##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938327_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2775113 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.315718314893843 31.0 31.0 34.0 28.0 34.0 2 31.356050005891653 31.0 31.0 34.0 28.0 34.0 3 31.401543648853217 31.0 31.0 34.0 28.0 34.0 4 35.06046888901461 37.0 35.0 37.0 32.0 37.0 5 35.04674908733446 37.0 35.0 37.0 32.0 37.0 6 35.072826223652875 37.0 35.0 37.0 32.0 37.0 7 34.99109802015269 37.0 35.0 37.0 32.0 37.0 8 34.96682153123134 37.0 35.0 37.0 32.0 37.0 9 36.480277019350204 39.0 37.0 39.0 32.0 39.0 10-11 36.31992643182458 39.0 35.0 39.0 32.0 39.0 12-13 36.18754353426329 39.0 35.0 39.0 32.0 39.0 14-15 37.179812497725315 39.0 36.5 41.0 31.5 41.0 16-17 37.03329720267247 39.0 36.0 41.0 31.0 41.0 18-19 37.19380832420157 39.5 36.5 41.0 31.0 41.0 20-21 37.18315740656327 40.0 37.0 41.0 31.0 41.0 22-23 37.100261322692084 39.0 36.0 41.0 31.0 41.0 24-25 36.97111144663299 39.0 36.0 41.0 30.5 41.0 26-27 36.78269281286924 39.0 36.0 41.0 30.0 41.0 28-29 36.583364533264046 39.0 36.0 41.0 30.0 41.0 30-31 36.322605061487586 39.0 35.5 40.0 30.0 41.0 32-33 36.06346354184497 38.5 35.0 40.0 29.0 41.0 34-35 35.81964013717639 38.0 35.0 40.0 28.5 41.0 36-37 35.55520315749305 38.0 35.0 40.0 27.0 41.0 38-39 35.44359112583884 38.0 35.0 40.0 27.0 41.0 40-41 35.33967968151207 38.0 34.5 40.0 26.5 41.0 42-43 35.04515924216419 38.0 34.0 40.0 25.5 41.0 44-45 34.6783702141138 38.0 33.5 40.0 24.0 41.0 46-47 34.26200374543307 38.0 33.0 40.0 23.5 41.0 48-49 33.888589401584724 38.0 33.0 40.0 21.5 41.0 50-51 33.181676746136105 37.5 32.0 39.5 20.0 40.5 52-53 33.206207999458044 37.0 32.5 39.5 20.0 40.5 54-55 33.323066844485254 37.5 33.0 40.0 19.0 41.0 56-57 33.03166429619262 37.0 32.0 40.0 17.5 41.0 58-59 32.690644669244094 37.0 31.5 40.0 14.0 41.0 60-61 32.24190510440476 36.0 31.0 39.0 11.0 41.0 62-63 31.70580999764694 35.5 30.5 39.0 8.0 40.0 64-65 31.21663442173346 35.0 30.0 39.0 6.5 40.0 66-67 30.716100569598424 35.0 29.0 38.0 2.0 40.0 68-69 30.174688742404363 34.5 29.0 38.0 2.0 40.0 70-71 29.572044273512468 34.0 28.0 37.0 2.0 39.5 72-73 28.955645049408798 34.0 27.0 36.5 2.0 39.0 74-75 28.305507379339147 34.0 26.0 36.0 2.0 39.0 76-77 27.594624795458778 33.0 25.5 35.5 2.0 38.0 78-79 26.856063338682063 32.5 24.5 35.0 2.0 37.0 80-81 26.19789446411732 32.0 22.5 35.0 2.0 36.5 82-83 25.497103541369306 31.5 20.0 35.0 2.0 36.0 84-85 24.820789099398837 31.0 18.0 34.0 2.0 35.5 86-87 24.091476455192996 31.0 10.5 34.0 2.0 35.0 88-89 23.322202375182563 30.0 4.5 34.0 2.0 35.0 90-91 22.456975445684556 29.5 2.0 34.0 2.0 35.0 92-93 21.551413401904718 29.0 2.0 34.0 2.0 35.0 94-95 20.572525335004375 28.5 2.0 34.0 2.0 35.0 96-97 19.36238632444877 26.5 2.0 33.5 2.0 35.0 98-99 17.866180584358187 24.0 2.0 33.0 2.0 34.5 100-101 14.212276761342693 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45629665389860463 1101 2 0.3905861163702369 1101 3 0.32979132334158123 1101 4 0.28249819583143676 1101 5 0.2849542614954643 1101 6 0.4450716137444033 1101 7 0.37583336206120777 1101 8 0.4573900290225694 1101 9 0.6275571428239672 1101 10-11 0.6225790117672574 1101 12-13 0.6589764240813238 1101 14-15 0.7977315582700868 1101 16-17 0.6080611880922504 1101 18-19 0.7551616980362894 1101 20-21 0.8174395022647829 1101 22-23 0.8229834751534213 1101 24-25 0.7467677947283562 1101 26-27 0.6720031706022169 1101 28-29 0.6586125659562967 1101 30-31 0.595329387367876 1101 32-33 0.6491657023506434 1101 34-35 0.6461646356169339 1101 36-37 0.6833185356297378 1101 38-39 0.8251920677950224 1101 40-41 0.7866986718515392 1101 42-43 0.8879631754844297 1101 44-45 0.9591704155401928 1101 46-47 0.9434326749149662 1101 48-49 0.6306303735388639 1101 50-51 0.749422282121408 1101 52-53 0.7903639208050137 1101 54-55 0.8412925900040946 1101 56-57 0.7059219659766995 1101 58-59 0.7441096678390196 1101 60-61 0.9274891192033223 1101 62-63 0.8543640757655595 1101 64-65 0.9936827304799003 1101 66-67 0.9301119495403043 1101 68-69 1.0894932426167507 1101 70-71 1.0458453535613756 1101 72-73 1.2186744689430462 1101 74-75 1.076685176682723 1101 76-77 1.1676763044085448 1101 78-79 1.0864850957049548 1101 80-81 1.222455290107252 1101 82-83 1.1535000066231547 1101 84-85 1.103167010433605 1101 86-87 1.0927638853593038 1101 88-89 1.0324915863734212 1101 90-91 0.6791070141023638 1101 92-93 0.7230147483201357 1101 94-95 0.8795925789073173 1101 96-97 0.6027373442348143 1101 98-99 0.6160897307053297 1101 100-101 0.7521591892113602 1102 1 0.40751397055462135 1102 2 0.33519178656952775 1102 3 0.33361214685902496 1102 4 0.3440502809715156 1102 5 0.28338049906596297 1102 6 0.3470707984675556 1102 7 0.3814337432067987 1102 8 0.430113090306655 1102 9 0.5236535474823185 1102 10-11 0.6457005268063227 1102 12-13 0.6731772441883592 1102 14-15 0.7137255483477176 1102 16-17 0.7271745269437062 1102 18-19 0.6516911361905144 1102 20-21 0.6124262814601522 1102 22-23 0.7483794955192451 1102 24-25 0.659299419639531 1102 26-27 0.601937516664556 1102 28-29 0.6428825528635613 1102 30-31 0.7522521051232758 1102 32-33 0.7621404602375677 1102 34-35 0.7600187800995073 1102 36-37 0.8574864080133011 1102 38-39 0.7104267745844268 1102 40-41 0.731210958454291 1102 42-43 0.7841251535773424 1102 44-45 0.7411253528319364 1102 46-47 0.7186592362543038 1102 48-49 0.7750537408079197 1102 50-51 0.944589241892551 1102 52-53 0.8512032607674129 1102 54-55 0.6851847099946937 1102 56-57 0.7934191758124243 1102 58-59 0.7968977463544249 1102 60-61 0.8211868973909304 1102 62-63 0.8921408143214933 1102 64-65 0.9642015436885565 1102 66-67 0.6601818335499701 1102 68-69 0.7187597056837589 1102 70-71 0.98147748952087 1102 72-73 0.9259842968347307 1102 74-75 1.0848337005120037 1102 76-77 1.0131454801372968 1102 78-79 0.950687928389506 1102 80-81 1.0804033246240543 1102 82-83 1.13159555209387 1102 84-85 1.153668508157196 1102 86-87 1.3778611514008254 1102 88-89 1.4126567933725092 1102 90-91 1.1975291972439948 1102 92-93 1.2512209855539922 1102 94-95 1.3003366910203304 1102 96-97 1.3914617872448396 1102 98-99 1.3494447266911536 1102 100-101 1.4542764939382824 1103 1 0.29593906375052725 1103 2 0.30758408157555195 1103 3 0.23335640897378696 1103 4 0.2103231286872571 1103 5 0.20406283340923181 1103 6 0.37423387067842384 1103 7 0.2659301891764372 1103 8 0.32985904464894844 1103 9 0.4595718523779979 1103 10-11 0.4981810855798585 1103 12-13 0.5263104387120379 1103 14-15 0.48377072377177655 1103 16-17 0.5853347653785619 1103 18-19 0.5389953401772587 1103 20-21 0.34928560588061686 1103 22-23 0.5176141644666572 1103 24-25 0.48541443893444836 1103 26-27 0.6204292624722356 1103 28-29 0.6378789886093301 1103 30-31 0.6306552628198432 1103 32-33 0.5879474941223108 1103 34-35 0.47574274676544803 1103 36-37 0.5342230741417424 1103 38-39 0.506483884443135 1103 40-41 0.6189352579249672 1103 42-43 0.5408138591572538 1103 44-45 0.5551838150624775 1103 46-47 0.3752195440704966 1103 48-49 0.3311632815162113 1103 50-51 0.4707782256323654 1103 52-53 0.4733791974329762 1103 54-55 0.5716368069113926 1103 56-57 0.5458959270740067 1103 58-59 0.4862689357576997 1103 60-61 0.5810271652141452 1103 62-63 0.644945998742557 1103 64-65 0.8902498610150609 1103 66-67 0.9153149589743492 1103 68-69 0.8063848688250701 1103 70-71 0.8115093809588316 1103 72-73 0.77427349005821 1103 74-75 0.7360875353463676 1103 76-77 0.837064622685606 1103 78-79 0.698955263674069 1103 80-81 0.8269884447263784 1103 82-83 0.6910951886174992 1103 84-85 0.8331756327876398 1103 86-87 0.8496797107652228 1103 88-89 0.8351448049981123 1103 90-91 0.8819274235075056 1103 92-93 0.837279639570319 1103 94-95 0.708261956611917 1103 96-97 1.1120096600090719 1103 98-99 1.1813876365750993 1103 100-101 1.1234086421012623 1104 1 0.3142247456018801 1104 2 0.22942619110638773 1104 3 0.17108851736941943 1104 4 0.12832879388897567 1104 5 0.2561278210571416 1104 6 0.29907142858727553 1104 7 0.23264672367308492 1104 8 0.37858504997899445 1104 9 0.4053328165434351 1104 10-11 0.503923589188183 1104 12-13 0.5520446039867224 1104 14-15 0.638599524403169 1104 16-17 0.7046319938624208 1104 18-19 0.6357963661842163 1104 20-21 0.6843544478117565 1104 22-23 0.7302241710075847 1104 24-25 0.5924279640629706 1104 26-27 0.5389019149002294 1104 28-29 0.6400627671042614 1104 30-31 0.632836541166121 1104 32-33 0.7498688786370664 1104 34-35 0.6784812879760054 1104 36-37 0.7471760740498468 1104 38-39 0.7584576504508433 1104 40-41 0.8975074297838361 1104 42-43 0.9396150716617768 1104 44-45 0.7931023534620607 1104 46-47 0.6611450585605425 1104 48-49 0.6321583406818903 1104 50-51 0.6753154548730222 1104 52-53 0.8558346407296042 1104 54-55 0.9310731788037714 1104 56-57 0.9582427422900537 1104 58-59 0.8984021571924856 1104 60-61 0.7996052968868312 1104 62-63 0.93854913190183 1104 64-65 1.038437523524724 1104 66-67 0.9989184435058611 1104 68-69 1.0334099892376365 1104 70-71 1.0376447863072578 1104 72-73 1.0303399994182243 1104 74-75 0.8798400017092369 1104 76-77 1.007561044094448 1104 78-79 1.2848167878728098 1104 80-81 1.1286862483795694 1104 82-83 1.1173154638771088 1104 84-85 1.144547525170431 1104 86-87 1.2397546611676802 1104 88-89 1.166736818577295 1104 90-91 1.0737343012137472 1104 92-93 1.2609248292843027 1104 94-95 1.5558266091047663 1104 96-97 1.5497872440816387 1104 98-99 1.6476882679639964 1104 100-101 1.7615780062256157 1105 1 0.424605045334868 1105 2 0.3073174105658829 1105 3 0.2210896749376019 1105 4 0.2757559056791905 1105 5 0.3260981873117146 1105 6 0.30180753391054793 1105 7 0.3366029058588822 1105 8 0.35037549926978784 1105 9 0.47969306719553373 1105 10-11 0.45073410299411165 1105 12-13 0.513679514699632 1105 14-15 0.549430118158476 1105 16-17 0.4285624008633917 1105 18-19 0.587658893387065 1105 20-21 0.5470830675274598 1105 22-23 0.6308857212228958 1105 24-25 0.7305540155207311 1105 26-27 0.8530827038986928 1105 28-29 0.6567931000208702 1105 30-31 0.7141481816717459 1105 32-33 0.6938452796804171 1105 34-35 0.7310796426857635 1105 36-37 0.8207543873958372 1105 38-39 0.7006390491018948 1105 40-41 0.6981979192036647 1105 42-43 0.6721795867950533 1105 44-45 0.6819844898698904 1105 46-47 0.8044377226651349 1105 48-49 0.6309278457057417 1105 50-51 0.8176868876337409 1105 52-53 0.8500321372954929 1105 54-55 0.79487023395032 1105 56-57 0.8110019989769413 1105 58-59 0.9135024411825512 1105 60-61 0.9066271054713653 1105 62-63 0.8675338674360873 1105 64-65 1.0136090365435102 1105 66-67 1.023886803308919 1105 68-69 0.9719917367025595 1105 70-71 1.1384043439618665 1105 72-73 1.0440106867050396 1105 74-75 1.1337429357587148 1105 76-77 1.301015574816308 1105 78-79 1.3321024834169428 1105 80-81 1.4908704251942488 1105 82-83 1.3922272957673414 1105 84-85 1.5564855258186654 1105 86-87 1.5866790025113815 1105 88-89 1.6130545941942778 1105 90-91 1.547132033923539 1105 92-93 1.5379957187596922 1105 94-95 1.7067717635243618 1105 96-97 2.054082400816373 1105 98-99 2.216536371867317 1105 100-101 1.7897618975068124 1106 1 0.29007977683448516 1106 2 0.15238679473061012 1106 3 0.1599792960199231 1106 4 0.21269599862230848 1106 5 0.2954551346407541 1106 6 0.10901999271204232 1106 7 0.3492891670317775 1106 8 0.2517461401309333 1106 9 0.09843123532835563 1106 10-11 0.041732049822726935 1106 12-13 0.2297331334773176 1106 14-15 0.49188874039261066 1106 16-17 0.4594529475392122 1106 18-19 0.20730150977173878 1106 20-21 0.31757697239583393 1106 22-23 0.37065931438895205 1106 24-25 0.2935257437648673 1106 26-27 0.2557105713827923 1106 28-29 0.48437802057284074 1106 30-31 0.08976208799441565 1106 32-33 0.3388761353216623 1106 34-35 0.0663541723019776 1106 36-37 0.3040129738787414 1106 38-39 0.12800482920855671 1106 40-41 0.39154205365255734 1106 42-43 -0.036692729274165004 1106 44-45 0.15864109013924121 1106 46-47 0.25852584896193065 1106 48-49 0.6752459362866503 1106 50-51 0.24690486464892558 1106 52-53 0.4093367125955254 1106 54-55 0.5120135585427832 1106 56-57 -0.3148157853451323 1106 58-59 -0.0018408489715753262 1106 60-61 0.6983255846803473 1106 62-63 0.7595662016031071 1106 64-65 0.7416270116398387 1106 66-67 0.25724095365903565 1106 68-69 0.28450622277093984 1106 70-71 0.1996020811306849 1106 72-73 0.3989422114700929 1106 74-75 0.8233790785435069 1106 76-77 0.8065047532498291 1106 78-79 0.9785890784700406 1106 80-81 1.0719506225935973 1106 82-83 0.6103976478917872 1106 84-85 0.7833625415598178 1106 86-87 0.6988950945072006 1106 88-89 0.7038422878910033 1106 90-91 0.968778057784963 1106 92-93 0.9767009028406939 1106 94-95 0.8972147077297059 1106 96-97 1.0893462252596144 1106 98-99 1.2377043996563266 1106 100-101 0.7693204454611902 1107 1 0.23606810474422701 1107 2 0.14029575632378055 1107 3 0.21148458645816248 1107 4 0.08585311392073436 1107 5 0.13714826973691174 1107 6 0.22997275874411827 1107 7 0.2097263345525775 1107 8 0.05548598307409236 1107 9 0.24210073395459375 1107 10-11 0.4373586695931806 1107 12-13 0.4239599305700139 1107 14-15 0.5014543983360582 1107 16-17 0.7230134709321092 1107 18-19 0.538665137278123 1107 20-21 0.5475095996878778 1107 22-23 0.5207443218910512 1107 24-25 0.45634994143045304 1107 26-27 0.3946220856367759 1107 28-29 0.4850200829668836 1107 30-31 0.5151201425161318 1107 32-33 0.6605126149880718 1107 34-35 0.569944460499336 1107 36-37 0.5539335685902529 1107 38-39 0.6119393856106683 1107 40-41 0.5972443763790309 1107 42-43 0.6839221989200723 1107 44-45 0.5837714024991811 1107 46-47 0.5107206988226025 1107 48-49 0.5047213742336112 1107 50-51 0.6117979841563184 1107 52-53 0.7027896734871959 1107 54-55 0.8085672228776204 1107 56-57 0.908252668833633 1107 58-59 0.679109919749564 1107 60-61 0.5956421243635077 1107 62-63 0.7941998168333413 1107 64-65 0.8185943629132488 1107 66-67 0.8940742902400274 1107 68-69 0.9198379225312614 1107 70-71 0.9848380561107106 1107 72-73 1.0728156793864585 1107 74-75 0.8359721240042504 1107 76-77 1.0395865553114412 1107 78-79 1.1665345762388988 1107 80-81 1.1910249420464325 1107 82-83 0.9315774253621214 1107 84-85 1.0623929688869254 1107 86-87 1.1381639325593795 1107 88-89 1.0566241523076911 1107 90-91 1.1202575293257198 1107 92-93 1.0728714244798532 1107 94-95 1.1407010383285083 1107 96-97 1.1915705476353402 1107 98-99 1.1395485022304221 1107 100-101 0.9581235690606373 1108 1 0.22923509756875404 1108 2 0.08232474183102667 1108 3 0.16824172647915248 1108 4 0.20289746193589764 1108 5 0.24995114748916336 1108 6 0.25126259490888003 1108 7 0.20302284513616087 1108 8 0.17273618869535312 1108 9 0.19768961433501886 1108 10-11 0.33428935868438714 1108 12-13 0.2837302492593281 1108 14-15 0.32898944848874834 1108 16-17 0.34754089655393017 1108 18-19 0.4714588920641276 1108 20-21 0.3791094512624156 1108 22-23 0.2981934601725271 1108 24-25 0.38257634225482917 1108 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2316 50-51 -1.2597155872982029 2316 52-53 -1.244140852293114 2316 54-55 -1.3724572675115354 2316 56-57 -1.2305018277326312 2316 58-59 -1.0599974891381905 2316 60-61 -1.1426413682421384 2316 62-63 -1.2563779238010895 2316 64-65 -1.382851351268652 2316 66-67 -1.3549051254788758 2316 68-69 -1.546069021257118 2316 70-71 -1.7381884509202834 2316 72-73 -1.4694788411614859 2316 74-75 -1.4273717770167877 2316 76-77 -1.831788031367818 2316 78-79 -1.95424637285452 2316 80-81 -2.216755934375211 2316 82-83 -2.340664680666002 2316 84-85 -2.3185910978257667 2316 86-87 -2.091986679700348 2316 88-89 -2.12578518964936 2316 90-91 -2.516764199535878 2316 92-93 -2.6136724874419492 2316 94-95 -3.11044217957782 2316 96-97 -3.07706567561236 2316 98-99 -3.0881303611829694 2316 100-101 -3.055808973361513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9431.0 3 3136.0 4 3084.0 5 3601.0 6 4050.0 7 5119.0 8 6312.0 9 7928.0 10 9761.0 11 12785.0 12 15802.0 13 19189.0 14 22143.0 15 26669.0 16 32078.0 17 38297.0 18 35196.0 19 30559.0 20 32411.0 21 34833.0 22 38670.0 23 42815.0 24 48654.0 25 55005.0 26 61807.0 27 70944.0 28 81109.0 29 93845.0 30 109438.0 31 128005.0 32 151817.0 33 184159.0 34 225673.0 35 280259.0 36 335167.0 37 341720.0 38 164987.0 39 8655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03668567618945 18.418443741722747 14.500211717433805 23.044658864654 2 15.599590654898055 20.11291980147412 40.10608054950648 24.181408994121345 3 19.250770301378047 24.522045175862484 29.32221054579654 26.904973976962932 4 12.92998888116848 16.442774815882686 36.259873284283486 34.36736301866535 5 14.18762187756139 37.196300877283846 33.121678875927515 15.49439836922725 6 32.34212221618647 35.35197012227101 17.417000497048367 14.888907164494153 7 29.32408823477466 32.65309506190773 19.858699503418972 18.164117199898644 8 26.8715070710129 34.48888119941392 19.491413319492278 19.1481984100809 9 27.389906039745952 16.588819994874697 19.69168176564109 36.32959219973826 10-11 25.55153639900478 26.507709485766284 26.5476026089706 21.393151506258334 12-13 26.606674211693154 23.84833487226395 27.16451102207227 22.380479893970627 14-15 23.237178880659297 24.957588989231127 25.907202744005463 25.89802938610411 16-17 22.728150944266776 28.469016111218448 26.208107083226395 22.594725861288378 18-19 22.594061490312225 26.43514224442632 29.1942720839433 21.77652418131816 20-21 23.39807850367679 25.609668814022008 29.109347819463498 21.882904862837698 22-23 23.052807847218304 25.476027965736186 29.041549387287525 22.42961479975798 24-25 22.84147317753254 25.73213351232464 29.110843797877305 22.315549512265516 26-27 22.35886095780242 26.269437412496266 29.012173633373273 22.359527996328044 28-29 22.273351424249533 26.349756551407232 29.235662095624665 22.14122992871857 30-31 22.274725058763693 25.95865004966314 29.095460145352803 22.671164746220363 32-33 22.10360071933627 26.13072292718523 28.95552027135187 22.81015608212663 34-35 22.940225297478094 26.15755535127033 28.608683596459233 22.293535754792345 36-37 22.815450408574097 25.8534051026289 28.601611005442546 22.72953348335445 38-39 22.36937271906567 26.218347178368546 28.77908543285778 22.633194669708004 40-41 22.689203615683244 25.936548727930308 28.803840117891273 22.570407538495175 42-43 22.598317241881475 25.912521305726287 28.594488023645052 22.894673428747183 44-45 22.494818250928372 26.275012987031708 28.360830283505596 22.869338478534324 46-47 22.61234725051834 26.53032392782991 27.874169781992652 22.983159039659093 48-49 22.789693051489515 26.467446037105113 27.8472881292713 22.895572782134074 50-51 22.771736838899795 26.66311814312995 27.575577605092576 22.989567412877683 52-53 23.10284569092055 26.601514256564464 27.478487848071246 22.817152204443737 54-55 22.486751225770647 26.602637681838416 27.73865362541602 23.17195746697492 56-57 22.63360240170325 26.96533586093619 27.57239302662723 22.828668710733332 58-59 22.843979906742053 26.293785056202555 27.6597661793526 23.20246885770279 60-61 22.73798745112513 26.458767055743305 27.934402429037725 22.868843064093838 62-63 22.6359444878813 26.63546661241615 27.787809598701735 22.94077930100082 64-65 22.376319084113618 26.377213549456147 27.835098358753402 23.411369007676832 66-67 22.409528745093347 26.679596108409818 27.608076101951706 23.30279904454513 68-69 22.53187389012371 26.419105503905232 27.788031760819017 23.260988845152042 70-71 22.408776541772056 26.614530222304406 27.56887514828284 23.4078180876407 72-73 22.68996145370083 26.468158392344666 27.467788572792095 23.374091581162414 74-75 22.481093773908192 26.751726436131552 27.35006088957351 23.41711890038675 76-77 22.634127757335385 26.526808971682087 27.413935597627358 23.425127673355167 78-79 22.680320422290315 26.66867990672308 27.316346199931314 23.33465347105529 80-81 22.519980821438477 27.01216786394537 27.176836103119545 23.291015211496614 82-83 22.73205495663618 26.79273853975466 27.010363964919748 23.464842538689414 84-85 22.647423163376082 26.49828601287405 27.10424014738938 23.75005067636049 86-87 22.777500273899932 26.398223110234632 27.259296019513208 23.564980596352232 88-89 22.69496195820269 26.4053259237203 27.202084025761575 23.697628092315433 90-91 22.60690816153649 26.523425531071226 27.184989059554237 23.684677247838046 92-93 22.928689644209676 26.097484093669422 27.205209551926558 23.768616710194344 94-95 22.697525316270877 26.082419517710125 27.20088153149694 24.01917363452206 96-97 22.929641247708354 25.943105701704816 27.12885871272689 23.99839433785994 98-99 22.778525691279327 25.949354541637152 26.84740942587276 24.424710341210755 100-101 23.249402096772332 25.467576191190556 27.132996324590053 24.15002538744706 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1378.0 1 923.0 2 690.5 3 924.5 4 827.0 5 804.0 6 1288.0 7 2713.5 8 4391.0 9 4928.5 10 4160.0 11 3368.5 12 3397.5 13 3638.0 14 3788.0 15 3987.0 16 4129.0 17 4281.0 18 4516.0 19 4855.5 20 5280.5 21 5887.5 22 6791.0 23 7905.5 24 9306.5 25 11104.5 26 13472.5 27 16336.5 28 19054.5 29 22065.5 30 26217.0 31 30574.5 32 35492.5 33 40226.0 34 43849.5 35 47647.5 36 52768.5 37 58364.5 38 62913.0 39 65798.0 40 68713.5 41 76101.0 42 84518.0 43 90836.0 44 95658.5 45 99031.0 46 99755.5 47 102793.5 48 111180.5 49 115457.5 50 115061.0 51 112694.5 52 108257.0 53 106249.5 54 104125.0 55 95588.5 56 85153.5 57 76622.5 58 68947.0 59 60723.5 60 51453.0 61 42901.5 62 35525.5 63 27743.0 64 21544.0 65 17364.5 66 13520.5 67 10924.5 68 8398.0 69 6063.5 70 4809.5 71 3952.0 72 3360.5 73 2861.5 74 2272.0 75 1870.5 76 1364.5 77 863.5 78 542.5 79 329.0 80 214.5 81 146.0 82 84.0 83 48.5 84 30.0 85 22.0 86 19.0 87 13.5 88 11.5 89 9.5 90 9.0 91 5.5 92 3.0 93 4.0 94 2.0 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006774498912296544 2 0.10377955780539387 3 0.11336475307491983 4 0.11671596796238569 5 0.15862417134004994 6 0.09898696017063088 7 0.026737649962361894 8 0.027314203061280747 9 0.02313419309411905 10-11 0.052322193726886075 12-13 0.055565304908304636 14-15 0.027908773444540815 16-17 0.032323008108138304 18-19 0.03783629711654985 20-21 0.06131281861315197 22-23 0.03360223529636451 24-25 0.051223139382072014 26-27 0.05981738401283119 28-29 0.055114872799774284 30-31 0.06952870027274566 32-33 0.05206995174610908 34-35 0.06333075445936795 36-37 0.05417797401403114 38-39 0.032196887117749796 40-41 0.03716965759592492 42-43 0.03306171676612808 44-45 0.008269933512617325 46-47 0.014053481786147086 48-49 0.009603212553867176 50-51 0.009008642170607107 52-53 0.010305886643174531 54-55 0.011819338527836523 56-57 0.014972363287549011 58-59 0.01983703005967685 60-61 0.024539541272733757 62-63 0.011945459518225024 64-65 0.009765368112938103 66-67 0.011134681722870384 68-69 0.011188733575894028 70-71 0.030773521654793877 72-73 0.028359205553071174 74-75 0.02985464015339195 76-77 0.023422469643578477 78-79 0.0054051853023642646 80-81 0.005026822331198766 82-83 0.005819582842212191 84-85 0.005423202586705478 86-87 0.013585032393275518 88-89 0.0381606082346917 90-91 0.03614267238847571 92-93 0.029710501878662237 94-95 0.04698907756188667 96-97 0.04250277376092433 98-99 0.026233166000807894 100-101 0.07527621397759299 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2775113.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.17222426340505 #Duplication Level Percentage of deduplicated Percentage of total 1 72.4379320918418 32.721825136299834 2 14.032869526881544 12.677918586547914 3 5.2743905465835415 7.147678578691657 4 2.596219774265601 4.691080875208502 5 1.4762543356024833 3.33428459588297 6 0.8820705017271476 2.3907051912091752 7 0.6299607463098041 1.991970967661394 8 0.42358245999049515 1.5307329501388351 9 0.3184755267034044 1.2947623123187004 >10 1.6726372191075436 13.806245197260331 >50 0.14412047566975167 4.495073336503959 >100 0.10147517242155503 8.889998870295116 >500 0.006968089535211849 2.152769387004006 >1k 0.002803254410711072 1.7693360576428019 >5k 8.009298316317349E-5 0.2616521832339429 >10k+ 1.6018596632634698E-4 0.8439657741009657 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13154 0.47399871644866354 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10173 0.3665796672063444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7232 0.2606020007113224 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6034568682428426E-5 0.0 0.0 0.0 0.0 3 3.6034568682428426E-5 0.0 0.0 0.0 0.0 4 7.206913736485685E-5 0.0 0.0 0.0 0.0 5 1.0810370604728529E-4 0.0 0.0 0.0 0.0 6 1.0810370604728529E-4 0.0 0.0 0.0 0.0 7 1.0810370604728529E-4 0.0 0.0 0.0 0.0 8 1.0810370604728529E-4 0.0 0.0 0.0 0.0 9 1.0810370604728529E-4 0.0 0.0 0.0 0.0 10-11 1.0810370604728529E-4 0.0 0.0 0.0 0.0 12-13 1.8017284341214214E-4 0.0 0.0 0.0 0.0 14-15 3.2431111814185587E-4 0.0 0.0 0.0 0.0 16-17 3.603456868242843E-4 0.0 0.0 1.8017284341214213E-5 0.0 18-19 4.324148241891411E-4 0.0 0.0 3.6034568682428426E-5 0.0 20-21 4.6844939287156955E-4 0.0 0.0 1.0810370604728527E-4 0.0 22-23 5.04483961553998E-4 0.0 0.0 2.3422469643578477E-4 0.0 24-25 6.486222362837117E-4 0.0 0.0 5.945703832600691E-4 0.0 26-27 6.486222362837117E-4 0.0 0.0 0.0015494864533444224 0.0 28-29 7.747432266722112E-4 0.0 0.0 0.004180009967161697 0.0 30-31 8.287950796958539E-4 0.0 0.0 0.014107533639170729 0.0 32-33 8.287950796958539E-4 0.0 0.0 0.033548183443340866 0.0 34-35 9.188815014019248E-4 0.0 0.0 0.06136687046617561 0.0 36-37 9.729333544255675E-4 0.0 0.0 0.10950905422589999 0.0 38-39 0.0011891407665201381 0.0 0.0 0.18294750520068914 0.0 40-41 0.0012431926195437806 0.0 0.0 0.2671602922115244 0.0 42-43 0.0013152617569086376 0.0 0.0 0.35094066439817045 0.0 44-45 0.0014053481786147086 0.0 0.0 0.4531887530345611 0.0 46-47 0.0014954346003207798 0.0 0.0 0.5802286249244626 0.0 48-49 0.001513451884661994 0.0 0.0 0.7120070425961033 0.0 50-51 0.001513451884661994 0.0 0.0 0.8585596334275397 0.0 52-53 0.001513451884661994 0.0 0.0 1.0033645476778783 0.0 54-55 0.0015314691690032083 0.0 0.0 1.1639165684424382 1.8017284341214213E-5 56-57 0.0015855210220268507 0.0 0.0 1.3462695032598673 3.6034568682428426E-5 58-59 0.0015855210220268507 0.0 0.0 1.5458109273388148 3.6034568682428426E-5 60-61 0.0015855210220268507 0.0 0.0 1.7663605049596178 3.6034568682428426E-5 62-63 0.0015855210220268507 0.0 0.0 1.9824958479168235 3.6034568682428426E-5 64-65 0.0015855210220268507 0.0 0.0 2.209946045440312 3.6034568682428426E-5 66-67 0.0015855210220268507 0.0 0.0 2.477592804329049 3.6034568682428426E-5 68-69 0.0016575901593917078 0.0 0.0 2.7653288352582397 3.6034568682428426E-5 70-71 0.0016575901593917078 0.0 0.0 3.0796043260220394 3.6034568682428426E-5 72-73 0.001675607443732922 0.0 0.0 3.3985462934302135 3.6034568682428426E-5 74-75 0.001693624728074136 0.0 0.0 3.7284968215708694 3.6034568682428426E-5 76-77 0.001693624728074136 0.0 0.0 4.078320414339885 3.6034568682428426E-5 78-79 0.001693624728074136 0.0 0.0 4.464430817772105 3.6034568682428426E-5 80-81 0.001855780287145064 0.0 0.0 4.8855488046793045 3.6034568682428426E-5 82-83 0.0018737975714862782 0.0 0.0 5.307441534813178 3.6034568682428426E-5 84-85 0.0019098321401687067 0.0 0.0 5.732397203285055 5.4051853023642636E-5 86-87 0.001945866708851135 0.0 0.0 6.1760367956187725 7.206913736485685E-5 88-89 0.001945866708851135 0.0 0.0 6.648972492291305 7.206913736485685E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6435 0.0 53.288486 1 GTATCAA 11930 0.0 42.178574 1 ATCAACG 15590 0.0 32.061775 3 TATCAAC 15725 0.0 31.695791 2 TCAACGC 15885 0.0 31.556175 4 CAACGCA 16080 0.0 31.084768 5 AACGCAG 16790 0.0 29.862564 6 GTACATG 14090 0.0 26.430016 1 ACGCAGA 19030 0.0 26.171713 7 CGCAGAG 19200 0.0 25.865263 8 TACATGG 14525 0.0 24.884472 2 ACATGGG 14735 0.0 23.884302 3 GCAGAGT 20220 0.0 23.666525 9 GAGTACT 10790 0.0 22.239088 12-13 AGTACTT 11110 0.0 21.577152 12-13 AGAGTAC 18310 0.0 21.28158 10-11 GTACTTT 11580 0.0 20.739437 14-15 CATGGGG 6710 0.0 20.696234 4 GGACCGA 785 0.0 20.584229 6 CAGAGTA 19810 0.0 20.149912 10-11 >>END_MODULE