 29.39402183329973 22.0845570348363 26-27 22.41983209816169 26.305512794307834 29.204811806998304 22.069843300532174 28-29 22.154885058098543 26.202218506399245 29.65896167002473 21.983934765477482 30-31 22.268069705152357 25.83666047443686 29.533307065472464 22.36196275493832 32-33 22.189681395809536 25.9010182825902 29.36535656262637 22.543943758973892 34-35 22.84013233679567 25.99329692242993 28.984001526081336 22.182569214693064 36-37 22.945564418753207 25.54205413405728 29.104348303020394 22.408033144169114 38-39 22.426691212769796 25.910183858353776 29.189686597822234 22.473438331054197 40-41 22.53964156407214 25.792640264062427 29.32485179780101 22.342866374064425 42-43 22.7274401749692 25.767898391249354 28.865633642549277 22.639027791232166 44-45 22.48063496204215 26.10472878850598 28.651360553034976 22.76327569641689 46-47 22.76294495180783 26.290170259989132 28.19561848686711 22.751266301335924 48-49 22.926412516017212 26.330083322236796 28.01579236893174 22.727711792814247 50-51 22.682825403131623 26.560927612033154 27.755833320599603 23.000413664235616 52-53 23.120529097295137 26.635911076378356 27.498630214734305 22.744929611592205 54-55 22.5600358872825 26.4174821709623 27.73184843517041 23.290633506584793 56-57 22.806897387257397 26.84661788557545 27.63408069399543 22.712404033171723 58-59 23.075042403312807 26.22882290149989 27.684151529902795 23.011983165284512 60-61 22.89773434242667 26.359580092599664 28.023323459237155 22.719362105736508 62-63 22.669946147247007 26.469981510627573 27.861350444552617 22.998721897572803 64-65 22.48718077454535 26.34727385445568 27.86710790851799 23.29843746248098 66-67 22.60873978526546 26.620605085565284 27.62333265298772 23.147322476181536 68-69 22.43273568296844 26.436778472799034 27.7743426919261 23.35614315230643 70-71 22.40671271182556 26.641750400020957 27.633835114963727 23.317701773189757 72-73 22.694319237002112 26.557633185049035 27.372055336065447 23.375992241883406 74-75 22.520443090060926 26.807652679212662 27.137901903728658 23.534002326997758 76-77 22.47986797671256 26.70006177676975 27.439773106803962 23.380297139713733 78-79 22.591797442048804 26.659159224712454 27.32462382749656 23.424419505742183 80-81 22.29021126902761 27.07638548943655 27.354353818455873 23.279049423079968 82-83 22.608157272239335 26.881648252186558 27.20556955821893 23.30462491735518 84-85 22.50603737853613 26.525034996329822 27.11160895796893 23.85731866716512 86-87 22.620997977500373 26.300872196142077 27.41709443315983 23.66103539319772 88-89 22.390756184432085 26.455690572518552 27.238514879501157 23.915038363548202 90-91 22.37612847684107 26.52521225665353 27.460055637643137 23.638603628862267 92-93 22.56461471294477 26.222440515171357 27.237038856144945 23.97590591573892 94-95 22.275218783630685 26.13528457698193 27.272833938367302 24.316662701020082 96-97 22.540116699155863 26.143743410295983 27.065074077550584 24.25106581299757 98-99 22.395770801635447 26.174495362376422 26.889048484941092 24.54068535104704 100-101 22.405907244839266 25.922918324334177 27.244822892893122 24.426351537933435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1577.0 1 1051.5 2 782.0 3 998.0 4 802.5 5 741.5 6 1290.5 7 2892.0 8 4353.0 9 4595.0 10 3771.0 11 2795.5 12 2639.0 13 2769.0 14 2958.5 15 3130.0 16 3195.5 17 3374.5 18 3581.0 19 3790.5 20 4023.0 21 4358.5 22 4977.5 23 5672.0 24 6430.0 25 7625.5 26 9321.0 27 11195.0 28 12697.0 29 14383.0 30 16501.5 31 18949.0 32 22158.5 33 25149.5 34 28132.5 35 30633.5 36 33497.0 37 37020.5 38 39490.0 39 40839.0 40 43624.5 41 49404.0 42 55056.5 43 59158.0 44 62359.0 45 65879.5 46 66809.0 47 68079.5 48 74202.0 49 76738.5 50 73978.5 51 70485.0 52 67062.5 53 66280.5 54 66629.5 55 62823.5 56 56898.5 57 52293.0 58 47638.0 59 41936.0 60 36105.0 61 29936.0 62 24292.5 63 19530.0 64 15864.0 65 13165.5 66 10106.5 67 8146.0 68 6188.0 69 3992.5 70 2911.0 71 2391.0 72 2150.0 73 1895.0 74 1523.0 75 1276.0 76 929.0 77 532.5 78 317.5 79 194.5 80 108.5 81 67.0 82 39.0 83 21.5 84 10.0 85 7.5 86 5.5 87 4.0 88 3.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.1829247262066662E-4 2 0.04409507946937466 3 1.6371935446549997E-4 4 0.0 5 0.10052368364181698 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010641758040257497 26-27 0.011051056426421247 28-29 0.0 30-31 0.0 32-33 2.7286559077583327E-4 34-35 0.014216297279420913 36-37 0.002483076876060083 38-39 0.011214775780886747 40-41 0.006003042997068332 42-43 0.004447709129646082 44-45 0.002319357521594583 46-47 2.7286559077583327E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0914623631033331E-4 68-69 3.2743870893099994E-4 70-71 0.0 72-73 2.7286559077583327E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.4573118155166655E-5 82-83 7.913102132499165E-4 84-85 0.003765545152706499 86-87 9.82316126793E-4 88-89 2.7286559077583327E-5 90-91 6.275908587844165E-4 92-93 2.1829247262066662E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 5.457311815516665E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.80323626298299 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59229399903651 34.911607594347934 2 13.604256043061538 12.734464195310464 3 4.68907073915608 6.58391056975687 4 2.1761537108394386 4.074041450919416 5 1.2254249231561376 2.867692610051224 6 0.7338086632530395 2.060677214283344 7 0.4849070428978231 1.5886653226031868 8 0.34326025246640707 1.2852552556701151 9 0.27405417723055603 1.1543960165201215 >10 1.5998027042068768 14.270242283050507 >50 0.16339757455354653 5.280244999562255 >100 0.10688996324433589 9.21692826815541 >500 0.004922257714080416 1.5269646121436458 >1k 0.0014063593468801186 1.04234889448113 >5k 2.3439322448001978E-4 0.8364900616132839 >10k+ 1.1719661224000989E-4 0.5660706515310878 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10320 0.5631945793613199 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8578 0.4681282075350196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6672 0.3641118443312719 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2106 0.11493098683478098 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2035 0.11105629544576415 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2024 0.1104559911460573 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1858 0.10139685353229966 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.7286559077583327E-5 0.0 22-23 0.0 0.0 0.0 3.001521498534166E-4 0.0 24-25 0.0 0.0 0.0 9.550295677154164E-4 0.0 26-27 0.0 0.0 0.0 0.0015826204264998331 0.0 28-29 0.0 0.0 0.0 0.004065697302559916 0.0 30-31 0.0 0.0 0.0 0.013288554270783081 0.0 32-33 0.0 0.0 0.0 0.031352256380143245 0.0 34-35 0.0 0.0 0.0 0.05721991438569224 0.0 36-37 0.0 0.0 0.0 0.10079654923259282 0.0 38-39 0.0 0.0 0.0 0.16655715660956863 0.0 40-41 0.0 0.0 0.0 0.23641074784818195 0.0 42-43 0.0 2.7286559077583327E-5 0.0 0.3109576272481396 0.0 44-45 0.0 5.4573118155166655E-5 0.0 0.40007552919552675 0.0 46-47 0.0 5.4573118155166655E-5 0.0 0.5116775558228426 0.0 48-49 0.0 5.4573118155166655E-5 0.0 0.6341396329630365 0.0 50-51 0.0 5.4573118155166655E-5 0.0 0.7617861563279713 0.0 52-53 0.0 5.4573118155166655E-5 0.0 0.8857217076583548 0.0 54-55 0.0 5.4573118155166655E-5 0.0 1.0201898707926855 0.0 56-57 0.0 5.4573118155166655E-5 0.0 1.1872927585838058 0.0 58-59 0.0 5.4573118155166655E-5 0.0 1.3692941076312866 0.0 60-61 0.0 5.4573118155166655E-5 0.0 1.5755804942578164 0.0 62-63 0.0 5.4573118155166655E-5 0.0 1.783394928192691 0.0 64-65 0.0 8.185967723274999E-5 0.0 1.9886989986924282 0.0 66-67 0.0 1.3643279538791663E-4 0.0 2.2259283433129378 0.0 68-69 0.0 1.6371935446549997E-4 0.0 2.494782809904366 0.0 70-71 0.0 1.6371935446549997E-4 0.0 2.7953442581439463 0.0 72-73 0.0 1.6371935446549997E-4 0.0 3.090584827363398 0.0 74-75 0.0 1.6371935446549997E-4 0.0 3.3918011530208405 0.0 76-77 0.0 1.6371935446549997E-4 0.0 3.7192398619518405 0.0 78-79 0.0 1.6371935446549997E-4 0.0 4.078330979412836 0.0 80-81 0.0 1.6371935446549997E-4 0.0 4.4753504139916735 2.7286559077583327E-5 82-83 0.0 1.6371935446549997E-4 0.0 4.882029290483976 5.4573118155166655E-5 84-85 0.0 1.6371935446549997E-4 0.0 5.2753923261464175 5.4573118155166655E-5 86-87 0.0 1.6371935446549997E-4 0.0 5.689438573589667 5.4573118155166655E-5 88-89 0.0 1.6371935446549997E-4 0.0 6.141658717182455 5.4573118155166655E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5705 0.0 44.44176 1 GTATCAA 9640 0.0 39.673336 1 TATCAAC 12750 0.0 29.996155 2 ATCAACG 13035 0.0 29.254534 3 TCAACGC 13180 0.0 28.93269 4 CAACGCA 13425 0.0 28.40468 5 AACGCAG 13820 0.0 27.596321 6 GTACATG 10935 0.0 26.231207 1 TACATGG 11255 0.0 25.231817 2 ACGCAGA 15445 0.0 24.569853 7 CGCAGAG 15695 0.0 24.117968 8 ACATGGG 11420 0.0 23.648962 3 AGTACTT 8830 0.0 22.805986 12-13 GCAGAGT 16545 0.0 22.563137 9 GAGTACT 8620 0.0 22.480015 12-13 GGATACG 110 0.0028540357 21.622057 1 GTACTTT 9165 0.0 21.195055 14-15 CATGGGG 4790 0.0 21.143356 4 AGAGTAC 15085 0.0 20.81133 10-11 TATTGGG 670 0.0 20.589363 2 >>END_MODULE