FastQCFastQC Report
Thu 26 May 2016
SRR938328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832672
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107380.5859204483944754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.4347750170243229No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60170.3283184334130712No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG19160.10454680379249533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.10421941296642279No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG18970.10351006617659898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18730.10220050287230885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51900.047.9696351
GTATCAA91250.039.566381
TATCAAC119400.030.0950832
ATCAACG120400.029.88463
TCAACGC122550.029.2439564
CAACGCA125100.028.4198885
AACGCAG129650.027.413526
GTACATG111650.027.168251
TACATGG113550.026.4968742
ACATGGG116900.024.7210643
ACGCAGA146050.024.1035637
CGCAGAG148000.023.6575838
GATGTGC10750.023.4224348
GCAGAGT153200.022.2945739
GAGTACT77500.022.21712912-13
AGTACTT79250.022.05617512-13
CATGGGG49900.021.0508584
CATGGGA63900.021.050564
AGAGTAC140900.020.59793510-11
GTACTTT84600.020.4095514-15
Produced by FastQC (version 0.11.5)