FastQCFastQC Report
Thu 26 May 2016
SRR938328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832672
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102340.5584196190043826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85570.46691388311710985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.35058100958600336No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.11567809187896143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19770.10787527719089941No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG19210.1048196294808891No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC18390.10034528819123116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.046.100081
GTATCAA93850.039.0788151
TATCAAC124750.029.1659052
ATCAACG127100.028.5129763
TCAACGC128250.028.2573034
GTACATG109550.027.8407541
CAACGCA130850.027.7321285
AACGCAG134200.027.216826
TACATGG113800.026.5044442
ACATGGG116100.025.0779383
ACGCAGA151900.023.9203327
CGCAGAG152800.023.7483588
CATGGGG48500.022.915944
AGTACTT84300.022.64967512-13
GAGTACT82900.022.11547912-13
GCAGAGT162950.021.9776139
GTACACG2701.0004442E-921.114261
GTACTTT87350.020.98881514-15
ATGGGAG26750.020.951825
AGAGTAC148900.020.6701610-11
Produced by FastQC (version 0.11.5)