##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938328_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832672 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25816676415638 31.0 31.0 34.0 28.0 34.0 2 31.432836317682597 31.0 31.0 34.0 28.0 34.0 3 31.48010827905921 33.0 31.0 34.0 28.0 34.0 4 35.16168687031831 37.0 35.0 37.0 32.0 37.0 5 35.1608525693632 37.0 35.0 37.0 32.0 37.0 6 35.15418470953886 37.0 35.0 37.0 32.0 37.0 7 35.07843411150495 37.0 35.0 37.0 32.0 37.0 8 35.044697032529555 37.0 35.0 37.0 32.0 37.0 9 36.533715252920324 39.0 37.0 39.0 32.0 39.0 10-11 36.378054010755875 39.0 37.0 39.0 32.0 39.0 12-13 36.192716972813464 38.5 35.0 39.0 32.0 39.0 14-15 37.35494049126085 39.5 37.0 41.0 32.0 41.0 16-17 37.3033112308149 39.0 37.0 41.0 32.0 41.0 18-19 37.31869314312654 40.0 37.0 41.0 32.0 41.0 20-21 37.26830414826003 40.0 37.0 41.0 31.5 41.0 22-23 37.11609033149412 39.0 36.0 41.0 31.0 41.0 24-25 36.96807230099003 39.0 36.0 41.0 31.0 41.0 26-27 36.76287764531787 39.0 36.0 41.0 30.0 41.0 28-29 36.53258220783643 39.0 36.0 40.5 30.0 41.0 30-31 36.24173938380682 39.0 35.0 40.0 30.0 41.0 32-33 35.94462920806342 38.0 35.0 40.0 29.0 41.0 34-35 35.6277386242601 38.0 35.0 40.0 27.5 41.0 36-37 35.41157392048332 38.0 34.5 40.0 27.0 41.0 38-39 35.180361515863176 38.0 34.0 40.0 26.0 41.0 40-41 34.88530271647081 38.0 34.0 40.0 25.0 41.0 42-43 34.460901623421975 38.0 33.0 40.0 24.0 41.0 44-45 33.9451350268897 38.0 33.0 40.0 23.0 41.0 46-47 33.38158846754902 37.5 32.5 40.0 20.0 41.0 48-49 32.851580642908274 37.0 31.5 40.0 16.5 41.0 50-51 32.10362492579141 36.5 30.5 39.0 14.0 40.5 52-53 32.11965943714969 36.5 31.0 39.0 12.0 40.0 54-55 32.113234937839394 36.5 31.0 39.0 8.5 40.5 56-57 31.712160441148228 36.0 30.5 39.0 7.5 40.5 58-59 31.272238567512353 35.5 30.0 39.0 2.0 40.0 60-61 30.746443717151788 35.0 29.0 39.0 2.0 40.0 62-63 30.047669468404603 35.0 28.0 38.0 2.0 40.0 64-65 29.39557132973058 34.0 27.5 38.0 2.0 40.0 66-67 28.718257549632447 34.0 26.0 37.5 2.0 39.5 68-69 27.897614521310963 33.5 25.5 36.5 2.0 39.0 70-71 27.10167340364233 33.0 23.0 36.0 2.0 39.0 72-73 26.243957729479142 32.0 21.0 35.5 2.0 38.5 74-75 25.326829623631504 31.0 17.5 35.0 2.0 37.5 76-77 24.40670343629411 31.0 10.5 35.0 2.0 37.0 78-79 23.448534434967087 30.0 4.5 34.0 2.0 36.0 80-81 22.502203613085157 29.0 2.0 34.0 2.0 35.5 82-83 21.527525656527736 29.0 2.0 34.0 2.0 35.0 84-85 20.570146212742923 27.0 2.0 33.5 2.0 35.0 86-87 19.593391234219762 26.0 2.0 33.0 2.0 35.0 88-89 18.531846942606204 24.5 2.0 32.0 2.0 34.5 90-91 17.398581688376318 21.5 2.0 32.0 2.0 34.0 92-93 16.244630790452412 14.0 2.0 31.0 2.0 34.0 94-95 14.971944515985403 2.0 2.0 31.0 2.0 34.0 96-97 13.530384051265038 2.0 2.0 30.0 2.0 34.0 98-99 11.80399193090744 2.0 2.0 29.0 2.0 34.0 100-101 8.325636284070471 2.0 2.0 11.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6385562478467683 1101 2 0.49295164472293607 1101 3 0.501746877299972 1101 4 0.42897604526861954 1101 5 0.3943992621993502 1101 6 0.5637838987680865 1101 7 0.4749396462569919 1101 8 0.46016848141125877 1101 9 0.5531998332988763 1101 10-11 0.5859479889844152 1101 12-13 0.5846994644364756 1101 14-15 0.7281961191861441 1101 16-17 0.7350352758188095 1101 18-19 0.7320088892673269 1101 20-21 0.8643894059889092 1101 22-23 0.9321056612298761 1101 24-25 1.0495629411061813 1101 26-27 0.9600922723618481 1101 28-29 0.9471073210418766 1101 30-31 0.8374319389159837 1101 32-33 0.9111651047943639 1101 34-35 1.0603855659598125 1101 36-37 1.1206839066181047 1101 38-39 1.2590673712095821 1101 40-41 1.0729426193707567 1101 42-43 1.3007294111693355 1101 44-45 1.3069823749372063 1101 46-47 1.3016387306270403 1101 48-49 1.0757272129349786 1101 50-51 1.310396752466609 1101 52-53 1.2227246254380688 1101 54-55 1.447771154815321 1101 56-57 1.7487365283708023 1101 58-59 1.4870539646455079 1101 60-61 1.6201988876666285 1101 62-63 1.3493909152848609 1101 64-65 1.3324610384067874 1101 66-67 1.6924243806677879 1101 68-69 1.7975077661113197 1101 70-71 1.9715154026119244 1101 72-73 2.0661492058815476 1101 74-75 2.2255442606689044 1101 76-77 2.353654088547245 1101 78-79 2.3679405980308843 1101 80-81 2.705564586975626 1101 82-83 2.7514683234777344 1101 84-85 2.9509865266758197 1101 86-87 3.0041310789539395 1101 88-89 3.274785085734063 1101 90-91 3.168551383092229 1101 92-93 3.45279808114487 1101 94-95 3.2367078494087185 1101 96-97 3.190368819228455 1101 98-99 2.920612428310875 1101 100-101 1.9205779846816853 1102 1 0.6112457126977198 1102 2 0.5029634389749944 1102 3 0.33519272424593893 1102 4 0.32794205021397005 1102 5 0.3275162263055904 1102 6 0.4130587460119344 1102 7 0.505927106085089 1102 8 0.4725442724374531 1102 9 0.5827511552480544 1102 10-11 0.6519845996021445 1102 12-13 0.793160447742892 1102 14-15 0.7775303615083757 1102 16-17 0.7564020649487233 1102 18-19 0.7841445691805262 1102 20-21 0.6896104573968387 1102 22-23 0.6186567627936412 1102 24-25 0.7765653605020262 1102 26-27 0.8280768720232814 1102 28-29 0.6217187101238366 1102 30-31 0.45523126952557647 1102 32-33 0.5837668393001749 1102 34-35 0.5897992983925562 1102 36-37 0.7611307138147794 1102 38-39 0.9191688938063507 1102 40-41 0.8902456892668411 1102 42-43 1.1213753462694669 1102 44-45 1.1570768560990956 1102 46-47 1.0622506815706956 1102 48-49 0.9178368098718721 1102 50-51 1.321283125625115 1102 52-53 1.2925285248067055 1102 54-55 1.004301676983431 1102 56-57 1.4046717677553637 1102 58-59 1.429195680939248 1102 60-61 1.3785207072751184 1102 62-63 1.6024233337362048 1102 64-65 1.64244265075612 1102 66-67 1.8028205173842373 1102 68-69 1.8936955526426225 1102 70-71 1.7960680323426388 1102 72-73 1.944474664271091 1102 74-75 2.121050396343975 1102 76-77 2.2664557864836183 1102 78-79 2.554147498758155 1102 80-81 2.818846924901532 1102 82-83 2.9102398140822956 1102 84-85 2.993625956773684 1102 86-87 3.134042222463588 1102 88-89 3.111893629180262 1102 90-91 2.8955388174912464 1102 92-93 2.9234132591605757 1102 94-95 2.7751779690222893 1102 96-97 2.795306330277997 1102 98-99 3.012691147072454 1102 100-101 2.205027432870528 1103 1 0.5100101694964501 1103 2 0.43582228520196864 1103 3 0.41468017205260566 1103 4 0.36565861663307686 1103 5 0.27691009825892365 1103 6 0.2102032749417475 1103 7 0.22792325339521113 1103 8 0.37206600240427434 1103 9 0.27490472635552976 1103 10-11 0.39451083812993204 1103 12-13 0.3194333166408967 1103 14-15 0.46773326885958966 1103 16-17 0.5309143253162745 1103 18-19 0.6289535338327568 1103 20-21 0.6148411545214714 1103 22-23 0.6161961408509953 1103 24-25 0.6094293652655765 1103 26-27 0.6145758027323396 1103 28-29 0.5218683076842936 1103 30-31 0.6612160562643083 1103 32-33 0.6420257300379575 1103 34-35 0.7815071719406106 1103 36-37 0.860659665732797 1103 38-39 0.8374424937426781 1103 40-41 0.8964528826249989 1103 42-43 0.8588157099020748 1103 44-45 0.9506412132643831 1103 46-47 1.057778819913807 1103 48-49 0.9903957535214829 1103 50-51 1.0631784014373267 1103 52-53 1.2601136074980488 1103 54-55 1.4419841970611955 1103 56-57 1.6347354240966077 1103 58-59 1.764182349806898 1103 60-61 1.9139401036673824 1103 62-63 2.2005137908857115 1103 64-65 2.1678446112947896 1103 66-67 2.162436545399512 1103 68-69 2.1148113588408286 1103 70-71 2.129817536754665 1103 72-73 2.3396123750894233 1103 74-75 2.4525071523319255 1103 76-77 2.607260547665483 1103 78-79 2.7926603958365064 1103 80-81 2.9499735345491764 1103 82-83 3.237609830014801 1103 84-85 3.3265888580525598 1103 86-87 3.3322115315292002 1103 88-89 3.5211338946532926 1103 90-91 3.6568654162322645 1103 92-93 3.6086339045082383 1103 94-95 3.4937910006647552 1103 96-97 3.6908487651367334 1103 98-99 3.379963327674293 1103 100-101 2.2669164594841558 1104 1 0.561351987632257 1104 2 0.49264440660877895 1104 3 0.5254241363663859 1104 4 0.5306966942109597 1104 5 0.4461241270030314 1104 6 0.4957217049955531 1104 7 0.4683539510787824 1104 8 0.5654913817390579 1104 9 0.5755037610956109 1104 10-11 0.5813114077823087 1104 12-13 0.633176672219534 1104 14-15 0.7717632922951907 1104 16-17 0.7641076823710691 1104 18-19 0.7314787302658274 1104 20-21 0.7580295770258303 1104 22-23 0.6974643906039191 1104 24-25 0.9701159004098372 1104 26-27 0.932670981915571 1104 28-29 0.9678639410992105 1104 30-31 0.8905180071690424 1104 32-33 0.9628330843457036 1104 34-35 1.0587819504833291 1104 36-37 1.1239210239853676 1104 38-39 1.1664165993026927 1104 40-41 1.1543724143066427 1104 42-43 1.0030893117443043 1104 44-45 1.194465222734216 1104 46-47 1.2932198576596008 1104 48-49 1.4362515043815165 1104 50-51 1.4539049612684067 1104 52-53 1.470682964642812 1104 54-55 1.5265931796300052 1104 56-57 1.7966834327735448 1104 58-59 1.893674512869186 1104 60-61 1.9436130596599064 1104 62-63 1.9136634539726067 1104 64-65 1.9656451514242121 1104 66-67 2.186876769155365 1104 68-69 2.2971351243948526 1104 70-71 2.2236343060721637 1104 72-73 2.5021455005436906 1104 74-75 2.537943339878588 1104 76-77 2.6079194718210204 1104 78-79 2.9927687872433637 1104 80-81 3.1117893928727476 1104 82-83 3.154682109228819 1104 84-85 3.161712313193842 1104 86-87 3.260128574847805 1104 88-89 3.4516318062559534 1104 90-91 3.221339886611606 1104 92-93 3.6050371655290867 1104 94-95 3.4593822821154863 1104 96-97 3.330764076442195 1104 98-99 3.102346082896693 1104 100-101 1.8902743156117836 1105 1 0.4438942861457882 1105 2 0.3869056139758378 1105 3 0.41237125921132645 1105 4 0.10271064924236839 1105 5 0.1649647571197832 1105 6 0.24382750211250936 1105 7 0.16451979549730567 1105 8 0.2344312702272262 1105 9 0.315539130251139 1105 10-11 0.3286972472780434 1105 12-13 0.48145730854328406 1105 14-15 0.40955906083857485 1105 16-17 0.5457888944468792 1105 18-19 0.5297489324211497 1105 20-21 0.49944065115658276 1105 22-23 0.5556514672174657 1105 24-25 0.5860605137035009 1105 26-27 0.41645521401428454 1105 28-29 0.4670918126358643 1105 30-31 0.5602866094185472 1105 32-33 0.6145848206983473 1105 34-35 0.5824119976778732 1105 36-37 0.6924653504731779 1105 38-39 0.7979878512958862 1105 40-41 0.8361393738434728 1105 42-43 0.9755956792624687 1105 44-45 0.9051708133081462 1105 46-47 1.0960523330724143 1105 48-49 1.0222951352580978 1105 50-51 0.9399942081889847 1105 52-53 1.0787032283329765 1105 54-55 1.16526755866321 1105 56-57 1.2905822534449705 1105 58-59 1.156583318237164 1105 60-61 1.399839047695398 1105 62-63 1.5209554662153657 1105 64-65 1.5771623330215476 1105 66-67 1.6474792219875773 1105 68-69 1.7948792036731298 1105 70-71 1.8204129572284842 1105 72-73 1.9931147277731256 1105 74-75 2.198723708659557 1105 76-77 2.1638795223330973 1105 78-79 2.3996226981837196 1105 80-81 2.7180173874790867 1105 82-83 3.0512930110620182 1105 84-85 2.753503833157886 1105 86-87 2.9443360596246215 1105 88-89 3.1141326088842938 1105 90-91 2.9750446852589114 1105 92-93 3.0325697254854234 1105 94-95 3.021352279868127 1105 96-97 2.993330025737487 1105 98-99 2.896672169387923 1105 100-101 2.1307056480818467 1106 1 0.21418497340675557 1106 2 0.08073101197189558 1106 3 0.01725151797461777 1106 4 0.01140914154584749 1106 5 0.14041727679570215 1106 6 -0.13961575645059554 1106 7 0.21118279213633429 1106 8 -0.0488834495687982 1106 9 0.16139401841187606 1106 10-11 0.2660532996628575 1106 12-13 0.30609921744932933 1106 14-15 0.41610136800408526 1106 16-17 -0.09196370530236919 1106 18-19 0.2783883443036075 1106 20-21 0.2882111567775283 1106 22-23 0.24284579254698002 1106 24-25 0.3431450279428603 1106 26-27 0.39832018509306266 1106 28-29 0.5714047097100305 1106 30-31 0.4577236043507398 1106 32-33 0.47835993580375913 1106 34-35 0.3391556734651715 1106 36-37 0.4417477195901611 1106 38-39 0.5322019473628075 1106 40-41 0.7709444058201242 1106 42-43 0.37504530681613346 1106 44-45 0.9516124229928025 1106 46-47 1.0752456469493339 1106 48-49 0.7636432645975972 1106 50-51 0.6148331396314717 1106 52-53 0.8172468156181765 1106 54-55 0.71473233166585 1106 56-57 1.1593698536683341 1106 58-59 1.0662915510398356 1106 60-61 1.3489674600309023 1106 62-63 1.4486306793052464 1106 64-65 1.4148294597586677 1106 66-67 1.5509821667896553 1106 68-69 1.5476085452807062 1106 70-71 1.5661994933194308 1106 72-73 1.570632954991737 1106 74-75 1.8639033995343546 1106 76-77 2.2492384826441167 1106 78-79 2.1953287658456873 1106 80-81 2.153048547594139 1106 82-83 2.3332866526074376 1106 84-85 2.5637719207749683 1106 86-87 2.3992115772038787 1106 88-89 2.285498096225332 1106 90-91 2.180977355177678 1106 92-93 2.1994733635539703 1106 94-95 2.169289996513898 1106 96-97 2.247956367755968 1106 98-99 2.195768842130329 1106 100-101 1.5516292834110423 1107 1 0.24320286203516162 1107 2 0.14316796988690328 1107 3 0.1711433224330392 1107 4 0.1463423493143523 1107 5 0.0466483545528007 1107 6 0.1204483441900237 1107 7 0.1434508761262805 1107 8 0.1617379604144773 1107 9 0.2977783001366774 1107 10-11 0.39067677828568037 1107 12-13 0.3352432864217647 1107 14-15 0.30438098364644617 1107 16-17 0.3181018956533208 1107 18-19 0.3279611904987476 1107 20-21 0.3332066314682365 1107 22-23 0.39994493721351176 1107 24-25 0.4050403336493602 1107 26-27 0.2585200578355611 1107 28-29 0.5334244261581773 1107 30-31 0.5263813558118855 1107 32-33 0.5730500771436979 1107 34-35 0.6539780682193523 1107 36-37 0.7196456935493032 1107 38-39 0.6762324501786594 1107 40-41 0.6124084209556102 1107 42-43 0.7115964953862459 1107 44-45 0.7915670185860861 1107 46-47 0.792526019417302 1107 48-49 0.6132465530830444 1107 50-51 0.940477165902621 1107 52-53 1.1810353907061213 1107 54-55 1.1766694666693311 1107 56-57 1.5190298694511348 1107 58-59 1.267545609263852 1107 60-61 1.5764525395899192 1107 62-63 1.4536955890901488 1107 64-65 1.7262747350613523 1107 66-67 2.051537283364137 1107 68-69 2.0977594615885167 1107 70-71 2.1127909441907775 1107 72-73 2.070749261467377 1107 74-75 2.4185986034758997 1107 76-77 2.553455188665204 1107 78-79 2.9589224538535 1107 80-81 2.941602477252701 1107 82-83 2.7146496875627264 1107 84-85 2.8017136834169136 1107 86-87 2.523711953052036 1107 88-89 2.837127656381682 1107 90-91 2.9339692014141043 1107 92-93 2.4770826871529508 1107 94-95 2.472756545001902 1107 96-97 2.449123997573613 1107 98-99 2.332727731702267 1107 100-101 1.4300114359707727 1108 1 0.20516471727972174 1108 2 0.1927508835493974 1108 3 0.12921947923970833 1108 4 0.1440180376304383 1108 5 0.1071182318163153 1108 6 0.2819868879278644 1108 7 0.18746468631005797 1108 8 0.1506164036102433 1108 9 0.13548354123214068 1108 10-11 0.12839735243265693 1108 12-13 0.03373510560507498 1108 14-15 0.14669446527548757 1108 16-17 0.34751029085936835 1108 18-19 0.16118493103270026 1108 20-21 0.058087576660156515 1108 22-23 0.22426357939600194 1108 24-25 0.2814095913035217 1108 26-27 0.2823550006422906 1108 28-29 0.18057661626217225 1108 30-31 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0.2756590909434067 2316 74-75 0.7202555915805604 2316 76-77 0.7487046110041895 2316 78-79 0.46395592470659963 2316 80-81 0.47787603857352323 2316 82-83 0.5486106414168148 2316 84-85 0.799460597022069 2316 86-87 1.0176772748329554 2316 88-89 0.8398841033098918 2316 90-91 0.6668539346760589 2316 92-93 0.6443734778472248 2316 94-95 0.6165655600908817 2316 96-97 0.6869075867023167 2316 98-99 0.7618372744120663 2316 100-101 0.793312136264559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5803.0 3 2009.0 4 2073.0 5 2394.0 6 2821.0 7 3680.0 8 4738.0 9 5989.0 10 7791.0 11 10104.0 12 12745.0 13 16027.0 14 18403.0 15 22193.0 16 27045.0 17 32348.0 18 30817.0 19 28202.0 20 30504.0 21 34073.0 22 38099.0 23 41738.0 24 47392.0 25 53094.0 26 59070.0 27 66035.0 28 73343.0 29 82293.0 30 91658.0 31 102959.0 32 117343.0 33 133687.0 34 151170.0 35 165571.0 36 163313.0 37 116441.0 38 29309.0 39 398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40736194437187 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25.715874582478687 28.747077248502844 22.744375314991366 44-45 22.494364860193144 26.108330566127936 28.515103484386824 22.8822010892921 46-47 22.73518510079352 26.303870221098187 28.073436153918152 22.887508524190146 48-49 22.98253828958628 26.278820881577335 27.93458898726882 22.80405184156756 50-51 22.771298184819607 26.56870195160883 27.589404967359492 23.070594896212064 52-53 23.10066313489491 26.59982930497439 27.519607008536934 22.77990055159377 54-55 22.549206053837782 26.322063536975104 27.818961121098855 23.309769288088255 56-57 22.784485814078938 26.77119034151913 27.66207540667726 22.782248437724675 58-59 23.041013598443087 26.126930625049283 27.723105894113797 23.108949882393834 60-61 22.87043368125113 26.187558014341995 28.11869550198879 22.823312802418087 62-63 22.77681989697453 26.321400281970764 27.95041309234938 22.951366728705327 64-65 22.603295885954207 26.216857923512176 27.963407906131803 23.216438284401814 66-67 22.674053607340593 26.453812840584444 27.693415406005716 23.17871814606924 68-69 22.481255729689614 26.249590736456106 27.909127122713578 23.3600264111407 70-71 22.4436225119754 26.49104113315715 27.75102631654344 23.314310038324003 72-73 22.73876997730912 26.38822038643262 27.479352824136377 23.39365681212189 74-75 22.47877823770011 26.643155146021552 27.387137879379075 23.490928736899264 76-77 22.585741137219927 26.476570687377887 27.45094240833411 23.48674576706808 78-79 22.797071447651433 26.407830355040453 27.188344726180137 23.60675347112798 80-81 22.545324268661506 26.90375025512125 27.197862766049173 23.353062710168068 82-83 22.860349390518927 26.694978960876593 26.94901467704311 23.49565697156137 84-85 22.785896684864746 26.274618087283656 26.90841761847555 24.031067609376052 86-87 22.94766957106313 25.9889371742376 27.23059940282349 23.83279385187578 88-89 22.846406381790263 26.077687064917317 27.104598850048724 23.971307703243696 90-91 22.743124681556086 26.224550753581237 27.106082657465823 23.92624190739685 92-93 23.029038623574046 25.822693696328862 27.06496090492759 24.0833067751695 94-95 22.796651680640736 25.79941465810712 26.97787571376918 24.426057947482963 96-97 23.048965315120856 25.749346312306354 26.665436880593045 24.536251491979737 98-99 22.936929331283977 25.720642562435042 26.367401577510257 24.975026528770723 100-101 23.8189405499673 24.773797250436672 26.608496733097418 24.798765466498608 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1290.0 1 917.5 2 748.5 3 942.0 4 772.5 5 710.0 6 1160.5 7 2553.5 8 4064.5 9 4364.0 10 3528.5 11 2661.5 12 2469.0 13 2537.0 14 2752.5 15 2960.5 16 3089.5 17 3243.0 18 3414.5 19 3690.5 20 3997.0 21 4406.5 22 5052.5 23 5761.0 24 6521.5 25 7691.0 26 9347.0 27 11100.5 28 12529.0 29 14140.5 30 16252.0 31 18739.0 32 21855.5 33 24971.5 34 28022.0 35 30432.5 36 33059.0 37 36350.0 38 38840.5 39 40668.5 40 43568.0 41 48753.0 42 53878.0 43 57842.5 44 61377.0 45 64827.5 46 66388.5 47 67811.5 48 72731.0 49 75293.5 50 73103.0 51 70097.5 52 67507.5 53 66594.5 54 65784.0 55 62107.0 56 57388.5 57 52947.5 58 48093.0 59 42919.0 60 37123.5 61 31407.0 62 26110.0 63 21209.5 64 17312.5 65 14126.5 66 11258.0 67 9114.5 68 6916.0 69 4763.5 70 3689.5 71 3026.0 72 2517.5 73 2136.0 74 1723.0 75 1377.5 76 1007.0 77 655.5 78 433.0 79 285.5 80 171.5 81 112.0 82 71.5 83 44.0 84 32.0 85 18.0 86 11.5 87 13.0 88 10.5 89 8.0 90 6.0 91 3.0 92 1.5 93 1.5 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3826653105411116 2 0.030665607375460528 3 0.03748624958530496 4 0.045998411063190796 5 0.06940685512737685 6 0.058875783555377065 7 0.04037820188227899 8 0.04299732849085925 9 0.03928689912870388 10-11 0.03912320371566762 12-13 0.030992998201533062 14-15 0.028483001868310317 16-17 0.022699097274362243 18-19 0.0293560440711704 20-21 0.038059183530931885 22-23 0.03691331563967802 24-25 0.04648949730229959 26-27 0.04106026610326343 28-29 0.03533092664699412 30-31 0.03835929178816504 32-33 0.03219343123046568 34-35 0.03355755967243457 36-37 0.03653135967592674 38-39 0.016942475249253547 40-41 0.014050522952279513 42-43 0.015305521118890887 44-45 0.01077661469155419 46-47 0.02108942571283896 48-49 0.017460844057201726 50-51 0.011431396343699255 52-53 0.008948682579315885 54-55 0.008102922945295176 56-57 0.009194225698870283 58-59 0.003955972481709765 60-61 0.008239335789492064 62-63 0.011595091756735522 64-65 0.00791194496341953 66-67 0.0037377119309947443 68-69 0.006111295420020604 70-71 0.014705304604424577 72-73 0.011895200013968675 74-75 0.012931937629865027 76-77 0.005811187162787449 78-79 0.008866834872797751 80-81 0.011458678912538633 82-83 0.013777697263885736 84-85 0.008321183496010198 86-87 0.002619126608580259 88-89 0.018197473415864925 90-91 0.02840115416179218 92-93 0.011786069738611163 94-95 0.02234442387945033 96-97 0.02046192662953327 98-99 0.012413568821916852 100-101 0.1279552478566814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832672.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.25659027611474 #Duplication Level Percentage of deduplicated Percentage of total 1 76.44694713952084 37.655159531111806 2 12.706891651982023 12.517963115693226 3 4.310111788525796 6.369042312350018 4 2.02896653378947 3.9975989295526655 5 1.0959221576228124 2.6990694346271256 6 0.68841060998965 2.034525561479425 7 0.45561768656222784 1.5709521596682845 8 0.33297328686107885 1.3120903011045906 9 0.23495536197403163 1.0415789998137996 >10 1.454798219945787 13.596329097869276 >50 0.14332024907933671 4.877321969305103 >100 0.09527750389454506 8.629610644536314 >500 0.004132480371914734 1.3533450150232063 >1k 0.0013402639044047789 0.9853011877668953 >5k 2.2337731740079645E-4 0.7693711396381927 >10k+ 1.1168865870039823E-4 0.5907406004601297 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10738 0.5859204483944754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7968 0.4347750170243229 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6017 0.3283184334130712 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1916 0.10454680379249533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1910 0.10421941296642279 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1897 0.10351006617659898 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1873 0.10220050287230885 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.456513767875539E-5 0.0 0.0 0.0 0.0 6 5.456513767875539E-5 0.0 0.0 0.0 0.0 7 5.456513767875539E-5 0.0 0.0 0.0 0.0 8 5.456513767875539E-5 0.0 0.0 0.0 0.0 9 5.456513767875539E-5 0.0 0.0 0.0 0.0 10-11 5.456513767875539E-5 0.0 0.0 0.0 0.0 12-13 5.456513767875539E-5 0.0 0.0 0.0 0.0 14-15 5.456513767875539E-5 0.0 0.0 0.0 0.0 16-17 5.456513767875539E-5 0.0 0.0 0.0 0.0 18-19 5.456513767875539E-5 0.0 0.0 0.0 0.0 20-21 5.456513767875539E-5 0.0 0.0 2.7282568839377695E-5 0.0 22-23 5.456513767875539E-5 5.456513767875539E-5 0.0 1.0913027535751078E-4 0.0 24-25 5.456513767875539E-5 5.456513767875539E-5 0.0 2.7282568839377696E-4 2.7282568839377695E-5 26-27 5.456513767875539E-5 5.456513767875539E-5 0.0 0.0010367376158963524 5.456513767875539E-5 28-29 5.456513767875539E-5 5.456513767875539E-5 0.0 0.0037104293621553664 5.456513767875539E-5 30-31 5.456513767875539E-5 5.456513767875539E-5 0.0 0.013232045887098181 5.456513767875539E-5 32-33 5.456513767875539E-5 5.456513767875539E-5 0.0 0.029983543154476086 5.456513767875539E-5 34-35 5.456513767875539E-5 5.456513767875539E-5 0.0 0.054892528504827925 5.456513767875539E-5 36-37 5.456513767875539E-5 5.456513767875539E-5 0.0 0.09900844231810166 5.456513767875539E-5 38-39 5.456513767875539E-5 5.456513767875539E-5 0.0 0.1612399818407222 5.456513767875539E-5 40-41 5.456513767875539E-5 5.456513767875539E-5 0.0 0.23026488100434775 5.456513767875539E-5 42-43 5.456513767875539E-5 5.456513767875539E-5 0.0 0.30229086274030487 5.456513767875539E-5 44-45 5.456513767875539E-5 5.456513767875539E-5 0.0 0.39030442981613733 5.456513767875539E-5 46-47 5.456513767875539E-5 5.456513767875539E-5 0.0 0.49621536205060157 5.456513767875539E-5 48-49 5.456513767875539E-5 5.456513767875539E-5 0.0 0.6166679034764541 5.456513767875539E-5 50-51 5.456513767875539E-5 5.456513767875539E-5 0.0 0.7486882540902027 5.456513767875539E-5 52-53 5.456513767875539E-5 5.456513767875539E-5 0.0 0.8792899111243038 5.456513767875539E-5 54-55 5.456513767875539E-5 5.456513767875539E-5 0.0 1.018731120462363 5.456513767875539E-5 56-57 5.456513767875539E-5 5.456513767875539E-5 0.0 1.1862733757049817 5.456513767875539E-5 58-59 5.456513767875539E-5 8.184770651813308E-5 0.0 1.368984739222294 5.456513767875539E-5 60-61 5.456513767875539E-5 1.6369541303626618E-4 0.0 1.56907509909029 5.456513767875539E-5 62-63 5.456513767875539E-5 1.6369541303626618E-4 0.0 1.7702840442807006 5.456513767875539E-5 64-65 5.456513767875539E-5 2.1826055071502156E-4 0.0 1.9769222206701471 5.456513767875539E-5 66-67 5.456513767875539E-5 2.1826055071502156E-4 0.0 2.209069598924412 5.456513767875539E-5 68-69 5.456513767875539E-5 2.1826055071502156E-4 0.0 2.4767934469452255 5.456513767875539E-5 70-71 5.456513767875539E-5 2.1826055071502156E-4 0.0 2.7679803041679034 5.456513767875539E-5 72-73 5.456513767875539E-5 2.1826055071502156E-4 0.0 3.0560842311117318 5.456513767875539E-5 74-75 5.456513767875539E-5 2.1826055071502156E-4 0.0 3.346043372736638 5.456513767875539E-5 76-77 5.456513767875539E-5 2.4554311955439923E-4 0.0 3.665167580450839 5.456513767875539E-5 78-79 5.456513767875539E-5 2.7282568839377696E-4 0.0 4.013511419392014 5.456513767875539E-5 80-81 5.456513767875539E-5 2.7282568839377696E-4 0.0 4.392930104241239 5.456513767875539E-5 82-83 5.456513767875539E-5 2.7282568839377696E-4 0.0 4.77646845698521 5.456513767875539E-5 84-85 5.456513767875539E-5 2.7282568839377696E-4 0.0 5.155477903301845 5.456513767875539E-5 86-87 1.0913027535751078E-4 2.7282568839377696E-4 0.0 5.55246656248363 5.456513767875539E-5 88-89 1.0913027535751078E-4 2.7282568839377696E-4 0.0 5.973845838207819 5.456513767875539E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5190 0.0 47.969635 1 GTATCAA 9125 0.0 39.56638 1 TATCAAC 11940 0.0 30.095083 2 ATCAACG 12040 0.0 29.8846 3 TCAACGC 12255 0.0 29.243956 4 CAACGCA 12510 0.0 28.419888 5 AACGCAG 12965 0.0 27.41352 6 GTACATG 11165 0.0 27.16825 1 TACATGG 11355 0.0 26.496874 2 ACATGGG 11690 0.0 24.721064 3 ACGCAGA 14605 0.0 24.103563 7 CGCAGAG 14800 0.0 23.657583 8 GATGTGC 1075 0.0 23.422434 8 GCAGAGT 15320 0.0 22.294573 9 GAGTACT 7750 0.0 22.217129 12-13 AGTACTT 7925 0.0 22.056175 12-13 CATGGGG 4990 0.0 21.050858 4 CATGGGA 6390 0.0 21.05056 4 AGAGTAC 14090 0.0 20.597935 10-11 GTACTTT 8460 0.0 20.40955 14-15 >>END_MODULE