FastQCFastQC Report
Thu 26 May 2016
SRR938327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775113
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131540.47399871644866354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101730.3665796672063444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72320.2606020007113224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.053.2884861
GTATCAA119300.042.1785741
ATCAACG155900.032.0617753
TATCAAC157250.031.6957912
TCAACGC158850.031.5561754
CAACGCA160800.031.0847685
AACGCAG167900.029.8625646
GTACATG140900.026.4300161
ACGCAGA190300.026.1717137
CGCAGAG192000.025.8652638
TACATGG145250.024.8844722
ACATGGG147350.023.8843023
GCAGAGT202200.023.6665259
GAGTACT107900.022.23908812-13
AGTACTT111100.021.57715212-13
AGAGTAC183100.021.2815810-11
GTACTTT115800.020.73943714-15
CATGGGG67100.020.6962344
GGACCGA7850.020.5842296
CAGAGTA198100.020.14991210-11
Produced by FastQC (version 0.11.5)